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The map label for this gene is sacA [H]

Identifier: 89255500

GI number: 89255500

Start: 53270

End: 54202

Strand: Reverse

Name: sacA [H]

Synonym: FTL_0053

Alternate gene names: 89255500

Gene position: 54202-53270 (Counterclockwise)

Preceding gene: 89255502

Following gene: 89255499

Centisome position: 2.86

GC content: 33.01

Gene sequence:

>933_bases
TTGAATATTCAAAATGGTTGGATATATGATGCTAACTTACTTGAGACAGGTGGTATGTGCTTTAGCTTACGGGCGGATAG
CTTTAAATCTTACCATTCTCATGATAATAGTGATATACACAAAACTAAACGCTTGCAGTACCATTTTACTGCTAAAAAGG
CTTGGATGAATGATCCAAACGGTTTAGTTTACTTTAAAGGTAAATATCATATGTTTTACCAGCACTACCCTTATGGTAAA
CATACAGCAATGCTACACTGGGGACATGCTGTATCTACAGACCTTGTCAACTGGCAACATTTACCTATTGCAATTTATCC
ACAAAATGATCTTAATGATAGATATATCGGTGGCGCTTTTTCTGGTAGTGCTATGGTTGTCGATGATGAGCTTTTTCTTA
TTTATACCGAACATTTTGAAGATTTAGACAATACTCCAAATATCTTTATAGAAAAACAAAATCTAATAAAAAGTAAAGAT
GGTATTCACTTCTCAAAACCGAAAATAGTAATAAATCATAAGCCAGATTTTTGTTCTTATGACTTTAGAGATCCTAAAGT
ATGGTTTGATAAGATTTTTAACTGTTATTACATGGTGATTGGCACTTATGCAAATAACTATCCAAGTGTTGCACTGTATA
GATCTGATAATGCTGAGAGCTGGAATTATCATAGTATATTATTTCAAGAAAAAACTATCTCTGGAAGAACTCTAGAATGC
CCAGACCTGTTTTATTTAGATGGAAAATATGTATTGGTTTTATCAATTTTTGAGACAAAAAATAAGAAGGACTTTAATTA
TCAGTCATATTATTATATTGGAAACTTCGATGGTAAGCATTTCTCTGCAGAAACACCTCCTACTCTGCAAAAGAGTTTTA
TGCACCACAGACTTTTGAAGCTAATGGTAATAGATGCGCTATCGGTTGGATGA

Upstream 100 bases:

>100_bases
GATAATACCGACAAACCTAAAATCAAAATATTACCATGTTGAATGGTCTTTAGTAGCTGATCAAGAATATATTTTCGAAA
TATTTGGAGATATACAAAAC

Downstream 100 bases:

>100_bases
ACTGTTGGGAAGACTATAAAAACCGAGCTAAAGACGATTCGGCTGGGGCAATGAGTTTAATCAGACAGCTTAAAGTAAAG
AAAGATAAGCTTTTATCTTA

Product: Beta-fructofuranosidase

Products: NA

Alternate protein names: Sucrase; Invertase [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MNIQNGWIYDANLLETGGMCFSLRADSFKSYHSHDNSDIHKTKRLQYHFTAKKAWMNDPNGLVYFKGKYHMFYQHYPYGK
HTAMLHWGHAVSTDLVNWQHLPIAIYPQNDLNDRYIGGAFSGSAMVVDDELFLIYTEHFEDLDNTPNIFIEKQNLIKSKD
GIHFSKPKIVINHKPDFCSYDFRDPKVWFDKIFNCYYMVIGTYANNYPSVALYRSDNAESWNYHSILFQEKTISGRTLEC
PDLFYLDGKYVLVLSIFETKNKKDFNYQSYYYIGNFDGKHFSAETPPTLQKSFMHHRLLKLMVIDALSVG

Sequences:

>Translated_310_residues
MNIQNGWIYDANLLETGGMCFSLRADSFKSYHSHDNSDIHKTKRLQYHFTAKKAWMNDPNGLVYFKGKYHMFYQHYPYGK
HTAMLHWGHAVSTDLVNWQHLPIAIYPQNDLNDRYIGGAFSGSAMVVDDELFLIYTEHFEDLDNTPNIFIEKQNLIKSKD
GIHFSKPKIVINHKPDFCSYDFRDPKVWFDKIFNCYYMVIGTYANNYPSVALYRSDNAESWNYHSILFQEKTISGRTLEC
PDLFYLDGKYVLVLSIFETKNKKDFNYQSYYYIGNFDGKHFSAETPPTLQKSFMHHRLLKLMVIDALSVG
>Mature_310_residues
MNIQNGWIYDANLLETGGMCFSLRADSFKSYHSHDNSDIHKTKRLQYHFTAKKAWMNDPNGLVYFKGKYHMFYQHYPYGK
HTAMLHWGHAVSTDLVNWQHLPIAIYPQNDLNDRYIGGAFSGSAMVVDDELFLIYTEHFEDLDNTPNIFIEKQNLIKSKD
GIHFSKPKIVINHKPDFCSYDFRDPKVWFDKIFNCYYMVIGTYANNYPSVALYRSDNAESWNYHSILFQEKTISGRTLEC
PDLFYLDGKYVLVLSIFETKNKKDFNYQSYYYIGNFDGKHFSAETPPTLQKSFMHHRLLKLMVIDALSVG

Specific function: Unknown

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=268, Percent_Identity=30.9701492537313, Blast_Score=96, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR006232 [H]

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.26 [H]

Molecular weight: Translated: 36379; Mature: 36379

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIQNGWIYDANLLETGGMCFSLRADSFKSYHSHDNSDIHKTKRLQYHFTAKKAWMNDPN
CCCCCCEEEECCEEECCCEEEEEECCCHHHHHCCCCCCHHHHEEEEEEEEEHHHHCCCCC
GLVYFKGKYHMFYQHYPYGKHTAMLHWGHAVSTDLVNWQHLPIAIYPQNDLNDRYIGGAF
CEEEECCEEEEEEEECCCCCEEEEEEECCCHHHCCCCEEECCEEEECCCCCCCCEECCEE
SGSAMVVDDELFLIYTEHFEDLDNTPNIFIEKQNLIKSKDGIHFSKPKIVINHKPDFCSY
CCCEEEEECCEEEEEECHHHCCCCCCCEEEECCHHHCCCCCCEECCCEEEEECCCCCCCC
DFRDPKVWFDKIFNCYYMVIGTYANNYPSVALYRSDNAESWNYHSILFQEKTISGRTLEC
CCCCCHHHHHHHHHEEEEEEECCCCCCCCEEEEECCCCCCCEEEEEEEEECCCCCCEECC
PDLFYLDGKYVLVLSIFETKNKKDFNYQSYYYIGNFDGKHFSAETPPTLQKSFMHHRLLK
CCEEEECCCEEEEEEEEECCCCCCCCEEEEEEEECCCCCEECCCCCHHHHHHHHHHHHHH
LMVIDALSVG
HHHHHHHCCC
>Mature Secondary Structure
MNIQNGWIYDANLLETGGMCFSLRADSFKSYHSHDNSDIHKTKRLQYHFTAKKAWMNDPN
CCCCCCEEEECCEEECCCEEEEEECCCHHHHHCCCCCCHHHHEEEEEEEEEHHHHCCCCC
GLVYFKGKYHMFYQHYPYGKHTAMLHWGHAVSTDLVNWQHLPIAIYPQNDLNDRYIGGAF
CEEEECCEEEEEEEECCCCCEEEEEEECCCHHHCCCCEEECCEEEECCCCCCCCEECCEE
SGSAMVVDDELFLIYTEHFEDLDNTPNIFIEKQNLIKSKDGIHFSKPKIVINHKPDFCSY
CCCEEEEECCEEEEEECHHHCCCCCCCEEEECCHHHCCCCCCEECCCEEEEECCCCCCCC
DFRDPKVWFDKIFNCYYMVIGTYANNYPSVALYRSDNAESWNYHSILFQEKTISGRTLEC
CCCCCHHHHHHHHHEEEEEEECCCCCCCCEEEEECCCCCCCEEEEEEEEECCCCCCEECC
PDLFYLDGKYVLVLSIFETKNKKDFNYQSYYYIGNFDGKHFSAETPPTLQKSFMHHRLLK
CCEEEECCCEEEEEEEEECCCCCCCCEEEEEEEECCCCCEECCCCCHHHHHHHHHHHHHH
LMVIDALSVG
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2254250; 1368686 [H]