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The map label for this gene is TA-iaaH [H]

Identifier: 89056468

GI number: 89056468

Start: 4076868

End: 4078145

Strand: Direct

Name: TA-iaaH [H]

Synonym: Jann_3977

Alternate gene names: 89056468

Gene position: 4076868-4078145 (Clockwise)

Preceding gene: 89056467

Following gene: 89056469

Centisome position: 94.42

GC content: 62.99

Gene sequence:

>1278_bases
GTGAACGACAAGGTGACGGCGGCCAAAACTGCTTTGCGCGCCATGGCCGGCCTGAACCCGCTGGTGCGCGATCCGTTCGA
CCTGTCGACCGGAGGCGATGGCCCGTTGTCAGGTATGCCAATCGTGGTCAAAGCCAATATCGCCATAGACGAATTGCCCC
AGTGTGGCGCGTCCCCGGCCCTGGAGGACAACATCGCGCAGAACTCCGCGCCCGTTGTTCAAAGGCTGCTTGACGCCGGG
GCGGTCATTGTCGGGCAGGCAAACATGCACGAATTGGCGTTCGGCATCACCAGTTACAACGCGTATCACGGCGCGGTCGG
CAATCCCGCCAATCCCGCGCATATGGCCGGTGGCTCCTCTGGCGGGACGGCCGCTGCGGTTGCGTCCGGGGCGGTTTCCT
TGGGCCTGGCCTCGGACACCGGTGGATCCGGCAGATTGCCTGCCGCCATGTGTGGCTGTGTCGGGTTCCGGCCCACGCAG
GGGCGCTATCCATCGGACGGCATTCTCACGTTGTCGTCCAGCTTTGACACCGTCACGCCTATGGCACGTGATGTGGCGGG
ACTTACCCTGATCGACGCGGTGATGGCAGGCGTGGCACCCCGAAAGCGCGATCCGCACAAACCGCTGCGCCTCGGTGTGG
TGGAAGATGCCTTCTGGCAGGGCGTCGATGCGGCGATGGCGCAGGCATGTCGTGACAAGCTCAACACCCTTGAGGACGCG
GGCGTGCATCTCGTGCCGCTCAGCGCTCCGGATCTGATGGCCCGCTGCGCGGACATCTCGATGGGCATCGTTCTTTATGA
AACGCAGATCTACTGGGACGCTTTTCTTGCATCGCGGGACACCACAATGATCGCATTCGCAGATCAGATCGCCAGTCCGG
ATGTCGCCAGCATTTTTGGTCACGTCGCCAGTGGTGCGGCCCCGTCCGATGCCGACTACGCGCAAATGAGCGGCCCCAAG
CGCCGAGCAATTTGCAAGATCGTGACTGATCTGCTGGCTGACGTCGACGGCCTGATCATGCCGACACTGGCCACCACCGC
CCCGCTGATCGACCAGACCGAGACCTGCCGGATCAATGGCGAAGAGGTTGACCTATTCAACGCTCTGACGGCGCGGGCCC
TTGTTGCGTCCATCACTGGCCATCCCGAGATCTCCCTGCCTGCGGGGCAGCTGGGCGGGTTGCCCATCGGGTTGGGCATA
TTAGGGACGCATGGCCAGGATGATGAATTGCTGGCGGTTGCGGCACAGATCGAACCCCTGTTGGCAGCAACGAATTGA

Upstream 100 bases:

>100_bases
TCAAGCCCGCCGCACAGATCGTGGAGGAGATGATGGACGGGGCAAACGACCTTCTTAATCGCATGGGCGCGTCGATCCCG
ACGGCAGCGGAGTGACCGCC

Downstream 100 bases:

>100_bases
TCATGAGGGGGGCTAGGCAAGATCGGCGGCGGCTCAACTAGGGCCTGTCATCGCCGGTCTGCGCGTCGGGCCGGAGATGA
TCGGGATCCTGTTACGGCTC

Product: amidase

Products: NA

Alternate protein names: IAH; Indole-3-acetamide hydrolase [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MNDKVTAAKTALRAMAGLNPLVRDPFDLSTGGDGPLSGMPIVVKANIAIDELPQCGASPALEDNIAQNSAPVVQRLLDAG
AVIVGQANMHELAFGITSYNAYHGAVGNPANPAHMAGGSSGGTAAAVASGAVSLGLASDTGGSGRLPAAMCGCVGFRPTQ
GRYPSDGILTLSSSFDTVTPMARDVAGLTLIDAVMAGVAPRKRDPHKPLRLGVVEDAFWQGVDAAMAQACRDKLNTLEDA
GVHLVPLSAPDLMARCADISMGIVLYETQIYWDAFLASRDTTMIAFADQIASPDVASIFGHVASGAAPSDADYAQMSGPK
RRAICKIVTDLLADVDGLIMPTLATTAPLIDQTETCRINGEEVDLFNALTARALVASITGHPEISLPAGQLGGLPIGLGI
LGTHGQDDELLAVAAQIEPLLAATN

Sequences:

>Translated_425_residues
MNDKVTAAKTALRAMAGLNPLVRDPFDLSTGGDGPLSGMPIVVKANIAIDELPQCGASPALEDNIAQNSAPVVQRLLDAG
AVIVGQANMHELAFGITSYNAYHGAVGNPANPAHMAGGSSGGTAAAVASGAVSLGLASDTGGSGRLPAAMCGCVGFRPTQ
GRYPSDGILTLSSSFDTVTPMARDVAGLTLIDAVMAGVAPRKRDPHKPLRLGVVEDAFWQGVDAAMAQACRDKLNTLEDA
GVHLVPLSAPDLMARCADISMGIVLYETQIYWDAFLASRDTTMIAFADQIASPDVASIFGHVASGAAPSDADYAQMSGPK
RRAICKIVTDLLADVDGLIMPTLATTAPLIDQTETCRINGEEVDLFNALTARALVASITGHPEISLPAGQLGGLPIGLGI
LGTHGQDDELLAVAAQIEPLLAATN
>Mature_425_residues
MNDKVTAAKTALRAMAGLNPLVRDPFDLSTGGDGPLSGMPIVVKANIAIDELPQCGASPALEDNIAQNSAPVVQRLLDAG
AVIVGQANMHELAFGITSYNAYHGAVGNPANPAHMAGGSSGGTAAAVASGAVSLGLASDTGGSGRLPAAMCGCVGFRPTQ
GRYPSDGILTLSSSFDTVTPMARDVAGLTLIDAVMAGVAPRKRDPHKPLRLGVVEDAFWQGVDAAMAQACRDKLNTLEDA
GVHLVPLSAPDLMARCADISMGIVLYETQIYWDAFLASRDTTMIAFADQIASPDVASIFGHVASGAAPSDADYAQMSGPK
RRAICKIVTDLLADVDGLIMPTLATTAPLIDQTETCRINGEEVDLFNALTARALVASITGHPEISLPAGQLGGLPIGLGI
LGTHGQDDELLAVAAQIEPLLAATN

Specific function: Hydrolyzes indole-3-acetamide (IAM) into indole-3-acetic acid (IAA) [H]

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=415, Percent_Identity=26.5060240963855, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI222831590, Length=446, Percent_Identity=26.6816143497758, Blast_Score=93, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17543272, Length=428, Percent_Identity=25.7009345794392, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17537465, Length=420, Percent_Identity=24.7619047619048, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71990152, Length=264, Percent_Identity=27.6515151515151, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17556264, Length=128, Percent_Identity=32.8125, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17538252, Length=249, Percent_Identity=25.3012048192771, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17556276, Length=134, Percent_Identity=37.3134328358209, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=134, Percent_Identity=37.3134328358209, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=402, Percent_Identity=27.363184079602, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6323950, Length=470, Percent_Identity=23.8297872340426, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648441, Length=408, Percent_Identity=27.4509803921569, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24648439, Length=408, Percent_Identity=27.4509803921569, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24648435, Length=408, Percent_Identity=27.4509803921569, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648437, Length=408, Percent_Identity=27.4509803921569, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI45550774, Length=408, Percent_Identity=27.4509803921569, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24652985, Length=430, Percent_Identity=26.046511627907, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19922090, Length=430, Percent_Identity=26.046511627907, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24652981, Length=430, Percent_Identity=26.046511627907, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24652983, Length=430, Percent_Identity=26.046511627907, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24644968, Length=424, Percent_Identity=26.1792452830189, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648113, Length=429, Percent_Identity=27.5058275058275, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356731, Length=407, Percent_Identity=25.3071253071253, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI161078093, Length=417, Percent_Identity=24.7002398081535, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 43539; Mature: 43539

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDKVTAAKTALRAMAGLNPLVRDPFDLSTGGDGPLSGMPIVVKANIAIDELPQCGASPA
CCCCHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCEEEEECCCHHHCCCCCCCCC
LEDNIAQNSAPVVQRLLDAGAVIVGQANMHELAFGITSYNAYHGAVGNPANPAHMAGGSS
CHHHHCCCCHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCC
GGTAAAVASGAVSLGLASDTGGSGRLPAAMCGCVGFRPTQGRYPSDGILTLSSSFDTVTP
CCCHHHHHHCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEECCCCHHHH
MARDVAGLTLIDAVMAGVAPRKRDPHKPLRLGVVEDAFWQGVDAAMAQACRDKLNTLEDA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHC
GVHLVPLSAPDLMARCADISMGIVLYETQIYWDAFLASRDTTMIAFADQIASPDVASIFG
CEEEEECCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCCCEEEEEHHHHCCCCHHHHHH
HVASGAAPSDADYAQMSGPKRRAICKIVTDLLADVDGLIMPTLATTAPLIDQTETCRING
HHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEECC
EEVDLFNALTARALVASITGHPEISLPAGQLGGLPIGLGILGTHGQDDELLAVAAQIEPL
CHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCEEEEEEEECCCCCCCHHEEHHHHHCCH
LAATN
HCCCC
>Mature Secondary Structure
MNDKVTAAKTALRAMAGLNPLVRDPFDLSTGGDGPLSGMPIVVKANIAIDELPQCGASPA
CCCCHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCEEEEECCCHHHCCCCCCCCC
LEDNIAQNSAPVVQRLLDAGAVIVGQANMHELAFGITSYNAYHGAVGNPANPAHMAGGSS
CHHHHCCCCHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCC
GGTAAAVASGAVSLGLASDTGGSGRLPAAMCGCVGFRPTQGRYPSDGILTLSSSFDTVTP
CCCHHHHHHCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEECCCCHHHH
MARDVAGLTLIDAVMAGVAPRKRDPHKPLRLGVVEDAFWQGVDAAMAQACRDKLNTLEDA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHC
GVHLVPLSAPDLMARCADISMGIVLYETQIYWDAFLASRDTTMIAFADQIASPDVASIFG
CEEEEECCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCCCEEEEEHHHHCCCCHHHHHH
HVASGAAPSDADYAQMSGPKRRAICKIVTDLLADVDGLIMPTLATTAPLIDQTETCRING
HHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEECC
EEVDLFNALTARALVASITGHPEISLPAGQLGGLPIGLGILGTHGQDDELLAVAAQIEPL
CHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCEEEEEEEECCCCCCCHHEEHHHHHCCH
LAATN
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1868204 [H]