Click here to switch to the map view.

The map label for this gene is pdhA [H]

Identifier: 89056048

GI number: 89056048

Start: 3620651

End: 3623038

Strand: Direct

Name: pdhA [H]

Synonym: Jann_3557

Alternate gene names: 89056048

Gene position: 3620651-3623038 (Clockwise)

Preceding gene: 89056047

Following gene: 89056057

Centisome position: 83.85

GC content: 64.11

Gene sequence:

>2388_bases
ATGGCAAGCGCGTCGGACATTCGCCACGCTCTGGAACGGAAAACCCTGTGGCTGTCGTCGTGGATGATCCACAACGCCAA
CAACATTCGCGAGAAGGGCCGGATCAAGGTCGGCGGCCATCAGGCGTCGTCGGCATCGATGAACGCCATCCTGAACGCGC
TCTACTTCGATGTGCTGCGGCCGGAAGACCGCGTGGCCGTCAAGCCCCATGCCAGCCCGGTCTTCCATGCGATCCAGTAT
CTTCTGGGCAATCAGACCCGTGATCAGTTGGAGGCCTTCCGCGCCAAGGGCGGCGCGCAACCCTATCCGTCCCGCACCAA
GGACCGGGACGATGTGGATTTCTCCACCGGGTCCGTGGGTATGGGCGTCGCCATGACCGCATTCGCGTCAATGACGCAGG
ATTATCTGGCCGGGCACGGGTGGCTGGATCGTCCCAAGGGCCGGATGATCGCCCTCGTCGGCGACGCGGAACTGGATGAA
GGCAATATCTACGAGGCGCTTCTTGAAGGGGCCAAGCACGATCTGCGCAATTGCTGGTGGATCATCGACTACAACCGCCA
ATCCCTTGACGGGGTGGTGACGCAGGGCTTGAACGCGCGGTTTCGGGATATCTTCGTGGCCTGCGGCTGGGATGTGCTGG
AGCTGAAATACGGCACCCTCATGCAGGATGCCTTCGCCCGCCCCGGTGGGACGCAATTGAAGGCGTGGATCGACGGCTGT
GACAACAACCTCTATTCCGCGCTGACCTTCAAGGGCGGGGCCGCGTGGCGGGCGCAGATGGAGGGGGATTTCACGGGCGA
CAACGAGGCTCTGGCGCTTTTGGCCGATCATAGTGACGCAGAGCTGGCGCGGTTGATGGGCAACCTCGCGGGCCATGATG
CTGCGTTGGTGGCGCAGACAATGAAGGCCATCGACCATGACCGTCCGGTCTGTTTCATCTGCTACACGGTCAAGGGCCAC
GGGCTGCCGCTGGCGGGCCACAAGGACAACCATGCCGGGATCATGACCAGTGCCCAGATCGAGGATCTGCGCGACACGAT
GGGCATAGCCGTCGGAGCGGAATGGGAGGCCTTTGCAGGCATCGACATCCCCGAGGCGGAGGTCAGGGATTTCCTGGACA
CCGTACCGTTCAACGCCGAGGGGCAACGCCGTCTGAACGCGAACCCCGTGCCCACCTACGACCGCGTTGATCCACGGATC
AAAGGCGTCACCTCCACCCAGGAGGCGTTTGGCAAGATCCTCGCGGCCATCGCCTCGGACGCGCCGGATCTGGCGGAGCG
CATTGTCACGACCTCTCCGGATGTCTCCGTGTCCACCAACCTCGGGCCGTGGATCAACAAGACCGGCCTTTTCGCCAAAA
CGGATCAGGCCGATGTCTTCCGCGATCAATCCGTGCCGTCGGTGCAGAAATGGCGACGCCACGGGGGCGGGCGGCATATG
GAGCTTGGGATTGCCGAGAACAACCTGTTCACCTTCCTCGGCGCGCTTGGCCTGTCGCATTCCCTGTTCGGTCAACGGCT
GCTGCCGGTGGGCACGGTCTACGATCCGTTCATCTGCCGTGGCCTCGATGCGCTGAACTACGCCTGCTACCAGGATGCGC
GGTTCATGCTGGCGGCAACGCCATCGGGCGTGTCGCTCAGCCATGAAGGCGGCGCGCACCAATCTATTTCGACACCGCTG
ATCGGCATGGCGCAGGATGGTCTGGCGAGCTTTGAGCCCGCGTTTGCCGACGAGTTGGCGACGATCATGCGGTGGGGGTT
CGACTACATGCAACGCGACGGGTCCGGTGATCCGTCCGAGGATAACTGGCTGCGCGACGAGACCGGCGGTTCTGTCTATC
TGCGGCTGTCGACCGTGCCGCTTGAGCAGATCGACCGCACCACGGACGCGGCGTTTGAGACGGGCGTGCTTGATGGTGGT
TACTGGCTACGCCGACCGGAGCCCGGTACCCGTGTCGCCATTGCCTATACGGGCGTCGTCGCGCCGGAAGCCATCCGCGC
GGCGGGCCTTCTGTCCGAGACCATGCGCGGCATCGGCGTGTTGGCCATCACGTCCGCCGACCGTCTGAACGCCGGGTGGC
AGGCGGCGGAGCGGGCCCGCCAACGGGGCCAGACGGGCGTCAGGTCAACGATTGAAACCTTGCTGGCCGATCTGCCGTCT
GATGCGGGCCTCGTGACCGTCGCCGACGCCCACCCTGTGACCCTGTCTTGGCTGGGTGGGGTCGAGGGTCACCGGGTGAA
ATCCCTTGGAGTGGAGCATTTCGGACAGGCCGGAACCGTGGACGACATCTACCGCCTGCACGGAATTGATGCCAACGCCA
TTCAACACGCGGCCATGGCGCTGATGGGCGGACAGAGCGCGCGCTACGCGCAACCGCTACCCGGCTGA

Upstream 100 bases:

>100_bases
GCTGGCGATACTTTGGGCTTACGGATCATGACTGAGAATGTGCACCTTTTCGCTGAAGGAGGTGCGGCCCTGCCCGCCTC
GTGCCGTCGGGCAGCCTGAC

Downstream 100 bases:

>100_bases
TGCCAGCCCGACCGCTCAACCTGATGCGTAAGCCGGGATGAGACGCACCGCACCGTAAGGCGGGGTTTCGCAAAACCTCG
CTTTTTAGACATGTCTTATT

Product: transketolase protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 795; Mature: 794

Protein sequence:

>795_residues
MASASDIRHALERKTLWLSSWMIHNANNIREKGRIKVGGHQASSASMNAILNALYFDVLRPEDRVAVKPHASPVFHAIQY
LLGNQTRDQLEAFRAKGGAQPYPSRTKDRDDVDFSTGSVGMGVAMTAFASMTQDYLAGHGWLDRPKGRMIALVGDAELDE
GNIYEALLEGAKHDLRNCWWIIDYNRQSLDGVVTQGLNARFRDIFVACGWDVLELKYGTLMQDAFARPGGTQLKAWIDGC
DNNLYSALTFKGGAAWRAQMEGDFTGDNEALALLADHSDAELARLMGNLAGHDAALVAQTMKAIDHDRPVCFICYTVKGH
GLPLAGHKDNHAGIMTSAQIEDLRDTMGIAVGAEWEAFAGIDIPEAEVRDFLDTVPFNAEGQRRLNANPVPTYDRVDPRI
KGVTSTQEAFGKILAAIASDAPDLAERIVTTSPDVSVSTNLGPWINKTGLFAKTDQADVFRDQSVPSVQKWRRHGGGRHM
ELGIAENNLFTFLGALGLSHSLFGQRLLPVGTVYDPFICRGLDALNYACYQDARFMLAATPSGVSLSHEGGAHQSISTPL
IGMAQDGLASFEPAFADELATIMRWGFDYMQRDGSGDPSEDNWLRDETGGSVYLRLSTVPLEQIDRTTDAAFETGVLDGG
YWLRRPEPGTRVAIAYTGVVAPEAIRAAGLLSETMRGIGVLAITSADRLNAGWQAAERARQRGQTGVRSTIETLLADLPS
DAGLVTVADAHPVTLSWLGGVEGHRVKSLGVEHFGQAGTVDDIYRLHGIDANAIQHAAMALMGGQSARYAQPLPG

Sequences:

>Translated_795_residues
MASASDIRHALERKTLWLSSWMIHNANNIREKGRIKVGGHQASSASMNAILNALYFDVLRPEDRVAVKPHASPVFHAIQY
LLGNQTRDQLEAFRAKGGAQPYPSRTKDRDDVDFSTGSVGMGVAMTAFASMTQDYLAGHGWLDRPKGRMIALVGDAELDE
GNIYEALLEGAKHDLRNCWWIIDYNRQSLDGVVTQGLNARFRDIFVACGWDVLELKYGTLMQDAFARPGGTQLKAWIDGC
DNNLYSALTFKGGAAWRAQMEGDFTGDNEALALLADHSDAELARLMGNLAGHDAALVAQTMKAIDHDRPVCFICYTVKGH
GLPLAGHKDNHAGIMTSAQIEDLRDTMGIAVGAEWEAFAGIDIPEAEVRDFLDTVPFNAEGQRRLNANPVPTYDRVDPRI
KGVTSTQEAFGKILAAIASDAPDLAERIVTTSPDVSVSTNLGPWINKTGLFAKTDQADVFRDQSVPSVQKWRRHGGGRHM
ELGIAENNLFTFLGALGLSHSLFGQRLLPVGTVYDPFICRGLDALNYACYQDARFMLAATPSGVSLSHEGGAHQSISTPL
IGMAQDGLASFEPAFADELATIMRWGFDYMQRDGSGDPSEDNWLRDETGGSVYLRLSTVPLEQIDRTTDAAFETGVLDGG
YWLRRPEPGTRVAIAYTGVVAPEAIRAAGLLSETMRGIGVLAITSADRLNAGWQAAERARQRGQTGVRSTIETLLADLPS
DAGLVTVADAHPVTLSWLGGVEGHRVKSLGVEHFGQAGTVDDIYRLHGIDANAIQHAAMALMGGQSARYAQPLPG
>Mature_794_residues
ASASDIRHALERKTLWLSSWMIHNANNIREKGRIKVGGHQASSASMNAILNALYFDVLRPEDRVAVKPHASPVFHAIQYL
LGNQTRDQLEAFRAKGGAQPYPSRTKDRDDVDFSTGSVGMGVAMTAFASMTQDYLAGHGWLDRPKGRMIALVGDAELDEG
NIYEALLEGAKHDLRNCWWIIDYNRQSLDGVVTQGLNARFRDIFVACGWDVLELKYGTLMQDAFARPGGTQLKAWIDGCD
NNLYSALTFKGGAAWRAQMEGDFTGDNEALALLADHSDAELARLMGNLAGHDAALVAQTMKAIDHDRPVCFICYTVKGHG
LPLAGHKDNHAGIMTSAQIEDLRDTMGIAVGAEWEAFAGIDIPEAEVRDFLDTVPFNAEGQRRLNANPVPTYDRVDPRIK
GVTSTQEAFGKILAAIASDAPDLAERIVTTSPDVSVSTNLGPWINKTGLFAKTDQADVFRDQSVPSVQKWRRHGGGRHME
LGIAENNLFTFLGALGLSHSLFGQRLLPVGTVYDPFICRGLDALNYACYQDARFMLAATPSGVSLSHEGGAHQSISTPLI
GMAQDGLASFEPAFADELATIMRWGFDYMQRDGSGDPSEDNWLRDETGGSVYLRLSTVPLEQIDRTTDAAFETGVLDGGY
WLRRPEPGTRVAIAYTGVVAPEAIRAAGLLSETMRGIGVLAITSADRLNAGWQAAERARQRGQTGVRSTIETLLADLPSD
AGLVTVADAHPVTLSWLGGVEGHRVKSLGVEHFGQAGTVDDIYRLHGIDANAIQHAAMALMGGQSARYAQPLPG

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG2609

COG function: function code C; Pyruvate dehydrogenase complex, dehydrogenase (E1) component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786304, Length=788, Percent_Identity=22.9695431472081, Blast_Score=128, Evalue=2e-30,

Paralogues:

None

Copy number: 1140 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 400 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004660
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005474 [H]

Pfam domain/function: PF00456 Transketolase_N [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 86144; Mature: 86013

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASASDIRHALERKTLWLSSWMIHNANNIREKGRIKVGGHQASSASMNAILNALYFDVLR
CCCHHHHHHHHHHHHHHHHHHHEECCCCHHHCCCEEECCCCCCHHHHHHHHHHHHHHHCC
PEDRVAVKPHASPVFHAIQYLLGNQTRDQLEAFRAKGGAQPYPSRTKDRDDVDFSTGSVG
CCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
MGVAMTAFASMTQDYLAGHGWLDRPKGRMIALVGDAELDEGNIYEALLEGAKHDLRNCWW
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEE
IIDYNRQSLDGVVTQGLNARFRDIFVACGWDVLELKYGTLMQDAFARPGGTQLKAWIDGC
EEECCCCHHHHHHHCCCCCHHHEEEEECCCEEEEEHHHHHHHHHHCCCCCCEEHHHHCCC
DNNLYSALTFKGGAAWRAQMEGDFTGDNEALALLADHSDAELARLMGNLAGHDAALVAQT
CCCCEEEEEECCCCEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHH
MKAIDHDRPVCFICYTVKGHGLPLAGHKDNHAGIMTSAQIEDLRDTMGIAVGAEWEAFAG
HHHHCCCCCEEEEEEEECCCCEECCCCCCCCCCEEEHHHHHHHHHHHCEEECCCCCCCCC
IDIPEAEVRDFLDTVPFNAEGQRRLNANPVPTYDRVDPRIKGVTSTQEAFGKILAAIASD
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
APDLAERIVTTSPDVSVSTNLGPWINKTGLFAKTDQADVFRDQSVPSVQKWRRHGGGRHM
CHHHHHHHHCCCCCCEEECCCCCCCCCCCCEEECCCHHHHHCCCCCHHHHHHHCCCCCEE
ELGIAENNLFTFLGALGLSHSLFGQRLLPVGTVYDPFICRGLDALNYACYQDARFMLAAT
EEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCHHHHHHHHHHCCEEEEEEC
PSGVSLSHEGGAHQSISTPLIGMAQDGLASFEPAFADELATIMRWGFDYMQRDGSGDPSE
CCCCEECCCCCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHCHHHHHCCCCCCCCC
DNWLRDETGGSVYLRLSTVPLEQIDRTTDAAFETGVLDGGYWLRRPEPGTRVAIAYTGVV
CCCCCCCCCCEEEEEEECCCHHHHHCHHHHHHHCCCCCCCEEEECCCCCCEEEEEEECCC
APEAIRAAGLLSETMRGIGVLAITSADRLNAGWQAAERARQRGQTGVRSTIETLLADLPS
CHHHHHHHHHHHHHHHCCEEEEEECCHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCC
DAGLVTVADAHPVTLSWLGGVEGHRVKSLGVEHFGQAGTVDDIYRLHGIDANAIQHAAMA
CCCEEEEECCCCEEEEECCCCCCCHHHHHCHHHCCCCCCHHHHHHHCCCCHHHHHHHHHH
LMGGQSARYAQPLPG
HHCCCCCCCCCCCCC
>Mature Secondary Structure 
ASASDIRHALERKTLWLSSWMIHNANNIREKGRIKVGGHQASSASMNAILNALYFDVLR
CCHHHHHHHHHHHHHHHHHHHEECCCCHHHCCCEEECCCCCCHHHHHHHHHHHHHHHCC
PEDRVAVKPHASPVFHAIQYLLGNQTRDQLEAFRAKGGAQPYPSRTKDRDDVDFSTGSVG
CCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
MGVAMTAFASMTQDYLAGHGWLDRPKGRMIALVGDAELDEGNIYEALLEGAKHDLRNCWW
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEE
IIDYNRQSLDGVVTQGLNARFRDIFVACGWDVLELKYGTLMQDAFARPGGTQLKAWIDGC
EEECCCCHHHHHHHCCCCCHHHEEEEECCCEEEEEHHHHHHHHHHCCCCCCEEHHHHCCC
DNNLYSALTFKGGAAWRAQMEGDFTGDNEALALLADHSDAELARLMGNLAGHDAALVAQT
CCCCEEEEEECCCCEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHH
MKAIDHDRPVCFICYTVKGHGLPLAGHKDNHAGIMTSAQIEDLRDTMGIAVGAEWEAFAG
HHHHCCCCCEEEEEEEECCCCEECCCCCCCCCCEEEHHHHHHHHHHHCEEECCCCCCCCC
IDIPEAEVRDFLDTVPFNAEGQRRLNANPVPTYDRVDPRIKGVTSTQEAFGKILAAIASD
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
APDLAERIVTTSPDVSVSTNLGPWINKTGLFAKTDQADVFRDQSVPSVQKWRRHGGGRHM
CHHHHHHHHCCCCCCEEECCCCCCCCCCCCEEECCCHHHHHCCCCCHHHHHHHCCCCCEE
ELGIAENNLFTFLGALGLSHSLFGQRLLPVGTVYDPFICRGLDALNYACYQDARFMLAAT
EEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCHHHHHHHHHHCCEEEEEEC
PSGVSLSHEGGAHQSISTPLIGMAQDGLASFEPAFADELATIMRWGFDYMQRDGSGDPSE
CCCCEECCCCCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHCHHHHHCCCCCCCCC
DNWLRDETGGSVYLRLSTVPLEQIDRTTDAAFETGVLDGGYWLRRPEPGTRVAIAYTGVV
CCCCCCCCCCEEEEEEECCCHHHHHCHHHHHHHCCCCCCCEEEECCCCCCEEEEEEECCC
APEAIRAAGLLSETMRGIGVLAITSADRLNAGWQAAERARQRGQTGVRSTIETLLADLPS
CHHHHHHHHHHHHHHHCCEEEEEECCHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCC
DAGLVTVADAHPVTLSWLGGVEGHRVKSLGVEHFGQAGTVDDIYRLHGIDANAIQHAAMA
CCCEEEEECCCCEEEEECCCCCCCHHHHHCHHHCCCCCCHHHHHHHCCCCHHHHHHHHHH
LMGGQSARYAQPLPG
HHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8021225 [H]