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The map label for this gene is trmB

Identifier: 89898046

GI number: 89898046

Start: 285992

End: 286666

Strand: Direct

Name: trmB

Synonym: CF0239

Alternate gene names: 89898046

Gene position: 285992-286666 (Clockwise)

Preceding gene: 89898045

Following gene: 89898047

Centisome position: 24.52

GC content: 38.81

Gene sequence:

>675_bases
ATGAAACCCCAAGATTTAAAAGTTCCTTTTTTCTGGGAAGAACGTAGTACCAAAATTAAGGACAACGTTCTCTACGTCCC
AGAGCATTATTTCAAGCATCATCAATTTATCATGCCATCTTGGGAAGAGTTTTTTGGAAATGACAATCCTATTTCCTGCG
AGCTCTGCTCAGGAAATGGTGATTGGGTTGTTGCCCAAGCTAAAGAAACTCCTGAGGTGAATTGGATAGCTGTTGAGAAG
CGGTTTGATAGGGTAAGAAAAATCTGGTCAAAAATGCATAATTGTCGGGTTACTAACCTGAGAATTGTTTGCGGTGATGC
GCAAACATTTTTCCTTCACTATCTAGGAGAAGCAGTAGTACAACGTATAATTGTTAATTTCCCCGATCCATGGCCAAAGT
CGCGTCATCGTAAACACCGTTTATTTCAGGATGAGTTCTTAAACAATGTCGTCAGAGTACTAACAGAGCCTGGAATACTT
ATTCTTGCCACAGATGATAAAAACTATCTTCTTCAAGCAATACAAATCATGAGACAATATTTGAGTCCTACTATGGAAGA
CCCCTACTATTGTGAAGTAGAGAATTACGGGAATTCTTGGTTTGAAACGTTGTGGAGATCCAAAGGTCGAGAAATCTTTT
ATACAGAATTTGTAAAGAAGTTGGGATATAGCTGA

Upstream 100 bases:

>100_bases
AAAACTTTTTCACCTTTTTCTCTTGAAAAGAAAATCCTTAGAAAAAACTAAGTGAGTTTTTATAATTTCCTGAGATATAA
GTTCTTATTATTAATTTTAA

Downstream 100 bases:

>100_bases
CTTGAACAGCTGACCTTCACGATGTCAACGTGACGCTCTAACCAACTGAGCTAATACCCCAAACTAAGCTACAATGTTAT
CGAGAAAAGAGTATCTTTGC

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MKPQDLKVPFFWEERSTKIKDNVLYVPEHYFKHHQFIMPSWEEFFGNDNPISCELCSGNGDWVVAQAKETPEVNWIAVEK
RFDRVRKIWSKMHNCRVTNLRIVCGDAQTFFLHYLGEAVVQRIIVNFPDPWPKSRHRKHRLFQDEFLNNVVRVLTEPGIL
ILATDDKNYLLQAIQIMRQYLSPTMEDPYYCEVENYGNSWFETLWRSKGREIFYTEFVKKLGYS

Sequences:

>Translated_224_residues
MKPQDLKVPFFWEERSTKIKDNVLYVPEHYFKHHQFIMPSWEEFFGNDNPISCELCSGNGDWVVAQAKETPEVNWIAVEK
RFDRVRKIWSKMHNCRVTNLRIVCGDAQTFFLHYLGEAVVQRIIVNFPDPWPKSRHRKHRLFQDEFLNNVVRVLTEPGIL
ILATDDKNYLLQAIQIMRQYLSPTMEDPYYCEVENYGNSWFETLWRSKGREIFYTEFVKKLGYS
>Mature_224_residues
MKPQDLKVPFFWEERSTKIKDNVLYVPEHYFKHHQFIMPSWEEFFGNDNPISCELCSGNGDWVVAQAKETPEVNWIAVEK
RFDRVRKIWSKMHNCRVTNLRIVCGDAQTFFLHYLGEAVVQRIIVNFPDPWPKSRHRKHRLFQDEFLNNVVRVLTEPGIL
ILATDDKNYLLQAIQIMRQYLSPTMEDPYYCEVENYGNSWFETLWRSKGREIFYTEFVKKLGYS

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=140, Percent_Identity=30, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_CHLFF (Q255M7)

Other databases:

- EMBL:   AP006861
- RefSeq:   YP_515156.1
- ProteinModelPortal:   Q255M7
- SMR:   Q255M7
- STRING:   Q255M7
- GeneID:   3958670
- GenomeReviews:   AP006861_GR
- KEGG:   cfe:CF0239
- eggNOG:   COG0220
- HOGENOM:   HBG413880
- OMA:   NLRIVCG
- ProtClustDB:   PRK00121
- BioCyc:   CFEL264202:CF0239-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 26838; Mature: 26838

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: NA

Important sites: BINDING 54-54 BINDING 79-79 BINDING 106-106 BINDING 129-129 BINDING 133-133 BINDING 165-165

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPQDLKVPFFWEERSTKIKDNVLYVPEHYFKHHQFIMPSWEEFFGNDNPISCELCSGNG
CCCCCCCCCEEECCCCCCCCCCEEECCHHHHCCCCCCCCCHHHHCCCCCCEEEEEECCCC
DWVVAQAKETPEVNWIAVEKRFDRVRKIWSKMHNCRVTNLRIVCGDAQTFFLHYLGEAVV
CEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHCCEEEEEEEEECCHHHHHHHHHHHHHH
QRIIVNFPDPWPKSRHRKHRLFQDEFLNNVVRVLTEPGILILATDDKNYLLQAIQIMRQY
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHH
LSPTMEDPYYCEVENYGNSWFETLWRSKGREIFYTEFVKKLGYS
HCCCCCCCEEEEEECCCCHHHHHHHHHCCCEEHHHHHHHHHCCC
>Mature Secondary Structure
MKPQDLKVPFFWEERSTKIKDNVLYVPEHYFKHHQFIMPSWEEFFGNDNPISCELCSGNG
CCCCCCCCCEEECCCCCCCCCCEEECCHHHHCCCCCCCCCHHHHCCCCCCEEEEEECCCC
DWVVAQAKETPEVNWIAVEKRFDRVRKIWSKMHNCRVTNLRIVCGDAQTFFLHYLGEAVV
CEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHCCEEEEEEEEECCHHHHHHHHHHHHHH
QRIIVNFPDPWPKSRHRKHRLFQDEFLNNVVRVLTEPGILILATDDKNYLLQAIQIMRQY
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHH
LSPTMEDPYYCEVENYGNSWFETLWRSKGREIFYTEFVKKLGYS
HCCCCCCCEEEEEECCCCHHHHHHHHHCCCEEHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA