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The map label for this gene is nrdB [H]

Identifier: 89898045

GI number: 89898045

Start: 284771

End: 285820

Strand: Direct

Name: nrdB [H]

Synonym: CF0238

Alternate gene names: 89898045

Gene position: 284771-285820 (Clockwise)

Preceding gene: 89898044

Following gene: 89898046

Centisome position: 24.42

GC content: 36.48

Gene sequence:

>1050_bases
ATGGAAAGCATGGAAGCAGATATTTTAGAAGGTAAATCAAAACGCGTACAATTAAATAGCAAACGTTTGGTTAATTGTAA
TCAGGTGGACGTGAATCAGCTAGTCCCCATTAAGTATAAATGGGCTTGGGAACATTATTTAAATGGTTGTGCAAATAACT
GGTTGCCAACAGAAGTCCCAATGGCTCGCGATATTGACTTATGGAAATCTAATGAACTTTCCGAAGATGAACGTCGAGTG
ATTCTTTTAAACTTAGGATTTTTCAGTACAGCAGAGAGTTTAGTAGGGAATAACATTGTATTGGCTATTTTTAAGCACAT
TACAAATCCTGAAGTACGTCAATATTTATTGAGACAAGCTTTCGAAGAAGCTGTGCATACTCATACTTTTCTTTATATCT
GTGAGTCTTTAGGACTAGATGAATCCGAAGTTTTCAATGCTTATAACGAGCGCGCGACTATCAAGGCTAAGGATGATTTC
CAAATGGATTTGACCAGGGATGTTTTGGACCCTAATTTCTCTACACATACTATAGAGAATTTGCGCCAATTTATTAAAAA
CTTAGTGGGCTATTACATCATTATGGAGGGGATTTTCTTCTATAGTGGTTTTGTCATGATCCTTTCTTTCCATCGTCAAA
ATAAGATGACGGGAATCGGGGAACAGTATCAGTACATTTTACGCGATGAAACTATTCATTTGAATTTCGGTATTGATCTT
ATCAACGGTATTAAAGAAGAAAATCCTGAAGTATGGTCTCAACAACTTCAAGACGAAATCGTTGATCTTATCCAGAAAGC
CGTGGATTTAGAAATCGAATATGCTAGAGATTGTTTGCCACGAGGCATTCTAGGACTAAGATCTTCGATGTTCATTGACT
ATGTCCGTCATATTGCTGATCGCCGTTTGGAGAGAATCGGTTTGAAACCTATCTATAATTCTAAAAATCCATTCCCATGG
ATGAGTGAAACTATAGATCTTAATAAAGAGAAAAACTTTTTTGAAACTCGCGTAACAGAATACCAAACCGCAGCGAATTT
AAATTGGTAA

Upstream 100 bases:

>100_bases
AGTCAGCTTCCACAAGTATTGTCGTAGAAAGAACTAGGGAAACACCCGTCTGTTCTCTCGAAGAAGGTTGTGAATCTTGC
CAGTAATATAAAAGAGAGGG

Downstream 100 bases:

>100_bases
TGCTTTTTAAGTTATTTCTATAAGTGATTGACGACTTTCAGAGTTCCTGAATGTCGTTCAATCGTATTTGGAAAACTTTT
TCACCTTTTTCTCTTGAAAA

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MESMEADILEGKSKRVQLNSKRLVNCNQVDVNQLVPIKYKWAWEHYLNGCANNWLPTEVPMARDIDLWKSNELSEDERRV
ILLNLGFFSTAESLVGNNIVLAIFKHITNPEVRQYLLRQAFEEAVHTHTFLYICESLGLDESEVFNAYNERATIKAKDDF
QMDLTRDVLDPNFSTHTIENLRQFIKNLVGYYIIMEGIFFYSGFVMILSFHRQNKMTGIGEQYQYILRDETIHLNFGIDL
INGIKEENPEVWSQQLQDEIVDLIQKAVDLEIEYARDCLPRGILGLRSSMFIDYVRHIADRRLERIGLKPIYNSKNPFPW
MSETIDLNKEKNFFETRVTEYQTAANLNW

Sequences:

>Translated_349_residues
MESMEADILEGKSKRVQLNSKRLVNCNQVDVNQLVPIKYKWAWEHYLNGCANNWLPTEVPMARDIDLWKSNELSEDERRV
ILLNLGFFSTAESLVGNNIVLAIFKHITNPEVRQYLLRQAFEEAVHTHTFLYICESLGLDESEVFNAYNERATIKAKDDF
QMDLTRDVLDPNFSTHTIENLRQFIKNLVGYYIIMEGIFFYSGFVMILSFHRQNKMTGIGEQYQYILRDETIHLNFGIDL
INGIKEENPEVWSQQLQDEIVDLIQKAVDLEIEYARDCLPRGILGLRSSMFIDYVRHIADRRLERIGLKPIYNSKNPFPW
MSETIDLNKEKNFFETRVTEYQTAANLNW
>Mature_349_residues
MESMEADILEGKSKRVQLNSKRLVNCNQVDVNQLVPIKYKWAWEHYLNGCANNWLPTEVPMARDIDLWKSNELSEDERRV
ILLNLGFFSTAESLVGNNIVLAIFKHITNPEVRQYLLRQAFEEAVHTHTFLYICESLGLDESEVFNAYNERATIKAKDDF
QMDLTRDVLDPNFSTHTIENLRQFIKNLVGYYIIMEGIFFYSGFVMILSFHRQNKMTGIGEQYQYILRDETIHLNFGIDL
INGIKEENPEVWSQQLQDEIVDLIQKAVDLEIEYARDCLPRGILGLRSSMFIDYVRHIADRRLERIGLKPIYNSKNPFPW
MSETIDLNKEKNFFETRVTEYQTAANLNW

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI42544136, Length=344, Percent_Identity=26.7441860465116, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI289177074, Length=344, Percent_Identity=26.7441860465116, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI4557845, Length=312, Percent_Identity=26.6025641025641, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI260064013, Length=312, Percent_Identity=26.6025641025641, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI289177076, Length=291, Percent_Identity=27.8350515463918, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1788567, Length=293, Percent_Identity=26.962457337884, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789031, Length=305, Percent_Identity=20.983606557377, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17551968, Length=312, Percent_Identity=28.8461538461538, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI193206379, Length=302, Percent_Identity=26.158940397351, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17567153, Length=223, Percent_Identity=28.6995515695067, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6322434, Length=316, Percent_Identity=29.746835443038, Blast_Score=133, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6321619, Length=353, Percent_Identity=22.3796033994334, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI18079279, Length=312, Percent_Identity=28.2051282051282, Blast_Score=133, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 40968; Mature: 40968

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESMEADILEGKSKRVQLNSKRLVNCNQVDVNQLVPIKYKWAWEHYLNGCANNWLPTEVP
CCCCHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCC
MARDIDLWKSNELSEDERRVILLNLGFFSTAESLVGNNIVLAIFKHITNPEVRQYLLRQA
CCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHH
FEEAVHTHTFLYICESLGLDESEVFNAYNERATIKAKDDFQMDLTRDVLDPNFSTHTIEN
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCCHHHHHH
LRQFIKNLVGYYIIMEGIFFYSGFVMILSFHRQNKMTGIGEQYQYILRDETIHLNFGIDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEEEECHHH
INGIKEENPEVWSQQLQDEIVDLIQKAVDLEIEYARDCLPRGILGLRSSMFIDYVRHIAD
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRLERIGLKPIYNSKNPFPWMSETIDLNKEKNFFETRVTEYQTAANLNW
HHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MESMEADILEGKSKRVQLNSKRLVNCNQVDVNQLVPIKYKWAWEHYLNGCANNWLPTEVP
CCCCHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCC
MARDIDLWKSNELSEDERRVILLNLGFFSTAESLVGNNIVLAIFKHITNPEVRQYLLRQA
CCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHH
FEEAVHTHTFLYICESLGLDESEVFNAYNERATIKAKDDFQMDLTRDVLDPNFSTHTIEN
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCCHHHHHH
LRQFIKNLVGYYIIMEGIFFYSGFVMILSFHRQNKMTGIGEQYQYILRDETIHLNFGIDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEEEECHHH
INGIKEENPEVWSQQLQDEIVDLIQKAVDLEIEYARDCLPRGILGLRSSMFIDYVRHIAD
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRLERIGLKPIYNSKNPFPWMSETIDLNKEKNFFETRVTEYQTAANLNW
HHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]