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The map label for this gene is cobS [H]

Identifier: 89055797

GI number: 89055797

Start: 3361434

End: 3362255

Strand: Direct

Name: cobS [H]

Synonym: Jann_3306

Alternate gene names: 89055797

Gene position: 3361434-3362255 (Clockwise)

Preceding gene: 89055795

Following gene: 89055804

Centisome position: 77.85

GC content: 68.13

Gene sequence:

>822_bases
ATGTACCGGATTACGTCGCGTGCGCCCTGCGGCAGAAAGGTCTTTTTCATGCCTGAAACCGACACCACCCCATCGCGGCC
GCGCCCACAGCTTCGCGACCTGATCCGCGCGGTTCATTTGCTGACCCGCCTGCCCCTGCCCGGCGGCGACGCAACCCGTG
GGGCCGCCGCAGCCTGGGCCTGGCCCCTGGTGGGCCTGATCGTCGGTCTGGGACAGGTGGCGGCTGGCCTTTTGGCCCTG
TGGCTTGGCGTGTCGGAAGCTGTGGCCGCAGGGGTGGCGATTGCGGCGGGGCTGTTGATCACCGGCGGATTGCATGAGGA
TGGACTGGCCGATTGCGCCGACGGGTTCTGGGGCGGAATGACCCCCGCGCGCCGGTTGGAAATCCTGAAAGACAGCCGCA
TCGGCGCCTACGGCGTCCTTGCGTTGATCGTGACCATCGGCTTTCGGTGGGCCTTGTTCGCGTCCCTCCTCGCAATCGCT
CCGCTCACGCTCCTCGCAGCCGCGATGATCAGCCGGGCGGGGATCGCGGTGGTGATGGCGCGCCTGCCTTTTGCGCGTGA
GGGTGGCTTGGCCGCGCATGTGGGGCGCCCTCCGGCCTGGGCGGTCGGTCTGGGCGGTATCATTGCGGGATTGGGGGCTA
TCGTGTTTGCCGGGATCGGCGCGGGGCTCGCGGCGCTGGGTGTCGCAACCGTCGCGATCCTGATCGTCGCCGCCCTCGCA
CGCGCCAAAATTGGCGGGCAAACCGGGGACGTTCTGGGTGCGACGCAGGTGGTGGCAGAGCTTGCTGCGCTGGCCACCTG
CGCCGCGTTTTTATCTACTTAG

Upstream 100 bases:

>100_bases
GCAGCGGCAAGCGCTGTTTGGAACTCTACGAGGGTGGTGGGTGTGGTCATGGATCTGCCTCTCTTGCGTGCCCGGACTTG
TGTGACCCGGTTGGGTTGAT

Downstream 100 bases:

>100_bases
CAAGGCGCGACGTAGTAGGTGCCATTGCCGTTGGAATAGGCGCATTGGCCGGTCTGCGTGTTGCGGGCAACCTGTGTGCC
GATGGCCGCACCGGCAAGCG

Product: cobalamin-5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MYRITSRAPCGRKVFFMPETDTTPSRPRPQLRDLIRAVHLLTRLPLPGGDATRGAAAAWAWPLVGLIVGLGQVAAGLLAL
WLGVSEAVAAGVAIAAGLLITGGLHEDGLADCADGFWGGMTPARRLEILKDSRIGAYGVLALIVTIGFRWALFASLLAIA
PLTLLAAAMISRAGIAVVMARLPFAREGGLAAHVGRPPAWAVGLGGIIAGLGAIVFAGIGAGLAALGVATVAILIVAALA
RAKIGGQTGDVLGATQVVAELAALATCAAFLST

Sequences:

>Translated_273_residues
MYRITSRAPCGRKVFFMPETDTTPSRPRPQLRDLIRAVHLLTRLPLPGGDATRGAAAAWAWPLVGLIVGLGQVAAGLLAL
WLGVSEAVAAGVAIAAGLLITGGLHEDGLADCADGFWGGMTPARRLEILKDSRIGAYGVLALIVTIGFRWALFASLLAIA
PLTLLAAAMISRAGIAVVMARLPFAREGGLAAHVGRPPAWAVGLGGIIAGLGAIVFAGIGAGLAALGVATVAILIVAALA
RAKIGGQTGDVLGATQVVAELAALATCAAFLST
>Mature_273_residues
MYRITSRAPCGRKVFFMPETDTTPSRPRPQLRDLIRAVHLLTRLPLPGGDATRGAAAAWAWPLVGLIVGLGQVAAGLLAL
WLGVSEAVAAGVAIAAGLLITGGLHEDGLADCADGFWGGMTPARRLEILKDSRIGAYGVLALIVTIGFRWALFASLLAIA
PLTLLAAAMISRAGIAVVMARLPFAREGGLAAHVGRPPAWAVGLGGIIAGLGAIVFAGIGAGLAALGVATVAILIVAALA
RAKIGGQTGDVLGATQVVAELAALATCAAFLST

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=245, Percent_Identity=35.5102040816327, Blast_Score=102, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 27545; Mature: 27545

Theoretical pI: Translated: 10.64; Mature: 10.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRITSRAPCGRKVFFMPETDTTPSRPRPQLRDLIRAVHLLTRLPLPGGDATRGAAAAWA
CCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
WPLVGLIVGLGQVAAGLLALWLGVSEAVAAGVAIAAGLLITGGLHEDGLADCADGFWGGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCC
TPARRLEILKDSRIGAYGVLALIVTIGFRWALFASLLAIAPLTLLAAAMISRAGIAVVMA
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLPFAREGGLAAHVGRPPAWAVGLGGIIAGLGAIVFAGIGAGLAALGVATVAILIVAALA
HCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAKIGGQTGDVLGATQVVAELAALATCAAFLST
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYRITSRAPCGRKVFFMPETDTTPSRPRPQLRDLIRAVHLLTRLPLPGGDATRGAAAAWA
CCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
WPLVGLIVGLGQVAAGLLALWLGVSEAVAAGVAIAAGLLITGGLHEDGLADCADGFWGGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCC
TPARRLEILKDSRIGAYGVLALIVTIGFRWALFASLLAIAPLTLLAAAMISRAGIAVVMA
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLPFAREGGLAAHVGRPPAWAVGLGGIIAGLGAIVFAGIGAGLAALGVATVAILIVAALA
HCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAKIGGQTGDVLGATQVVAELAALATCAAFLST
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]