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The map label for this gene is alkJ [H]

Identifier: 89056171

GI number: 89056171

Start: 3757453

End: 3759066

Strand: Direct

Name: alkJ [H]

Synonym: Jann_3680

Alternate gene names: 89056171

Gene position: 3757453-3759066 (Clockwise)

Preceding gene: 89056165

Following gene: 89056172

Centisome position: 87.02

GC content: 63.51

Gene sequence:

>1614_bases
ATGACCGAAGTATCAGCGGATTACGTGATTGTTGGCGCGGGCTCCGCGGGATGCGTGCTGGCCAACCGGCTATCGGCTGA
TTCGCGAAACTCTGTCGTTCTGCTGGAAGCGGGTGGTCGGGACTGGAACCCATGGATCCACATCCCCGTGGGCTATTTCA
AGACGATCCATAACCCGTCGGTGGACTGGTGCTACAAGACCGAGCCTGATCCCGGCCTGAATGGGCGGTCCATCGAATGG
CCAAGAGGCAAGGTCCTGGGGGGATCATCGTCGCTGAACGGCCTGCTCTATGTGCGTGGACAAGCCCAGGATTACGACCG
CTGGCGGCAGATGGGCAACGCGGGATGGGCGTGGGATGATGTGCTGCCGCTGTTCAAGCGGGCCGAGCACAATGAGCGGG
GCGCCGATGAATTCCACGGCGACGAAGGGCCGCTTTCGGTCTCCAACATGCGCATCCAGCGGCCCATCACCGACGCCTGG
GTCGCGGCAGCTCAGGCGGCTGGCTATCCGTTCAACCCCGACTACAACGGCAAAAGCCAGGAAGGTGTGGGGTATTTTCA
ACTCACCTCCCGCAACGGGCGGCGGTGCAGCTCTGCGGTGGCTTATCTCAACCCCGCCCGCTCCCGCGAGAACCTGCGGA
TCATCACCCACGCACAGGTCGACCGCGTTGTCCTGGACGGCAAACGCGCCACGGGCGTGGCCTACACGGACCGATCCGGC
ACCCTGGTGACGGTCAAAGCGGGCAAAGAGGTGATCCTGTGCGGCGGGGCCATCAACTCTCCCCAATTGTTGATGACATC
GGGCATTGGGGAGGCTGCGCACCTGGCCGAACACGGGATAGACGTGGTCCAGGACCTGCACGGCGTGGGCAAGAACATGC
AGGACCATTTGCAGGCCCGGCTGGTCTACAAATGCAACGAGCCGACCCTTAATGACGAGGTGTCCAGCCTTTATGGTCAG
GCGCGGATCGGGTTGAAATACCTCATGTTCCGCGCCGGGCCGATGACCATGGCCGCGAGCCTTGCGACCGGGTTCATGCG
TACCCGCGACGATCTGGAAACGCCGGACATACAATTCCATGTCCAGCCCCTTTCGGCCGAGAACCCCGGCAAGGGCGCGG
ACAAGTTCAGCGCCTTCACCATGTCCGTGTGCCAGCTGCGCCCCGAAAGCCGGGGAGAGATCCGCTTGGCCAGCGCCAAC
CCCCGCACCTACCCCCGGATCATCCCCAATTATCTGTCGACGGAGACCGATTGCCGGACCATCGTGGCCGGGGTCAACAT
CGCGCGGACCATCGCGCGCCATGCGCCCCTGACGTCCAAGATCAGCGAAGAATTCCGTCCCGACCCCAGCCTCGACATCA
ACGATTACGGCGCAACGCTGGATTGGGCCCGCAACAACACCGCCTCGATCTATCACCCCACCGGCACCTGCAAGATGGGC
CCGGGCCCGGACGCGGTGGTCGATGCCCGCCTGCGCGTCCACGGGATCAGCGGGCTGCGGGTTGCGGATTGCTCGATCAT
GCCGGAAATCGTGTCGGGCAACACCAACGCGCCCGCGATCATGATTGGCGAAAAGGCCTCGGACCTGATCCTGGAAGACG
CAGCGGTCGCATAG

Upstream 100 bases:

>100_bases
GCAAAATTGATACTTGTGGTCTCAATCTTGCTCAACTTATGGTCGCAAGAGCAAGGAAAAACTTGTTTATGCCGAAAATC
TAACCAAGGGGCGCCCGACA

Downstream 100 bases:

>100_bases
GCGTGCCCCCACCCCACAACTCATGAACAATACCTTTGTGTTGCCTCAATTGGCCTCAGTTTCGTCATGTCGCCATGACT
ACTTTTCGCAAGTTTGAGCA

Product: glucose-methanol-choline oxidoreductase

Products: betaine aldehyde; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MTEVSADYVIVGAGSAGCVLANRLSADSRNSVVLLEAGGRDWNPWIHIPVGYFKTIHNPSVDWCYKTEPDPGLNGRSIEW
PRGKVLGGSSSLNGLLYVRGQAQDYDRWRQMGNAGWAWDDVLPLFKRAEHNERGADEFHGDEGPLSVSNMRIQRPITDAW
VAAAQAAGYPFNPDYNGKSQEGVGYFQLTSRNGRRCSSAVAYLNPARSRENLRIITHAQVDRVVLDGKRATGVAYTDRSG
TLVTVKAGKEVILCGGAINSPQLLMTSGIGEAAHLAEHGIDVVQDLHGVGKNMQDHLQARLVYKCNEPTLNDEVSSLYGQ
ARIGLKYLMFRAGPMTMAASLATGFMRTRDDLETPDIQFHVQPLSAENPGKGADKFSAFTMSVCQLRPESRGEIRLASAN
PRTYPRIIPNYLSTETDCRTIVAGVNIARTIARHAPLTSKISEEFRPDPSLDINDYGATLDWARNNTASIYHPTGTCKMG
PGPDAVVDARLRVHGISGLRVADCSIMPEIVSGNTNAPAIMIGEKASDLILEDAAVA

Sequences:

>Translated_537_residues
MTEVSADYVIVGAGSAGCVLANRLSADSRNSVVLLEAGGRDWNPWIHIPVGYFKTIHNPSVDWCYKTEPDPGLNGRSIEW
PRGKVLGGSSSLNGLLYVRGQAQDYDRWRQMGNAGWAWDDVLPLFKRAEHNERGADEFHGDEGPLSVSNMRIQRPITDAW
VAAAQAAGYPFNPDYNGKSQEGVGYFQLTSRNGRRCSSAVAYLNPARSRENLRIITHAQVDRVVLDGKRATGVAYTDRSG
TLVTVKAGKEVILCGGAINSPQLLMTSGIGEAAHLAEHGIDVVQDLHGVGKNMQDHLQARLVYKCNEPTLNDEVSSLYGQ
ARIGLKYLMFRAGPMTMAASLATGFMRTRDDLETPDIQFHVQPLSAENPGKGADKFSAFTMSVCQLRPESRGEIRLASAN
PRTYPRIIPNYLSTETDCRTIVAGVNIARTIARHAPLTSKISEEFRPDPSLDINDYGATLDWARNNTASIYHPTGTCKMG
PGPDAVVDARLRVHGISGLRVADCSIMPEIVSGNTNAPAIMIGEKASDLILEDAAVA
>Mature_536_residues
TEVSADYVIVGAGSAGCVLANRLSADSRNSVVLLEAGGRDWNPWIHIPVGYFKTIHNPSVDWCYKTEPDPGLNGRSIEWP
RGKVLGGSSSLNGLLYVRGQAQDYDRWRQMGNAGWAWDDVLPLFKRAEHNERGADEFHGDEGPLSVSNMRIQRPITDAWV
AAAQAAGYPFNPDYNGKSQEGVGYFQLTSRNGRRCSSAVAYLNPARSRENLRIITHAQVDRVVLDGKRATGVAYTDRSGT
LVTVKAGKEVILCGGAINSPQLLMTSGIGEAAHLAEHGIDVVQDLHGVGKNMQDHLQARLVYKCNEPTLNDEVSSLYGQA
RIGLKYLMFRAGPMTMAASLATGFMRTRDDLETPDIQFHVQPLSAENPGKGADKFSAFTMSVCQLRPESRGEIRLASANP
RTYPRIIPNYLSTETDCRTIVAGVNIARTIARHAPLTSKISEEFRPDPSLDINDYGATLDWARNNTASIYHPTGTCKMGP
GPDAVVDARLRVHGISGLRVADCSIMPEIVSGNTNAPAIMIGEKASDLILEDAAVA

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=535, Percent_Identity=44.1121495327103, Blast_Score=419, Evalue=1e-117,
Organism=Escherichia coli, GI1786503, Length=531, Percent_Identity=42.1845574387947, Blast_Score=402, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17532301, Length=555, Percent_Identity=37.6576576576577, Blast_Score=357, Evalue=9e-99,
Organism=Drosophila melanogaster, GI24642059, Length=569, Percent_Identity=40.597539543058, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24642042, Length=576, Percent_Identity=40.1041666666667, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI45551458, Length=571, Percent_Identity=38.5288966725044, Blast_Score=346, Evalue=2e-95,
Organism=Drosophila melanogaster, GI45549471, Length=571, Percent_Identity=38.5288966725044, Blast_Score=346, Evalue=2e-95,
Organism=Drosophila melanogaster, GI24650267, Length=565, Percent_Identity=38.7610619469027, Blast_Score=335, Evalue=5e-92,
Organism=Drosophila melanogaster, GI24642048, Length=577, Percent_Identity=37.6083188908146, Blast_Score=332, Evalue=4e-91,
Organism=Drosophila melanogaster, GI17137792, Length=554, Percent_Identity=38.2671480144404, Blast_Score=330, Evalue=2e-90,
Organism=Drosophila melanogaster, GI18859995, Length=569, Percent_Identity=39.0158172231986, Blast_Score=325, Evalue=4e-89,
Organism=Drosophila melanogaster, GI24642039, Length=576, Percent_Identity=38.1944444444444, Blast_Score=323, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24642055, Length=585, Percent_Identity=37.0940170940171, Blast_Score=319, Evalue=3e-87,
Organism=Drosophila melanogaster, GI24642035, Length=580, Percent_Identity=36.551724137931, Blast_Score=297, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24642037, Length=573, Percent_Identity=36.3001745200698, Blast_Score=295, Evalue=4e-80,
Organism=Drosophila melanogaster, GI24642051, Length=571, Percent_Identity=34.8511383537653, Blast_Score=290, Evalue=1e-78,
Organism=Drosophila melanogaster, GI18859993, Length=573, Percent_Identity=35.6020942408377, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24642057, Length=579, Percent_Identity=29.706390328152, Blast_Score=178, Evalue=8e-45,
Organism=Drosophila melanogaster, GI24645930, Length=580, Percent_Identity=28.6206896551724, Blast_Score=158, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1

Molecular weight: Translated: 58348; Mature: 58217

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEVSADYVIVGAGSAGCVLANRLSADSRNSVVLLEAGGRDWNPWIHIPVGYFKTIHNPS
CCCCCCCEEEEECCCCCEEEEHHCCCCCCCCEEEEECCCCCCCCEEEEEHHHHHHCCCCC
VDWCYKTEPDPGLNGRSIEWPRGKVLGGSSSLNGLLYVRGQAQDYDRWRQMGNAGWAWDD
CCEEEECCCCCCCCCCEEECCCCEEECCCCCCCEEEEEECCCCCHHHHHHCCCCCCCHHH
VLPLFKRAEHNERGADEFHGDEGPLSVSNMRIQRPITDAWVAAAQAAGYPFNPDYNGKSQ
HHHHHHHHCCCCCCCHHHCCCCCCEEECCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCC
EGVGYFQLTSRNGRRCSSAVAYLNPARSRENLRIITHAQVDRVVLDGKRATGVAYTDRSG
CCCEEEEEECCCCCCHHHHHHHCCCCCCCCCEEEEEECCCCEEEECCCCCCCEEEECCCC
TLVTVKAGKEVILCGGAINSPQLLMTSGIGEAAHLAEHGIDVVQDLHGVGKNMQDHLQAR
CEEEEECCCEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHEE
LVYKCNEPTLNDEVSSLYGQARIGLKYLMFRAGPMTMAASLATGFMRTRDDLETPDIQFH
EEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEE
VQPLSAENPGKGADKFSAFTMSVCQLRPESRGEIRLASANPRTYPRIIPNYLSTETDCRT
EEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHCCHHHCCCCCHHH
IVAGVNIARTIARHAPLTSKISEEFRPDPSLDINDYGATLDWARNNTASIYHPTGTCKMG
HHHCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCEEEECCCCEECC
PGPDAVVDARLRVHGISGLRVADCSIMPEIVSGNTNAPAIMIGEKASDLILEDAAVA
CCCCCEEEEEEEEECCCCCEEECCCCCHHHHCCCCCCCEEEECCCCCCHHHHHCCCC
>Mature Secondary Structure 
TEVSADYVIVGAGSAGCVLANRLSADSRNSVVLLEAGGRDWNPWIHIPVGYFKTIHNPS
CCCCCCEEEEECCCCCEEEEHHCCCCCCCCEEEEECCCCCCCCEEEEEHHHHHHCCCCC
VDWCYKTEPDPGLNGRSIEWPRGKVLGGSSSLNGLLYVRGQAQDYDRWRQMGNAGWAWDD
CCEEEECCCCCCCCCCEEECCCCEEECCCCCCCEEEEEECCCCCHHHHHHCCCCCCCHHH
VLPLFKRAEHNERGADEFHGDEGPLSVSNMRIQRPITDAWVAAAQAAGYPFNPDYNGKSQ
HHHHHHHHCCCCCCCHHHCCCCCCEEECCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCC
EGVGYFQLTSRNGRRCSSAVAYLNPARSRENLRIITHAQVDRVVLDGKRATGVAYTDRSG
CCCEEEEEECCCCCCHHHHHHHCCCCCCCCCEEEEEECCCCEEEECCCCCCCEEEECCCC
TLVTVKAGKEVILCGGAINSPQLLMTSGIGEAAHLAEHGIDVVQDLHGVGKNMQDHLQAR
CEEEEECCCEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHEE
LVYKCNEPTLNDEVSSLYGQARIGLKYLMFRAGPMTMAASLATGFMRTRDDLETPDIQFH
EEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEE
VQPLSAENPGKGADKFSAFTMSVCQLRPESRGEIRLASANPRTYPRIIPNYLSTETDCRT
EEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHCCHHHCCCCCHHH
IVAGVNIARTIARHAPLTSKISEEFRPDPSLDINDYGATLDWARNNTASIYHPTGTCKMG
HHHCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCEEEECCCCEECC
PGPDAVVDARLRVHGISGLRVADCSIMPEIVSGNTNAPAIMIGEKASDLILEDAAVA
CCCCCEEEEEEEEECCCCCEEECCCCCHHHHCCCCCCCEEEECCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: choline; acceptor

Specific reaction: choline + acceptor = betaine aldehyde + reduced acceptor

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]