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The map label for this gene is folD

Identifier: 89898366

GI number: 89898366

Start: 656960

End: 657823

Strand: Direct

Name: folD

Synonym: CF0559

Alternate gene names: 89898366

Gene position: 656960-657823 (Clockwise)

Preceding gene: 89898365

Following gene: 89898367

Centisome position: 56.33

GC content: 41.32

Gene sequence:

>864_bases
ATGTTATTAAAAGGCACCCCCGTTGCCGAGAGGATTTTAGAAAAACTCAAACAAAAAATCTCTCAAAGTCCAACACCTCC
AGGTCTAGCAGTAGTACTCATTGGGAATGATCCTGCATCCGAAGTTTATGTCGGAATGAAGGTAAAAAAGGCTATGAATC
TAGGAATGGTGTCTAAAGCACATAGACTACCTTCTGATGCAACCCTTACAGACATTCTTAAGCTCATCGAACGATTAAAC
AACGACCCTACAATTCACGGTATCCTAGTTCAGATCCCTCTGCCAAAGCATTTAGATAGTAATGTAATTATTCAAGCAAT
CTCTCCAGAGAAAGATGTGGACGGTCTCCATCCCGTAAATATGGGAAAACTTTTATTGGGACAATTAGGTGGATTTTCTC
CGTGCACTCCGGCTGCGGTTATTGAGCTACTCAACTACTATGATATTCCGCTTCAAGGACGTCATGTTGCTGTTGTGGGA
AGAAGTAATATTGTTGGCAAGCCATTAGCTGCAATGTTGATGCAAAAACACCCCACTACGAATGCAACAGTGACTTTGCT
TCATAGTCAATCACAAAATTTCACGGAAATTTTAAAAACAGCTGATATCATCGTGGCTGCCGTTGGGGTGCCCCTATTTA
TTCAAGAGAATATGATCTCTCCTAATGCTGTCATTGTTGATGTAGGTATATCGCGGGTCGCAACAAATAATGATAAGGGC
TACATGCTAGTCGGAGATGTAGACTTTAATAATGTTGTCACCAAGTGTAAAGCAATCACTCCTGTTCCCGGAGGTGTCGG
ACCTATGACAGTGGCCATGCTAATGAAAAATACATGGGAAAGTTATCAAAAATCCTCCTCATAA

Upstream 100 bases:

>100_bases
CCTTCACAATCGATTTCATTTATAGCGGAATAATTTACCTCCTCCCCTGCATACTCACTCAAGGGATCCAAGAGACCAGA
GGCTATCTAAGGAGTCGCTC

Downstream 100 bases:

>100_bases
CTCTCTTATCCTGGATTCTTCAGGGATGTTCTCCTAAGCTTACAACTTTAGAAGGCGAAAGGATGACCATACCCTACCGC
ATCGTCATAGGAAAACGTCT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MLLKGTPVAERILEKLKQKISQSPTPPGLAVVLIGNDPASEVYVGMKVKKAMNLGMVSKAHRLPSDATLTDILKLIERLN
NDPTIHGILVQIPLPKHLDSNVIIQAISPEKDVDGLHPVNMGKLLLGQLGGFSPCTPAAVIELLNYYDIPLQGRHVAVVG
RSNIVGKPLAAMLMQKHPTTNATVTLLHSQSQNFTEILKTADIIVAAVGVPLFIQENMISPNAVIVDVGISRVATNNDKG
YMLVGDVDFNNVVTKCKAITPVPGGVGPMTVAMLMKNTWESYQKSSS

Sequences:

>Translated_287_residues
MLLKGTPVAERILEKLKQKISQSPTPPGLAVVLIGNDPASEVYVGMKVKKAMNLGMVSKAHRLPSDATLTDILKLIERLN
NDPTIHGILVQIPLPKHLDSNVIIQAISPEKDVDGLHPVNMGKLLLGQLGGFSPCTPAAVIELLNYYDIPLQGRHVAVVG
RSNIVGKPLAAMLMQKHPTTNATVTLLHSQSQNFTEILKTADIIVAAVGVPLFIQENMISPNAVIVDVGISRVATNNDKG
YMLVGDVDFNNVVTKCKAITPVPGGVGPMTVAMLMKNTWESYQKSSS
>Mature_287_residues
MLLKGTPVAERILEKLKQKISQSPTPPGLAVVLIGNDPASEVYVGMKVKKAMNLGMVSKAHRLPSDATLTDILKLIERLN
NDPTIHGILVQIPLPKHLDSNVIIQAISPEKDVDGLHPVNMGKLLLGQLGGFSPCTPAAVIELLNYYDIPLQGRHVAVVG
RSNIVGKPLAAMLMQKHPTTNATVTLLHSQSQNFTEILKTADIIVAAVGVPLFIQENMISPNAVIVDVGISRVATNNDKG
YMLVGDVDFNNVVTKCKAITPVPGGVGPMTVAMLMKNTWESYQKSSS

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=292, Percent_Identity=40.4109589041096, Blast_Score=214, Evalue=7e-56,
Organism=Homo sapiens, GI222418558, Length=294, Percent_Identity=39.7959183673469, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI94721354, Length=288, Percent_Identity=40.9722222222222, Blast_Score=193, Evalue=1e-49,
Organism=Homo sapiens, GI36796743, Length=204, Percent_Identity=28.4313725490196, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1786741, Length=274, Percent_Identity=41.2408759124088, Blast_Score=213, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17568735, Length=298, Percent_Identity=36.9127516778523, Blast_Score=177, Evalue=6e-45,
Organism=Saccharomyces cerevisiae, GI6319558, Length=285, Percent_Identity=42.4561403508772, Blast_Score=222, Evalue=6e-59,
Organism=Saccharomyces cerevisiae, GI6321643, Length=294, Percent_Identity=41.156462585034, Blast_Score=214, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6322933, Length=286, Percent_Identity=28.3216783216783, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24645718, Length=291, Percent_Identity=43.298969072165, Blast_Score=231, Evalue=3e-61,
Organism=Drosophila melanogaster, GI17137370, Length=291, Percent_Identity=43.298969072165, Blast_Score=231, Evalue=3e-61,
Organism=Drosophila melanogaster, GI62472483, Length=291, Percent_Identity=43.298969072165, Blast_Score=231, Evalue=3e-61,
Organism=Drosophila melanogaster, GI45551871, Length=291, Percent_Identity=43.298969072165, Blast_Score=231, Evalue=3e-61,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=40.1360544217687, Blast_Score=206, Evalue=1e-53,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=40.1360544217687, Blast_Score=206, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_CHLFF (Q254F7)

Other databases:

- EMBL:   AP006861
- RefSeq:   YP_515476.1
- ProteinModelPortal:   Q254F7
- SMR:   Q254F7
- STRING:   Q254F7
- GeneID:   3959182
- GenomeReviews:   AP006861_GR
- KEGG:   cfe:CF0559
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   IGKLCLD
- ProtClustDB:   PRK14181
- BioCyc:   CFEL264202:CF0559-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30787; Mature: 30787

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKGTPVAERILEKLKQKISQSPTPPGLAVVLIGNDPASEVYVGMKVKKAMNLGMVSKA
CCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHEEEEEEHHHHHCCCHHHHH
HRLPSDATLTDILKLIERLNNDPTIHGILVQIPLPKHLDSNVIIQAISPEKDVDGLHPVN
HCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCC
MGKLLLGQLGGFSPCTPAAVIELLNYYDIPLQGRHVAVVGRSNIVGKPLAAMLMQKHPTT
HHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCC
NATVTLLHSQSQNFTEILKTADIIVAAVGVPLFIQENMISPNAVIVDVGISRVATNNDKG
CCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCEEEEECCCCC
YMLVGDVDFNNVVTKCKAITPVPGGVGPMTVAMLMKNTWESYQKSSS
EEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLLKGTPVAERILEKLKQKISQSPTPPGLAVVLIGNDPASEVYVGMKVKKAMNLGMVSKA
CCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHEEEEEEHHHHHCCCHHHHH
HRLPSDATLTDILKLIERLNNDPTIHGILVQIPLPKHLDSNVIIQAISPEKDVDGLHPVN
HCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCC
MGKLLLGQLGGFSPCTPAAVIELLNYYDIPLQGRHVAVVGRSNIVGKPLAAMLMQKHPTT
HHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCC
NATVTLLHSQSQNFTEILKTADIIVAAVGVPLFIQENMISPNAVIVDVGISRVATNNDKG
CCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCEEEEECCCCC
YMLVGDVDFNNVVTKCKAITPVPGGVGPMTVAMLMKNTWESYQKSSS
EEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA