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The map label for this gene is pckA

Identifier: 89897905

GI number: 89897905

Start: 113573

End: 115375

Strand: Direct

Name: pckA

Synonym: CF0098

Alternate gene names: 89897905

Gene position: 113573-115375 (Clockwise)

Preceding gene: 89897904

Following gene: 89897906

Centisome position: 9.74

GC content: 42.76

Gene sequence:

>1803_bases
ATGACCAGTGCATGGAGTAATGAAATTCAGCATGAAGGCTTAAAACAATGGATCCAAGAAGTCGCTGAGTTGGTAACTCC
TGAAGACATCAGAGTTTGTAATGGTTCTGATTCTGAATATGCTGAAGTTTACAGCATTATGCAAAAATCGGGGGTGGCAA
TTCCTTTAAATTCAGATTTACATCCCAATTGTTTCCTTGTGCGTTCTTCTCCTGAAGATGTTGCTCGTGTCGAGAAGTTT
ACCTTTATTTGTACTGCAAAAAAAGAAGAGGCAGGCCCTACGAATAACTGGCGAGATCCTCAAGAGATGCGTCAGGAGTT
ACAAGGTCTTTTCCGTGGTTGTATGCGTGGAAGAACCTTGTATGTAGTTCCTTTTTGTATGGGACCTTTAAATTCTCCTT
TTTCCCTTATTGGTGTTGAGCTTACGGATTCTCCTTATGTTGTTTGTTCTATGAAGATCATGACACGTATGGGAGCAGAG
GTTTTAAAATCCCTAGGAACATCAGGTACTTTCCATAAGTGTTTGCATAGCGTGGGCGTTCCCTTGTCTCCTGGTGAGAA
AGATGTTGCCTGGCCTTGTAATCCCGAGCATATGCGTATAGTCCATTTTCAAGACGATAGTAGTGTGATGTCTTTTGGTA
GTGGTTACGGAGGAAATGCTTTACTTGGAAAGAAATGTGTAGCTTTGCGTTTAGCTTCCTATATAGCACGCTCAAAAAAT
TGGCTTGCTGAGCATATGTTAATTATTGGGGTAACCAATCCTGAAGGACAGAAGAAGTACTTCGCGGCTTCTTTCCCCAG
TGCTTGTGGTAAAACCAATTTGGCTATGTTGAAGCCTAAGATCCCTGGATGGAAGATTGAGTGTATTGGGGACGATATTG
CTTGGATACGCCCGGGCTCTGATGGTAGGTTGTATGCTGTAAATCCTGAATATGGTTTCTTTGGTGTTGCTCCAGGAACA
TCAGAGAAGACAAATCCTAATGCATTAGCAACCTGCAGATCTAATTCGATATTTACTAACGTTGCTTTAACTCCGGATGG
GGATGTTTGGTGGGAGGGGCTAACGAGTCAACCTCCTGCAGGTCTTATAGATTGGCGTGGCAATCCTTGGCAACCTGGGG
GGACGCCGGCTGCACATCCGAATTCTAGGTTTACGACACCCTTGCAGCAATGTCCTGTTTTAGACTCGCAATGGAATAGT
TCCGAGGGAGTGCCCTTAGAAGCAATTATTTTTGGTGGTCGCCGTTCTGATACTATTCCCTTGGTTTACGAGGCTTTAAG
TTGGCAACACGGAGTTACTATTGGAGCAAGCATGTCGTCGGCAACGACGGCAGCTATTGTTGGCGAACAGGGTAAGTTAC
GTCATGATCCTTTTGCAATGTTGCCGTTCTGTGGTTACAACATGGCTCTTTATTTTGATCACTGGTTATCTTTTGCTTCT
AATGCAAGTTTAAAATTACCAAAGATCTATGGTGTGAACTGGTTCCGCAAGGATAAGGATGGCAATTTCATATGGCCTGG
GTTCAGTGAAAACTTACGTGTTTTAGAATGGATTTTCCGAAGAACAAATGGTGAAGAGTCCATTGCTAAGAGAACACCTA
TTGGCTACTTACCAGAAGAATCTTCCTTGAACTTGGAAGGATTAAATTTATCTTCGCAAGCTCTTCAGGATTTGTTGGCT
GTGGATGTTCCTGGCTGGCTTAAGGAAGTTGCCGATGTCCGTGAATACTGTAAGATTTTTGGATCTGATCTTCCTCAAAT
CATCAGCGATGAATTATTCAGAATAGAAAGAGAATTAAAATAA

Upstream 100 bases:

>100_bases
CTGTGATTACAGCACCGCATCCTTTACTTGCTGTTTGCTTGGGCACGGGCAAAGCATTAGAACATTTAGATCAATTCAAA
AAACGCAAGGGGAATACGGT

Downstream 100 bases:

>100_bases
TTAAATAATCAAAGTCACTTGTTATTTATGTTTTAGTTTTGGGGTTAATTATTTTTTATTTTTGAAATATTATTTTTGTT
TTAAAATTAGAAAAATAATT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 600; Mature: 599

Protein sequence:

>600_residues
MTSAWSNEIQHEGLKQWIQEVAELVTPEDIRVCNGSDSEYAEVYSIMQKSGVAIPLNSDLHPNCFLVRSSPEDVARVEKF
TFICTAKKEEAGPTNNWRDPQEMRQELQGLFRGCMRGRTLYVVPFCMGPLNSPFSLIGVELTDSPYVVCSMKIMTRMGAE
VLKSLGTSGTFHKCLHSVGVPLSPGEKDVAWPCNPEHMRIVHFQDDSSVMSFGSGYGGNALLGKKCVALRLASYIARSKN
WLAEHMLIIGVTNPEGQKKYFAASFPSACGKTNLAMLKPKIPGWKIECIGDDIAWIRPGSDGRLYAVNPEYGFFGVAPGT
SEKTNPNALATCRSNSIFTNVALTPDGDVWWEGLTSQPPAGLIDWRGNPWQPGGTPAAHPNSRFTTPLQQCPVLDSQWNS
SEGVPLEAIIFGGRRSDTIPLVYEALSWQHGVTIGASMSSATTAAIVGEQGKLRHDPFAMLPFCGYNMALYFDHWLSFAS
NASLKLPKIYGVNWFRKDKDGNFIWPGFSENLRVLEWIFRRTNGEESIAKRTPIGYLPEESSLNLEGLNLSSQALQDLLA
VDVPGWLKEVADVREYCKIFGSDLPQIISDELFRIERELK

Sequences:

>Translated_600_residues
MTSAWSNEIQHEGLKQWIQEVAELVTPEDIRVCNGSDSEYAEVYSIMQKSGVAIPLNSDLHPNCFLVRSSPEDVARVEKF
TFICTAKKEEAGPTNNWRDPQEMRQELQGLFRGCMRGRTLYVVPFCMGPLNSPFSLIGVELTDSPYVVCSMKIMTRMGAE
VLKSLGTSGTFHKCLHSVGVPLSPGEKDVAWPCNPEHMRIVHFQDDSSVMSFGSGYGGNALLGKKCVALRLASYIARSKN
WLAEHMLIIGVTNPEGQKKYFAASFPSACGKTNLAMLKPKIPGWKIECIGDDIAWIRPGSDGRLYAVNPEYGFFGVAPGT
SEKTNPNALATCRSNSIFTNVALTPDGDVWWEGLTSQPPAGLIDWRGNPWQPGGTPAAHPNSRFTTPLQQCPVLDSQWNS
SEGVPLEAIIFGGRRSDTIPLVYEALSWQHGVTIGASMSSATTAAIVGEQGKLRHDPFAMLPFCGYNMALYFDHWLSFAS
NASLKLPKIYGVNWFRKDKDGNFIWPGFSENLRVLEWIFRRTNGEESIAKRTPIGYLPEESSLNLEGLNLSSQALQDLLA
VDVPGWLKEVADVREYCKIFGSDLPQIISDELFRIERELK
>Mature_599_residues
TSAWSNEIQHEGLKQWIQEVAELVTPEDIRVCNGSDSEYAEVYSIMQKSGVAIPLNSDLHPNCFLVRSSPEDVARVEKFT
FICTAKKEEAGPTNNWRDPQEMRQELQGLFRGCMRGRTLYVVPFCMGPLNSPFSLIGVELTDSPYVVCSMKIMTRMGAEV
LKSLGTSGTFHKCLHSVGVPLSPGEKDVAWPCNPEHMRIVHFQDDSSVMSFGSGYGGNALLGKKCVALRLASYIARSKNW
LAEHMLIIGVTNPEGQKKYFAASFPSACGKTNLAMLKPKIPGWKIECIGDDIAWIRPGSDGRLYAVNPEYGFFGVAPGTS
EKTNPNALATCRSNSIFTNVALTPDGDVWWEGLTSQPPAGLIDWRGNPWQPGGTPAAHPNSRFTTPLQQCPVLDSQWNSS
EGVPLEAIIFGGRRSDTIPLVYEALSWQHGVTIGASMSSATTAAIVGEQGKLRHDPFAMLPFCGYNMALYFDHWLSFASN
ASLKLPKIYGVNWFRKDKDGNFIWPGFSENLRVLEWIFRRTNGEESIAKRTPIGYLPEESSLNLEGLNLSSQALQDLLAV
DVPGWLKEVADVREYCKIFGSDLPQIISDELFRIERELK

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family

Homologues:

Organism=Homo sapiens, GI187281517, Length=602, Percent_Identity=52.1594684385382, Blast_Score=637, Evalue=0.0,
Organism=Homo sapiens, GI66346721, Length=601, Percent_Identity=51.7470881863561, Blast_Score=633, Evalue=0.0,
Organism=Homo sapiens, GI66346723, Length=373, Percent_Identity=53.887399463807, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17555492, Length=611, Percent_Identity=48.2815057283142, Blast_Score=590, Evalue=1e-169,
Organism=Caenorhabditis elegans, GI71989645, Length=602, Percent_Identity=48.1727574750831, Blast_Score=576, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI71989658, Length=602, Percent_Identity=48.1727574750831, Blast_Score=575, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI71989653, Length=591, Percent_Identity=48.2233502538071, Blast_Score=565, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI32564411, Length=540, Percent_Identity=50, Blast_Score=548, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI32564407, Length=538, Percent_Identity=50, Blast_Score=546, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI71989649, Length=501, Percent_Identity=48.502994011976, Blast_Score=481, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI32564409, Length=366, Percent_Identity=50.5464480874317, Blast_Score=380, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17540956, Length=493, Percent_Identity=26.1663286004057, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24655082, Length=607, Percent_Identity=51.2355848434926, Blast_Score=607, Evalue=1e-174,
Organism=Drosophila melanogaster, GI24655085, Length=593, Percent_Identity=50.4215851602024, Blast_Score=606, Evalue=1e-173,
Organism=Drosophila melanogaster, GI161077226, Length=568, Percent_Identity=52.8169014084507, Blast_Score=582, Evalue=1e-166,
Organism=Drosophila melanogaster, GI24655088, Length=494, Percent_Identity=53.6437246963563, Blast_Score=542, Evalue=1e-154,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PCKG_CHLFF (Q256B8)

Other databases:

- EMBL:   AP006861
- RefSeq:   YP_515015.1
- ProteinModelPortal:   Q256B8
- SMR:   Q256B8
- STRING:   Q256B8
- GeneID:   3959148
- GenomeReviews:   AP006861_GR
- KEGG:   cfe:CF0098
- eggNOG:   COG1274
- HOGENOM:   HBG484437
- OMA:   CGYHMGD
- ProtClustDB:   PRK04210
- BioCyc:   CFEL264202:CF0098-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00452
- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PANTHER:   PTHR11561
- PIRSF:   PIRSF001348

Pfam domain/function: PF00821 PEPCK; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.32

Molecular weight: Translated: 66477; Mature: 66346

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00505 PEPCK_GTP

Important sites: ACT_SITE 269-269 BINDING 76-76 BINDING 218-218 BINDING 225-225 BINDING 267-267 BINDING 383-383 BINDING 414-414

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSAWSNEIQHEGLKQWIQEVAELVTPEDIRVCNGSDSEYAEVYSIMQKSGVAIPLNSDL
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCEEEECCCC
HPNCFLVRSSPEDVARVEKFTFICTAKKEEAGPTNNWRDPQEMRQELQGLFRGCMRGRTL
CCCEEEEECCCHHHHHHHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEE
YVVPFCMGPLNSPFSLIGVELTDSPYVVCSMKIMTRMGAEVLKSLGTSGTFHKCLHSVGV
EEEEEECCCCCCCCEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
PLSPGEKDVAWPCNPEHMRIVHFQDDSSVMSFGSGYGGNALLGKKCVALRLASYIARSKN
CCCCCCCCCCCCCCCCCEEEEEEECCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
WLAEHMLIIGVTNPEGQKKYFAASFPSACGKTNLAMLKPKIPGWKIECIGDDIAWIRPGS
HHCCEEEEEEECCCCCCCEEEEECCCCCCCCCCEEEECCCCCCEEEEEECCCEEEEECCC
DGRLYAVNPEYGFFGVAPGTSEKTNPNALATCRSNSIFTNVALTPDGDVWWEGLTSQPPA
CCEEEEECCCCCEEECCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCHHCCCCCCCCCC
GLIDWRGNPWQPGGTPAAHPNSRFTTPLQQCPVLDSQWNSSEGVPLEAIIFGGRRSDTIP
CEEECCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCHH
LVYEALSWQHGVTIGASMSSATTAAIVGEQGKLRHDPFAMLPFCGYNMALYFDHWLSFAS
HHHHHHCCCCCEEEECCCCCCCEEEEECCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHC
NASLKLPKIYGVNWFRKDKDGNFIWPGFSENLRVLEWIFRRTNGEESIAKRTPIGYLPEE
CCCEECCEEECCCEEEECCCCCEECCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCC
SSLNLEGLNLSSQALQDLLAVDVPGWLKEVADVREYCKIFGSDLPQIISDELFRIERELK
CCCCEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSAWSNEIQHEGLKQWIQEVAELVTPEDIRVCNGSDSEYAEVYSIMQKSGVAIPLNSDL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCEEEECCCC
HPNCFLVRSSPEDVARVEKFTFICTAKKEEAGPTNNWRDPQEMRQELQGLFRGCMRGRTL
CCCEEEEECCCHHHHHHHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEE
YVVPFCMGPLNSPFSLIGVELTDSPYVVCSMKIMTRMGAEVLKSLGTSGTFHKCLHSVGV
EEEEEECCCCCCCCEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
PLSPGEKDVAWPCNPEHMRIVHFQDDSSVMSFGSGYGGNALLGKKCVALRLASYIARSKN
CCCCCCCCCCCCCCCCCEEEEEEECCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
WLAEHMLIIGVTNPEGQKKYFAASFPSACGKTNLAMLKPKIPGWKIECIGDDIAWIRPGS
HHCCEEEEEEECCCCCCCEEEEECCCCCCCCCCEEEECCCCCCEEEEEECCCEEEEECCC
DGRLYAVNPEYGFFGVAPGTSEKTNPNALATCRSNSIFTNVALTPDGDVWWEGLTSQPPA
CCEEEEECCCCCEEECCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCHHCCCCCCCCCC
GLIDWRGNPWQPGGTPAAHPNSRFTTPLQQCPVLDSQWNSSEGVPLEAIIFGGRRSDTIP
CEEECCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCHH
LVYEALSWQHGVTIGASMSSATTAAIVGEQGKLRHDPFAMLPFCGYNMALYFDHWLSFAS
HHHHHHCCCCCEEEECCCCCCCEEEEECCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHC
NASLKLPKIYGVNWFRKDKDGNFIWPGFSENLRVLEWIFRRTNGEESIAKRTPIGYLPEE
CCCEECCEEECCCEEEECCCCCEECCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCC
SSLNLEGLNLSSQALQDLLAVDVPGWLKEVADVREYCKIFGSDLPQIISDELFRIERELK
CCCCEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA