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The map label for this gene is def2 [H]

Identifier: 89255883

GI number: 89255883

Start: 449483

End: 450118

Strand: Direct

Name: def2 [H]

Synonym: FTL_0473

Alternate gene names: 89255883

Gene position: 449483-450118 (Clockwise)

Preceding gene: 89255882

Following gene: 89255884

Centisome position: 23.71

GC content: 31.6

Gene sequence:

>636_bases
ATGATGGTTGTAAATATCAAAATGCAACAAATGAAATCCCAATTTATACAGTATAACGACTCAAACAATAAGGTCTTGTA
CCAAAAATGTAAACCTGTAGCAGATATTCAAAATGCTGAAATACAAAATATTATTACAGAAATGCATGAAAAAATGCAAG
GTAATGGTATAGGATTGGCTGCTAATCAAATTGGTTATCCTTACCAAATATTTATGATAGAGTTTGATAGCTCAAATGCA
AGATATCCATTTAGCTTTGATAGTGTACCATATCAGGTGTTTATAAATCCTAAAATTACAAAAGCCTCAAAACAAAGAGT
AAGTTTTTGGCATGGATGTTTGAGTGCCTTAGGTGAGAAAAGAGGCAAGCTAGCTACTTATAAAGAGATTGAATATGAGG
CATATAACCAACACGGTGAGAAAATTACAGGTAAACTTGACTCTATAGCAGCTGTAATATTTCAGCATGAATTTAATCAT
CTACTTGGCTCTGTCTATGTTGATTTTGATACTGAGTATATCGATAACGAAGAACTACAAGCTAAATTTGCTAGTGGTGA
ACTTAAACCTTACCAAGAATGTGGTGAAGAAGTTCCACTATTATTAGAAAAATACCAAATAGGTAAAAATATATAA

Upstream 100 bases:

>100_bases
CACAGTTAGTTTGAGTGGGGTTTAATAATTTTGAATACTACTGCTGGAGTCGCAGTATTGATCTAGTAAAACTTTGCTAT
AATCTTTAACATACAAGATT

Downstream 100 bases:

>100_bases
GTTTTTTTAGTAATATCAAAACTAATAGTTGACTTATGAATTAAGAAGAGTATTATAGCTTGCATTGACGATGATAGCAG
TCTGGAACCACCTGATCCCA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MMVVNIKMQQMKSQFIQYNDSNNKVLYQKCKPVADIQNAEIQNIITEMHEKMQGNGIGLAANQIGYPYQIFMIEFDSSNA
RYPFSFDSVPYQVFINPKITKASKQRVSFWHGCLSALGEKRGKLATYKEIEYEAYNQHGEKITGKLDSIAAVIFQHEFNH
LLGSVYVDFDTEYIDNEELQAKFASGELKPYQECGEEVPLLLEKYQIGKNI

Sequences:

>Translated_211_residues
MMVVNIKMQQMKSQFIQYNDSNNKVLYQKCKPVADIQNAEIQNIITEMHEKMQGNGIGLAANQIGYPYQIFMIEFDSSNA
RYPFSFDSVPYQVFINPKITKASKQRVSFWHGCLSALGEKRGKLATYKEIEYEAYNQHGEKITGKLDSIAAVIFQHEFNH
LLGSVYVDFDTEYIDNEELQAKFASGELKPYQECGEEVPLLLEKYQIGKNI
>Mature_211_residues
MMVVNIKMQQMKSQFIQYNDSNNKVLYQKCKPVADIQNAEIQNIITEMHEKMQGNGIGLAANQIGYPYQIFMIEFDSSNA
RYPFSFDSVPYQVFINPKITKASKQRVSFWHGCLSALGEKRGKLATYKEIEYEAYNQHGEKITGKLDSIAAVIFQHEFNH
LLGSVYVDFDTEYIDNEELQAKFASGELKPYQECGEEVPLLLEKYQIGKNI

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=146, Percent_Identity=30.8219178082192, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 24262; Mature: 24262

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMVVNIKMQQMKSQFIQYNDSNNKVLYQKCKPVADIQNAEIQNIITEMHEKMQGNGIGLA
CEEEEEHHHHHHHHHHEECCCCCEEEHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEE
ANQIGYPYQIFMIEFDSSNARYPFSFDSVPYQVFINPKITKASKQRVSFWHGCLSALGEK
HHCCCCCEEEEEEEECCCCCCCCEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
RGKLATYKEIEYEAYNQHGEKITGKLDSIAAVIFQHEFNHLLGSVYVDFDTEYIDNEELQ
CCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHH
AKFASGELKPYQECGEEVPLLLEKYQIGKNI
HHHCCCCCCHHHHCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMVVNIKMQQMKSQFIQYNDSNNKVLYQKCKPVADIQNAEIQNIITEMHEKMQGNGIGLA
CEEEEEHHHHHHHHHHEECCCCCEEEHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEE
ANQIGYPYQIFMIEFDSSNARYPFSFDSVPYQVFINPKITKASKQRVSFWHGCLSALGEK
HHCCCCCEEEEEEEECCCCCCCCEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
RGKLATYKEIEYEAYNQHGEKITGKLDSIAAVIFQHEFNHLLGSVYVDFDTEYIDNEELQ
CCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHH
AKFASGELKPYQECGEEVPLLLEKYQIGKNI
HHHCCCCCCHHHHCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]