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The map label for this gene is ribH

Identifier: 89897927

GI number: 89897927

Start: 138167

End: 138631

Strand: Direct

Name: ribH

Synonym: CF0120

Alternate gene names: 89897927

Gene position: 138167-138631 (Clockwise)

Preceding gene: 89897926

Following gene: 89897929

Centisome position: 11.85

GC content: 44.52

Gene sequence:

>465_bases
ATGAAAATATTGAAAGGGACGGCATCAGCAAGAGGCATGAGAGTTGCTATTGTTGGCGCTTGTTTTAATGGACCTATAGC
AGACGCATTGGTCTCAGGAGCGCAACAAACTTTTCTGGATCTAGGCGGTTCAGAAGACATGCTGACTGTGGTGCGTGTTC
CAGGATCTTTTGAGATTCCTTGCACGTTAAAAAAGCTTCTCTCCTCAGGAATGGAATATCACGCGATAGTGGCTTGCGGT
GTTCTTATTAAAGGAGAAACTTCACATTATGATCTTATTGCGGATCAAGTGGCGGCACGGGTTAGCGAGCTATCAGTAGA
GTACGGTCTTCCAATTACTTTTTCTGTTATCACAGCTCCGAGTGTAGATTCTGCGTGGCAACGTGCTGGAATCAAAGGAT
CACACTTAGGCGTGTCTGGAATGGAAACAGCTTTAGAAATGGCAAATCTTTTCGAAAAGTTGTAG

Upstream 100 bases:

>100_bases
GGAAAATGAAAATTACCTACGCACAAAAAAAGAGCGTATGGGGCATTGGATAGACCTCTCTTTCGTTAGGGAAGTAAACA
CAAGATAATTCAAGGATTAT

Downstream 100 bases:

>100_bases
TAGAGTAGCGTTGCACTTTATAGTGTAACGCTCACAGAACCATTTAGATGCACACGCACAGCTATATTTTCTGAGAAAAT
ATCTAATCCGCCGCAAGAAC

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MKILKGTASARGMRVAIVGACFNGPIADALVSGAQQTFLDLGGSEDMLTVVRVPGSFEIPCTLKKLLSSGMEYHAIVACG
VLIKGETSHYDLIADQVAARVSELSVEYGLPITFSVITAPSVDSAWQRAGIKGSHLGVSGMETALEMANLFEKL

Sequences:

>Translated_154_residues
MKILKGTASARGMRVAIVGACFNGPIADALVSGAQQTFLDLGGSEDMLTVVRVPGSFEIPCTLKKLLSSGMEYHAIVACG
VLIKGETSHYDLIADQVAARVSELSVEYGLPITFSVITAPSVDSAWQRAGIKGSHLGVSGMETALEMANLFEKL
>Mature_154_residues
MKILKGTASARGMRVAIVGACFNGPIADALVSGAQQTFLDLGGSEDMLTVVRVPGSFEIPCTLKKLLSSGMEYHAIVACG
VLIKGETSHYDLIADQVAARVSELSVEYGLPITFSVITAPSVDSAWQRAGIKGSHLGVSGMETALEMANLFEKL

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=155, Percent_Identity=33.5483870967742, Blast_Score=93, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6324429, Length=149, Percent_Identity=33.5570469798658, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_CHLFF (Q255Z6)

Other databases:

- EMBL:   AP006861
- RefSeq:   YP_515037.1
- ProteinModelPortal:   Q255Z6
- SMR:   Q255Z6
- STRING:   Q255Z6
- GeneID:   3958870
- GenomeReviews:   AP006861_GR
- KEGG:   cfe:CF0120
- eggNOG:   COG0054
- HOGENOM:   HBG311126
- OMA:   KAGNKGW
- ProtClustDB:   PRK00061
- BioCyc:   CFEL264202:CF0120-MONOMER
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16174; Mature: 16174

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILKGTASARGMRVAIVGACFNGPIADALVSGAQQTFLDLGGSEDMLTVVRVPGSFEIP
CCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCC
CTLKKLLSSGMEYHAIVACGVLIKGETSHYDLIADQVAARVSELSVEYGLPITFSVITAP
HHHHHHHHCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
SVDSAWQRAGIKGSHLGVSGMETALEMANLFEKL
CCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKILKGTASARGMRVAIVGACFNGPIADALVSGAQQTFLDLGGSEDMLTVVRVPGSFEIP
CCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCC
CTLKKLLSSGMEYHAIVACGVLIKGETSHYDLIADQVAARVSELSVEYGLPITFSVITAP
HHHHHHHHCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
SVDSAWQRAGIKGSHLGVSGMETALEMANLFEKL
CCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA