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The map label for this gene is nhaA

Identifier: 89056146

GI number: 89056146

Start: 3728201

End: 3729463

Strand: Direct

Name: nhaA

Synonym: Jann_3655

Alternate gene names: 89056146

Gene position: 3728201-3729463 (Clockwise)

Preceding gene: 89056145

Following gene: 89056147

Centisome position: 86.34

GC content: 60.17

Gene sequence:

>1263_bases
ATGTATCGGGTCTGGAATTTCATTACCGGATATTCACTGCTTTTGATCGGAGGCGCGATCATCGCGTTGATCTGGGCGAA
TATCGATATCAACAGCTACCATCATTTCACGGAAGTCGTGATCTGGGCCGACGCGCCTATCGGGCACCTGCACGACGACG
GCCATGGCCATGTGGTTCGCGACCTGACGCTCCATTACCTCGTGAACGATCTGCTTATGGCCTTGTTCTTCGCCATCGCC
GCAAAGGAGGTTTGGGAGGCCGTGATCCTGAAGAACGGCTCCCTGCGCGGTAAGAAGGCGGCAACGCCGTTGGTGGCCAC
GTTGGGCGGCATGGTCGGCCCAATCTCGATCTATCTCGGCATCGCTTATTTCCTGGGCTCCACCACTTTCGATGCGGTGG
CCAACGGATGGGCGATCCCCACGGCCACGGATATTGCCTTCTCCTACCTTGTGGGACGTCTGGTGTTTGGCGCGGGCCAT
CCCGCCGTGCGGTTCCTGTTGCTGTTGGCCATTGCCGACGACGCTGCGGGCCTGCTGATCCTGGCGATTTTCTACCCATC
GGGCGAATTGGCCCCGGCCTGGCTGCTTCTGTCGTTCGGGGCCGCTTTGGGCGTCTACGTGCTGGCCAATTGGCTGCCGC
GGCGCCTGGATCGCGGTGATCAACTGCGCCGTCGGTCGTCCTGGATGCGACAGAAACTGTCCTTCTGGCCCTATGCGCTG
GCCGGTTGCGCCAGTTGGTACGGCTTCATGCAATCGGGCCTGCACCCGGCGCTTGGCCTTTTGCCCATCGTGCTGACAAT
CCCCCATGCGGACCGGGCTTTCGGCATCTTTTCCGCCGCAGAAGTGCATTTGCATGACCTGCTCAACACAATGGAACACG
CGCTGAAATACCCGGTAGAGATCATCCTGGGCCTCTTTGGCCTGATGAACGCGGGCGTTGCGTTTTCGGCCATGGGAGAG
GCGACCTGGTTGGTTCTGGCGGGCCTTCTGATCGGCAAGCCTGTGGGTATTTTCCTGTTCGGATGGCTGGCGGCGAAACC
GCTTGGCTTGGGCCTGCCCCAAGGCATGCGAATGATTGATCTTGTGGTCATTGGCTGCGTGGCTGCGATTGGCTTCACCG
TCTCGCTCTTTGTGGCATCCGTCGCGTTCGAGCCCGGGCCCGTTCAGGATGCGGCCAAGATGGGAGCGTTGTTCTCGTTC
GGAGCGGCGGCGGTCTCGATCATTGTTGGCAAGCTGACACAGGTGCAGAAGCAAGAGATCTAG

Upstream 100 bases:

>100_bases
GCAATCATTGCGGGTATGGTCTGGAGTGTGATCGCCTAAGATATGGACGCCTCGCCGACCATTGGTCAGTCACCACAGGA
GCGGCAAAACGGGGACACCC

Downstream 100 bases:

>100_bases
GACGCGCGTGTCGGGGGGGCTGCCGCGCCCTCCCGACGCCGACTTGTGACACTTGGTCGAAAATGCCGTGTAATTGGTCA
GGAAAATGGACATAATACCG

Product: Na+/H+ antiporter NhaA

Products: Na (I) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Sodium/proton antiporter nhaA

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MYRVWNFITGYSLLLIGGAIIALIWANIDINSYHHFTEVVIWADAPIGHLHDDGHGHVVRDLTLHYLVNDLLMALFFAIA
AKEVWEAVILKNGSLRGKKAATPLVATLGGMVGPISIYLGIAYFLGSTTFDAVANGWAIPTATDIAFSYLVGRLVFGAGH
PAVRFLLLLAIADDAAGLLILAIFYPSGELAPAWLLLSFGAALGVYVLANWLPRRLDRGDQLRRRSSWMRQKLSFWPYAL
AGCASWYGFMQSGLHPALGLLPIVLTIPHADRAFGIFSAAEVHLHDLLNTMEHALKYPVEIILGLFGLMNAGVAFSAMGE
ATWLVLAGLLIGKPVGIFLFGWLAAKPLGLGLPQGMRMIDLVVIGCVAAIGFTVSLFVASVAFEPGPVQDAAKMGALFSF
GAAAVSIIVGKLTQVQKQEI

Sequences:

>Translated_420_residues
MYRVWNFITGYSLLLIGGAIIALIWANIDINSYHHFTEVVIWADAPIGHLHDDGHGHVVRDLTLHYLVNDLLMALFFAIA
AKEVWEAVILKNGSLRGKKAATPLVATLGGMVGPISIYLGIAYFLGSTTFDAVANGWAIPTATDIAFSYLVGRLVFGAGH
PAVRFLLLLAIADDAAGLLILAIFYPSGELAPAWLLLSFGAALGVYVLANWLPRRLDRGDQLRRRSSWMRQKLSFWPYAL
AGCASWYGFMQSGLHPALGLLPIVLTIPHADRAFGIFSAAEVHLHDLLNTMEHALKYPVEIILGLFGLMNAGVAFSAMGE
ATWLVLAGLLIGKPVGIFLFGWLAAKPLGLGLPQGMRMIDLVVIGCVAAIGFTVSLFVASVAFEPGPVQDAAKMGALFSF
GAAAVSIIVGKLTQVQKQEI
>Mature_420_residues
MYRVWNFITGYSLLLIGGAIIALIWANIDINSYHHFTEVVIWADAPIGHLHDDGHGHVVRDLTLHYLVNDLLMALFFAIA
AKEVWEAVILKNGSLRGKKAATPLVATLGGMVGPISIYLGIAYFLGSTTFDAVANGWAIPTATDIAFSYLVGRLVFGAGH
PAVRFLLLLAIADDAAGLLILAIFYPSGELAPAWLLLSFGAALGVYVLANWLPRRLDRGDQLRRRSSWMRQKLSFWPYAL
AGCASWYGFMQSGLHPALGLLPIVLTIPHADRAFGIFSAAEVHLHDLLNTMEHALKYPVEIILGLFGLMNAGVAFSAMGE
ATWLVLAGLLIGKPVGIFLFGWLAAKPLGLGLPQGMRMIDLVVIGCVAAIGFTVSLFVASVAFEPGPVQDAAKMGALFSF
GAAAVSIIVGKLTQVQKQEI

Specific function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons

COG id: COG3004

COG function: function code P; Na+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaA Na(+)/H(+) (TC 2.A.33) antiporter family

Homologues:

Organism=Escherichia coli, GI1786201, Length=350, Percent_Identity=34.5714285714286, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NHAA_JANSC (Q28L40)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_511597.1
- ProteinModelPortal:   Q28L40
- SMR:   Q28L40
- STRING:   Q28L40
- GeneID:   3936133
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_3655
- eggNOG:   COG3004
- HOGENOM:   HBG665619
- OMA:   VFGAGHP
- PhylomeDB:   Q28L40
- ProtClustDB:   CLSK828801
- BioCyc:   JSP290400:JANN_3655-MONOMER
- HAMAP:   MF_01844
- InterPro:   IPR023171
- InterPro:   IPR004670

Pfam domain/function: PF06965 Na_H_antiport_1

EC number: NA

Molecular weight: Translated: 45146; Mature: 45146

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x13272198)-; HASH(0x12e45f34)-; HASH(0x13305e54)-; HASH(0x1331b8f4)-; HASH(0x136bffc0)-; HASH(0x13adc650)-; HASH(0xca6c900)-; HASH(0x1344aa1c)-; HASH(0x128a4dc4)-; HASH(0x13adef04)-; HASH(0x13adee44)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRVWNFITGYSLLLIGGAIIALIWANIDINSYHHFTEVVIWADAPIGHLHDDGHGHVVR
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHH
DLTLHYLVNDLLMALFFAIAAKEVWEAVILKNGSLRGKKAATPLVATLGGMVGPISIYLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHHHCCHHHHHHHHHH
IAYFLGSTTFDAVANGWAIPTATDIAFSYLVGRLVFGAGHPAVRFLLLLAIADDAAGLLI
HHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEE
LAIFYPSGELAPAWLLLSFGAALGVYVLANWLPRRLDRGDQLRRRSSWMRQKLSFWPYAL
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHH
AGCASWYGFMQSGLHPALGLLPIVLTIPHADRAFGIFSAAEVHLHDLLNTMEHALKYPVE
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IILGLFGLMNAGVAFSAMGEATWLVLAGLLIGKPVGIFLFGWLAAKPLGLGLPQGMRMID
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHH
LVVIGCVAAIGFTVSLFVASVAFEPGPVQDAAKMGALFSFGAAAVSIIVGKLTQVQKQEI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MYRVWNFITGYSLLLIGGAIIALIWANIDINSYHHFTEVVIWADAPIGHLHDDGHGHVVR
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHH
DLTLHYLVNDLLMALFFAIAAKEVWEAVILKNGSLRGKKAATPLVATLGGMVGPISIYLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHHHCCHHHHHHHHHH
IAYFLGSTTFDAVANGWAIPTATDIAFSYLVGRLVFGAGHPAVRFLLLLAIADDAAGLLI
HHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEE
LAIFYPSGELAPAWLLLSFGAALGVYVLANWLPRRLDRGDQLRRRSSWMRQKLSFWPYAL
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHH
AGCASWYGFMQSGLHPALGLLPIVLTIPHADRAFGIFSAAEVHLHDLLNTMEHALKYPVE
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IILGLFGLMNAGVAFSAMGEATWLVLAGLLIGKPVGIFLFGWLAAKPLGLGLPQGMRMID
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHH
LVVIGCVAAIGFTVSLFVASVAFEPGPVQDAAKMGALFSFGAAAVSIIVGKLTQVQKQEI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + 2 Proton [Periplasm] = Na (I) [Periplasm] + 2 Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA