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The map label for this gene is murF [H]

Identifier: 89897954

GI number: 89897954

Start: 178327

End: 179673

Strand: Direct

Name: murF [H]

Synonym: CF0147

Alternate gene names: 89897954

Gene position: 178327-179673 (Clockwise)

Preceding gene: 89897951

Following gene: 89897955

Centisome position: 15.29

GC content: 40.83

Gene sequence:

>1347_bases
ATGCAATCTATCTTGCTAGAAGATTGGGTGTCGTTAATGTTGTCCGATGTGAAATATCCGAGGTCTGGGAGAAAGATTTC
TGGAGTTGCTATTGATAGTCGCCAAGTGCGGCCCGGAGATTTGTTCTTCGCTCTTTCTGGTCAATGTACAGACGGTCATC
ATTTTCTAAAACAAGCCGCACAAGCCGGAGCCGTGGCTGCAGTGGTCTCTAAAGACTACTGTGGAGATTCTTTTGGTTTG
GATCTTGTCGTTGTTCAGGATACTACAGAAGCTTTGAAAGAGGCAGGAGAAAACCAAGGGCATCTATTTCAAGGCACTAT
TGTTGGCATTACTGGGTCTATAGGAAAGACAACAACAAAGACTTTTGCAAAAACATTCCTTTCTTCAGTGTATAGGGTAT
ATGCTAGCCCGAAAAGCTATAATTCCCAACTTACTGTCCCTCTAAGTTTACTAATGGCAGATGGAGATGAGGATTTTATC
ATTTTAGAAATGGGAGTTTCTGAACCAGGAAATATGAGGGATTTACTTTCTATAGTAGAGCCCGAGATTTCTGTAATTAC
TAACATAGCCGATCAACACGCTATAAATTTCCCCGACCAAGGGGTGCGAGGCATCGCTGAAGAAAAGAGTCATATTTTAC
AAACCAGTCGTATACAGCTTCTCCCTAAAGATTCTCCTTGGTATTCTCACTTCATAAAACAGTCCCCATCTTCCGAAAAG
TTTTCTTTTGCTTTTTACAATGAGACCGCTGACTTTTATTATAAAGCTATTCAAAAGGATAGTGTTATCATAAGTACTCC
TGAAGGAGATGTAGATTTTGCAGTTTCATTCCCCTATAAGCCTGCCTACAGCAATTTATTAATTTCTTTTTCCTTGGCAT
GGCTTCTAGACGTGCCTGTAGATAGAATTATTCATTCTTGTAGTGATTTGCAACTCCCTCCCATGCGTTTTGAGCTAAGC
ATGCGCAATGGTATTCAAGTAATCAATGATGCTTATAATGCCTGCCCCGAAGCTATGATTGCTGCTCTAGATGCCATTCC
TAATCCTTCAGAAGGGGGAAAGGTTATACTTATCCTAGGCCACATGGCAGAATTAGGAAATTATTCTGAGGAAGGTCATG
CCATTGTCGCCAACAAAGCTTTGTCAAAAGCAAACATAATTTTCTTCATCGGAGAAAAGTGGTTGTCTAGTCAGCATCTG
TTAAAAAGTTCTTCTTGTGAGGTGTCTTTCTATCCGTCTGCTCAGAGTGTTGAAGAAATCTTAAAGAGTGTCGTTCAGCA
AGGAGATATTGTCTTGCTGAAGGGATCGAGAGCTTTGGCTTTAGAGTCTCTGTTGAGCTGTTTTTAA

Upstream 100 bases:

>100_bases
AAGACTCTCTATTTTAATCCCAGAACTTGTAAATGGTATGCTTACTTAGACTGTATGATTCTAAGCGCATGTGTTTTAAT
CAGAGAAAAAAGGCCGAGGA

Downstream 100 bases:

>100_bases
TTTTTAATACTTTACTTATGCATTCTGTGTTTTCTTATCTTAATGAATCTTTGATTCTTTTTCTGGTAACTGTAATGGGA
TTTGCTTTTCTCGTTGGAAT

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MQSILLEDWVSLMLSDVKYPRSGRKISGVAIDSRQVRPGDLFFALSGQCTDGHHFLKQAAQAGAVAAVVSKDYCGDSFGL
DLVVVQDTTEALKEAGENQGHLFQGTIVGITGSIGKTTTKTFAKTFLSSVYRVYASPKSYNSQLTVPLSLLMADGDEDFI
ILEMGVSEPGNMRDLLSIVEPEISVITNIADQHAINFPDQGVRGIAEEKSHILQTSRIQLLPKDSPWYSHFIKQSPSSEK
FSFAFYNETADFYYKAIQKDSVIISTPEGDVDFAVSFPYKPAYSNLLISFSLAWLLDVPVDRIIHSCSDLQLPPMRFELS
MRNGIQVINDAYNACPEAMIAALDAIPNPSEGGKVILILGHMAELGNYSEEGHAIVANKALSKANIIFFIGEKWLSSQHL
LKSSSCEVSFYPSAQSVEEILKSVVQQGDIVLLKGSRALALESLLSCF

Sequences:

>Translated_448_residues
MQSILLEDWVSLMLSDVKYPRSGRKISGVAIDSRQVRPGDLFFALSGQCTDGHHFLKQAAQAGAVAAVVSKDYCGDSFGL
DLVVVQDTTEALKEAGENQGHLFQGTIVGITGSIGKTTTKTFAKTFLSSVYRVYASPKSYNSQLTVPLSLLMADGDEDFI
ILEMGVSEPGNMRDLLSIVEPEISVITNIADQHAINFPDQGVRGIAEEKSHILQTSRIQLLPKDSPWYSHFIKQSPSSEK
FSFAFYNETADFYYKAIQKDSVIISTPEGDVDFAVSFPYKPAYSNLLISFSLAWLLDVPVDRIIHSCSDLQLPPMRFELS
MRNGIQVINDAYNACPEAMIAALDAIPNPSEGGKVILILGHMAELGNYSEEGHAIVANKALSKANIIFFIGEKWLSSQHL
LKSSSCEVSFYPSAQSVEEILKSVVQQGDIVLLKGSRALALESLLSCF
>Mature_448_residues
MQSILLEDWVSLMLSDVKYPRSGRKISGVAIDSRQVRPGDLFFALSGQCTDGHHFLKQAAQAGAVAAVVSKDYCGDSFGL
DLVVVQDTTEALKEAGENQGHLFQGTIVGITGSIGKTTTKTFAKTFLSSVYRVYASPKSYNSQLTVPLSLLMADGDEDFI
ILEMGVSEPGNMRDLLSIVEPEISVITNIADQHAINFPDQGVRGIAEEKSHILQTSRIQLLPKDSPWYSHFIKQSPSSEK
FSFAFYNETADFYYKAIQKDSVIISTPEGDVDFAVSFPYKPAYSNLLISFSLAWLLDVPVDRIIHSCSDLQLPPMRFELS
MRNGIQVINDAYNACPEAMIAALDAIPNPSEGGKVILILGHMAELGNYSEEGHAIVANKALSKANIIFFIGEKWLSSQHL
LKSSSCEVSFYPSAQSVEEILKSVVQQGDIVLLKGSRALALESLLSCF

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=434, Percent_Identity=26.4976958525346, Blast_Score=121, Evalue=9e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 49026; Mature: 49026

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSILLEDWVSLMLSDVKYPRSGRKISGVAIDSRQVRPGDLFFALSGQCTDGHHFLKQAA
CCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHH
QAGAVAAVVSKDYCGDSFGLDLVVVQDTTEALKEAGENQGHLFQGTIVGITGSIGKTTTK
HCCCEEEEHHHCCCCCCCCEEEEEECCHHHHHHHCCCCCCCEEEEEEEEEECCCCCHHHH
TFAKTFLSSVYRVYASPKSYNSQLTVPLSLLMADGDEDFIILEMGVSEPGNMRDLLSIVE
HHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHC
PEISVITNIADQHAINFPDQGVRGIAEEKSHILQTSRIQLLPKDSPWYSHFIKQSPSSEK
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHCCCCCCC
FSFAFYNETADFYYKAIQKDSVIISTPEGDVDFAVSFPYKPAYSNLLISFSLAWLLDVPV
EEEEEECCCHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCH
DRIIHSCSDLQLPPMRFELSMRNGIQVINDAYNACPEAMIAALDAIPNPSEGGKVILILG
HHHHHHHCCCCCCCCEEEEEHHCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCCEEEEEEE
HMAELGNYSEEGHAIVANKALSKANIIFFIGEKWLSSQHLLKSSSCEVSFYPSAQSVEEI
CHHHHCCCCCCCCEEEEECCCCCCCEEEEECCHHCCHHHHHCCCCCCEEECCCHHHHHHH
LKSVVQQGDIVLLKGSRALALESLLSCF
HHHHHCCCCEEEEECCCHHHHHHHHHHC
>Mature Secondary Structure
MQSILLEDWVSLMLSDVKYPRSGRKISGVAIDSRQVRPGDLFFALSGQCTDGHHFLKQAA
CCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHH
QAGAVAAVVSKDYCGDSFGLDLVVVQDTTEALKEAGENQGHLFQGTIVGITGSIGKTTTK
HCCCEEEEHHHCCCCCCCCEEEEEECCHHHHHHHCCCCCCCEEEEEEEEEECCCCCHHHH
TFAKTFLSSVYRVYASPKSYNSQLTVPLSLLMADGDEDFIILEMGVSEPGNMRDLLSIVE
HHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHC
PEISVITNIADQHAINFPDQGVRGIAEEKSHILQTSRIQLLPKDSPWYSHFIKQSPSSEK
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHCCCCCCC
FSFAFYNETADFYYKAIQKDSVIISTPEGDVDFAVSFPYKPAYSNLLISFSLAWLLDVPV
EEEEEECCCHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCH
DRIIHSCSDLQLPPMRFELSMRNGIQVINDAYNACPEAMIAALDAIPNPSEGGKVILILG
HHHHHHHCCCCCCCCEEEEEHHCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCCEEEEEEE
HMAELGNYSEEGHAIVANKALSKANIIFFIGEKWLSSQHLLKSSSCEVSFYPSAQSVEEI
CHHHHCCCCCCCCEEEEECCCCCCCEEEEECCHHCCHHHHHCCCCCCEEECCCHHHHHHH
LKSVVQQGDIVLLKGSRALALESLLSCF
HHHHHCCCCEEEEECCCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]