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The map label for this gene is ansA [H]

Identifier: 89256877

GI number: 89256877

Start: 1529789

End: 1530709

Strand: Reverse

Name: ansA [H]

Synonym: FTL_1600

Alternate gene names: 89256877

Gene position: 1530709-1529789 (Counterclockwise)

Preceding gene: 89256878

Following gene: 89256876

Centisome position: 80.73

GC content: 30.73

Gene sequence:

>921_bases
GTGTATAAAGCTGGAAGTTTATCAATTAATGATATTTTAGAAAAAGAACCAGCTTTTGGTAAATTGGCGAATATAAAATC
TGAACAATTTTATAATATAGATAGTGTAGATATGACAATGGCAATGCGAATTAAGCTTGCTGAGTATGTACAAAAGCTAA
TAGATGATCCAAATGTTAATGCTGTGATTATTACTCATGGTACTGATAGTATGGTACAAACAGCATTTTTCCTTAATCAA
GTTTTAGATGTAAAAAACCCTGTCATTTTAGTAGGCGCTATGAGAGCTTTTACATTATTAAGTAGTGATGCGCTTATGAA
TTTATATGATGCTTTAGTTACTGCTGTAAACAAACAGTCAATTGGCAAGGGCATCTTAGTTGTGATGAATGAAGAAATTT
TAAATGCAAATGATGTTGCTAAGACTAATACAACAAATGTTGATGCGTTTAAAGCTCCAAATTATGGTAAATTAGGTACA
GTAATAATTAATGATGTTGATTATTATCAAAGTCCTAGGGTTGTTGGTAATATATTGAGTGTTGATGATTTACAAAAAAT
AACTGCCTTACCTAAAGTTGAGGTAATTTATGAATCAGCTGATATATCACCAGAGTTTTTAGATAGTGTCTTGACGATCA
AAGGCTTAGAGGGTATAGTTCTTGCAGGTCTTGGTGATGGCAATATTCTATCTAATCAAGGAGATTTTCTTAAAAAAGCA
CGAGCTAAGGGTATTGTAGTAGTTCGCTCTAGTTATGTCGGTTCTGGTAAAGTTACTCATAACTATAATAATTTGGATGA
CAAATTTGATCTTGTATCATCAGCAACACTTTCACCAGAAAAAGCGAGAATATTTTTACAGTTATGTTTAATAAAAACTC
ATGATATTAAAAAAATTCAAAAACTATTTGATAGATTTTAG

Upstream 100 bases:

>100_bases
TCTATCATCATATTTAGTCGCTCTAGCAGCTTTACCAAATGTATACATTGTTGCAACTGGTGGAACTATTGCAGGGGTTG
CAAAATCATCAACAGCAGCT

Downstream 100 bases:

>100_bases
TGGCTGAATTGATACAGTTTATTAGGCATCAGATAATATATTTGTCATCTTAAACTAGTTTTAGGATTTCAATAAAATCA
ATACTTACAGTAAAATGCTG

Product: periplasmic L-asparaginase II precursor

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MYKAGSLSINDILEKEPAFGKLANIKSEQFYNIDSVDMTMAMRIKLAEYVQKLIDDPNVNAVIITHGTDSMVQTAFFLNQ
VLDVKNPVILVGAMRAFTLLSSDALMNLYDALVTAVNKQSIGKGILVVMNEEILNANDVAKTNTTNVDAFKAPNYGKLGT
VIINDVDYYQSPRVVGNILSVDDLQKITALPKVEVIYESADISPEFLDSVLTIKGLEGIVLAGLGDGNILSNQGDFLKKA
RAKGIVVVRSSYVGSGKVTHNYNNLDDKFDLVSSATLSPEKARIFLQLCLIKTHDIKKIQKLFDRF

Sequences:

>Translated_306_residues
MYKAGSLSINDILEKEPAFGKLANIKSEQFYNIDSVDMTMAMRIKLAEYVQKLIDDPNVNAVIITHGTDSMVQTAFFLNQ
VLDVKNPVILVGAMRAFTLLSSDALMNLYDALVTAVNKQSIGKGILVVMNEEILNANDVAKTNTTNVDAFKAPNYGKLGT
VIINDVDYYQSPRVVGNILSVDDLQKITALPKVEVIYESADISPEFLDSVLTIKGLEGIVLAGLGDGNILSNQGDFLKKA
RAKGIVVVRSSYVGSGKVTHNYNNLDDKFDLVSSATLSPEKARIFLQLCLIKTHDIKKIQKLFDRF
>Mature_306_residues
MYKAGSLSINDILEKEPAFGKLANIKSEQFYNIDSVDMTMAMRIKLAEYVQKLIDDPNVNAVIITHGTDSMVQTAFFLNQ
VLDVKNPVILVGAMRAFTLLSSDALMNLYDALVTAVNKQSIGKGILVVMNEEILNANDVAKTNTTNVDAFKAPNYGKLGT
VIINDVDYYQSPRVVGNILSVDDLQKITALPKVEVIYESADISPEFLDSVLTIKGLEGIVLAGLGDGNILSNQGDFLKKA
RAKGIVVVRSSYVGSGKVTHNYNNLDDKFDLVSSATLSPEKARIFLQLCLIKTHDIKKIQKLFDRF

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=305, Percent_Identity=39.672131147541, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI1788065, Length=234, Percent_Identity=30.7692307692308, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17558278, Length=241, Percent_Identity=26.9709543568465, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17558280, Length=241, Percent_Identity=26.9709543568465, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI193207374, Length=241, Percent_Identity=26.9709543568465, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193207372, Length=276, Percent_Identity=25.7246376811594, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323189, Length=307, Percent_Identity=31.2703583061889, Blast_Score=156, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6323187, Length=307, Percent_Identity=31.2703583061889, Blast_Score=156, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6323186, Length=307, Percent_Identity=31.2703583061889, Blast_Score=156, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6323184, Length=307, Percent_Identity=31.2703583061889, Blast_Score=156, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6320527, Length=302, Percent_Identity=32.4503311258278, Blast_Score=150, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 33599; Mature: 33599

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKAGSLSINDILEKEPAFGKLANIKSEQFYNIDSVDMTMAMRIKLAEYVQKLIDDPNVN
CCCCCCCCHHHHHHCCCCCCHHHCCCCHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCC
AVIITHGTDSMVQTAFFLNQVLDVKNPVILVGAMRAFTLLSSDALMNLYDALVTAVNKQS
EEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGKGILVVMNEEILNANDVAKTNTTNVDAFKAPNYGKLGTVIINDVDYYQSPRVVGNILS
CCCCEEEEEECCCCCCCCCCCCCCCCCCEECCCCCCCCEEEEEECCHHHCCCHHHHHHCC
VDDLQKITALPKVEVIYESADISPEFLDSVLTIKGLEGIVLAGLGDGNILSNQGDFLKKA
HHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCEECCCCHHHHHH
RAKGIVVVRSSYVGSGKVTHNYNNLDDKFDLVSSATLSPEKARIFLQLCLIKTHDIKKIQ
HCCCEEEEEECCCCCCCEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHH
KLFDRF
HHHHCC
>Mature Secondary Structure
MYKAGSLSINDILEKEPAFGKLANIKSEQFYNIDSVDMTMAMRIKLAEYVQKLIDDPNVN
CCCCCCCCHHHHHHCCCCCCHHHCCCCHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCC
AVIITHGTDSMVQTAFFLNQVLDVKNPVILVGAMRAFTLLSSDALMNLYDALVTAVNKQS
EEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGKGILVVMNEEILNANDVAKTNTTNVDAFKAPNYGKLGTVIINDVDYYQSPRVVGNILS
CCCCEEEEEECCCCCCCCCCCCCCCCCCEECCCCCCCCEEEEEECCHHHCCCHHHHHHCC
VDDLQKITALPKVEVIYESADISPEFLDSVLTIKGLEGIVLAGLGDGNILSNQGDFLKKA
HHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCEECCCCHHHHHH
RAKGIVVVRSSYVGSGKVTHNYNNLDDKFDLVSSATLSPEKARIFLQLCLIKTHDIKKIQ
HCCCEEEEEECCCCCCCEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHH
KLFDRF
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8898907; 14500908 [H]