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The map label for this gene is bcr [H]

Identifier: 89256994

GI number: 89256994

Start: 1656225

End: 1657406

Strand: Direct

Name: bcr [H]

Synonym: FTL_1725

Alternate gene names: 89256994

Gene position: 1656225-1657406 (Clockwise)

Preceding gene: 89256993

Following gene: 89256995

Centisome position: 87.35

GC content: 34.26

Gene sequence:

>1182_bases
ATGTATAAAGGTATCAGACCATCAATATTTATCTTAATTCTCATGGTCTCTTTGGGACCATTTGGTGATACCATTTATGC
ACCAGCATTGCCTGAGTTAAAAACAATTTTAGGTACTGACTATCCCCATGTGCAGCTTACTATAACCTCATATTTACTTG
GCTACTCGATTAGTCAGCTATTGTATGGACCATTCTCTGATAGATTTGGTCGCAAACCTGTAATGTTGGTAGGAACATGT
TTTTTTATAATTAGCTCTATAATATGTATATTTAGTAACCATATCAATACTTTGATATATGCTAGAATGATACAAGGCTT
TGGTGCAGCTGCTGGTGGAGTTATTGCTACAGTTGCTGTTAAAGATGCTTTCAAAGTTAATGAGCAAGGCGCAGTATTTG
CGATTATGAATATAGCCTTTGCTCTAACTCCAGCATTTGGTGCAGTACTTGGAGTATTCCTTAGTCCTACAGCAATTTTT
TGGGTATTACTAGTTGCAGCTAGTATACTATTCATAAAAGTTGCGATCTTTTTCCCAGAAACAATTAGAGAAAAAAACTT
TGAAGCTTTAACTTTTAAATCTTTCTTTAAGAACTATTTTTTACTTTTTAAAGACCATCAGTTTTTCTTTGCCACTTTTG
TTTTGGGGATAAATATCAGTGTGATATACGCCTGTTTAGTCACAGCCCCAGATATATTTGTAAATATTCTCAAACTCGAG
AAATCTAACTTTTTATACTTATTAACAATAATGGTTACAGCTGTGGTACTAGGGTCTATTGTTTGCTCAAAACTAAGTCG
CCTAATAGCATATAAACATCTTGTAAATTTCGGCATGTTTTGTACTTTGATATCTGGAGTGTTATGCATCTATGCATTCA
AAAAACTTACAGGCTTAGAATTATCAATAGCCTTAACTGCAATATTATCACTAACATTTATAGGTGTCTCATTTAGTGTG
CCTTTACTAACACCTATTGCACTAGAAAACTTTACTACTACTGCAGGAGCTGCCTCGTCTGTGATGGGCTTTATACAAAT
GGGCATAGCCTCAATAACAACTGCAATTATGAGTCAAATCAGCCTAGGCAGTGAATTTACGCTATCTTTTGCATTTGTAA
TTCTACCACTTATTGGTCTAACGATATTTTTACCTTATAGTTGCTATTTCCTCAAGAAATAA

Upstream 100 bases:

>100_bases
CTCCTTTAGCAACAAAAACTACACAAACAACAGAAACTAAATCTAAAGGCATCAATGCTACTATAATAATTGGTGATTTT
AGTAAAGGTAAGCCAAGCTA

Downstream 100 bases:

>100_bases
AGCTTAGTGCTCAGCTAAATTTAGGATTAAAATAATTATATGGTAAAATCTAGATTAACTAATATAATTTTATCGATCTT
AAATTATCAATGCAAAACTA

Product: eflux protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MYKGIRPSIFILILMVSLGPFGDTIYAPALPELKTILGTDYPHVQLTITSYLLGYSISQLLYGPFSDRFGRKPVMLVGTC
FFIISSIICIFSNHINTLIYARMIQGFGAAAGGVIATVAVKDAFKVNEQGAVFAIMNIAFALTPAFGAVLGVFLSPTAIF
WVLLVAASILFIKVAIFFPETIREKNFEALTFKSFFKNYFLLFKDHQFFFATFVLGINISVIYACLVTAPDIFVNILKLE
KSNFLYLLTIMVTAVVLGSIVCSKLSRLIAYKHLVNFGMFCTLISGVLCIYAFKKLTGLELSIALTAILSLTFIGVSFSV
PLLTPIALENFTTTAGAASSVMGFIQMGIASITTAIMSQISLGSEFTLSFAFVILPLIGLTIFLPYSCYFLKK

Sequences:

>Translated_393_residues
MYKGIRPSIFILILMVSLGPFGDTIYAPALPELKTILGTDYPHVQLTITSYLLGYSISQLLYGPFSDRFGRKPVMLVGTC
FFIISSIICIFSNHINTLIYARMIQGFGAAAGGVIATVAVKDAFKVNEQGAVFAIMNIAFALTPAFGAVLGVFLSPTAIF
WVLLVAASILFIKVAIFFPETIREKNFEALTFKSFFKNYFLLFKDHQFFFATFVLGINISVIYACLVTAPDIFVNILKLE
KSNFLYLLTIMVTAVVLGSIVCSKLSRLIAYKHLVNFGMFCTLISGVLCIYAFKKLTGLELSIALTAILSLTFIGVSFSV
PLLTPIALENFTTTAGAASSVMGFIQMGIASITTAIMSQISLGSEFTLSFAFVILPLIGLTIFLPYSCYFLKK
>Mature_393_residues
MYKGIRPSIFILILMVSLGPFGDTIYAPALPELKTILGTDYPHVQLTITSYLLGYSISQLLYGPFSDRFGRKPVMLVGTC
FFIISSIICIFSNHINTLIYARMIQGFGAAAGGVIATVAVKDAFKVNEQGAVFAIMNIAFALTPAFGAVLGVFLSPTAIF
WVLLVAASILFIKVAIFFPETIREKNFEALTFKSFFKNYFLLFKDHQFFFATFVLGINISVIYACLVTAPDIFVNILKLE
KSNFLYLLTIMVTAVVLGSIVCSKLSRLIAYKHLVNFGMFCTLISGVLCIYAFKKLTGLELSIALTAILSLTFIGVSFSV
PLLTPIALENFTTTAGAASSVMGFIQMGIASITTAIMSQISLGSEFTLSFAFVILPLIGLTIFLPYSCYFLKK

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=366, Percent_Identity=27.5956284153005, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787065, Length=315, Percent_Identity=26.031746031746, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI48994889, Length=203, Percent_Identity=30.5418719211823, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87082312, Length=353, Percent_Identity=26.3456090651558, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790146, Length=364, Percent_Identity=24.7252747252747, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790794, Length=221, Percent_Identity=24.4343891402715, Blast_Score=73, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324264, Length=166, Percent_Identity=30.7228915662651, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42978; Mature: 42978

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKGIRPSIFILILMVSLGPFGDTIYAPALPELKTILGTDYPHVQLTITSYLLGYSISQL
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEHHHHHHHHHHHHH
LYGPFSDRFGRKPVMLVGTCFFIISSIICIFSNHINTLIYARMIQGFGAAAGGVIATVAV
HCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
KDAFKVNEQGAVFAIMNIAFALTPAFGAVLGVFLSPTAIFWVLLVAASILFIKVAIFFPE
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
TIREKNFEALTFKSFFKNYFLLFKDHQFFFATFVLGINISVIYACLVTAPDIFVNILKLE
HHHHCCCHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCC
KSNFLYLLTIMVTAVVLGSIVCSKLSRLIAYKHLVNFGMFCTLISGVLCIYAFKKLTGLE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
LSIALTAILSLTFIGVSFSVPLLTPIALENFTTTAGAASSVMGFIQMGIASITTAIMSQI
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SLGSEFTLSFAFVILPLIGLTIFLPYSCYFLKK
CCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECC
>Mature Secondary Structure
MYKGIRPSIFILILMVSLGPFGDTIYAPALPELKTILGTDYPHVQLTITSYLLGYSISQL
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEHHHHHHHHHHHHH
LYGPFSDRFGRKPVMLVGTCFFIISSIICIFSNHINTLIYARMIQGFGAAAGGVIATVAV
HCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
KDAFKVNEQGAVFAIMNIAFALTPAFGAVLGVFLSPTAIFWVLLVAASILFIKVAIFFPE
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
TIREKNFEALTFKSFFKNYFLLFKDHQFFFATFVLGINISVIYACLVTAPDIFVNILKLE
HHHHCCCHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCC
KSNFLYLLTIMVTAVVLGSIVCSKLSRLIAYKHLVNFGMFCTLISGVLCIYAFKKLTGLE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
LSIALTAILSLTFIGVSFSVPLLTPIALENFTTTAGAASSVMGFIQMGIASITTAIMSQI
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SLGSEFTLSFAFVILPLIGLTIFLPYSCYFLKK
CCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]