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The map label for this gene is atpB [H]

Identifier: 89898135

GI number: 89898135

Start: 377913

End: 379229

Strand: Direct

Name: atpB [H]

Synonym: CF0328

Alternate gene names: 89898135

Gene position: 377913-379229 (Clockwise)

Preceding gene: 89898134

Following gene: 89898136

Centisome position: 32.4

GC content: 38.42

Gene sequence:

>1317_bases
ATGCAGACAATATATACAAAAATTACCGACATCAAAGGAAACTTAATAACCGTAGAAGCTGAGGGAGCGCGTCTAGGAGA
ATTGGCAGAGATAGAAAGAGTAGACGGAAGGTCTTCTTATGCTTCTGTTTTGCGTTTTGATGCTAAAAAAGTCACTCTGC
AAGTCTTTGGTGGAACTTCAGGGTTGTCCACAGGAGATCGGGTTATATTTCTAGGACGTGCTATGGAGGTTACTTACGGG
GAATCTCTAATAGGTAGAAGGTTAAGTGGTATTGGTAAACCTATTGATGGTGAGGGAGAATGTTTCGGTGATCCGATCGC
AATTTCCACACCAACATTCAATCCTGTTTGCCGTATTGTGCCTAGAGATATGGTGAGAACCAATATCCCAATGATTGATA
TGTTCAATTGTTTGGTGAAATCTCAAAAGATCCCTATTTTCTCTTCTTCAGGAGAGAATCATAACGCCTTATTGATGCGT
ATAGCAGCACAAACAGATGCCGATATTGTAATTATCGGTGGTATGGGATTGACTTTTGTCGATTACAGCTTTTTCGTTGA
AGAGTCTAAAAGGTTAGGATTCTCTGATAAATGCGTAATGTTTATTCACAAAGCTGTTGATGCACCTGTCGAATGTGTGT
TGATTCCTGATATGGCTTTAGCATGTGCTGAAAAATTTGCCGTAGATCACAATAAGAATGTTTTGGTCCTACTTACAGAT
ATGACGGCATTTGCAGATGCTTTAAAAGAAATTGCGATTACCATGGATCAAATTCCTGCAAACCGTGGTTACCCAGGATC
TCTGTATTCCGATCTTGCTTTGCGTTATGAAAAAGCTGTGGATATTGCAAAGGGTGGATCGATTACTTTAATTAGTGTTA
CCACAATGCCTGGAGATGATATTACTCATCCCGTCCCTGATAACACAGGATTTATTACAGAAGGTCAGTTCTACCTTAAA
AATAATCGTATTGATCCATTCGGTTCCTTGTCTCGTTTGAAACAATTGGTCATTGGTAAAGTAACTAGAGAAGATCATGG
GGATTTAGCAAACTCATTGATACGGCTTTATGCGGATTCTAGAAAAGCTGCAGAAAGAATGGCTATGGGTTTTAAGTTGT
CAAATTGGGATAAAAAGTTATTAGCATTTGCAGAGCTTTTCGAAACACGATTAATGAGTTTAGAAGTAAATATTCCTTTA
GAAGAAGCTTTGGATATTGGTTGGAAAATTCTTGCTCAAAGTTTCCATTCTGAAGAAGTAGGAATAAAGGAACAATTGAT
CAATAAGTATTGGCCGAAGTCATGTCTTCACAGATAA

Upstream 100 bases:

>100_bases
CCTTAAATGGCCGAAAGTTTCTTTCTGATGAATACAAGGAAGGGATGGAAGTGGTTCTTAGGTTGATAGAGACGAAGAAG
ATATAAACAGCGTAAAGGTT

Downstream 100 bases:

>100_bases
AGCTAACAAAAAATGCCTACCGTTTAGAAAAGATAAAGCTTGCGCGTCTAGAAACTTATTTGCCGACGTTAAAATTAAAA
ATGGCGTTATTGCAGGTAGA

Product: V-type ATP synthase subunit B

Products: ADP; phosphate; H+

Alternate protein names: V-ATPase subunit B [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MQTIYTKITDIKGNLITVEAEGARLGELAEIERVDGRSSYASVLRFDAKKVTLQVFGGTSGLSTGDRVIFLGRAMEVTYG
ESLIGRRLSGIGKPIDGEGECFGDPIAISTPTFNPVCRIVPRDMVRTNIPMIDMFNCLVKSQKIPIFSSSGENHNALLMR
IAAQTDADIVIIGGMGLTFVDYSFFVEESKRLGFSDKCVMFIHKAVDAPVECVLIPDMALACAEKFAVDHNKNVLVLLTD
MTAFADALKEIAITMDQIPANRGYPGSLYSDLALRYEKAVDIAKGGSITLISVTTMPGDDITHPVPDNTGFITEGQFYLK
NNRIDPFGSLSRLKQLVIGKVTREDHGDLANSLIRLYADSRKAAERMAMGFKLSNWDKKLLAFAELFETRLMSLEVNIPL
EEALDIGWKILAQSFHSEEVGIKEQLINKYWPKSCLHR

Sequences:

>Translated_438_residues
MQTIYTKITDIKGNLITVEAEGARLGELAEIERVDGRSSYASVLRFDAKKVTLQVFGGTSGLSTGDRVIFLGRAMEVTYG
ESLIGRRLSGIGKPIDGEGECFGDPIAISTPTFNPVCRIVPRDMVRTNIPMIDMFNCLVKSQKIPIFSSSGENHNALLMR
IAAQTDADIVIIGGMGLTFVDYSFFVEESKRLGFSDKCVMFIHKAVDAPVECVLIPDMALACAEKFAVDHNKNVLVLLTD
MTAFADALKEIAITMDQIPANRGYPGSLYSDLALRYEKAVDIAKGGSITLISVTTMPGDDITHPVPDNTGFITEGQFYLK
NNRIDPFGSLSRLKQLVIGKVTREDHGDLANSLIRLYADSRKAAERMAMGFKLSNWDKKLLAFAELFETRLMSLEVNIPL
EEALDIGWKILAQSFHSEEVGIKEQLINKYWPKSCLHR
>Mature_438_residues
MQTIYTKITDIKGNLITVEAEGARLGELAEIERVDGRSSYASVLRFDAKKVTLQVFGGTSGLSTGDRVIFLGRAMEVTYG
ESLIGRRLSGIGKPIDGEGECFGDPIAISTPTFNPVCRIVPRDMVRTNIPMIDMFNCLVKSQKIPIFSSSGENHNALLMR
IAAQTDADIVIIGGMGLTFVDYSFFVEESKRLGFSDKCVMFIHKAVDAPVECVLIPDMALACAEKFAVDHNKNVLVLLTD
MTAFADALKEIAITMDQIPANRGYPGSLYSDLALRYEKAVDIAKGGSITLISVTTMPGDDITHPVPDNTGFITEGQFYLK
NNRIDPFGSLSRLKQLVIGKVTREDHGDLANSLIRLYADSRKAAERMAMGFKLSNWDKKLLAFAELFETRLMSLEVNIPL
EEALDIGWKILAQSFHSEEVGIKEQLINKYWPKSCLHR

Specific function: Produces ATP from ADP in the presence of a proton gradient across the membrane. The V-type beta chain is a regulatory subunit [H]

COG id: COG1156

COG function: function code C; Archaeal/vacuolar-type H+-ATPase subunit B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATPase alpha/beta chains family [H]

Homologues:

Organism=Homo sapiens, GI19913428, Length=461, Percent_Identity=34.70715835141, Blast_Score=248, Evalue=7e-66,
Organism=Homo sapiens, GI19913426, Length=461, Percent_Identity=34.70715835141, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI32189394, Length=394, Percent_Identity=24.6192893401015, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1788251, Length=332, Percent_Identity=27.1084337349398, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1790170, Length=347, Percent_Identity=24.4956772334294, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1790172, Length=342, Percent_Identity=23.9766081871345, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17570191, Length=461, Percent_Identity=36.0086767895879, Blast_Score=252, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17510931, Length=459, Percent_Identity=34.8583877995643, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI25144756, Length=343, Percent_Identity=25.0728862973761, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71988080, Length=299, Percent_Identity=25.752508361204, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71988063, Length=299, Percent_Identity=25.752508361204, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6319603, Length=465, Percent_Identity=33.7634408602151, Blast_Score=250, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6322581, Length=372, Percent_Identity=23.6559139784946, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI281361666, Length=466, Percent_Identity=34.3347639484979, Blast_Score=252, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24646341, Length=466, Percent_Identity=34.3347639484979, Blast_Score=252, Evalue=3e-67,
Organism=Drosophila melanogaster, GI17136796, Length=466, Percent_Identity=34.3347639484979, Blast_Score=252, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24638766, Length=401, Percent_Identity=25.1870324189526, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI28574560, Length=358, Percent_Identity=26.2569832402235, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24658560, Length=297, Percent_Identity=25.9259259259259, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR004100
- InterPro:   IPR022879 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF02874 ATP-synt_ab_N [H]

EC number: 3.6.3.14

Molecular weight: Translated: 48378; Mature: 48378

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTIYTKITDIKGNLITVEAEGARLGELAEIERVDGRSSYASVLRFDAKKVTLQVFGGTS
CCCEEEEEEECCCCEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHCCCEEEEEEEECCCC
GLSTGDRVIFLGRAMEVTYGESLIGRRLSGIGKPIDGEGECFGDPIAISTPTFNPVCRIV
CCCCCCEEEEEECEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCHHHHHC
PRDMVRTNIPMIDMFNCLVKSQKIPIFSSSGENHNALLMRIAAQTDADIVIIGGMGLTFV
CHHHHHCCCCHHHHHHHHHHCCCCCEEECCCCCCCEEEEEEEECCCCCEEEECCCCCHHH
DYSFFVEESKRLGFSDKCVMFIHKAVDAPVECVLIPDMALACAEKFAVDHNKNVLVLLTD
HHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCEEEEEEC
MTAFADALKEIAITMDQIPANRGYPGSLYSDLALRYEKAVDIAKGGSITLISVTTMPGDD
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCC
ITHPVPDNTGFITEGQFYLKNNRIDPFGSLSRLKQLVIGKVTREDHGDLANSLIRLYADS
CCCCCCCCCCEEECCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RKAAERMAMGFKLSNWDKKLLAFAELFETRLMSLEVNIPLEEALDIGWKILAQSFHSEEV
HHHHHHHHHCCEECCHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCCCC
GIKEQLINKYWPKSCLHR
CHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MQTIYTKITDIKGNLITVEAEGARLGELAEIERVDGRSSYASVLRFDAKKVTLQVFGGTS
CCCEEEEEEECCCCEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHCCCEEEEEEEECCCC
GLSTGDRVIFLGRAMEVTYGESLIGRRLSGIGKPIDGEGECFGDPIAISTPTFNPVCRIV
CCCCCCEEEEEECEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCHHHHHC
PRDMVRTNIPMIDMFNCLVKSQKIPIFSSSGENHNALLMRIAAQTDADIVIIGGMGLTFV
CHHHHHCCCCHHHHHHHHHHCCCCCEEECCCCCCCEEEEEEEECCCCCEEEECCCCCHHH
DYSFFVEESKRLGFSDKCVMFIHKAVDAPVECVLIPDMALACAEKFAVDHNKNVLVLLTD
HHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCEEEEEEC
MTAFADALKEIAITMDQIPANRGYPGSLYSDLALRYEKAVDIAKGGSITLISVTTMPGDD
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCC
ITHPVPDNTGFITEGQFYLKNNRIDPFGSLSRLKQLVIGKVTREDHGDLANSLIRLYADS
CCCCCCCCCCEEECCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RKAAERMAMGFKLSNWDKKLLAFAELFETRLMSLEVNIPLEEALDIGWKILAQSFHSEEV
HHHHHHHHHCCEECCHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCCCC
GIKEQLINKYWPKSCLHR
CHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Borate; Diphosphate; HCO3- [C]

Metal ions: Co2+; Fe2+; Mn2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O; H+

Specific reaction: ATP + H2O + H+(in) = ADP + phosphate + H+(out)

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: Ca2+; CN-; Efrapeptin; Ethidiumbromide; Guanidines various reactions catalyzed by ATPase, analogs; Oligomycin; Quercetin; Trialkyl tinderivatives; Venturicidin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA