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The map label for this gene is pheS [H]

Identifier: 89898053

GI number: 89898053

Start: 290320

End: 291345

Strand: Direct

Name: pheS [H]

Synonym: CF0246

Alternate gene names: 89898053

Gene position: 290320-291345 (Clockwise)

Preceding gene: 89898052

Following gene: 89898054

Centisome position: 24.89

GC content: 38.5

Gene sequence:

>1026_bases
ATGACGATTCAAGAGGAACTTGAAGCTACAAAGCAACAATTTTGTATCGAACTAAATCAAGTTAACTCTTCAAAGGATCT
TTTTGACCTAAAGGTTCGTTATTTAGGAAAGAAGGGTCTTTTTCGTTGTTTTGCTGATAAGTTAAGGGAGTGTCCTTTAG
ACCAAAAGGCTCTAATGGGTGCTTCCATAAATGAATGCAAAACATATATCGAGGAGCAAATCCGAGAAAAAAATGATTCT
ATTCTTCTAGCAGAAGAGTCCGCAGAATTTCTCAAAGAAAAAATCGATATTACATTACCAGGAGAATCTCATTGTCCTGG
AGGAAAGCATATCGTTAAAAAAGTTTTAGACGATGTTGTGGATATCTTTATTTGTTTGGGATTTTGTGTTCGCGAAGCCC
CGAATATTGAAAGTGAAGAGAATAATTTTTCTCTTCTGAACTTTGAAGAAGATCATCCTGCTAGACAAATGCATGATACT
TTTTATCTAGACGGTAAAACAGTATTACGCACCCATACGTCTAATGTTCAAGTTAGAGAACTTAGCAAAGGGCAACCTCC
CATAAAGGTTGTTGCCCCCGGCCTCTGTTTCCGCAATGAAGATATTTCAGCGCGCTCGCATGTGATTTTTCATCAGGTAG
AGGCTTTCTATCTTGATCGCGATGTAACACTTTCTGACTTGACAGAGATGTTAACAGAGTTTTACCATACGTTCTTTAAA
AGGAAAATAGAACTGCGTTTACGACATAGTTACTTTCCTTTCGTTGAGCCGGGAATAGAAGTAGACGTTTCTTGTGAATG
TCGAGGATCTGGATGTTCCTTGTGTAAACATACTGGTTGGTTGGAAGTTGCTGGAGCTGGTATGATACACCCTCAAGTTT
TGCGCAACAGCGGTATTGATCCTGAAATCTATACAGGCTATGCTGTTGGCATGGGTATTGAAAGGTTAGCCATGTTGAAA
CATGGAATCTCTGATATCCGTCTTTTCTGTGAAAACGATCTAAGGTTTTTACAGCAATTTTCTTAA

Upstream 100 bases:

>100_bases
AATGTTATCAGAAATGGCTATCCACAACCCACAAGGTTTTGCAGAAGTAGCCAATCAGGCTAAAAAAGCTCTAGAGGCAA
CTGTTTAGGGAATAAATCGC

Downstream 100 bases:

>100_bases
GGAAGAATGGCAGAGCGGTTTAATGCACCTGTCTTGAAAACAGGAGACCTGAAAGGGTCCGGGGGTTCGAATCCCTCTTC
TTCCGTTTCTTTATGACTAT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MTIQEELEATKQQFCIELNQVNSSKDLFDLKVRYLGKKGLFRCFADKLRECPLDQKALMGASINECKTYIEEQIREKNDS
ILLAEESAEFLKEKIDITLPGESHCPGGKHIVKKVLDDVVDIFICLGFCVREAPNIESEENNFSLLNFEEDHPARQMHDT
FYLDGKTVLRTHTSNVQVRELSKGQPPIKVVAPGLCFRNEDISARSHVIFHQVEAFYLDRDVTLSDLTEMLTEFYHTFFK
RKIELRLRHSYFPFVEPGIEVDVSCECRGSGCSLCKHTGWLEVAGAGMIHPQVLRNSGIDPEIYTGYAVGMGIERLAMLK
HGISDIRLFCENDLRFLQQFS

Sequences:

>Translated_341_residues
MTIQEELEATKQQFCIELNQVNSSKDLFDLKVRYLGKKGLFRCFADKLRECPLDQKALMGASINECKTYIEEQIREKNDS
ILLAEESAEFLKEKIDITLPGESHCPGGKHIVKKVLDDVVDIFICLGFCVREAPNIESEENNFSLLNFEEDHPARQMHDT
FYLDGKTVLRTHTSNVQVRELSKGQPPIKVVAPGLCFRNEDISARSHVIFHQVEAFYLDRDVTLSDLTEMLTEFYHTFFK
RKIELRLRHSYFPFVEPGIEVDVSCECRGSGCSLCKHTGWLEVAGAGMIHPQVLRNSGIDPEIYTGYAVGMGIERLAMLK
HGISDIRLFCENDLRFLQQFS
>Mature_340_residues
TIQEELEATKQQFCIELNQVNSSKDLFDLKVRYLGKKGLFRCFADKLRECPLDQKALMGASINECKTYIEEQIREKNDSI
LLAEESAEFLKEKIDITLPGESHCPGGKHIVKKVLDDVVDIFICLGFCVREAPNIESEENNFSLLNFEEDHPARQMHDTF
YLDGKTVLRTHTSNVQVRELSKGQPPIKVVAPGLCFRNEDISARSHVIFHQVEAFYLDRDVTLSDLTEMLTEFYHTFFKR
KIELRLRHSYFPFVEPGIEVDVSCECRGSGCSLCKHTGWLEVAGAGMIHPQVLRNSGIDPEIYTGYAVGMGIERLAMLKH
GISDIRLFCENDLRFLQQFS

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5729820, Length=235, Percent_Identity=31.9148936170213, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI4758340, Length=278, Percent_Identity=30.5755395683453, Blast_Score=102, Evalue=5e-22,
Organism=Escherichia coli, GI1788007, Length=323, Percent_Identity=42.4148606811146, Blast_Score=281, Evalue=4e-77,
Organism=Caenorhabditis elegans, GI32563657, Length=278, Percent_Identity=28.4172661870504, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17508957, Length=278, Percent_Identity=28.4172661870504, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI32566635, Length=249, Percent_Identity=27.710843373494, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321087, Length=272, Percent_Identity=27.5735294117647, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6325304, Length=101, Percent_Identity=35.6435643564356, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI18858079, Length=279, Percent_Identity=29.7491039426523, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17137424, Length=263, Percent_Identity=29.277566539924, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 38994; Mature: 38863

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIQEELEATKQQFCIELNQVNSSKDLFDLKVRYLGKKGLFRCFADKLRECPLDQKALMG
CCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHC
ASINECKTYIEEQIREKNDSILLAEESAEFLKEKIDITLPGESHCPGGKHIVKKVLDDVV
CCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCEECCCCCCCCCHHHHHHHHHHHHH
DIFICLGFCVREAPNIESEENNFSLLNFEEDHPARQMHDTFYLDGKTVLRTHTSNVQVRE
HHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHCCEEEECCCEEEEECCCCCEEEE
LSKGQPPIKVVAPGLCFRNEDISARSHVIFHQVEAFYLDRDVTLSDLTEMLTEFYHTFFK
CCCCCCCEEEEECCCEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
RKIELRLRHSYFPFVEPGIEVDVSCECRGSGCSLCKHTGWLEVAGAGMIHPQVLRNSGID
HHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCHHHCCCCEEECCCCCCCHHHHHCCCCC
PEIYTGYAVGMGIERLAMLKHGISDIRLFCENDLRFLQQFS
CCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIQEELEATKQQFCIELNQVNSSKDLFDLKVRYLGKKGLFRCFADKLRECPLDQKALMG
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHC
ASINECKTYIEEQIREKNDSILLAEESAEFLKEKIDITLPGESHCPGGKHIVKKVLDDVV
CCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCEECCCCCCCCCHHHHHHHHHHHHH
DIFICLGFCVREAPNIESEENNFSLLNFEEDHPARQMHDTFYLDGKTVLRTHTSNVQVRE
HHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHCCEEEECCCEEEEECCCCCEEEE
LSKGQPPIKVVAPGLCFRNEDISARSHVIFHQVEAFYLDRDVTLSDLTEMLTEFYHTFFK
CCCCCCCEEEEECCCEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
RKIELRLRHSYFPFVEPGIEVDVSCECRGSGCSLCKHTGWLEVAGAGMIHPQVLRNSGID
HHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCHHHCCCCEEECCCCCCCHHHHHCCCCC
PEIYTGYAVGMGIERLAMLKHGISDIRLFCENDLRFLQQFS
CCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA