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The map label for this gene is radA [H]

Identifier: 89898472

GI number: 89898472

Start: 768980

End: 770341

Strand: Direct

Name: radA [H]

Synonym: CF0665

Alternate gene names: 89898472

Gene position: 768980-770341 (Clockwise)

Preceding gene: 89898471

Following gene: 89898473

Centisome position: 65.94

GC content: 41.19

Gene sequence:

>1362_bases
ATGACTACAAAGATAAAAACACAGTGGACCTGTAATGAGTGTGGGGCTAGCGCGCCTAAATGGTTAGGCCAATGTCCGGG
ATGCTTACAATGGAACACTCTTATTGAAGAACACATCTCTCCTTCTCAAAATGCAAGAAGGCTGCGACCGCAAGCAACTG
CGGTATCTTTAAATACCGTGGAGCTTCGCGAAGAGGAGCGCTTGTGTATAGGAGAGCCTGGCTGGGATCGCATTTTAGGT
GGTGGAGCTGTTCGTGGAAGTCTTACTCTGTTGGGTGGAGATCCCGGTATCGGTAAATCTACTTTGCTTTTACAAACCTC
GGCAAAGTTTGCCAGTCGAGGTTACAAAGTCCTTTATGTTTGCGGAGAAGAATCTGTAACACAAACATCGCTAAGAGCAC
GTCGTTTACAAATTTCCCACGCCAATATTTATCTTTTTCCCGAAACGAACCTCGATGACATAAAGCAGCAAATTACAACG
CTGCATCCCGATGTTTTAATTATTGATTCTATTCAGATTGTATTTAATCCTACCCTACATTCATCCCCAGGATCTGTGGC
CCAAGTACGAGAAGTTACTTCTGAGCTAATGCACATTGCCAAGCAATCACAAATTACGACTTTTGTTATTGGTCACGTAA
CAAAATCAGGAGAAATTGCTGGCCCCAAAGTTTTAGAACATCTTGTAGACACCGTTCTTTATTTTGAGGGCAATTCGCAT
GCAAACTACCGTATGATACGTTCTGTGAAAAATCGTTTCGGCCCAACGAATGAATTACTTATTTTATCTATGCATACTGA
GGGATTAAAAGAGGTCACTAATCCTTCCGGACTCTTTCTACAAGAAAAGATTACCGAAACCACAGGTTCCACTATCATTC
CTATTATCGAGGGATCTGAAACTCTGCTTGTTGAGATGCAAGCTTTAGCCTCATCTTCTCCTTTTGCCAATCCCATTAGA
AAAACCTCTGGGTTTGATCAAAATCGATTTTTATTACTCTTAGCTGTTCTAGAAAAGCGTGCTCAAATAAAATTACATAC
TTCTGATGTATTTCTTTCTATAGCTGGAGGGTTAAAAATTACAGAACCAGCTGCAGATTTAGGCGCTGGATTAGCCGTAG
TTTCCTCACTATACAATCGTCTCTCTCCTCAAAAATACACATTTACCGGAGAAATAGGCTTGGGAGGGGAAATCCGTCAC
GTCACCCATTTAGAAAGACGTCTTAAAGAAAGCAAACTTATGGGTTTTGAAGGAGCTGTTATTCCTGAGGGACAAATCTT
GGGTTTATCCTATGAAATTAAAGATCTCTTAGATATTCGAGGGGTGAAAACTATAAAAGATGCTATCCGATTGTTACACT
GA

Upstream 100 bases:

>100_bases
CACATTCGTGTGATAGTAAATGATGAGATCTGGGGAGAGGGATTTGCCTTATCGAAAAAAGAAGCCGAGAAACTTGCTGC
TCAAGAAGCTTTAGACGCCA

Downstream 100 bases:

>100_bases
TCCCTTTCTCTCTGATTTTTGCTTAGGCAGACGCCCTCTACGCATAGCTTCTAGAAAATCCACCCTAGCCAAGGCTCAGG
TATATGAATGTGTTCGGCTT

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MTTKIKTQWTCNECGASAPKWLGQCPGCLQWNTLIEEHISPSQNARRLRPQATAVSLNTVELREEERLCIGEPGWDRILG
GGAVRGSLTLLGGDPGIGKSTLLLQTSAKFASRGYKVLYVCGEESVTQTSLRARRLQISHANIYLFPETNLDDIKQQITT
LHPDVLIIDSIQIVFNPTLHSSPGSVAQVREVTSELMHIAKQSQITTFVIGHVTKSGEIAGPKVLEHLVDTVLYFEGNSH
ANYRMIRSVKNRFGPTNELLILSMHTEGLKEVTNPSGLFLQEKITETTGSTIIPIIEGSETLLVEMQALASSSPFANPIR
KTSGFDQNRFLLLLAVLEKRAQIKLHTSDVFLSIAGGLKITEPAADLGAGLAVVSSLYNRLSPQKYTFTGEIGLGGEIRH
VTHLERRLKESKLMGFEGAVIPEGQILGLSYEIKDLLDIRGVKTIKDAIRLLH

Sequences:

>Translated_453_residues
MTTKIKTQWTCNECGASAPKWLGQCPGCLQWNTLIEEHISPSQNARRLRPQATAVSLNTVELREEERLCIGEPGWDRILG
GGAVRGSLTLLGGDPGIGKSTLLLQTSAKFASRGYKVLYVCGEESVTQTSLRARRLQISHANIYLFPETNLDDIKQQITT
LHPDVLIIDSIQIVFNPTLHSSPGSVAQVREVTSELMHIAKQSQITTFVIGHVTKSGEIAGPKVLEHLVDTVLYFEGNSH
ANYRMIRSVKNRFGPTNELLILSMHTEGLKEVTNPSGLFLQEKITETTGSTIIPIIEGSETLLVEMQALASSSPFANPIR
KTSGFDQNRFLLLLAVLEKRAQIKLHTSDVFLSIAGGLKITEPAADLGAGLAVVSSLYNRLSPQKYTFTGEIGLGGEIRH
VTHLERRLKESKLMGFEGAVIPEGQILGLSYEIKDLLDIRGVKTIKDAIRLLH
>Mature_452_residues
TTKIKTQWTCNECGASAPKWLGQCPGCLQWNTLIEEHISPSQNARRLRPQATAVSLNTVELREEERLCIGEPGWDRILGG
GAVRGSLTLLGGDPGIGKSTLLLQTSAKFASRGYKVLYVCGEESVTQTSLRARRLQISHANIYLFPETNLDDIKQQITTL
HPDVLIIDSIQIVFNPTLHSSPGSVAQVREVTSELMHIAKQSQITTFVIGHVTKSGEIAGPKVLEHLVDTVLYFEGNSHA
NYRMIRSVKNRFGPTNELLILSMHTEGLKEVTNPSGLFLQEKITETTGSTIIPIIEGSETLLVEMQALASSSPFANPIRK
TSGFDQNRFLLLLAVLEKRAQIKLHTSDVFLSIAGGLKITEPAADLGAGLAVVSSLYNRLSPQKYTFTGEIGLGGEIRHV
THLERRLKESKLMGFEGAVIPEGQILGLSYEIKDLLDIRGVKTIKDAIRLLH

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=461, Percent_Identity=42.2993492407809, Blast_Score=363, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49719; Mature: 49588

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKIKTQWTCNECGASAPKWLGQCPGCLQWNTLIEEHISPSQNARRLRPQATAVSLNTV
CCCEEEEEECHHHCCCCCCHHHHCCCCCCHHHHHHHHHCCCCCCHHHCCCCCEEEEEEEE
ELREEERLCIGEPGWDRILGGGAVRGSLTLLGGDPGIGKSTLLLQTSAKFASRGYKVLYV
EECCCCCEEECCCCHHHHCCCCEEEEEEEEEECCCCCCCCEEEEEECHHHHHCCCEEEEE
CGEESVTQTSLRARRLQISHANIYLFPETNLDDIKQQITTLHPDVLIIDSIQIVFNPTLH
ECCCCHHHHHHHHHHEEEEECEEEEEECCCHHHHHHHHHHCCCCEEEEEEEEEEECCCCC
SSPGSVAQVREVTSELMHIAKQSQITTFVIGHVTKSGEIAGPKVLEHLVDTVLYFEGNSH
CCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCHHHHHHHHHHEEEEECCCC
ANYRMIRSVKNRFGPTNELLILSMHTEGLKEVTNPSGLFLQEKITETTGSTIIPIIEGSE
CCHHHHHHHHHHCCCCCCEEEEEECCHHHHHHCCCCCCEEHHHHHHCCCCEEEEEEECCC
TLLVEMQALASSSPFANPIRKTSGFDQNRFLLLLAVLEKRAQIKLHTSDVFLSIAGGLKI
EEEEEEHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCEEEEEECEEEEEEECCCEE
TEPAADLGAGLAVVSSLYNRLSPQKYTFTGEIGLGGEIRHVTHLERRLKESKLMGFEGAV
ECCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
IPEGQILGLSYEIKDLLDIRGVKTIKDAIRLLH
CCCCEEEEEEEHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure 
TTKIKTQWTCNECGASAPKWLGQCPGCLQWNTLIEEHISPSQNARRLRPQATAVSLNTV
CCEEEEEECHHHCCCCCCHHHHCCCCCCHHHHHHHHHCCCCCCHHHCCCCCEEEEEEEE
ELREEERLCIGEPGWDRILGGGAVRGSLTLLGGDPGIGKSTLLLQTSAKFASRGYKVLYV
EECCCCCEEECCCCHHHHCCCCEEEEEEEEEECCCCCCCCEEEEEECHHHHHCCCEEEEE
CGEESVTQTSLRARRLQISHANIYLFPETNLDDIKQQITTLHPDVLIIDSIQIVFNPTLH
ECCCCHHHHHHHHHHEEEEECEEEEEECCCHHHHHHHHHHCCCCEEEEEEEEEEECCCCC
SSPGSVAQVREVTSELMHIAKQSQITTFVIGHVTKSGEIAGPKVLEHLVDTVLYFEGNSH
CCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCHHHHHHHHHHEEEEECCCC
ANYRMIRSVKNRFGPTNELLILSMHTEGLKEVTNPSGLFLQEKITETTGSTIIPIIEGSE
CCHHHHHHHHHHCCCCCCEEEEEECCHHHHHHCCCCCCEEHHHHHHCCCCEEEEEEECCC
TLLVEMQALASSSPFANPIRKTSGFDQNRFLLLLAVLEKRAQIKLHTSDVFLSIAGGLKI
EEEEEEHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCEEEEEECEEEEEEECCCEE
TEPAADLGAGLAVVSSLYNRLSPQKYTFTGEIGLGGEIRHVTHLERRLKESKLMGFEGAV
ECCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
IPEGQILGLSYEIKDLLDIRGVKTIKDAIRLLH
CCCCEEEEEEEHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]