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The map label for this gene is rihC [H]

Identifier: 89055723

GI number: 89055723

Start: 3279184

End: 3280140

Strand: Direct

Name: rihC [H]

Synonym: Jann_3232

Alternate gene names: 89055723

Gene position: 3279184-3280140 (Clockwise)

Preceding gene: 89055721

Following gene: 89055724

Centisome position: 75.94

GC content: 62.59

Gene sequence:

>957_bases
ATGAAACGAATGTGCATTCTCGATACCGATGGGGGCGTCGACGATGCACAGGCCTTGCTGATGCTGATTGCGGCAGGCCG
GGCACCGGATCTAATAACCACGGTGTTTGGCAATGTCGGATTGCCTGCTGCCACGCGCAATATCCTGACAGTGCTGGCCG
TGGCCGACCGGGCCGACATCCCTGTCCATGCGGGTGCGGGCGAACCGCTGACGCAGCCGATCATGGATGCCACCCAGATC
CACGGGGCCGACGGCCTCGGCGGCGCACCGCGGCCGTCCGTGATCCCGGACCCCACCGGACAGGACGCCGTGCAGATCCT
CGTGTCGCTATTGCAAAAGGCGGCGATGGACGGCGAAAAGGTCGACTTTCTGATGATCGGTCCGCTGACCAATCTGGCAC
TGGTGTTGCAACAAGCGCCCGACTGCAATGCCGGGATCGGGCGGGTGACGATCATGGGTGGCACACTTCACGGGCGGGGC
AACGTGACCCCGGCGGCCGAGTTCAACATCTTCGCTGATCCGGAGGCTGCCGCCATTGTCTTCGCTGCCGACATCGAAAT
CACCCTTGTCCCGTGGGAGGTTTGCACAGCCCACCGGATCACAGGGGCAGAGGTGGACGCGCTGTTTGAAACATCCGGCG
AGGGCAGACTGAAGAGATTCTCGCATGCATTGGTCGCCCACGCGCGATCTGTAAATATTGGGTTTGGTGGTGACGACCAT
TTCCGGTTCGTGGACCCATTGGCCGCAGCGGTGATGATTGACCCGGGCATTGTCACCAGATCTCTGAACGCCAGCATCGA
TGTTGCCTTGGCCCCGGGTGTCACGCGCGGCATGACGGTGGTCGATCCCTCCGGTCGCCTTGGGACTCCCCCACGAACCT
TGGTGGAGGCCGTCGACATGGGCCGGTTGGTCGAGCTCTACCGGGCATCCATTGCCGGTCCGGCGTCGACAAAATGA

Upstream 100 bases:

>100_bases
TTCAAACCGCCGACGTCCGCCACCCGTGCGACCTATGGACTTATCAGTGGTCGTCCTATCTTTTGCGATCTGACATTCAT
CCTTTTGATCGGCCGCTTCC

Downstream 100 bases:

>100_bases
CCGACCAACCCGCCCCGACAGACCACCTGATCCGAGCCGCCGATGAGGTGAAGATCCGCCAGCGGCTGGTGCGCGTGGCG
CTTGGCCATGTGGCAGGTGA

Product: inosine/uridine-preferring nucleoside hydrolase

Products: D-ribose; purine

Alternate protein names: Purine/pyrimidine ribonucleoside hydrolase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKRMCILDTDGGVDDAQALLMLIAAGRAPDLITTVFGNVGLPAATRNILTVLAVADRADIPVHAGAGEPLTQPIMDATQI
HGADGLGGAPRPSVIPDPTGQDAVQILVSLLQKAAMDGEKVDFLMIGPLTNLALVLQQAPDCNAGIGRVTIMGGTLHGRG
NVTPAAEFNIFADPEAAAIVFAADIEITLVPWEVCTAHRITGAEVDALFETSGEGRLKRFSHALVAHARSVNIGFGGDDH
FRFVDPLAAAVMIDPGIVTRSLNASIDVALAPGVTRGMTVVDPSGRLGTPPRTLVEAVDMGRLVELYRASIAGPASTK

Sequences:

>Translated_318_residues
MKRMCILDTDGGVDDAQALLMLIAAGRAPDLITTVFGNVGLPAATRNILTVLAVADRADIPVHAGAGEPLTQPIMDATQI
HGADGLGGAPRPSVIPDPTGQDAVQILVSLLQKAAMDGEKVDFLMIGPLTNLALVLQQAPDCNAGIGRVTIMGGTLHGRG
NVTPAAEFNIFADPEAAAIVFAADIEITLVPWEVCTAHRITGAEVDALFETSGEGRLKRFSHALVAHARSVNIGFGGDDH
FRFVDPLAAAVMIDPGIVTRSLNASIDVALAPGVTRGMTVVDPSGRLGTPPRTLVEAVDMGRLVELYRASIAGPASTK
>Mature_318_residues
MKRMCILDTDGGVDDAQALLMLIAAGRAPDLITTVFGNVGLPAATRNILTVLAVADRADIPVHAGAGEPLTQPIMDATQI
HGADGLGGAPRPSVIPDPTGQDAVQILVSLLQKAAMDGEKVDFLMIGPLTNLALVLQQAPDCNAGIGRVTIMGGTLHGRG
NVTPAAEFNIFADPEAAAIVFAADIEITLVPWEVCTAHRITGAEVDALFETSGEGRLKRFSHALVAHARSVNIGFGGDDH
FRFVDPLAAAVMIDPGIVTRSLNASIDVALAPGVTRGMTVVDPSGRLGTPPRTLVEAVDMGRLVELYRASIAGPASTK

Specific function: Hydrolyzes both purine and pyrimidine ribonucleosides with a broad-substrate specificity [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihC subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=306, Percent_Identity=34.3137254901961, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1786213, Length=261, Percent_Identity=38.6973180076628, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1788486, Length=300, Percent_Identity=34.3333333333333, Blast_Score=114, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI32565474, Length=286, Percent_Identity=31.4685314685315, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17565698, Length=311, Percent_Identity=30.5466237942122, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6320608, Length=183, Percent_Identity=37.1584699453552, Blast_Score=90, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24583915, Length=305, Percent_Identity=32.7868852459016, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24641839, Length=232, Percent_Identity=37.0689655172414, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24641837, Length=337, Percent_Identity=29.9703264094956, Blast_Score=95, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022976 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: 3.2.2.1

Molecular weight: Translated: 32968; Mature: 32968

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRMCILDTDGGVDDAQALLMLIAAGRAPDLITTVFGNVGLPAATRNILTVLAVADRADI
CCCEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHEEEEEECCCCCC
PVHAGAGEPLTQPIMDATQIHGADGLGGAPRPSVIPDPTGQDAVQILVSLLQKAAMDGEK
CEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
VDFLMIGPLTNLALVLQQAPDCNAGIGRVTIMGGTLHGRGNVTPAAEFNIFADPEAAAIV
EEEEEECCHHHHHHHHHCCCCCCCCCCEEEEEECEEECCCCCCCCCEEEEEECCCCEEEE
FAADIEITLVPWEVCTAHRITGAEVDALFETSGEGRLKRFSHALVAHARSVNIGFGGDDH
EEECEEEEEEEHHHHHHCCCCCCHHHHEEECCCCCHHHHHHHHHHHHHEEEEECCCCCCC
FRFVDPLAAAVMIDPGIVTRSLNASIDVALAPGVTRGMTVVDPSGRLGTPPRTLVEAVDM
CCHHHHHHHHEEECCCCEEEECCCEEEEEECCCCCCCEEEECCCCCCCCCHHHHHHHHHH
GRLVELYRASIAGPASTK
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKRMCILDTDGGVDDAQALLMLIAAGRAPDLITTVFGNVGLPAATRNILTVLAVADRADI
CCCEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHEEEEEECCCCCC
PVHAGAGEPLTQPIMDATQIHGADGLGGAPRPSVIPDPTGQDAVQILVSLLQKAAMDGEK
CEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
VDFLMIGPLTNLALVLQQAPDCNAGIGRVTIMGGTLHGRGNVTPAAEFNIFADPEAAAIV
EEEEEECCHHHHHHHHHCCCCCCCCCCEEEEEECEEECCCCCCCCCEEEEEECCCCEEEE
FAADIEITLVPWEVCTAHRITGAEVDALFETSGEGRLKRFSHALVAHARSVNIGFGGDDH
EEECEEEEEEEHHHHHHCCCCCCHHHHEEECCCCCHHHHHHHHHHHHHEEEEECCCCCCC
FRFVDPLAAAVMIDPGIVTRSLNASIDVALAPGVTRGMTVVDPSGRLGTPPRTLVEAVDM
CCHHHHHHHHEEECCCCEEEECCCEEEEEECCCCCCCEEEECCCCCCCCCHHHHHHHHHH
GRLVELYRASIAGPASTK
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-D-ribosylpurine; H2O

Specific reaction: an N-D-ribosylpurine + H2O = D-ribose + a purine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA