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The map label for this gene is proS [H]

Identifier: 89898569

GI number: 89898569

Start: 905619

End: 907352

Strand: Direct

Name: proS [H]

Synonym: CF0762

Alternate gene names: 89898569

Gene position: 905619-907352 (Clockwise)

Preceding gene: 89898565

Following gene: 89898570

Centisome position: 77.65

GC content: 43.08

Gene sequence:

>1734_bases
ATGAGAACTTCCCAGCTTTTTTACAAAACTTCCAAAAATGCGAACAAAGACGCTGCTGTTTTATCCTACGAGTTGCTAGA
AAAAGCAGGTTACATTTTCAAGACAGCAAAGGGTGTTTACACCTATACTCCTCTATTTTGGCGCGTTGCTCTTAAGATGA
TGGACATCGTTCGGGAAGAGCTAAATGCGATTGGGGGACAGGAACTTATGCTACCTATTCTTCATCCTGCAGAACTTTGG
CAAAAAACAGGCCGCTGGGAAGCATTTCGCTCCGAAGGTCTTCTCTACACCTTAACAGATAGAGAAGACAAAGAGTTGTG
TCTTGCTCCCACTCACGAAGAAGTCGTCACTATGTTTGTTTCTCAGTGGTTATCAGGAAGAAAACAACTGCCCATTCATT
TGTATCAAATTGCGACAAAATTCCGAGACGAGATTCGACCAAGATTTGGACTCATGCGTGCTAGGGAATTCCTTATGGAA
GATAGCTATACCTTCTCAGATTCTCCAGAGCAAATGAACGAGCAGTACGATAAACTCAGACGAGCTTATCAAAAGATCTT
CGATAGATTAGAAATTAAGTATGTTATCGTAGAGGCCGACGGAGGAAAGATCGGCAAAGGAAAGTCGGAAGAGTTTCATG
TCCTCTGTTCTCTAGGGGAAGACACAATTTGTGTAAGCGGAGATTACGGAGCTAATATCGAAGCTGCGGTTGCTCTTCCA
GTACAATATACTTATGATAAGGAATTTCTCCCTATAGAAGAAGTCGCCACCCCAGATGTGCGCACAATAGAAAATCTCCA
GGACTTTTTCTCAATCCCTCCTTATAGGATCATGAAAACTCTGGTTGTTAAACTCTCTTACGGAGAAAAAAATACATTCG
TCGCCATAGGCATCCGAGGAGATCGACAGATTAATCTGACGAAAATACGCTCAAAACTCAATGCTGACGAGTGTGCGCTT
GCTTCAGATGAGGAAATACAAAACAATCTAGGAACAGAGAAGGGGTTCGTAGGACCTTTAAACTGTCCTATTGAATTCTA
TGCAGATGAAACCACGCGCTGTATGACAAATTTCATCTGCGCAGGAAACGCCAAAGATAAACACTACAAAAATGTCAACT
GGGATAGGGATATCCCCCGCCCTGAATATGCGGACTTCCTTCTTGCTGAAGCTGGTGACCTCTGTCCATCAAACGGCAAT
GCGCCCTACGAAATTTTTGAAGGTGTGGAAGTTGCCCATATTTTCAATTTAGGCACACGGTATACAGAATGTTTTGATGT
TGGGTTTCAAAATGAACAAGGTGAACAACAAACCTGCTGGATGGGAACCTATGGTATAGGCATAGGCAGAACCCTCGCCG
CGTGTGTAGAACAACTTGCGGATGACCGCGGTATTGTCTGGCCCAAGGCCATCGCTCCTTTCGATATTTCTATTCTCTAC
AATGGCGGTGATTCTGCATCCCAAGAAGCTGCAGAAAAAATCTATACGGAGTTACAAAACTCCGGCTACGCTCCTCTATT
AGATGATCGCAATGAAAGACTTGGGTTTAAATTAAAAGATAGCGATCTTATTGGGATTCCGTATAAACTCATTCTCGGAA
AAACTTTCCTGAACTCAGGAACACTAGAAATAGAATCCCGATCTGGAGAAAAGTTTTCCGTACAACCAAAAGATTTTGTC
CATTGGTGCGAGAATTATCTCCCTCAATCCCAAAAACTCTCCTCCGCCTCTTAG

Upstream 100 bases:

>100_bases
AAGATTTAAAGATTCCTAAAAACCCACATATTTCAGGAAAGAAAAGAGTATCTGTTTCGGTATCTACCGGTTAAAAGTCT
AAGATCTGTGATTGCGCCCC

Downstream 100 bases:

>100_bases
GCCTGGATTAGCATTCTTTAGAGGTGAGTGCTAAACTTCCTTGACCTCGGAGGGTCGTTTTCCTATAATAATCGCCAGAG
AACAGATTATTTGTGTTGGA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MRTSQLFYKTSKNANKDAAVLSYELLEKAGYIFKTAKGVYTYTPLFWRVALKMMDIVREELNAIGGQELMLPILHPAELW
QKTGRWEAFRSEGLLYTLTDREDKELCLAPTHEEVVTMFVSQWLSGRKQLPIHLYQIATKFRDEIRPRFGLMRAREFLME
DSYTFSDSPEQMNEQYDKLRRAYQKIFDRLEIKYVIVEADGGKIGKGKSEEFHVLCSLGEDTICVSGDYGANIEAAVALP
VQYTYDKEFLPIEEVATPDVRTIENLQDFFSIPPYRIMKTLVVKLSYGEKNTFVAIGIRGDRQINLTKIRSKLNADECAL
ASDEEIQNNLGTEKGFVGPLNCPIEFYADETTRCMTNFICAGNAKDKHYKNVNWDRDIPRPEYADFLLAEAGDLCPSNGN
APYEIFEGVEVAHIFNLGTRYTECFDVGFQNEQGEQQTCWMGTYGIGIGRTLAACVEQLADDRGIVWPKAIAPFDISILY
NGGDSASQEAAEKIYTELQNSGYAPLLDDRNERLGFKLKDSDLIGIPYKLILGKTFLNSGTLEIESRSGEKFSVQPKDFV
HWCENYLPQSQKLSSAS

Sequences:

>Translated_577_residues
MRTSQLFYKTSKNANKDAAVLSYELLEKAGYIFKTAKGVYTYTPLFWRVALKMMDIVREELNAIGGQELMLPILHPAELW
QKTGRWEAFRSEGLLYTLTDREDKELCLAPTHEEVVTMFVSQWLSGRKQLPIHLYQIATKFRDEIRPRFGLMRAREFLME
DSYTFSDSPEQMNEQYDKLRRAYQKIFDRLEIKYVIVEADGGKIGKGKSEEFHVLCSLGEDTICVSGDYGANIEAAVALP
VQYTYDKEFLPIEEVATPDVRTIENLQDFFSIPPYRIMKTLVVKLSYGEKNTFVAIGIRGDRQINLTKIRSKLNADECAL
ASDEEIQNNLGTEKGFVGPLNCPIEFYADETTRCMTNFICAGNAKDKHYKNVNWDRDIPRPEYADFLLAEAGDLCPSNGN
APYEIFEGVEVAHIFNLGTRYTECFDVGFQNEQGEQQTCWMGTYGIGIGRTLAACVEQLADDRGIVWPKAIAPFDISILY
NGGDSASQEAAEKIYTELQNSGYAPLLDDRNERLGFKLKDSDLIGIPYKLILGKTFLNSGTLEIESRSGEKFSVQPKDFV
HWCENYLPQSQKLSSAS
>Mature_577_residues
MRTSQLFYKTSKNANKDAAVLSYELLEKAGYIFKTAKGVYTYTPLFWRVALKMMDIVREELNAIGGQELMLPILHPAELW
QKTGRWEAFRSEGLLYTLTDREDKELCLAPTHEEVVTMFVSQWLSGRKQLPIHLYQIATKFRDEIRPRFGLMRAREFLME
DSYTFSDSPEQMNEQYDKLRRAYQKIFDRLEIKYVIVEADGGKIGKGKSEEFHVLCSLGEDTICVSGDYGANIEAAVALP
VQYTYDKEFLPIEEVATPDVRTIENLQDFFSIPPYRIMKTLVVKLSYGEKNTFVAIGIRGDRQINLTKIRSKLNADECAL
ASDEEIQNNLGTEKGFVGPLNCPIEFYADETTRCMTNFICAGNAKDKHYKNVNWDRDIPRPEYADFLLAEAGDLCPSNGN
APYEIFEGVEVAHIFNLGTRYTECFDVGFQNEQGEQQTCWMGTYGIGIGRTLAACVEQLADDRGIVWPKAIAPFDISILY
NGGDSASQEAAEKIYTELQNSGYAPLLDDRNERLGFKLKDSDLIGIPYKLILGKTFLNSGTLEIESRSGEKFSVQPKDFV
HWCENYLPQSQKLSSAS

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=42.9203539823009, Blast_Score=184, Evalue=2e-46,
Organism=Escherichia coli, GI1786392, Length=570, Percent_Identity=43.5087719298246, Blast_Score=457, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI115532348, Length=217, Percent_Identity=38.7096774193548, Blast_Score=156, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6320931, Length=578, Percent_Identity=27.3356401384083, Blast_Score=204, Evalue=3e-53,
Organism=Drosophila melanogaster, GI24656200, Length=220, Percent_Identity=40.4545454545455, Blast_Score=167, Evalue=2e-41,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 65535; Mature: 65535

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSQLFYKTSKNANKDAAVLSYELLEKAGYIFKTAKGVYTYTPLFWRVALKMMDIVREE
CCCCCEEEECCCCCCCCHHHHHHHHHHHCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHH
LNAIGGQELMLPILHPAELWQKTGRWEAFRSEGLLYTLTDREDKELCLAPTHEEVVTMFV
HHHCCCCEEEEECCCHHHHHHHCCCHHHHHCCCCEEEECCCCCCCEEECCCHHHHHHHHH
SQWLSGRKQLPIHLYQIATKFRDEIRPRFGLMRAREFLMEDSYTFSDSPEQMNEQYDKLR
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
RAYQKIFDRLEIKYVIVEADGGKIGKGKSEEFHVLCSLGEDTICVSGDYGANIEAAVALP
HHHHHHHHCCEEEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCEEEEEEEE
VQYTYDKEFLPIEEVATPDVRTIENLQDFFSIPPYRIMKTLVVKLSYGEKNTFVAIGIRG
EEEECCCCCCCHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHEEEECCCCCEEEEEEECC
DRQINLTKIRSKLNADECALASDEEIQNNLGTEKGFVGPLNCPIEFYADETTRCMTNFIC
CCEEEHHHHHHHCCCCHHCCCCCHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHEE
AGNAKDKHYKNVNWDRDIPRPEYADFLLAEAGDLCPSNGNAPYEIFEGVEVAHIFNLGTR
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHCCCEEEHHHHCCCH
YTECFDVGFQNEQGEQQTCWMGTYGIGIGRTLAACVEQLADDRGIVWPKAIAPFDISILY
HHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEE
NGGDSASQEAAEKIYTELQNSGYAPLLDDRNERLGFKLKDSDLIGIPYKLILGKTFLNSG
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCEECCCHHHHHHHHHHCCC
TLEIESRSGEKFSVQPKDFVHWCENYLPQSQKLSSAS
CEEEECCCCCEEECCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRTSQLFYKTSKNANKDAAVLSYELLEKAGYIFKTAKGVYTYTPLFWRVALKMMDIVREE
CCCCCEEEECCCCCCCCHHHHHHHHHHHCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHH
LNAIGGQELMLPILHPAELWQKTGRWEAFRSEGLLYTLTDREDKELCLAPTHEEVVTMFV
HHHCCCCEEEEECCCHHHHHHHCCCHHHHHCCCCEEEECCCCCCCEEECCCHHHHHHHHH
SQWLSGRKQLPIHLYQIATKFRDEIRPRFGLMRAREFLMEDSYTFSDSPEQMNEQYDKLR
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
RAYQKIFDRLEIKYVIVEADGGKIGKGKSEEFHVLCSLGEDTICVSGDYGANIEAAVALP
HHHHHHHHCCEEEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCEEEEEEEE
VQYTYDKEFLPIEEVATPDVRTIENLQDFFSIPPYRIMKTLVVKLSYGEKNTFVAIGIRG
EEEECCCCCCCHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHEEEECCCCCEEEEEEECC
DRQINLTKIRSKLNADECALASDEEIQNNLGTEKGFVGPLNCPIEFYADETTRCMTNFIC
CCEEEHHHHHHHCCCCHHCCCCCHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHEE
AGNAKDKHYKNVNWDRDIPRPEYADFLLAEAGDLCPSNGNAPYEIFEGVEVAHIFNLGTR
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHCCCEEEHHHHCCCH
YTECFDVGFQNEQGEQQTCWMGTYGIGIGRTLAACVEQLADDRGIVWPKAIAPFDISILY
HHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEE
NGGDSASQEAAEKIYTELQNSGYAPLLDDRNERLGFKLKDSDLIGIPYKLILGKTFLNSG
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCEECCCHHHHHHHHHHCCC
TLEIESRSGEKFSVQPKDFVHWCENYLPQSQKLSSAS
CEEEECCCCCEEECCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12682364 [H]