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The map label for this gene is aspC

Identifier: 89898564

GI number: 89898564

Start: 900581

End: 901777

Strand: Direct

Name: aspC

Synonym: CF0757

Alternate gene names: 89898564

Gene position: 900581-901777 (Clockwise)

Preceding gene: 89898562

Following gene: 89898565

Centisome position: 77.22

GC content: 41.35

Gene sequence:

>1197_bases
ATGCGAAGAAACAGCAACTTTTCAAGTTTAGAAACTAACTATCTTTTCTCCAGTATACGTCAAAAAATCCGTTCTTTCCG
AGAAAAACATCCTGAAATCTCCATCATAGACCTATCTATAGGAGACACCACGCAGCCGCTACACGCGTCTGTCATTGACA
CCTTTTCCACATCCGTAAGGAAATTAGGAAATCCCAAAACCTATCGCGGTTACGGTCCTGAATTAGGTCTTCCAGCTTTA
AAAGAAAAACTCTCCGAAGTTTGCTATCGCGGGAAAGTTTCTCCAGAAGAAATTTTTATCTCTGACGGAGCAAAAACAGA
TATTTTTCGTTTATTTTCTCTATTTGGCCCTGGGAAAACCATAGCCGTGCAAGATCCTTCTTATCCGGCCTATATAGATG
CTGCGATTCTTGCAGGAGCTCGTAAGATTGTCAAACTTCCCTGCACTAAAGAAACGGATTTCTTTCCTGTAATTCCTCAA
GAAGAAAACATTGATATTTTCTGTCTATGTTCTCCTAATAACCCCACAGGAACCGTATTAAACAGAGAGCAACTTAAAGA
GCTTATCGATTACGCTAACTCTCATGGGAGCATAATTTTGTTCGATGCTGCCTACAGCGCATTTATCTCCGATCCCTCCT
TACCTACAAGTATCTTCGAAATTCCTGAAGCACGCTTCTGCGCTATAGAAATTAATTCCTTTTCCAAGTCTTTAGGATTT
GCCGGAGTACGTTTGGGATGGAATATTGTTCCTAAAGATCTTAAATATAGTGATGGCTCTCTTATAATTCGTGACTGGGA
ACGCTTCCTATGCACAACATTCAATGGAGCCTCTCTCCCTGTTCAAGAAGCTGCAATTGCTGGAGCTTCGCTTTTCCCTA
ATTTAGAAGCTATCTCAAAGTATCGCTATAACAGCTCGCTTTTGCGTGAGGCCCTACAAAAAGCTGAGTTTGTTGTACAC
GGTGGTGAGCACGCTCCTTACCTTTGGGTTGAAGTTCCCAGCATGATTCCTGATGAAGATTTTTTTGACTTTTTCTTATA
CCAGTATCATATTGCCATCACTCCTGGTAAAGGATTTGGTTCGTGTGGAACGGGATACGTTCGCTTTTCTTCTTTAGGAA
AATCGGAAAATATCGTTGCAGCCTGCCAACGCTTAAGCCAAACGTCTGTATATGATAGAACGGTGTTATCACTATGA

Upstream 100 bases:

>100_bases
AGTTTGGCGTTTGGTTTAGAAAAAAACAGAAAGGTATATAAAAAAAGCGAGACCTTTCTTAAGATGTCTGAACATGAGCT
ACCGATAACGAAAAAACCCA

Downstream 100 bases:

>100_bases
TTCTTCGCAAAATTGCTCAATATGCTTTTTTCCTTTCTCTTATCTGTTCTTTTATTGCTGTGGTTATCTCTTCTCCTAAC
CCCGAACAGGGATCACCGAA

Product: L,L-diaminopimelate aminotransferase

Products: NA

Alternate protein names: DAP-AT; DAP-aminotransferase; LL-DAP-aminotransferase

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MRRNSNFSSLETNYLFSSIRQKIRSFREKHPEISIIDLSIGDTTQPLHASVIDTFSTSVRKLGNPKTYRGYGPELGLPAL
KEKLSEVCYRGKVSPEEIFISDGAKTDIFRLFSLFGPGKTIAVQDPSYPAYIDAAILAGARKIVKLPCTKETDFFPVIPQ
EENIDIFCLCSPNNPTGTVLNREQLKELIDYANSHGSIILFDAAYSAFISDPSLPTSIFEIPEARFCAIEINSFSKSLGF
AGVRLGWNIVPKDLKYSDGSLIIRDWERFLCTTFNGASLPVQEAAIAGASLFPNLEAISKYRYNSSLLREALQKAEFVVH
GGEHAPYLWVEVPSMIPDEDFFDFFLYQYHIAITPGKGFGSCGTGYVRFSSLGKSENIVAACQRLSQTSVYDRTVLSL

Sequences:

>Translated_398_residues
MRRNSNFSSLETNYLFSSIRQKIRSFREKHPEISIIDLSIGDTTQPLHASVIDTFSTSVRKLGNPKTYRGYGPELGLPAL
KEKLSEVCYRGKVSPEEIFISDGAKTDIFRLFSLFGPGKTIAVQDPSYPAYIDAAILAGARKIVKLPCTKETDFFPVIPQ
EENIDIFCLCSPNNPTGTVLNREQLKELIDYANSHGSIILFDAAYSAFISDPSLPTSIFEIPEARFCAIEINSFSKSLGF
AGVRLGWNIVPKDLKYSDGSLIIRDWERFLCTTFNGASLPVQEAAIAGASLFPNLEAISKYRYNSSLLREALQKAEFVVH
GGEHAPYLWVEVPSMIPDEDFFDFFLYQYHIAITPGKGFGSCGTGYVRFSSLGKSENIVAACQRLSQTSVYDRTVLSL
>Mature_398_residues
MRRNSNFSSLETNYLFSSIRQKIRSFREKHPEISIIDLSIGDTTQPLHASVIDTFSTSVRKLGNPKTYRGYGPELGLPAL
KEKLSEVCYRGKVSPEEIFISDGAKTDIFRLFSLFGPGKTIAVQDPSYPAYIDAAILAGARKIVKLPCTKETDFFPVIPQ
EENIDIFCLCSPNNPTGTVLNREQLKELIDYANSHGSIILFDAAYSAFISDPSLPTSIFEIPEARFCAIEINSFSKSLGF
AGVRLGWNIVPKDLKYSDGSLIIRDWERFLCTTFNGASLPVQEAAIAGASLFPNLEAISKYRYNSSLLREALQKAEFVVH
GGEHAPYLWVEVPSMIPDEDFFDFFLYQYHIAITPGKGFGSCGTGYVRFSSLGKSENIVAACQRLSQTSVYDRTVLSL

Specific function: Involved in the synthesis of meso-diaminopimelate (m-DAP or DL-DAP), required for both lysine and peptidoglycan biosynthesis. Catalyzes the direct conversion of tetrahydrodipicolinate to LL-diaminopimelate, a reaction that requires three enzymes in E.coli

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. LL-diaminopimelate aminotransferase subfamily

Homologues:

Organism=Escherichia coli, GI1788722, Length=366, Percent_Identity=25.6830601092896, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1788627, Length=233, Percent_Identity=28.3261802575107, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786816, Length=335, Percent_Identity=23.5820895522388, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPAT_CHLFF (Q253K9)

Other databases:

- EMBL:   AP006861
- RefSeq:   YP_515674.1
- ProteinModelPortal:   Q253K9
- SMR:   Q253K9
- STRING:   Q253K9
- GeneID:   3959238
- GenomeReviews:   AP006861_GR
- KEGG:   cfe:CF0757
- eggNOG:   COG0436
- HOGENOM:   HBG645860
- OMA:   IFEIPEA
- ProtClustDB:   PRK07590
- BioCyc:   CFEL264202:CF0757-MONOMER
- HAMAP:   MF_01642
- InterPro:   IPR004839
- InterPro:   IPR019942
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR03542

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.6.1.83

Molecular weight: Translated: 44267; Mature: 44267

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00105 AA_TRANSFER_CLASS_1

Important sites: BINDING 41-41 BINDING 71-71 BINDING 74-74 BINDING 105-105 BINDING 128-128 BINDING 174-174 BINDING 174-174 BINDING 202-202 BINDING 205-205 BINDING 233-233 BINDING 235-235 BINDING 244-244 BINDING 275-275 BINDING 368-368

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRNSNFSSLETNYLFSSIRQKIRSFREKHPEISIIDLSIGDTTQPLHASVIDTFSTSVR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHH
KLGNPKTYRGYGPELGLPALKEKLSEVCYRGKVSPEEIFISDGAKTDIFRLFSLFGPGKT
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHEEEECCCCHHHHHHHHHHCCCCE
IAVQDPSYPAYIDAAILAGARKIVKLPCTKETDFFPVIPQEENIDIFCLCSPNNPTGTVL
EEEECCCCCHHHHHHHHHCCHHEEECCCCCCCCCEECCCCCCCCEEEEEECCCCCCCCEE
NREQLKELIDYANSHGSIILFDAAYSAFISDPSLPTSIFEIPEARFCAIEINSFSKSLGF
CHHHHHHHHHHHCCCCCEEEEECHHHHHHCCCCCCHHHHHCCCCEEEEEEECCHHHHCCC
AGVRLGWNIVPKDLKYSDGSLIIRDWERFLCTTFNGASLPVQEAAIAGASLFPNLEAISK
CEEEECCEECCCCCEECCCCEEEEEHHHHHEEECCCCCCCHHHHHHCCHHHCCCHHHHHH
YRYNSSLLREALQKAEFVVHGGEHAPYLWVEVPSMIPDEDFFDFFLYQYHIAITPGKGFG
HHCCHHHHHHHHHHCEEEEECCCCCCEEEEECCCCCCCCHHHHEEEEEEEEEEECCCCCC
SCGTGYVRFSSLGKSENIVAACQRLSQTSVYDRTVLSL
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRRNSNFSSLETNYLFSSIRQKIRSFREKHPEISIIDLSIGDTTQPLHASVIDTFSTSVR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHH
KLGNPKTYRGYGPELGLPALKEKLSEVCYRGKVSPEEIFISDGAKTDIFRLFSLFGPGKT
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHEEEECCCCHHHHHHHHHHCCCCE
IAVQDPSYPAYIDAAILAGARKIVKLPCTKETDFFPVIPQEENIDIFCLCSPNNPTGTVL
EEEECCCCCHHHHHHHHHCCHHEEECCCCCCCCCEECCCCCCCCEEEEEECCCCCCCCEE
NREQLKELIDYANSHGSIILFDAAYSAFISDPSLPTSIFEIPEARFCAIEINSFSKSLGF
CHHHHHHHHHHHCCCCCEEEEECHHHHHHCCCCCCHHHHHCCCCEEEEEEECCHHHHCCC
AGVRLGWNIVPKDLKYSDGSLIIRDWERFLCTTFNGASLPVQEAAIAGASLFPNLEAISK
CEEEECCEECCCCCEECCCCEEEEEHHHHHEEECCCCCCCHHHHHHCCHHHCCCHHHHHH
YRYNSSLLREALQKAEFVVHGGEHAPYLWVEVPSMIPDEDFFDFFLYQYHIAITPGKGFG
HHCCHHHHHHHHHHCEEEEECCCCCCEEEEECCCCCCCCHHHHEEEEEEEEEEECCCCCC
SCGTGYVRFSSLGKSENIVAACQRLSQTSVYDRTVLSL
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA