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The map label for this gene is dacB [H]

Identifier: 89056122

GI number: 89056122

Start: 3705325

End: 3706806

Strand: Direct

Name: dacB [H]

Synonym: Jann_3631

Alternate gene names: 89056122

Gene position: 3705325-3706806 (Clockwise)

Preceding gene: 89056120

Following gene: 89056123

Centisome position: 85.81

GC content: 65.65

Gene sequence:

>1482_bases
ATGATTCCCAGACGTCTCTTTTTGAGTGGGCTTCTTGCAAGCACCGCGCCCGGAGCATGGGCCGCCGCGCCCGAACGCTC
GCTGCGCCCGCTTGCGCGTCCGGCGGGGTTCTTGCTGCGCACCATCCCCGAAGCGGCCGAGATTATCCGCGATGCCGGTC
TTGGCGGGCGGGTTGGATTTGCCGTCGCGGACGTCGCCACCGGAGAGATCCTGGAGGTGGTCAACCCCCTCTACCCGCTG
CCACCCGCATCGGTCGCCAAAACCGTAAGTTGCGCCTACGGGCTGGATCGTTTGGGGCCGAATTATCGCTTCCGCACACG
GCTTGTATCAAACGGGGTTCTGGCCGACGGACGGCTGGACGGAGACCTGTGGCTGGTGGGCTCAGGCGATCCGCTGTTTG
ACACCGACGCGCTGGCCGCCATGGCCGATGATCTGGTCAATATGGGCCTGCGCGAGGTCACGGGGCGGTTCATGGTCGCC
ACCGGCGCGCTGCCCGAGATTTGGCAGATCGATCCCAGCCAGCTACCCCATGTGGGGTACAACCCGGCCATTTCTGCCCT
GAACCTCAACTTCAACCGGGTTCATTTTGAGTGGGAACGGGTGGGCGAGGATTATACGATTTCCATGGATGCGAGGTCTG
CGTCCTTGCGACCCGCGGTGCAGGTCGCCCGCATGCGGGTGGAGGAGCGGACAACACCGGTCTACACCTATTCCGACGCC
GAAAGCCACGACGCCTGGACGGTGGCCCGGGGCGCGTTGGGCGATGCCGGATCCCGCTGGCTTCCGGTGCGCCGACCCGC
CGCTTACATGGCCGAGGTGTTCCAGACATTGATGGGCCGCCACGGCGTGCGCCTGCCGTCCCCGACCTACGAATTGAGCG
CGCCGGAGACAGCTCTGGTGCTGGCCGAACACGTCTCGGACCCGCTGGACGAGATCCTGCGCGGGATGATGCGCTGGTCG
ACCAACCTGACGGCGGAGGTAGTTGGGCTGATGGCCACCCAGGCGACCGGGCAAAGCGCATCAAGCCTGCCCGAATCAGG
CGCCGCGATGACCGCCTGGATGCAAGAGACCCTGGGGGCCCGCAACGCCACCTTCGTCGATCATTCCGGTCTGGGGGTCG
GCAGCCGGATCAGGCCCCAGGACATGACCCACGCGCTGGTCTCGGCAGGCTCCGACAGCCTTCTGCGGGACCTGATGAAA
GACATCCCGATGCGCGACCGGGACGGGAACGTGATCCAGGGCCATCCGATAGAGGTGGTTGCCAAGACCGGCACGTTGGA
TTTCGTTTCCGCCCTGGCGGGCTTTGCACGCATGCCCTCGGGCCGCGATCTGGCATTTGCGATTTTCAGCGCTGATCTGG
ACCGGCGGGAGGCCATCCCTGACGATCAGCGGGAGCGGCCAACCGGCACGCGCACTTATAACCGGGCCGCCAAACGCATG
CAGCAGGCGCTGATCGAGCGTTGGGGCGCCGTTTACGCATGA

Upstream 100 bases:

>100_bases
CGCGGACGCGCGCAATGCACAAAACCGTGTCGCCTCGGGGGGTTGAGCGTGACACCTCCGGCGGGGTACTCTGCCCCCAG
CTGCCAAGTGATTTGAAAAC

Downstream 100 bases:

>100_bases
GATTGCCGTGATGTCCGGCCCGGTCGAGCGCTATAGCCCGCGTCGCGTCACCTATCTGGGCCTGTGGGAAGCCGGTCCCC
TGCGGATGAAGGCCTACGGT

Product: D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MIPRRLFLSGLLASTAPGAWAAAPERSLRPLARPAGFLLRTIPEAAEIIRDAGLGGRVGFAVADVATGEILEVVNPLYPL
PPASVAKTVSCAYGLDRLGPNYRFRTRLVSNGVLADGRLDGDLWLVGSGDPLFDTDALAAMADDLVNMGLREVTGRFMVA
TGALPEIWQIDPSQLPHVGYNPAISALNLNFNRVHFEWERVGEDYTISMDARSASLRPAVQVARMRVEERTTPVYTYSDA
ESHDAWTVARGALGDAGSRWLPVRRPAAYMAEVFQTLMGRHGVRLPSPTYELSAPETALVLAEHVSDPLDEILRGMMRWS
TNLTAEVVGLMATQATGQSASSLPESGAAMTAWMQETLGARNATFVDHSGLGVGSRIRPQDMTHALVSAGSDSLLRDLMK
DIPMRDRDGNVIQGHPIEVVAKTGTLDFVSALAGFARMPSGRDLAFAIFSADLDRREAIPDDQRERPTGTRTYNRAAKRM
QQALIERWGAVYA

Sequences:

>Translated_493_residues
MIPRRLFLSGLLASTAPGAWAAAPERSLRPLARPAGFLLRTIPEAAEIIRDAGLGGRVGFAVADVATGEILEVVNPLYPL
PPASVAKTVSCAYGLDRLGPNYRFRTRLVSNGVLADGRLDGDLWLVGSGDPLFDTDALAAMADDLVNMGLREVTGRFMVA
TGALPEIWQIDPSQLPHVGYNPAISALNLNFNRVHFEWERVGEDYTISMDARSASLRPAVQVARMRVEERTTPVYTYSDA
ESHDAWTVARGALGDAGSRWLPVRRPAAYMAEVFQTLMGRHGVRLPSPTYELSAPETALVLAEHVSDPLDEILRGMMRWS
TNLTAEVVGLMATQATGQSASSLPESGAAMTAWMQETLGARNATFVDHSGLGVGSRIRPQDMTHALVSAGSDSLLRDLMK
DIPMRDRDGNVIQGHPIEVVAKTGTLDFVSALAGFARMPSGRDLAFAIFSADLDRREAIPDDQRERPTGTRTYNRAAKRM
QQALIERWGAVYA
>Mature_493_residues
MIPRRLFLSGLLASTAPGAWAAAPERSLRPLARPAGFLLRTIPEAAEIIRDAGLGGRVGFAVADVATGEILEVVNPLYPL
PPASVAKTVSCAYGLDRLGPNYRFRTRLVSNGVLADGRLDGDLWLVGSGDPLFDTDALAAMADDLVNMGLREVTGRFMVA
TGALPEIWQIDPSQLPHVGYNPAISALNLNFNRVHFEWERVGEDYTISMDARSASLRPAVQVARMRVEERTTPVYTYSDA
ESHDAWTVARGALGDAGSRWLPVRRPAAYMAEVFQTLMGRHGVRLPSPTYELSAPETALVLAEHVSDPLDEILRGMMRWS
TNLTAEVVGLMATQATGQSASSLPESGAAMTAWMQETLGARNATFVDHSGLGVGSRIRPQDMTHALVSAGSDSLLRDLMK
DIPMRDRDGNVIQGHPIEVVAKTGTLDFVSALAGFARMPSGRDLAFAIFSADLDRREAIPDDQRERPTGTRTYNRAAKRM
QQALIERWGAVYA

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 53492; Mature: 53492

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRRLFLSGLLASTAPGAWAAAPERSLRPLARPAGFLLRTIPEAAEIIRDAGLGGRVGF
CCCHHHHHHHHHHHCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
AVADVATGEILEVVNPLYPLPPASVAKTVSCAYGLDRLGPNYRFRTRLVSNGVLADGRLD
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEHHHHCCCCEECCEEC
GDLWLVGSGDPLFDTDALAAMADDLVNMGLREVTGRFMVATGALPEIWQIDPSQLPHVGY
CCEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHCCCEEEECCCCCHHCCCCHHHCCCCCC
NPAISALNLNFNRVHFEWERVGEDYTISMDARSASLRPAVQVARMRVEERTTPVYTYSDA
CCCCEEEECCCEEEEEEHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCC
ESHDAWTVARGALGDAGSRWLPVRRPAAYMAEVFQTLMGRHGVRLPSPTYELSAPETALV
CCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHH
LAEHVSDPLDEILRGMMRWSTNLTAEVVGLMATQATGQSASSLPESGAAMTAWMQETLGA
HHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHCC
RNATFVDHSGLGVGSRIRPQDMTHALVSAGSDSLLRDLMKDIPMRDRDGNVIQGHPIEVV
CCCEEEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEECCCCEEEE
AKTGTLDFVSALAGFARMPSGRDLAFAIFSADLDRREAIPDDQRERPTGTRTYNRAAKRM
EECCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHCCCCCCCCCCCCCHHHHHHHHHH
QQALIERWGAVYA
HHHHHHHHCCCCC
>Mature Secondary Structure
MIPRRLFLSGLLASTAPGAWAAAPERSLRPLARPAGFLLRTIPEAAEIIRDAGLGGRVGF
CCCHHHHHHHHHHHCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
AVADVATGEILEVVNPLYPLPPASVAKTVSCAYGLDRLGPNYRFRTRLVSNGVLADGRLD
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEHHHHCCCCEECCEEC
GDLWLVGSGDPLFDTDALAAMADDLVNMGLREVTGRFMVATGALPEIWQIDPSQLPHVGY
CCEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHCCCEEEECCCCCHHCCCCHHHCCCCCC
NPAISALNLNFNRVHFEWERVGEDYTISMDARSASLRPAVQVARMRVEERTTPVYTYSDA
CCCCEEEECCCEEEEEEHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCC
ESHDAWTVARGALGDAGSRWLPVRRPAAYMAEVFQTLMGRHGVRLPSPTYELSAPETALV
CCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHH
LAEHVSDPLDEILRGMMRWSTNLTAEVVGLMATQATGQSASSLPESGAAMTAWMQETLGA
HHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHCC
RNATFVDHSGLGVGSRIRPQDMTHALVSAGSDSLLRDLMKDIPMRDRDGNVIQGHPIEVV
CCCEEEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEECCCCEEEE
AKTGTLDFVSALAGFARMPSGRDLAFAIFSADLDRREAIPDDQRERPTGTRTYNRAAKRM
EECCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHCCCCCCCCCCCCCHHHHHHHHHH
QQALIERWGAVYA
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]