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The map label for this gene is oppA3 [H]

Identifier: 89898274

GI number: 89898274

Start: 553841

End: 555433

Strand: Direct

Name: oppA3 [H]

Synonym: CF0467

Alternate gene names: 89898274

Gene position: 553841-555433 (Clockwise)

Preceding gene: 89898273

Following gene: 89898275

Centisome position: 47.49

GC content: 37.98

Gene sequence:

>1593_bases
ATGCGCAAGATATCGTTGGGAATCTGTTGCGGACTCCTTATCTCCTCATCCCTAACGATTCATGGATGTAAAAGCCTTCG
AACATCACAAGAACGTATCTCAATAAATATAAATATGAAAGACGATCCGAGGTCTTTTGACCCTCGCGAAGTCCGTTTGC
TTTCTGACATTAACTTGATTAAGCATATTTATGAGGGATTAGTTCAGGAAAATACACAGACAGGAGATCTAGAACCAGCT
CTTGCTGAAAGCTATTTTCTATCCGATGATGGAACAACATATACCTTTCATTTAAAGCAGGCGTACTGGAGTAATGGTGA
TCCCCTAACTGCTGAAGACTTTATTGCCTCTTGGAAACAAGTTGTAAGCCAAGAAGTTTCTGGGATTTATAATTTTGCCT
TTGACCCTATTAAAAATGTTAAGAAAATTCAACAAGGAAAGCTTTCTCTAGAACATGTTGGTTTTCGCTCCGAGGATGAT
AAAACTCTAGTTATTGAGTTAGAATCTCCTACCTCTCACTTCTTAAAACTTTTAGCATTACCGATTTTCTTCCCAGTACA
TAAAGATCAGCGATGTTCGGAACCAAATCTACCAATTGCTAGCAGCGCATTTTATCCTAAAAAAATAAAACAGAAACAAT
GGCTGCGTTTAGAGAAAAATCCCTACTACTACAATCAGGAGCAAGTTAAAACTCAAACAATTACAGTACATTTTATCCCC
GATCCTAATACCGCAGCTCTATTGTTTAATCAAGGAAAGTTGCATTGGCAAGGGCCTCCCTGGGGTGAACGTATTCGCAC
AGAAACCCTATCGCATTTGCAATCTCAAGGGAATCTCCATTCTTTCGAAGTGGCGGGAACCTCTTGGTTAACTTTTAACG
TAAATAAATTTCCTCTTAATCATATCAAGTTAAGAAAGGCACTAGCCTTAGCTTTGGATAAGGAATCTTTGGTTTCTACG
ATATTTTTAGATAGAACTAAGCCTGCTCAACATTTGTTGCCTAATAGTTTACATACTTACCCAAGTTTAGAGGCTCCTTC
TAAAGAACAACGTAAGTATCTAGCTAAACAACTCTTTCAAGAAGCTTTGGAAGAACTTAATATTTCAGCAAAGGATTTGG
AGAGTCATTCCCTCGTTTTTTCTGCGGGTTCTTCAATAAGCGCTTTGCTTGTTCAGATGATTCGCGAACAATGGAAAGAC
TCTCTGGGATTCTCTATTCCTATAGCAGGAAAGGAATTTGCACTTTTGCAAACGGAACTAGCTTCAGGACATTTCTCATT
GGCAATGGGAGGATGGTTCGCAGATTTTTCCGACCCTATGGCGTTTCTAACAATTTTTGCTTACCCCTCGGGAGTTCCTC
CTTATGTATTAAACCATAAGGATTTCATAAAACTTCTAACAACAATTCAAGAAGAAAGGGATCTAAAAAAACGTCGTGAA
TTGGTTTCTCAAGCATCTCTATATCTAGAAACATTCCATATTATTGAGCCCATTTATCACGATGCTTTTCATTTTGCTCT
AAATAAAAAACTGTCGAACTTTAATTTTTCTCCGACTGGAGTTGTAGATTTTCGCTACGTTAAAGCTTCATAA

Upstream 100 bases:

>100_bases
ATTTTCCTTCTTCTAAGTTATGCACGGATAATGCTGCGATGATTGCAGGATTAGGAAGAGAGCTGTTTCTCTCAAGAAAA
ACTACCCAAGGAATTACTCC

Downstream 100 bases:

>100_bases
TCTTTTTCCTAATACAAGTAGATCAAACAGAATTAATGTTCGTAGAATAAAAACTATTTTTATCTGTGTTGTTCCCAAGA
GAAATGACCTGTTTTTGCTT

Product: ABC transporter/periplasmic oligopeptide binding lipoprotein component

Products: NA

Alternate protein names: Stage 0 sporulation protein KA [H]

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MRKISLGICCGLLISSSLTIHGCKSLRTSQERISININMKDDPRSFDPREVRLLSDINLIKHIYEGLVQENTQTGDLEPA
LAESYFLSDDGTTYTFHLKQAYWSNGDPLTAEDFIASWKQVVSQEVSGIYNFAFDPIKNVKKIQQGKLSLEHVGFRSEDD
KTLVIELESPTSHFLKLLALPIFFPVHKDQRCSEPNLPIASSAFYPKKIKQKQWLRLEKNPYYYNQEQVKTQTITVHFIP
DPNTAALLFNQGKLHWQGPPWGERIRTETLSHLQSQGNLHSFEVAGTSWLTFNVNKFPLNHIKLRKALALALDKESLVST
IFLDRTKPAQHLLPNSLHTYPSLEAPSKEQRKYLAKQLFQEALEELNISAKDLESHSLVFSAGSSISALLVQMIREQWKD
SLGFSIPIAGKEFALLQTELASGHFSLAMGGWFADFSDPMAFLTIFAYPSGVPPYVLNHKDFIKLLTTIQEERDLKKRRE
LVSQASLYLETFHIIEPIYHDAFHFALNKKLSNFNFSPTGVVDFRYVKAS

Sequences:

>Translated_530_residues
MRKISLGICCGLLISSSLTIHGCKSLRTSQERISININMKDDPRSFDPREVRLLSDINLIKHIYEGLVQENTQTGDLEPA
LAESYFLSDDGTTYTFHLKQAYWSNGDPLTAEDFIASWKQVVSQEVSGIYNFAFDPIKNVKKIQQGKLSLEHVGFRSEDD
KTLVIELESPTSHFLKLLALPIFFPVHKDQRCSEPNLPIASSAFYPKKIKQKQWLRLEKNPYYYNQEQVKTQTITVHFIP
DPNTAALLFNQGKLHWQGPPWGERIRTETLSHLQSQGNLHSFEVAGTSWLTFNVNKFPLNHIKLRKALALALDKESLVST
IFLDRTKPAQHLLPNSLHTYPSLEAPSKEQRKYLAKQLFQEALEELNISAKDLESHSLVFSAGSSISALLVQMIREQWKD
SLGFSIPIAGKEFALLQTELASGHFSLAMGGWFADFSDPMAFLTIFAYPSGVPPYVLNHKDFIKLLTTIQEERDLKKRRE
LVSQASLYLETFHIIEPIYHDAFHFALNKKLSNFNFSPTGVVDFRYVKAS
>Mature_530_residues
MRKISLGICCGLLISSSLTIHGCKSLRTSQERISININMKDDPRSFDPREVRLLSDINLIKHIYEGLVQENTQTGDLEPA
LAESYFLSDDGTTYTFHLKQAYWSNGDPLTAEDFIASWKQVVSQEVSGIYNFAFDPIKNVKKIQQGKLSLEHVGFRSEDD
KTLVIELESPTSHFLKLLALPIFFPVHKDQRCSEPNLPIASSAFYPKKIKQKQWLRLEKNPYYYNQEQVKTQTITVHFIP
DPNTAALLFNQGKLHWQGPPWGERIRTETLSHLQSQGNLHSFEVAGTSWLTFNVNKFPLNHIKLRKALALALDKESLVST
IFLDRTKPAQHLLPNSLHTYPSLEAPSKEQRKYLAKQLFQEALEELNISAKDLESHSLVFSAGSSISALLVQMIREQWKD
SLGFSIPIAGKEFALLQTELASGHFSLAMGGWFADFSDPMAFLTIFAYPSGVPPYVLNHKDFIKLLTTIQEERDLKKRRE
LVSQASLYLETFHIIEPIYHDAFHFALNKKLSNFNFSPTGVVDFRYVKAS

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, It binds peptides up to five amino acids long with high affinity. Also required for sporulation and competence [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI87081878, Length=523, Percent_Identity=24.8565965583174, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI1787495, Length=512, Percent_Identity=23.828125, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1789397, Length=485, Percent_Identity=25.979381443299, Blast_Score=145, Evalue=8e-36,
Organism=Escherichia coli, GI1789887, Length=493, Percent_Identity=23.1237322515213, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787052, Length=341, Percent_Identity=23.4604105571848, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60352; Mature: 60352

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKISLGICCGLLISSSLTIHGCKSLRTSQERISININMKDDPRSFDPREVRLLSDINLI
CCEEHHHHHHHHHHHCCCEEECHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHH
KHIYEGLVQENTQTGDLEPALAESYFLSDDGTTYTFHLKQAYWSNGDPLTAEDFIASWKQ
HHHHHHHHHCCCCCCCCCHHHHHHEEECCCCCEEEEEEEEHHCCCCCCCCHHHHHHHHHH
VVSQEVSGIYNFAFDPIKNVKKIQQGKLSLEHVGFRSEDDKTLVIELESPTSHFLKLLAL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCEEEEEECCCHHHHHHHHHH
PIFFPVHKDQRCSEPNLPIASSAFYPKKIKQKQWLRLEKNPYYYNQEQVKTQTITVHFIP
HHHEECCCCCCCCCCCCCCCCCCCCHHHHCHHHHEEEECCCCEECHHHEEEEEEEEEEEC
DPNTAALLFNQGKLHWQGPPWGERIRTETLSHLQSQGNLHSFEVAGTSWLTFNVNKFPLN
CCCCEEEEEECCEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCH
HIKLRKALALALDKESLVSTIFLDRTKPAQHLLPNSLHTYPSLEAPSKEQRKYLAKQLFQ
HHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
EALEELNISAKDLESHSLVFSAGSSISALLVQMIREQWKDSLGFSIPIAGKEFALLQTEL
HHHHHCCCCHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHH
ASGHFSLAMGGWFADFSDPMAFLTIFAYPSGVPPYVLNHKDFIKLLTTIQEERDLKKRRE
CCCCEEEEECCCCCCCCCHHHHHHHEECCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHH
LVSQASLYLETFHIIEPIYHDAFHFALNKKLSNFNFSPTGVVDFRYVKAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MRKISLGICCGLLISSSLTIHGCKSLRTSQERISININMKDDPRSFDPREVRLLSDINLI
CCEEHHHHHHHHHHHCCCEEECHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHH
KHIYEGLVQENTQTGDLEPALAESYFLSDDGTTYTFHLKQAYWSNGDPLTAEDFIASWKQ
HHHHHHHHHCCCCCCCCCHHHHHHEEECCCCCEEEEEEEEHHCCCCCCCCHHHHHHHHHH
VVSQEVSGIYNFAFDPIKNVKKIQQGKLSLEHVGFRSEDDKTLVIELESPTSHFLKLLAL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCEEEEEECCCHHHHHHHHHH
PIFFPVHKDQRCSEPNLPIASSAFYPKKIKQKQWLRLEKNPYYYNQEQVKTQTITVHFIP
HHHEECCCCCCCCCCCCCCCCCCCCHHHHCHHHHEEEECCCCEECHHHEEEEEEEEEEEC
DPNTAALLFNQGKLHWQGPPWGERIRTETLSHLQSQGNLHSFEVAGTSWLTFNVNKFPLN
CCCCEEEEEECCEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCH
HIKLRKALALALDKESLVSTIFLDRTKPAQHLLPNSLHTYPSLEAPSKEQRKYLAKQLFQ
HHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
EALEELNISAKDLESHSLVFSAGSSISALLVQMIREQWKDSLGFSIPIAGKEFALLQTEL
HHHHHCCCCHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHH
ASGHFSLAMGGWFADFSDPMAFLTIFAYPSGVPPYVLNHKDFIKLLTTIQEERDLKKRRE
CCCCEEEEECCCCCCCCCHHHHHHHEECCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHH
LVSQASLYLETFHIIEPIYHDAFHFALNKKLSNFNFSPTGVVDFRYVKAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1901616; 1899858; 9384377 [H]