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The map label for this gene is rsuB [C]

Identifier: 89897918

GI number: 89897918

Start: 128571

End: 129290

Strand: Direct

Name: rsuB [C]

Synonym: CF0111

Alternate gene names: 89897918

Gene position: 128571-129290 (Clockwise)

Preceding gene: 89897907

Following gene: 89897919

Centisome position: 11.02

GC content: 41.67

Gene sequence:

>720_bases
ATGTCTCGTATGGAGAATATTATGACAAAGGTTCGTCTCAATAAATTTTTGGCTTCTTCGGGAGTTGCGTCTCGTAGAAA
GTGTGATGAAATTATTTTTTCAGGACATGTAACTGTGAACGGTCGCGTAGCTCCGGGTCCTTTCGTTCTTGTGGACGAAG
AAACTGATACCGTGAAGGTCGATGGGCGTCGCGTAGGCATGACCAAGAAGGTATACTTCATGGTGCATAAACCTTTGGGG
TATTTATGTTCTTCGGAGAGAAAATTCCCAGGGGATAAGTTGGTTATCGATTTATTCTCTCATCTTCCTTATCGGGTATT
CACAGTAGGGAGACTAGACAAGGAAACTTCTGGATTAATTTTAGTAACAAATGATGGAGAATTTTCTAATAAGATTATTC
ACCCTTCTTTCGGAATTACCAAAGAGTATTTGCTTAAGGTGAGCCGTGATGTTACTGCGAAAAATCTTGAAGATCTTATG
GGAGGTACGATTATCGATGGTAAAAGAGTCCGCCCTGTTTCTGTTGAGAAAATCCGTCGTGGTACGATTAAAATTATTGT
CAACGAAGGGAAAAAGCACGAGATTCGTTTATTTGCTGAAGCTGCAGGATTGCCTATTCTAGAGCTTTCACGCATTCGTA
TAGGAAGTTTTGTTCTTGGAGGTCTTCGCTACGGGGAATATCGCGAGCTTACCGATGCCGAGATCCTTACCTACATGTAG

Upstream 100 bases:

>100_bases
TAGAAACAAAAAAACTTTGTCTATAGGAAAGATTTTTCTAGATAATATTTTTTTATAACAAAGAACAGTGGACCTCTATT
GGTATTCTTAGGCAAAATAT

Downstream 100 bases:

>100_bases
CCCCTGTTGCTTTTTTATCATTAGTGAGCCATATGTAGCCCTATGGCAGGATTAGTTTTTGTAATCATTTTTAGCGTATT
GTTGGGAGCCTGTCTCGGGG

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MSRMENIMTKVRLNKFLASSGVASRRKCDEIIFSGHVTVNGRVAPGPFVLVDEETDTVKVDGRRVGMTKKVYFMVHKPLG
YLCSSERKFPGDKLVIDLFSHLPYRVFTVGRLDKETSGLILVTNDGEFSNKIIHPSFGITKEYLLKVSRDVTAKNLEDLM
GGTIIDGKRVRPVSVEKIRRGTIKIIVNEGKKHEIRLFAEAAGLPILELSRIRIGSFVLGGLRYGEYRELTDAEILTYM

Sequences:

>Translated_239_residues
MSRMENIMTKVRLNKFLASSGVASRRKCDEIIFSGHVTVNGRVAPGPFVLVDEETDTVKVDGRRVGMTKKVYFMVHKPLG
YLCSSERKFPGDKLVIDLFSHLPYRVFTVGRLDKETSGLILVTNDGEFSNKIIHPSFGITKEYLLKVSRDVTAKNLEDLM
GGTIIDGKRVRPVSVEKIRRGTIKIIVNEGKKHEIRLFAEAAGLPILELSRIRIGSFVLGGLRYGEYRELTDAEILTYM
>Mature_238_residues
SRMENIMTKVRLNKFLASSGVASRRKCDEIIFSGHVTVNGRVAPGPFVLVDEETDTVKVDGRRVGMTKKVYFMVHKPLGY
LCSSERKFPGDKLVIDLFSHLPYRVFTVGRLDKETSGLILVTNDGEFSNKIIHPSFGITKEYLLKVSRDVTAKNLEDLMG
GTIIDGKRVRPVSVEKIRRGTIKIIVNEGKKHEIRLFAEAAGLPILELSRIRIGSFVLGGLRYGEYRELTDAEILTYM

Specific function: Unknown

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=238, Percent_Identity=33.1932773109244, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=31.6239316239316, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI1787524, Length=245, Percent_Identity=31.8367346938775, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI87081838, Length=146, Percent_Identity=28.0821917808219, Blast_Score=67, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 26879; Mature: 26748

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRMENIMTKVRLNKFLASSGVASRRKCDEIIFSGHVTVNGRVAPGPFVLVDEETDTVKV
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEECEEEECCEECCCCEEEEECCCCEEEE
DGRRVGMTKKVYFMVHKPLGYLCSSERKFPGDKLVIDLFSHLPYRVFTVGRLDKETSGLI
CCEEECCEEEEEEEEECCHHHHHCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCEE
LVTNDGEFSNKIIHPSFGITKEYLLKVSRDVTAKNLEDLMGGTIIDGKRVRPVSVEKIRR
EEECCCCCCCEEECCCCCCCHHHHHHHHCCCCHHHHHHHHCCEEECCCCCCCCCHHHHCC
GTIKIIVNEGKKHEIRLFAEAAGLPILELSRIRIGSFVLGGLRYGEYRELTDAEILTYM
CEEEEEEECCCCCEEEEEEECCCCCEEEEHHEEECCEEECCCCCCCCCCCCCCCCEECC
>Mature Secondary Structure 
SRMENIMTKVRLNKFLASSGVASRRKCDEIIFSGHVTVNGRVAPGPFVLVDEETDTVKV
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEECEEEECCEECCCCEEEEECCCCEEEE
DGRRVGMTKKVYFMVHKPLGYLCSSERKFPGDKLVIDLFSHLPYRVFTVGRLDKETSGLI
CCEEECCEEEEEEEEECCHHHHHCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCEE
LVTNDGEFSNKIIHPSFGITKEYLLKVSRDVTAKNLEDLMGGTIIDGKRVRPVSVEKIRR
EEECCCCCCCEEECCCCCCCHHHHHHHHCCCCHHHHHHHHCCEEECCCCCCCCCHHHHCC
GTIKIIVNEGKKHEIRLFAEAAGLPILELSRIRIGSFVLGGLRYGEYRELTDAEILTYM
CEEEEEEECCCCCEEEEEEECCCCCEEEEHHEEECCEEECCCCCCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]