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The map label for this gene is gidA [H]

Identifier: 89898690

GI number: 89898690

Start: 1038380

End: 1040215

Strand: Direct

Name: gidA [H]

Synonym: CF0883

Alternate gene names: 89898690

Gene position: 1038380-1040215 (Clockwise)

Preceding gene: 89898689

Following gene: 89898691

Centisome position: 89.04

GC content: 42.54

Gene sequence:

>1836_bases
ATGTGGACTCACCCAATTAGTTATGATGTTATTGTAGTTGGGGCTGGGCACGCAGGTTGCGAGGCCGCATTTTGCTCTGC
AAAAATGGGAGCCTCCGTACTCATTTTAACGTCAAACTTAGATACTATTGCTAAGTTAAGTTGTAATCCTGCCGTTGGCG
GTATCGGCAAAGGGCATATTGTTCGAGAAATCGACGCCCTAGGCGGTATTATGGCTGAGATTACCGATCGGTCTGGGATA
CAATTTCGCATCTTAAATCAGACCAAGGGTCCTGCTGTCCGAGCACCAAGAGCTCAAGTTGATAAGCAAATGTATCATAT
TCATATGAAACGCTTATTAGAGAGCACCCCTGGCCTACATATCATGCAAGGTACGGTCGAGTCCTTACTAGACAAAGAAA
ACGTCATTCAGGGCGTAACAACAAAAGAGGGTATTGCATACCTGGGGAAAACAGTTATTCTTTCTTCTGGGACGTTTATG
CGCGGTCTCATTCATATTGGCGATCGTAGTTTCTCTGGAGGACGTCTTGGAGATCCTGCAGCTACAGGGCTGTCAGCAGC
TCTAAAAGAGCGGGGTTTCCCGATAAGTAGACTAAAAACAGGTACCCCCCCTCGTTTACTTGCTTCTTCCATAGACTTTT
CAGTAACAGAAGAACAACCTGGGGATCCTGGTGTAGGATTCGTCCACAGAGATGAACCGTTTGTCCCTCCCCTACCACAA
GTATCGTGTTACATTACTCACACCACTGAGAAGACTAAAGAGATCATTACGGCTAATATTCGGCGTTCAGCTCTTTATGG
AGGGCGTATTGAAGGGATTGGCCCTCGGTATTGCCCATCTATTGAAGATAAAATTGTTAAATTTGCAGATAAAGAACGCC
ATCATATTTTCATAGAACCTGAGGGAATTTACACCCAAGAGGTCTACGTTAACGGCTTATCCACATCCATGCCTTTTGAT
GTGCAGTACGAGATGATTCGTTCGGTTCGTGGTTTAGAAAACGCAATCATTACCCGTCCTGCTTACGCTATTGAGTATGA
TTACGTCCACGGGAATGTCATCTATCCCACTTTAGAAAGCAAGACCATAGAAGGCCTATTTCTTTGCGGGCAAATCAACG
GAACAACTGGATACGAAGAAGCGGCGGCCCAAGGTCTTATTGCGGGTATTAATGCGGTAAATAAGGTGCTTAGGAAGCCA
GATTTTATTCCTTCAAGGCAGGAATCTTATATTGGAGTTATGTTAGATGATCTTACAACTCAAGTATTGGATGAACCTTA
CCGCATGTTTACGGGAAGAGCAGAACACCGCCTTCTTCTAAGACAAGATAATGCCTGCTTGCGCTTATCTCACTATGGTC
GTGATCTGGGGTTACTAAGTAAAGAGCGTTATGAGCTTTTCGAACATCAAAAGCAAATTATAGAAGAAGAAAAACTTCGT
TTAAGTAAGACATTCAAAAAATACGGGAATTCCGTTGTTTCCCTAGCCAAGGCTTTATGTCGTCCTGAAGTATCCTATGA
TACTCTTAGAGAGGTATTCCCAGAAGAAGTGCGTGACTTTGGTTCTATTCTCAATGCTTCTTTGGAAATGGAAATCAAGT
ATGCAGGATATATAGAGCGACAGAAATCTTTGATTCATAGTTTATCGAAATCAGAAAATATGACGATTCCTGAAGATATA
GATTATCAAAGTATTTCCTCTTTAAGTTTAGAAGCCAGAGAAAAACTAGCTAAATTTACCCCAAGGACGATAGGGTCAGC
ATCGAGAATATCGGGCATAGCTAATGCTGATATTCAAGTATTGATGGTTGCTGTAAAAAAACATGCGCATAAATAA

Upstream 100 bases:

>100_bases
ATGTCATCTGTTAAGAAAAAACGAAGACTAAAAATCGCTAAACATAAGCGTAAAAAAAGACGTCGAAGAGACCGTCATAA
AAATAAATAATTGCTAGTTT

Downstream 100 bases:

>100_bases
GTGTATTTTCTTAAATCTATCTGGGAAAACTATCTTTGAGCAGCTGCAAATAGAAGAAGCCCTTCTTCGCAACAGCAGAG
AAAATTACTGTATTATTAAT

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 611; Mature: 611

Protein sequence:

>611_residues
MWTHPISYDVIVVGAGHAGCEAAFCSAKMGASVLILTSNLDTIAKLSCNPAVGGIGKGHIVREIDALGGIMAEITDRSGI
QFRILNQTKGPAVRAPRAQVDKQMYHIHMKRLLESTPGLHIMQGTVESLLDKENVIQGVTTKEGIAYLGKTVILSSGTFM
RGLIHIGDRSFSGGRLGDPAATGLSAALKERGFPISRLKTGTPPRLLASSIDFSVTEEQPGDPGVGFVHRDEPFVPPLPQ
VSCYITHTTEKTKEIITANIRRSALYGGRIEGIGPRYCPSIEDKIVKFADKERHHIFIEPEGIYTQEVYVNGLSTSMPFD
VQYEMIRSVRGLENAIITRPAYAIEYDYVHGNVIYPTLESKTIEGLFLCGQINGTTGYEEAAAQGLIAGINAVNKVLRKP
DFIPSRQESYIGVMLDDLTTQVLDEPYRMFTGRAEHRLLLRQDNACLRLSHYGRDLGLLSKERYELFEHQKQIIEEEKLR
LSKTFKKYGNSVVSLAKALCRPEVSYDTLREVFPEEVRDFGSILNASLEMEIKYAGYIERQKSLIHSLSKSENMTIPEDI
DYQSISSLSLEAREKLAKFTPRTIGSASRISGIANADIQVLMVAVKKHAHK

Sequences:

>Translated_611_residues
MWTHPISYDVIVVGAGHAGCEAAFCSAKMGASVLILTSNLDTIAKLSCNPAVGGIGKGHIVREIDALGGIMAEITDRSGI
QFRILNQTKGPAVRAPRAQVDKQMYHIHMKRLLESTPGLHIMQGTVESLLDKENVIQGVTTKEGIAYLGKTVILSSGTFM
RGLIHIGDRSFSGGRLGDPAATGLSAALKERGFPISRLKTGTPPRLLASSIDFSVTEEQPGDPGVGFVHRDEPFVPPLPQ
VSCYITHTTEKTKEIITANIRRSALYGGRIEGIGPRYCPSIEDKIVKFADKERHHIFIEPEGIYTQEVYVNGLSTSMPFD
VQYEMIRSVRGLENAIITRPAYAIEYDYVHGNVIYPTLESKTIEGLFLCGQINGTTGYEEAAAQGLIAGINAVNKVLRKP
DFIPSRQESYIGVMLDDLTTQVLDEPYRMFTGRAEHRLLLRQDNACLRLSHYGRDLGLLSKERYELFEHQKQIIEEEKLR
LSKTFKKYGNSVVSLAKALCRPEVSYDTLREVFPEEVRDFGSILNASLEMEIKYAGYIERQKSLIHSLSKSENMTIPEDI
DYQSISSLSLEAREKLAKFTPRTIGSASRISGIANADIQVLMVAVKKHAHK
>Mature_611_residues
MWTHPISYDVIVVGAGHAGCEAAFCSAKMGASVLILTSNLDTIAKLSCNPAVGGIGKGHIVREIDALGGIMAEITDRSGI
QFRILNQTKGPAVRAPRAQVDKQMYHIHMKRLLESTPGLHIMQGTVESLLDKENVIQGVTTKEGIAYLGKTVILSSGTFM
RGLIHIGDRSFSGGRLGDPAATGLSAALKERGFPISRLKTGTPPRLLASSIDFSVTEEQPGDPGVGFVHRDEPFVPPLPQ
VSCYITHTTEKTKEIITANIRRSALYGGRIEGIGPRYCPSIEDKIVKFADKERHHIFIEPEGIYTQEVYVNGLSTSMPFD
VQYEMIRSVRGLENAIITRPAYAIEYDYVHGNVIYPTLESKTIEGLFLCGQINGTTGYEEAAAQGLIAGINAVNKVLRKP
DFIPSRQESYIGVMLDDLTTQVLDEPYRMFTGRAEHRLLLRQDNACLRLSHYGRDLGLLSKERYELFEHQKQIIEEEKLR
LSKTFKKYGNSVVSLAKALCRPEVSYDTLREVFPEEVRDFGSILNASLEMEIKYAGYIERQKSLIHSLSKSENMTIPEDI
DYQSISSLSLEAREKLAKFTPRTIGSASRISGIANADIQVLMVAVKKHAHK

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=629, Percent_Identity=42.4483306836248, Blast_Score=467, Evalue=1e-131,
Organism=Homo sapiens, GI19882217, Length=654, Percent_Identity=40.8256880733945, Blast_Score=453, Evalue=1e-127,
Organism=Homo sapiens, GI183227703, Length=669, Percent_Identity=39.9103139013453, Blast_Score=447, Evalue=1e-125,
Organism=Escherichia coli, GI2367273, Length=621, Percent_Identity=48.9533011272142, Blast_Score=602, Evalue=1e-173,
Organism=Caenorhabditis elegans, GI17534255, Length=621, Percent_Identity=38.1642512077295, Blast_Score=424, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6321202, Length=632, Percent_Identity=40.8227848101266, Blast_Score=475, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24658174, Length=630, Percent_Identity=41.5873015873016, Blast_Score=457, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 67601; Mature: 67601

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWTHPISYDVIVVGAGHAGCEAAFCSAKMGASVLILTSNLDTIAKLSCNPAVGGIGKGHI
CCCCCCCEEEEEEECCCCCCHHHHHHHCCCCEEEEEECCCCHHHEECCCCCCCCCCCCHH
VREIDALGGIMAEITDRSGIQFRILNQTKGPAVRAPRAQVDKQMYHIHMKRLLESTPGLH
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCE
IMQGTVESLLDKENVIQGVTTKEGIAYLGKTVILSSGTFMRGLIHIGDRSFSGGRLGDPA
EHHHHHHHHHCHHHHHCCCCCHHHHHHHCCEEEEECCHHHHHHHEECCCCCCCCCCCCHH
ATGLSAALKERGFPISRLKTGTPPRLLASSIDFSVTEEQPGDPGVGFVHRDEPFVPPLPQ
HHHHHHHHHHCCCCHHHCCCCCCHHHHHHCCCCEECCCCCCCCCCEEEECCCCCCCCCCC
VSCYITHTTEKTKEIITANIRRSALYGGRIEGIGPRYCPSIEDKIVKFADKERHHIFIEP
EEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECC
EGIYTQEVYVNGLSTSMPFDVQYEMIRSVRGLENAIITRPAYAIEYDYVHGNVIYPTLES
CCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCEEEEECCC
KTIEGLFLCGQINGTTGYEEAAAQGLIAGINAVNKVLRKPDFIPSRQESYIGVMLDDLTT
CCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHH
QVLDEPYRMFTGRAEHRLLLRQDNACLRLSHYGRDLGLLSKERYELFEHQKQIIEEEKLR
HHHHHHHHHHCCCCHHEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LSKTFKKYGNSVVSLAKALCRPEVSYDTLREVFPEEVRDFGSILNASLEMEIKYAGYIER
HHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHCHHHHHHHHHHHCCCEEEEEEEHHHHHH
QKSLIHSLSKSENMTIPEDIDYQSISSLSLEAREKLAKFTPRTIGSASRISGIANADIQV
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCHHH
LMVAVKKHAHK
HHHHHHHHCCC
>Mature Secondary Structure
MWTHPISYDVIVVGAGHAGCEAAFCSAKMGASVLILTSNLDTIAKLSCNPAVGGIGKGHI
CCCCCCCEEEEEEECCCCCCHHHHHHHCCCCEEEEEECCCCHHHEECCCCCCCCCCCCHH
VREIDALGGIMAEITDRSGIQFRILNQTKGPAVRAPRAQVDKQMYHIHMKRLLESTPGLH
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCE
IMQGTVESLLDKENVIQGVTTKEGIAYLGKTVILSSGTFMRGLIHIGDRSFSGGRLGDPA
EHHHHHHHHHCHHHHHCCCCCHHHHHHHCCEEEEECCHHHHHHHEECCCCCCCCCCCCHH
ATGLSAALKERGFPISRLKTGTPPRLLASSIDFSVTEEQPGDPGVGFVHRDEPFVPPLPQ
HHHHHHHHHHCCCCHHHCCCCCCHHHHHHCCCCEECCCCCCCCCCEEEECCCCCCCCCCC
VSCYITHTTEKTKEIITANIRRSALYGGRIEGIGPRYCPSIEDKIVKFADKERHHIFIEP
EEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECC
EGIYTQEVYVNGLSTSMPFDVQYEMIRSVRGLENAIITRPAYAIEYDYVHGNVIYPTLES
CCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCEEEEECCC
KTIEGLFLCGQINGTTGYEEAAAQGLIAGINAVNKVLRKPDFIPSRQESYIGVMLDDLTT
CCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHH
QVLDEPYRMFTGRAEHRLLLRQDNACLRLSHYGRDLGLLSKERYELFEHQKQIIEEEKLR
HHHHHHHHHHCCCCHHEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LSKTFKKYGNSVVSLAKALCRPEVSYDTLREVFPEEVRDFGSILNASLEMEIKYAGYIER
HHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHCHHHHHHHHHHHCCCEEEEEEEHHHHHH
QKSLIHSLSKSENMTIPEDIDYQSISSLSLEAREKLAKFTPRTIGSASRISGIANADIQV
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCHHH
LMVAVKKHAHK
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA