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The map label for this gene is mesJ

Identifier: 89898057

GI number: 89898057

Start: 295958

End: 296911

Strand: Direct

Name: mesJ

Synonym: CF0250

Alternate gene names: 89898057

Gene position: 295958-296911 (Clockwise)

Preceding gene: 89898054

Following gene: 89898058

Centisome position: 25.38

GC content: 36.79

Gene sequence:

>954_bases
ATGTTATCTTGTCTACTCAGAAATGATAAGCGATTAGAAGTTTTTTTTTCTTCCTTAGATATGAAAAAAAGCTACCTACT
TGCTTTATCTGGAGGGAGTGATTCATTATTCCTTTTATATCTTCTTAAATCTCGAGGAGTCTCTTTTATCGCTGTTCACG
TAGATTATGGATGGAGGGAGTCATCATATCGTGAAGCTGAGGAACTTGAACTTAGGTGTCAAGCAGAAGGCGTGCCTATT
ATTGTAGATCATGTTCCCCCTGAATATAGGACTTCAAGAGATCAAGAAAATGCTGCACGGCGTTACCGCTATGCGTTATT
TCATAAAGTGTGTCAGAAAAAAAATCTTTCGGGGATATTTTTAGCTCATCATGCTAATGACCAGGCAGAGACTGTTTTAA
AGCGTGTGTTAGAAGGAGCTCATTTAAGTAATTTGAAGGGAATGAGGGGTGAAGTATATTACGAAGGTATCCCTATTTTA
CGACCCCTATTGCATATTCCTAAGATAGTTTTATCCCGTACTTTAGATGCTGCAAATATTCACTATGTTCATGATATAAC
AAATACCGATGAACGGTATTTACGTGCTAGAATGCGCAATAAGATATTTCCTTGGTTGGAAGAGATTTTTGGTAAGAATA
TCACGCAACCTTTACTGACTTTAGCTCAAGATTCTGAGGAGTTGTCTTGTTATATGAAACAACAAGCACAACCATTTCTT
GAAAAAATACGGAAAGAAAATACAACCTTGTCGCTTGAAATTCCTAAAACATTAATAGAACAAGTTTTTCTTACCAAATG
GGTGTGTAAGGAGTTCTTTTATAGTGCTGGGATTGTTGCTTCAAGGCATTTCTTGCAAACAGTTTATGATCATCTAAACC
GTGGTTTGCCGGCACAAATGCGACTTCGAAATAAAAGAGTCATCGTAAAAGCTGGAGTAGTAATGATAGAATAG

Upstream 100 bases:

>100_bases
ACAGGAGTTCTTCAAGGTTAAGGATAAAGAGAAAATATTTTTTCAAGTCAATAAGCCTTAACGCATTTGTAATGAATTTA
TACGGACATGTATTTAGATT

Downstream 100 bases:

>100_bases
AAAGTATATAATGACGATTCTCATCGTTTGTTTGAAGATGATGAGTAGAAGTTCTTTGTATATGCAGAGAGTCATATTTT
TTTTATTTTATAACAGGGTT

Product: PP-loop superfamily ATPase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MLSCLLRNDKRLEVFFSSLDMKKSYLLALSGGSDSLFLLYLLKSRGVSFIAVHVDYGWRESSYREAEELELRCQAEGVPI
IVDHVPPEYRTSRDQENAARRYRYALFHKVCQKKNLSGIFLAHHANDQAETVLKRVLEGAHLSNLKGMRGEVYYEGIPIL
RPLLHIPKIVLSRTLDAANIHYVHDITNTDERYLRARMRNKIFPWLEEIFGKNITQPLLTLAQDSEELSCYMKQQAQPFL
EKIRKENTTLSLEIPKTLIEQVFLTKWVCKEFFYSAGIVASRHFLQTVYDHLNRGLPAQMRLRNKRVIVKAGVVMIE

Sequences:

>Translated_317_residues
MLSCLLRNDKRLEVFFSSLDMKKSYLLALSGGSDSLFLLYLLKSRGVSFIAVHVDYGWRESSYREAEELELRCQAEGVPI
IVDHVPPEYRTSRDQENAARRYRYALFHKVCQKKNLSGIFLAHHANDQAETVLKRVLEGAHLSNLKGMRGEVYYEGIPIL
RPLLHIPKIVLSRTLDAANIHYVHDITNTDERYLRARMRNKIFPWLEEIFGKNITQPLLTLAQDSEELSCYMKQQAQPFL
EKIRKENTTLSLEIPKTLIEQVFLTKWVCKEFFYSAGIVASRHFLQTVYDHLNRGLPAQMRLRNKRVIVKAGVVMIE
>Mature_317_residues
MLSCLLRNDKRLEVFFSSLDMKKSYLLALSGGSDSLFLLYLLKSRGVSFIAVHVDYGWRESSYREAEELELRCQAEGVPI
IVDHVPPEYRTSRDQENAARRYRYALFHKVCQKKNLSGIFLAHHANDQAETVLKRVLEGAHLSNLKGMRGEVYYEGIPIL
RPLLHIPKIVLSRTLDAANIHYVHDITNTDERYLRARMRNKIFPWLEEIFGKNITQPLLTLAQDSEELSCYMKQQAQPFL
EKIRKENTTLSLEIPKTLIEQVFLTKWVCKEFFYSAGIVASRHFLQTVYDHLNRGLPAQMRLRNKRVIVKAGVVMIE

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=192, Percent_Identity=27.6041666666667, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_CHLFF (Q255L6)

Other databases:

- EMBL:   AP006861
- RefSeq:   YP_515167.1
- ProteinModelPortal:   Q255L6
- SMR:   Q255L6
- STRING:   Q255L6
- GeneID:   3958342
- GenomeReviews:   AP006861_GR
- KEGG:   cfe:CF0250
- eggNOG:   COG0037
- HOGENOM:   HBG413885
- OMA:   AQDSEEL
- ProtClustDB:   CLSK871623
- BioCyc:   CFEL264202:CF0250-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 36800; Mature: 36800

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSCLLRNDKRLEVFFSSLDMKKSYLLALSGGSDSLFLLYLLKSRGVSFIAVHVDYGWRE
CCCEEECCCCHHHHHHHHHCCCCEEEEEEECCCCCEEHHEEHHHCCCEEEEEEEECCCCC
SSYREAEELELRCQAEGVPIIVDHVPPEYRTSRDQENAARRYRYALFHKVCQKKNLSGIF
CCCCCHHHHHHEEECCCCCEEEECCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
LAHHANDQAETVLKRVLEGAHLSNLKGMRGEVYYEGIPILRPLLHIPKIVLSRTLDAANI
EEECCCCHHHHHHHHHHCCCCHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCE
HYVHDITNTDERYLRARMRNKIFPWLEEIFGKNITQPLLTLAQDSEELSCYMKQQAQPFL
EEEECCCCCHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHH
EKIRKENTTLSLEIPKTLIEQVFLTKWVCKEFFYSAGIVASRHFLQTVYDHLNRGLPAQM
HHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHH
RLRNKRVIVKAGVVMIE
HHCCCEEEEEECEEEEC
>Mature Secondary Structure
MLSCLLRNDKRLEVFFSSLDMKKSYLLALSGGSDSLFLLYLLKSRGVSFIAVHVDYGWRE
CCCEEECCCCHHHHHHHHHCCCCEEEEEEECCCCCEEHHEEHHHCCCEEEEEEEECCCCC
SSYREAEELELRCQAEGVPIIVDHVPPEYRTSRDQENAARRYRYALFHKVCQKKNLSGIF
CCCCCHHHHHHEEECCCCCEEEECCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
LAHHANDQAETVLKRVLEGAHLSNLKGMRGEVYYEGIPILRPLLHIPKIVLSRTLDAANI
EEECCCCHHHHHHHHHHCCCCHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCE
HYVHDITNTDERYLRARMRNKIFPWLEEIFGKNITQPLLTLAQDSEELSCYMKQQAQPFL
EEEECCCCCHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHH
EKIRKENTTLSLEIPKTLIEQVFLTKWVCKEFFYSAGIVASRHFLQTVYDHLNRGLPAQM
HHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHH
RLRNKRVIVKAGVVMIE
HHCCCEEEEEECEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA