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The map label for this gene is folD [H]

Identifier: 89255805

GI number: 89255805

Start: 363556

End: 364404

Strand: Direct

Name: folD [H]

Synonym: FTL_0394

Alternate gene names: 89255805

Gene position: 363556-364404 (Clockwise)

Preceding gene: 89255804

Following gene: 89255806

Centisome position: 19.17

GC content: 37.46

Gene sequence:

>849_bases
ATGATTTTAATCGATGGTAAGTCTCTCTCAAAAGACCTTAAAGAGAGATTAGCAACTCAAGTTCAAGAATATAAGCATCA
TACAGCAATCACCCCAAAACTTGTCGCAATAATTGTTGGTAATGATCCGGCAAGTAAGACATATGTTGCCTCAAAAGAAA
AAGCCTGTGCCCAAGTTGGTATAGATTCTCAAGTGATTACACTACCAGAGCATACTACAGAATCAGAACTTTTGGAGCTG
ATCGATCAGCTAAATAATGACTCTAGTGTTCATGCGATTTTAGTGCAGCTACCATTACCAGCACATATCAACAAAAACAA
TGTGATATATAGTATAAAACCAGAAAAAGATGTCGATGGTTTTCATCCTACAAATGTTGGTAGACTACAACTTAGAGATA
AAAAATGCTTAGAATCTTGTACACCTAAAGGTATCATGACTATGCTAAGAGAGTATGGTATAAAAACAGAGGGGGCCTAT
GTAGTGGTTGTCGGCGCAAGTAATGTTGTTGGTAAACCAGTATCACAATTGCTGCTAAATGCTAAAGCTACAGTGACGAC
TTGTCATAGATTCACAACTGATCTTAAATCACATACTACAAAAGCAGATATCCTAATTGTTGCTGTTGGTAAGCCTAACT
TTATCACAGCTGATATGGTCAAAGAGGGTGCTGTAGTTATTGATGTTGGTATTAATCATGTTGATGGTAAGATAGTTGGT
GATGTTGATTTTGCTGCTGTAAAAGATAAGGTCGCAGCAATTACCCCAGTACCTGGAGGGGTTGGACCAATGACAATTAC
TGAACTGTTATATAATACTTTTCAGTGTGCTCAGGAGTTGAATAGGTAG

Upstream 100 bases:

>100_bases
AAACTTTATAAAAATACCCTCAAAAAAAATCCCAAAGATAGTCTAAAATTTGTTATCATATTAACTATATTTTTCAGTTA
TACAGATAAGCTATCAAATA

Downstream 100 bases:

>100_bases
TTTATAGATTTGATTTTATCATGGGTAGTCATACCCGCGTAGGCGAATGGGTTAAATCGAAAGGATTCCCGCCTGTGCGG
GAATGACAAGTGTTTTTAGA

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MILIDGKSLSKDLKERLATQVQEYKHHTAITPKLVAIIVGNDPASKTYVASKEKACAQVGIDSQVITLPEHTTESELLEL
IDQLNNDSSVHAILVQLPLPAHINKNNVIYSIKPEKDVDGFHPTNVGRLQLRDKKCLESCTPKGIMTMLREYGIKTEGAY
VVVVGASNVVGKPVSQLLLNAKATVTTCHRFTTDLKSHTTKADILIVAVGKPNFITADMVKEGAVVIDVGINHVDGKIVG
DVDFAAVKDKVAAITPVPGGVGPMTITELLYNTFQCAQELNR

Sequences:

>Translated_282_residues
MILIDGKSLSKDLKERLATQVQEYKHHTAITPKLVAIIVGNDPASKTYVASKEKACAQVGIDSQVITLPEHTTESELLEL
IDQLNNDSSVHAILVQLPLPAHINKNNVIYSIKPEKDVDGFHPTNVGRLQLRDKKCLESCTPKGIMTMLREYGIKTEGAY
VVVVGASNVVGKPVSQLLLNAKATVTTCHRFTTDLKSHTTKADILIVAVGKPNFITADMVKEGAVVIDVGINHVDGKIVG
DVDFAAVKDKVAAITPVPGGVGPMTITELLYNTFQCAQELNR
>Mature_282_residues
MILIDGKSLSKDLKERLATQVQEYKHHTAITPKLVAIIVGNDPASKTYVASKEKACAQVGIDSQVITLPEHTTESELLEL
IDQLNNDSSVHAILVQLPLPAHINKNNVIYSIKPEKDVDGFHPTNVGRLQLRDKKCLESCTPKGIMTMLREYGIKTEGAY
VVVVGASNVVGKPVSQLLLNAKATVTTCHRFTTDLKSHTTKADILIVAVGKPNFITADMVKEGAVVIDVGINHVDGKIVG
DVDFAAVKDKVAAITPVPGGVGPMTITELLYNTFQCAQELNR

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222418558, Length=295, Percent_Identity=46.7796610169492, Blast_Score=241, Evalue=7e-64,
Organism=Homo sapiens, GI94721354, Length=297, Percent_Identity=46.4646464646465, Blast_Score=234, Evalue=6e-62,
Organism=Homo sapiens, GI222136639, Length=289, Percent_Identity=43.598615916955, Blast_Score=232, Evalue=2e-61,
Organism=Homo sapiens, GI36796743, Length=212, Percent_Identity=27.8301886792453, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1786741, Length=278, Percent_Identity=53.2374100719424, Blast_Score=290, Evalue=6e-80,
Organism=Caenorhabditis elegans, GI17568735, Length=288, Percent_Identity=38.8888888888889, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6319558, Length=286, Percent_Identity=43.006993006993, Blast_Score=231, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6321643, Length=294, Percent_Identity=41.156462585034, Blast_Score=222, Evalue=7e-59,
Organism=Saccharomyces cerevisiae, GI6322933, Length=306, Percent_Identity=26.797385620915, Blast_Score=76, Evalue=5e-15,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=45.9183673469388, Blast_Score=238, Evalue=3e-63,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=45.9183673469388, Blast_Score=238, Evalue=4e-63,
Organism=Drosophila melanogaster, GI62472483, Length=288, Percent_Identity=40.2777777777778, Blast_Score=210, Evalue=1e-54,
Organism=Drosophila melanogaster, GI45551871, Length=288, Percent_Identity=40.2777777777778, Blast_Score=210, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24645718, Length=288, Percent_Identity=40.2777777777778, Blast_Score=209, Evalue=1e-54,
Organism=Drosophila melanogaster, GI17137370, Length=288, Percent_Identity=40.2777777777778, Blast_Score=209, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30483; Mature: 30483

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILIDGKSLSKDLKERLATQVQEYKHHTAITPKLVAIIVGNDPASKTYVASKEKACAQVG
CEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEECCHHHHHHHCC
IDSQVITLPEHTTESELLELIDQLNNDSSVHAILVQLPLPAHINKNNVIYSIKPEKDVDG
CCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCEECCCCEEEEECCCCCCCC
FHPTNVGRLQLRDKKCLESCTPKGIMTMLREYGIKTEGAYVVVVGASNVVGKPVSQLLLN
CCCCCCCEEEECHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHC
AKATVTTCHRFTTDLKSHTTKADILIVAVGKPNFITADMVKEGAVVIDVGINHVDGKIVG
CCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEHHHHHCCCEEEEECCCCCCCEEEE
DVDFAAVKDKVAAITPVPGGVGPMTITELLYNTFQCAQELNR
CCHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MILIDGKSLSKDLKERLATQVQEYKHHTAITPKLVAIIVGNDPASKTYVASKEKACAQVG
CEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEECCHHHHHHHCC
IDSQVITLPEHTTESELLELIDQLNNDSSVHAILVQLPLPAHINKNNVIYSIKPEKDVDG
CCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCEECCCCEEEEECCCCCCCC
FHPTNVGRLQLRDKKCLESCTPKGIMTMLREYGIKTEGAYVVVVGASNVVGKPVSQLLLN
CCCCCCCEEEECHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHC
AKATVTTCHRFTTDLKSHTTKADILIVAVGKPNFITADMVKEGAVVIDVGINHVDGKIVG
CCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEHHHHHCCCEEEEECCCCCCCEEEE
DVDFAAVKDKVAAITPVPGGVGPMTITELLYNTFQCAQELNR
CCHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA