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The map label for this gene is nadE

Identifier: 89055837

GI number: 89055837

Start: 3398207

End: 3399865

Strand: Direct

Name: nadE

Synonym: Jann_3346

Alternate gene names: 89055837

Gene position: 3398207-3399865 (Clockwise)

Preceding gene: 89055834

Following gene: 89055839

Centisome position: 78.7

GC content: 63.35

Gene sequence:

>1659_bases
ATGGCCGATACGTTTCGCCTGACGCTGGCACAGCTGAACCCAACCCTTGGCGACCTGGGCGGCAACGCAAAACTGGTGCG
CGAAGCCTTTGCCGAGGCCAAGGCCGAGGACAGCGATTTCCTGGCCTTCCCCGAGATGTTCATCACCGGCTACCAACTGC
TGGATCTGGTGATGAAACAGGCCTTCGCCGAAGATGTGCAGCGGGTGATCGCGGACCTGGCCGCGGAATTGCACGACGGC
CCCGCCTTCGGGATCGGCGGGCCGATGTGGGGCGGCGACAAGCCCTACAACGCCTATTACATCTGCGAAGGTGGCAAGAT
CGCGGCCACGATCCTGAAGCACCATCTGCCAAACTACGCGGTCTTTGATGAGGTGCGTTATTATCACAGCGCCGACCCGC
AAGGCCCGGTGAACATCAACGGTGTGCGCATCGGCTTTCCGATTTGCGAGGATGCCTGGTTTGAGGATGTCTGCGAGACG
CTGGAAGAATCCGGCGCGGAGATCCTGATTTCCCCCAACGGATCGCCCTATCATCGCGGCAAGATGGAGCTGCGGCAATC
CGTGGTCGTGTCCCGTGTGGTCGAGACGGGCCTGCCGATGGCCTATCTGAACCTTCTGGGCGGGCAGGACGATCAGGTTT
TCGACGGCGGATCCTTTGTGCTGAACCGGGGCGGCGCGCTGGCCGTGCAGATGCCGCAATTCGAGGCCGGTCTGGAACAG
GTCGATTTCGCCCGTGGCGATGACGGCTGGGTGGCGAAAGACGGCGTGAAAGCCCGTCTCCCCGAGGCGTATGAGGCTGA
CTACCGCGTCATGGTCATGGCTTTGGGCGATTATCTGCGCAAGACGGGGTTCACGTCGGTCGTTCTAGGCCTGTCGGGCG
GGATCGACTCCGCCATCGTGGCCTGCATCGCCGCGGACGCCATCGGGCCCGAGAACGTCCACTGCGTGATGCTGCCCTCT
CGCTTCACGTCCGAGGTATCGCTGGACGACGCCCGCGATGTGGCCGCGCGGTTGGGGTGCCGCCTGGACACCATCCCGAT
CACCCCCGCGCGCGAGGCGGTGACGGCGTCCCTTGCGCCACTTTTCGAGGGGCTGGAAGAGGATGTGACGGAGGAGAATA
TCCAGTCCCGCCTGCGCGGTGTCATGCTGATGGCGCTGAGCAACAAGTTCGGCTCCATGCTGTTGACCACGGGCAACAAA
TCCGAGGTCGCGGTGGGCTATGCCACGATCTATGGCGACATGGCCGGGGGCTATAACCCGATCAAGGACCTCTACAAGAC
CCGCGTGTTCGAGACCTGCCGCTGGCGCAACCGCGAGCACCGCGACTGGATGCTGGCCCCGGCGGGCGAAATCATCCCGC
CCCGGGTTATCGACAAGCCCCCCACCGCCGAATTGCGAGAGGACCAGAAGGACGAGGACAGCCTGCCCCCTTATGCCGAG
TTGGACGTGATGCTGGAGATGCTGATCGACAAGGACCTGTCGGTCGCCGAAGTGGTGGCGGCAGGGTACGATCCTGAGTG
GGTTCGGAAGATAGAGCGACTGATTTACCTGAGCGAATACAAACGGTTCCAGGCCGCCCCCGGCGCGCGCCTCACACAAA
AGGCATTCTGGCTGGACCGCCGATACCCGGTGGCAAATCGCTGGCGGGATGGCGGCTGA

Upstream 100 bases:

>100_bases
GCGTACACGCAACGTCTTTAACCCCTGGGCGGCGGTGCTTGGCGCTTGGACGCGCGCGCCAACCTGATATGACGGGCGGG
ATACAGAATTTGGAGTCCCC

Downstream 100 bases:

>100_bases
GGCGGCGGGACATGGCAGAACATAACGACTATGCCTTTTCCGCAAACCCGTGGCTTGAAACATGTAATTGCAACCACGAT
CTTCTCACCAAGCGACCAAA

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MADTFRLTLAQLNPTLGDLGGNAKLVREAFAEAKAEDSDFLAFPEMFITGYQLLDLVMKQAFAEDVQRVIADLAAELHDG
PAFGIGGPMWGGDKPYNAYYICEGGKIAATILKHHLPNYAVFDEVRYYHSADPQGPVNINGVRIGFPICEDAWFEDVCET
LEESGAEILISPNGSPYHRGKMELRQSVVVSRVVETGLPMAYLNLLGGQDDQVFDGGSFVLNRGGALAVQMPQFEAGLEQ
VDFARGDDGWVAKDGVKARLPEAYEADYRVMVMALGDYLRKTGFTSVVLGLSGGIDSAIVACIAADAIGPENVHCVMLPS
RFTSEVSLDDARDVAARLGCRLDTIPITPAREAVTASLAPLFEGLEEDVTEENIQSRLRGVMLMALSNKFGSMLLTTGNK
SEVAVGYATIYGDMAGGYNPIKDLYKTRVFETCRWRNREHRDWMLAPAGEIIPPRVIDKPPTAELREDQKDEDSLPPYAE
LDVMLEMLIDKDLSVAEVVAAGYDPEWVRKIERLIYLSEYKRFQAAPGARLTQKAFWLDRRYPVANRWRDGG

Sequences:

>Translated_552_residues
MADTFRLTLAQLNPTLGDLGGNAKLVREAFAEAKAEDSDFLAFPEMFITGYQLLDLVMKQAFAEDVQRVIADLAAELHDG
PAFGIGGPMWGGDKPYNAYYICEGGKIAATILKHHLPNYAVFDEVRYYHSADPQGPVNINGVRIGFPICEDAWFEDVCET
LEESGAEILISPNGSPYHRGKMELRQSVVVSRVVETGLPMAYLNLLGGQDDQVFDGGSFVLNRGGALAVQMPQFEAGLEQ
VDFARGDDGWVAKDGVKARLPEAYEADYRVMVMALGDYLRKTGFTSVVLGLSGGIDSAIVACIAADAIGPENVHCVMLPS
RFTSEVSLDDARDVAARLGCRLDTIPITPAREAVTASLAPLFEGLEEDVTEENIQSRLRGVMLMALSNKFGSMLLTTGNK
SEVAVGYATIYGDMAGGYNPIKDLYKTRVFETCRWRNREHRDWMLAPAGEIIPPRVIDKPPTAELREDQKDEDSLPPYAE
LDVMLEMLIDKDLSVAEVVAAGYDPEWVRKIERLIYLSEYKRFQAAPGARLTQKAFWLDRRYPVANRWRDGG
>Mature_551_residues
ADTFRLTLAQLNPTLGDLGGNAKLVREAFAEAKAEDSDFLAFPEMFITGYQLLDLVMKQAFAEDVQRVIADLAAELHDGP
AFGIGGPMWGGDKPYNAYYICEGGKIAATILKHHLPNYAVFDEVRYYHSADPQGPVNINGVRIGFPICEDAWFEDVCETL
EESGAEILISPNGSPYHRGKMELRQSVVVSRVVETGLPMAYLNLLGGQDDQVFDGGSFVLNRGGALAVQMPQFEAGLEQV
DFARGDDGWVAKDGVKARLPEAYEADYRVMVMALGDYLRKTGFTSVVLGLSGGIDSAIVACIAADAIGPENVHCVMLPSR
FTSEVSLDDARDVAARLGCRLDTIPITPAREAVTASLAPLFEGLEEDVTEENIQSRLRGVMLMALSNKFGSMLLTTGNKS
EVAVGYATIYGDMAGGYNPIKDLYKTRVFETCRWRNREHRDWMLAPAGEIIPPRVIDKPPTAELREDQKDEDSLPPYAEL
DVMLEMLIDKDLSVAEVVAAGYDPEWVRKIERLIYLSEYKRFQAAPGARLTQKAFWLDRRYPVANRWRDGG

Specific function: It is not known if this NAD synthase uses NH(3) or glutamine as the N donor [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 60888; Mature: 60757

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTFRLTLAQLNPTLGDLGGNAKLVREAFAEAKAEDSDFLAFPEMFITGYQLLDLVMKQ
CCCCCEEEHHHCCCHHHHCCCCHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHH
AFAEDVQRVIADLAAELHDGPAFGIGGPMWGGDKPYNAYYICEGGKIAATILKHHLPNYA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCHH
VFDEVRYYHSADPQGPVNINGVRIGFPICEDAWFEDVCETLEESGAEILISPNGSPYHRG
HHHHHHHHHCCCCCCCEEECEEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCH
KMELRQSVVVSRVVETGLPMAYLNLLGGQDDQVFDGGSFVLNRGGALAVQMPQFEAGLEQ
HHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEECCCCEEEECCCEEEEECCHHHHCHHH
VDFARGDDGWVAKDGVKARLPEAYEADYRVMVMALGDYLRKTGFTSVVLGLSGGIDSAIV
HHCCCCCCCCEECCCCHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHEEEECCCCHHHHHH
ACIAADAIGPENVHCVMLPSRFTSEVSLDDARDVAARLGCRLDTIPITPAREAVTASLAP
HHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCEECCCCCCCHHHHHHHHHH
LFEGLEEDVTEENIQSRLRGVMLMALSNKFGSMLLTTGNKSEVAVGYATIYGDMAGGYNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEEEEECCCCCCCH
IKDLYKTRVFETCRWRNREHRDWMLAPAGEIIPPRVIDKPPTAELREDQKDEDSLPPYAE
HHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHH
LDVMLEMLIDKDLSVAEVVAAGYDPEWVRKIERLIYLSEYKRFQAAPGARLTQKAFWLDR
HHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
RYPVANRWRDGG
CCCHHCCCCCCC
>Mature Secondary Structure 
ADTFRLTLAQLNPTLGDLGGNAKLVREAFAEAKAEDSDFLAFPEMFITGYQLLDLVMKQ
CCCCEEEHHHCCCHHHHCCCCHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHH
AFAEDVQRVIADLAAELHDGPAFGIGGPMWGGDKPYNAYYICEGGKIAATILKHHLPNYA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCHH
VFDEVRYYHSADPQGPVNINGVRIGFPICEDAWFEDVCETLEESGAEILISPNGSPYHRG
HHHHHHHHHCCCCCCCEEECEEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCH
KMELRQSVVVSRVVETGLPMAYLNLLGGQDDQVFDGGSFVLNRGGALAVQMPQFEAGLEQ
HHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEECCCCEEEECCCEEEEECCHHHHCHHH
VDFARGDDGWVAKDGVKARLPEAYEADYRVMVMALGDYLRKTGFTSVVLGLSGGIDSAIV
HHCCCCCCCCEECCCCHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHEEEECCCCHHHHHH
ACIAADAIGPENVHCVMLPSRFTSEVSLDDARDVAARLGCRLDTIPITPAREAVTASLAP
HHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCEECCCCCCCHHHHHHHHHH
LFEGLEEDVTEENIQSRLRGVMLMALSNKFGSMLLTTGNKSEVAVGYATIYGDMAGGYNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEEEEECCCCCCCH
IKDLYKTRVFETCRWRNREHRDWMLAPAGEIIPPRVIDKPPTAELREDQKDEDSLPPYAE
HHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHH
LDVMLEMLIDKDLSVAEVVAAGYDPEWVRKIERLIYLSEYKRFQAAPGARLTQKAFWLDR
HHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
RYPVANRWRDGG
CCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8431308; 8195100 [H]