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The map label for this gene is yeiR [C]

Identifier: 89055742

GI number: 89055742

Start: 3301568

End: 3302440

Strand: Direct

Name: yeiR [C]

Synonym: Jann_3251

Alternate gene names: 89055742

Gene position: 3301568-3302440 (Clockwise)

Preceding gene: 89055741

Following gene: 89055745

Centisome position: 76.46

GC content: 63.0

Gene sequence:

>873_bases
ATGATGCCCCTGCCGTTGACAGTGGTGGCGGGATATTTGGGTGCCGGAAAGACCACGTTTATCAATCGCTTGCTGGCAGG
AGACCACAGGCTGCGCCTGATGGTACTGGTCAACGATTTCGGCGCGATCAACATCGACGCGGGCCTGATCGCATTGGCAG
ACGGTGACACCATCGCGCTGACCAACGGCTGCGTTTGCTGCACCATGGGTGCGGACTTGTTCATGGCATTGGGCGACGCG
CTGGATCGCCGACCGCGCCCCGACCACCTGATCGTAGAAGCCAGCGGCGTGGCTGATCCTGCACGCATCGCGAACGCGGC
CCTGGCCGAGCCGGATCTGGCTTACGGGGGGATCGTGACAATCGTGGACGGGCAGCAAATCGACGGGCTATGCGCGGATC
CGATGATCGGAGCGCAGGTCCGCGATCAGATCGCCTGCGCGGATCTGGTGGTGGTCAGCAAGGTCTGCGAGCTATCCGCC
GTATTGGCCGCCACTCTCACCACCCTATCGGGCGCGAGTGTTCTCTTGTCAGCGGACGTGACAGACCCCGCGACGTTGTT
ATGCGCGGCACCGAAACCGACGCCTGCCTCAGCGCCCCATCCGGATTACCAAAGTTGGTCCTACACCGGAGACGCGACGC
TGCACCGTGAGGTCCTTTCCCAGTCTGTCGCCAAGCGCCCTCAGGCCCTCTTTCGCCTCAAGGGACACGTCATGGACCCG
AACGGTGGCGCGTGGGAGGTTCACGCGGTGGGGCGGCACGTCTCGGTCACCCAGGCCGCGCCGCAATCGCACACGCAGTT
GGTCGCCATAGGACTGAAATCCCTTGTAAAAAAAGATGACGTCGACGCGTGGTGGCAATCCGCAACGGCTTAG

Upstream 100 bases:

>100_bases
ATTTCGCGGTGTTGGGTGCAGATCCAAGCGCCGTTGACCCCATGGATCTGAAAGATGTCCCGGTTCTGGGCACCGTTTCG
GGCGGGCGGGCGCATCTGCT

Downstream 100 bases:

>100_bases
TCGTCGGCCCAGGGTCCGTGCTGATCTTTGTCGCCCGTGTCCAGACGCACAAACCCATGCAGGCCAAAGTAATCCCGCTG
GCCCTGGATCATGTTCGCTG

Product: cobalamin synthesis protein, P47K

Products: NA

Alternate protein names: P47K [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MMPLPLTVVAGYLGAGKTTFINRLLAGDHRLRLMVLVNDFGAINIDAGLIALADGDTIALTNGCVCCTMGADLFMALGDA
LDRRPRPDHLIVEASGVADPARIANAALAEPDLAYGGIVTIVDGQQIDGLCADPMIGAQVRDQIACADLVVVSKVCELSA
VLAATLTTLSGASVLLSADVTDPATLLCAAPKPTPASAPHPDYQSWSYTGDATLHREVLSQSVAKRPQALFRLKGHVMDP
NGGAWEVHAVGRHVSVTQAAPQSHTQLVAIGLKSLVKKDDVDAWWQSATA

Sequences:

>Translated_290_residues
MMPLPLTVVAGYLGAGKTTFINRLLAGDHRLRLMVLVNDFGAINIDAGLIALADGDTIALTNGCVCCTMGADLFMALGDA
LDRRPRPDHLIVEASGVADPARIANAALAEPDLAYGGIVTIVDGQQIDGLCADPMIGAQVRDQIACADLVVVSKVCELSA
VLAATLTTLSGASVLLSADVTDPATLLCAAPKPTPASAPHPDYQSWSYTGDATLHREVLSQSVAKRPQALFRLKGHVMDP
NGGAWEVHAVGRHVSVTQAAPQSHTQLVAIGLKSLVKKDDVDAWWQSATA
>Mature_290_residues
MMPLPLTVVAGYLGAGKTTFINRLLAGDHRLRLMVLVNDFGAINIDAGLIALADGDTIALTNGCVCCTMGADLFMALGDA
LDRRPRPDHLIVEASGVADPARIANAALAEPDLAYGGIVTIVDGQQIDGLCADPMIGAQVRDQIACADLVVVSKVCELSA
VLAATLTTLSGASVLLSADVTDPATLLCAAPKPTPASAPHPDYQSWSYTGDATLHREVLSQSVAKRPQALFRLKGHVMDP
NGGAWEVHAVGRHVSVTQAAPQSHTQLVAIGLKSLVKKDDVDAWWQSATA

Specific function: The presence of P47K is critical for the expression of the nitrile hydratase genes. May stabilize or activate the nitrile hydratase proteins [H]

COG id: COG0523

COG function: function code R; Putative GTPases (G3E family)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 cobW C-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI223941779, Length=217, Percent_Identity=27.6497695852535, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI33469141, Length=193, Percent_Identity=29.0155440414508, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI126722884, Length=165, Percent_Identity=30.3030303030303, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI146231952, Length=193, Percent_Identity=28.4974093264249, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI148727351, Length=165, Percent_Identity=29.6969696969697, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI119120938, Length=132, Percent_Identity=30.3030303030303, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI223941776, Length=154, Percent_Identity=29.2207792207792, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1788499, Length=152, Percent_Identity=33.5526315789474, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082430, Length=162, Percent_Identity=31.4814814814815, Blast_Score=74, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6324356, Length=134, Percent_Identity=29.1044776119403, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003495
- InterPro:   IPR011629 [H]

Pfam domain/function: PF02492 cobW; PF07683 CobW_C [H]

EC number: NA

Molecular weight: Translated: 30258; Mature: 30258

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPLPLTVVAGYLGAGKTTFINRLLAGDHRLRLMVLVNDFGAINIDAGLIALADGDTIAL
CCCCCHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEEEECCCEEEECCCEEEEECCCEEEE
TNGCVCCTMGADLFMALGDALDRRPRPDHLIVEASGVADPARIANAALAEPDLAYGGIVT
ECCEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEE
IVDGQQIDGLCADPMIGAQVRDQIACADLVVVSKVCELSAVLAATLTTLSGASVLLSADV
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
TDPATLLCAAPKPTPASAPHPDYQSWSYTGDATLHREVLSQSVAKRPQALFRLKGHVMDP
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHCEEECC
NGGAWEVHAVGRHVSVTQAAPQSHTQLVAIGLKSLVKKDDVDAWWQSATA
CCCEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCC
>Mature Secondary Structure
MMPLPLTVVAGYLGAGKTTFINRLLAGDHRLRLMVLVNDFGAINIDAGLIALADGDTIAL
CCCCCHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEEEECCCEEEECCCEEEEECCCEEEE
TNGCVCCTMGADLFMALGDALDRRPRPDHLIVEASGVADPARIANAALAEPDLAYGGIVT
ECCEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEE
IVDGQQIDGLCADPMIGAQVRDQIACADLVVVSKVCELSAVLAATLTTLSGASVLLSADV
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
TDPATLLCAAPKPTPASAPHPDYQSWSYTGDATLHREVLSQSVAKRPQALFRLKGHVMDP
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHCEEECC
NGGAWEVHAVGRHVSVTQAAPQSHTQLVAIGLKSLVKKDDVDAWWQSATA
CCCEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2013568 [H]