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The map label for this gene is ascF [H]

Identifier: 91212074

GI number: 91212074

Start: 2998014

End: 2999471

Strand: Direct

Name: ascF [H]

Synonym: UTI89_C3077

Alternate gene names: 91212074

Gene position: 2998014-2999471 (Clockwise)

Preceding gene: 91212070

Following gene: 91212075

Centisome position: 59.18

GC content: 56.04

Gene sequence:

>1458_bases
ATGGCCAAAAATTATGCGGCGCTGGCAAGCTCGGTGATAACGGCACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCA
CTGTATGACGCGTTTGCGCTTTGTTATCAAAGATGACCAGCTTATCGATAGTCCGACGTTGAAAACCATCTCCGGCGTGC
TCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATAGGTAATACGGTTTCGCAGGCCTTTCAGGAAGTCGTCAGC
CTGCTGCCGGGAGACTTGCAACCCGCGCCTCCCGTGGGTAAACCAAAGCTCACGCTACGTCGCATTGGTGCGGGGGTCCT
CGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGTCAAACTGCTGGCGATGATCC
TCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAATCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTC
CTGCCGCTGATGGTCGCGGCTTCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATTGCGGGTGTGCT
GGTACATCCGAGTTTTATTGAGCTGATGGCGAAAGCGGCCCTGGGTGAACATGTTGAATTTGCCCTGATTCCGGTCACCG
CGGTGAAATACACCTACACAGTGATCCCGGCGCTGGTGATGACCTGGTGCCTGTCATATATCGAACGCTGGGTGGACAGC
ATTACGCCAGCGGTGACGAAAAACTTCCTCAAGCCGATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGAT
TGGCCCGATTGGTATCTGGATCGGTAGCGCCATTTCGGCGCTGGTTTACACCATTCATGGTTATCTGGGCTGGCTTTCAG
TCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACAGGGATGCACCGCGTCTTTACGCCAACCATCATTCAGACC
ATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTTCATCACTGGC
GGTGGCATGGAAAACGAAAAACCCGGAACTGCGCCAGACGGCGCTGGCTGCGGCAGCATCAGCCATTATGGCGGGGATTT
CCGAACCCGCGTTATACGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGC
GCGGTTGCCGGTATGGCGGGGCTTGCCAGCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCC
GGCGAATCCAATGAGCATCGTCTGGGTGTTCGCCGTCATGGCGCTGGCGGTCGTGCTGTCGTTCATCCTCACACTGTTGC
TCGGCTTTGAGGATATTCCTGTTGAGGAAGCGACTGCCGAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAA
GAAGTAAGTCTTAATTGA

Upstream 100 bases:

>100_bases
TGTGAAACCGGTTTCCCAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTAC
TGATAACAGGATAAAATCCG

Downstream 100 bases:

>100_bases
GGATGAAAATGTCAGTATTTCCGCAAGGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGCGCGTACCGT
GAAGGCGGCAAAGGGCTGAC

Product: PTS system cellobiose/arbutin/salicin-specific transporter subunits IIBC

Products: NA

Alternate protein names: EIIBC-Asc; EII-Asc; Arbutin-, cellobiose-, and salicin-specific phosphotransferase enzyme IIB component; PTS system arbutin-, cellobiose-, and salicin-specific EIIB component; Arbutin, cellobiose, and salicin permease IIC component; PTS system arbutin-, cellobiose-, and salicin-specific EIIC component [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MAKNYAALASSVITALGGVDNISAVTHCMTRLRFVIKDDQLIDSPTLKTISGVLGVVRSDNQCQVIIGNTVSQAFQEVVS
LLPGDLQPAPPVGKPKLTLRRIGAGVLDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLIILNVIGDGAFFF
LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAALGEHVEFALIPVTAVKYTYTVIPALVMTWCLSYIERWVDS
ITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGSAISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQT
IAETGKEGMVMPSEIGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIASLISGFICG
AVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFILTLLLGFEDIPVEEATAEARKHQSVQPTVAK
EVSLN

Sequences:

>Translated_485_residues
MAKNYAALASSVITALGGVDNISAVTHCMTRLRFVIKDDQLIDSPTLKTISGVLGVVRSDNQCQVIIGNTVSQAFQEVVS
LLPGDLQPAPPVGKPKLTLRRIGAGVLDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLIILNVIGDGAFFF
LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAALGEHVEFALIPVTAVKYTYTVIPALVMTWCLSYIERWVDS
ITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGSAISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQT
IAETGKEGMVMPSEIGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIASLISGFICG
AVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFILTLLLGFEDIPVEEATAEARKHQSVQPTVAK
EVSLN
>Mature_484_residues
AKNYAALASSVITALGGVDNISAVTHCMTRLRFVIKDDQLIDSPTLKTISGVLGVVRSDNQCQVIIGNTVSQAFQEVVSL
LPGDLQPAPPVGKPKLTLRRIGAGVLDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLIILNVIGDGAFFFL
PLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAALGEHVEFALIPVTAVKYTYTVIPALVMTWCLSYIERWVDSI
TPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGSAISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTI
AETGKEGMVMPSEIGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIASLISGFICGA
VAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFILTLLLGFEDIPVEEATAEARKHQSVQPTVAKE
VSLN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI48994906, Length=485, Percent_Identity=97.5257731958763, Blast_Score=918, Evalue=0.0,
Organism=Escherichia coli, GI1790159, Length=459, Percent_Identity=31.5904139433551, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI2367362, Length=432, Percent_Identity=27.0833333333333, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1788769, Length=488, Percent_Identity=25.4098360655738, Blast_Score=102, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 50974; Mature: 50842

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNYAALASSVITALGGVDNISAVTHCMTRLRFVIKDDQLIDSPTLKTISGVLGVVRSD
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC
NQCQVIIGNTVSQAFQEVVSLLPGDLQPAPPVGKPKLTLRRIGAGVLDALIGTMSPLIPA
CCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IIGGSMVKLLAMILEMSGVLTKGSPTLIILNVIGDGAFFFLPLMVAASAAIKFKTNMSLA
HHCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHEEECCCHHH
IAIAGVLVHPSFIELMAKAALGEHVEFALIPVTAVKYTYTVIPALVMTWCLSYIERWVDS
HHHHHHHHCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGSAISALVYTIHGYLGWLSVAIMGAL
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSEIGANLSLGGSSLAVAWKTKNPELRQT
HHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCEEECCCEEEEEEECCCCHHHHH
ALAAAASAIMAGISEPALYGVAIRLKRPLIASLISGFICGAVAGMAGLASHSMAAPGLFT
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
SVQFFDPANPMSIVWVFAVMALAVVLSFILTLLLGFEDIPVEEATAEARKHQSVQPTVAK
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHH
EVSLN
HCCCC
>Mature Secondary Structure 
AKNYAALASSVITALGGVDNISAVTHCMTRLRFVIKDDQLIDSPTLKTISGVLGVVRSD
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC
NQCQVIIGNTVSQAFQEVVSLLPGDLQPAPPVGKPKLTLRRIGAGVLDALIGTMSPLIPA
CCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IIGGSMVKLLAMILEMSGVLTKGSPTLIILNVIGDGAFFFLPLMVAASAAIKFKTNMSLA
HHCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHEEECCCHHH
IAIAGVLVHPSFIELMAKAALGEHVEFALIPVTAVKYTYTVIPALVMTWCLSYIERWVDS
HHHHHHHHCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGSAISALVYTIHGYLGWLSVAIMGAL
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSEIGANLSLGGSSLAVAWKTKNPELRQT
HHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCEEECCCEEEEEEECCCCHHHHH
ALAAAASAIMAGISEPALYGVAIRLKRPLIASLISGFICGAVAGMAGLASHSMAAPGLFT
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
SVQFFDPANPMSIVWVFAVMALAVVLSFILTLLLGFEDIPVEEATAEARKHQSVQPTVAK
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHH
EVSLN
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]