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The map label for this gene is pyrG

Identifier: 89055672

GI number: 89055672

Start: 3221921

End: 3223564

Strand: Direct

Name: pyrG

Synonym: Jann_3181

Alternate gene names: 89055672

Gene position: 3221921-3223564 (Clockwise)

Preceding gene: 89055671

Following gene: 89055673

Centisome position: 74.62

GC content: 60.1

Gene sequence:

>1644_bases
ATGGCGCGTTTTATTTTCATCACCGGTGGTGTCGTCTCATCGCTCGGCAAAGGCCTAGCGTCCGCCGCACTGGGTGCGTT
GTTGCAAGCGCGCGGATTTTCGGTGCGGTTGCGCAAGCTAGACCCTTACCTGAACGTGGATCCCGGCACGATGTCGCCCT
TTGAGCATGGAGAGGTGTTTGTCACCGACGATGGGGCGGAAACCGACCTCGACCTCGGCCACTATGAGCGGTTCACGGGC
GTGCCCGCCAGCAAGACGGATTCCATCTCATCGGGCCGGATCTACACCAATGTGTTGGAGAAGGAGCGGCGCGGAGATTA
TCTGGGCAAGACCATTCAGGTCATTCCCCACGTCACCAATGAGATCAAGGATTTCATCAACATAGGCGAAGACGATGTCG
ACTTCATGCTGTGCGAAATCGGCGGCACGGTCGGTGATATCGAAGGCCTGCCGTTTTTTGAGGCGATCCGGCAGTTCAGC
CAGGACAAAGCGCGCGGGCAGTGTATTTTCATGCACCTCACGCTGTTGCCCTTCATCAAGGCGAGCGGGGAGCTGAAGAC
CAAGCCGACCCAGCACAGTGTCAAGGAACTCCGCTCCATCGGGCTGGCGCCTGACATCCTTGTGTGCCGGTCCGAGGGCC
CGATCCCGGAAAAAGAGCGCGAGAAACTGGCGCTGTTCTGCAACGTGCGCCCTGATTCCGTAATCGCGGCGCAGGACTTG
TCGTCGATCTATACCGCGCCGCTGGCCTACCACCGTGAGGGCCTTGATCAAGCCGTGCTCGATGCGTTTGGCATCACCCC
TGCCCCGAAACCCAACCTCAGCATCTGGGAGGATGTGGCCGATCGGATCAACAACCCGGAGGGCGTCGTAAAGGTCGCGA
TCGTCGGCAAATATACGCAGTTGGAGGACGCCTACAAATCCATCGCCGAAGCGCTGACCCACGGCGGCATGGCGAACCGT
GTGAAGGTGGAAATCGAATGGGTCGACGCCGAGACGTTCGAGCGCGAAGACCCGGCCCCGCATCTGCAAGGCTTCCACGC
GATCCTGGTCCCGGGCGGGTTCGGAGAGCGCGGGACGGAAGGTAAAATCAAGGCGGCCGAATTCGCGCGGACCCGGAAAG
TCCCGTATCTGGGGATCTGCCTTGGTATGCAGATGGCGGTGATTGAGGCGGCGCGCAATCTGGCGCAACTGGACGATGCC
GGATCCGAGGAATTCGATCACGAAGCGGGTAAAAAGCGTTTCACACCCGTTGTTTACCATCTGAAAGAATGGGTGCAGGG
CAATCACAAGGTTGAGCGCAAAGTCGGTGACGACAAGGGCGGAACCATGCGTCTGGGGGCCTATGACGCCGTGTTGACCG
AAGGCTCCCGCGTGGCCGAGGCCTACGGGACGACGGCGATTGAGGAACGGCACCGCCACCGCTACGAGGTCGACACGAAA
TACCGGGACGCGTTGGAGGAGAAGGGCCTGATCTTCTCGGGCATGTCACCCGACGGGGCATTGCCCGAGATCGTGGAGGT
CAAGGATCATCCCTGGTTCATCGGCGTTCAATTCCACCCCGAACTGAAGTCCAAGCCCTTTGAGCCACACCCCCTGTTTC
GCGATTTCGTGCGCGCGGCAAAAGAGAATTCGCGCCTCGTCTGA

Upstream 100 bases:

>100_bases
GTGCGCTGGCCCCGAATCCGGGTACTCTCAAATCCCGTGATAATAGCAATGATGGACCGGCCCCGGACGGCCCCACCATC
CGCACGGGAGGTTTTCAGGC

Downstream 100 bases:

>100_bases
GCGCGGGAATTCGGGCCGTCTTAACGAATCCTCAACCCTTCGCGATGAGTATGGGGTGAATGTGCCACATCTTTGCCGCA
ATTGATCACTCGGTGGCGGC

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MARFIFITGGVVSSLGKGLASAALGALLQARGFSVRLRKLDPYLNVDPGTMSPFEHGEVFVTDDGAETDLDLGHYERFTG
VPASKTDSISSGRIYTNVLEKERRGDYLGKTIQVIPHVTNEIKDFINIGEDDVDFMLCEIGGTVGDIEGLPFFEAIRQFS
QDKARGQCIFMHLTLLPFIKASGELKTKPTQHSVKELRSIGLAPDILVCRSEGPIPEKEREKLALFCNVRPDSVIAAQDL
SSIYTAPLAYHREGLDQAVLDAFGITPAPKPNLSIWEDVADRINNPEGVVKVAIVGKYTQLEDAYKSIAEALTHGGMANR
VKVEIEWVDAETFEREDPAPHLQGFHAILVPGGFGERGTEGKIKAAEFARTRKVPYLGICLGMQMAVIEAARNLAQLDDA
GSEEFDHEAGKKRFTPVVYHLKEWVQGNHKVERKVGDDKGGTMRLGAYDAVLTEGSRVAEAYGTTAIEERHRHRYEVDTK
YRDALEEKGLIFSGMSPDGALPEIVEVKDHPWFIGVQFHPELKSKPFEPHPLFRDFVRAAKENSRLV

Sequences:

>Translated_547_residues
MARFIFITGGVVSSLGKGLASAALGALLQARGFSVRLRKLDPYLNVDPGTMSPFEHGEVFVTDDGAETDLDLGHYERFTG
VPASKTDSISSGRIYTNVLEKERRGDYLGKTIQVIPHVTNEIKDFINIGEDDVDFMLCEIGGTVGDIEGLPFFEAIRQFS
QDKARGQCIFMHLTLLPFIKASGELKTKPTQHSVKELRSIGLAPDILVCRSEGPIPEKEREKLALFCNVRPDSVIAAQDL
SSIYTAPLAYHREGLDQAVLDAFGITPAPKPNLSIWEDVADRINNPEGVVKVAIVGKYTQLEDAYKSIAEALTHGGMANR
VKVEIEWVDAETFEREDPAPHLQGFHAILVPGGFGERGTEGKIKAAEFARTRKVPYLGICLGMQMAVIEAARNLAQLDDA
GSEEFDHEAGKKRFTPVVYHLKEWVQGNHKVERKVGDDKGGTMRLGAYDAVLTEGSRVAEAYGTTAIEERHRHRYEVDTK
YRDALEEKGLIFSGMSPDGALPEIVEVKDHPWFIGVQFHPELKSKPFEPHPLFRDFVRAAKENSRLV
>Mature_546_residues
ARFIFITGGVVSSLGKGLASAALGALLQARGFSVRLRKLDPYLNVDPGTMSPFEHGEVFVTDDGAETDLDLGHYERFTGV
PASKTDSISSGRIYTNVLEKERRGDYLGKTIQVIPHVTNEIKDFINIGEDDVDFMLCEIGGTVGDIEGLPFFEAIRQFSQ
DKARGQCIFMHLTLLPFIKASGELKTKPTQHSVKELRSIGLAPDILVCRSEGPIPEKEREKLALFCNVRPDSVIAAQDLS
SIYTAPLAYHREGLDQAVLDAFGITPAPKPNLSIWEDVADRINNPEGVVKVAIVGKYTQLEDAYKSIAEALTHGGMANRV
KVEIEWVDAETFEREDPAPHLQGFHAILVPGGFGERGTEGKIKAAEFARTRKVPYLGICLGMQMAVIEAARNLAQLDDAG
SEEFDHEAGKKRFTPVVYHLKEWVQGNHKVERKVGDDKGGTMRLGAYDAVLTEGSRVAEAYGTTAIEERHRHRYEVDTKY
RDALEEKGLIFSGMSPDGALPEIVEVKDHPWFIGVQFHPELKSKPFEPHPLFRDFVRAAKENSRLV

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=560, Percent_Identity=43.3928571428571, Blast_Score=474, Evalue=1e-134,
Organism=Homo sapiens, GI28559085, Length=567, Percent_Identity=43.2098765432099, Blast_Score=467, Evalue=1e-131,
Organism=Homo sapiens, GI28559083, Length=567, Percent_Identity=43.2098765432099, Blast_Score=467, Evalue=1e-131,
Organism=Homo sapiens, GI221316689, Length=567, Percent_Identity=43.2098765432099, Blast_Score=467, Evalue=1e-131,
Organism=Escherichia coli, GI1789142, Length=542, Percent_Identity=54.4280442804428, Blast_Score=568, Evalue=1e-163,
Organism=Caenorhabditis elegans, GI25148299, Length=612, Percent_Identity=37.9084967320261, Blast_Score=419, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6322563, Length=569, Percent_Identity=41.4762741652021, Blast_Score=445, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6319432, Length=572, Percent_Identity=41.4335664335664, Blast_Score=436, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24664469, Length=560, Percent_Identity=43.2142857142857, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI21357815, Length=505, Percent_Identity=42.5742574257426, Blast_Score=394, Evalue=1e-110,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_JANSC (Q28MG4)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_511123.1
- HSSP:   P17812
- ProteinModelPortal:   Q28MG4
- SMR:   Q28MG4
- STRING:   Q28MG4
- GeneID:   3935652
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_3181
- eggNOG:   COG0504
- HOGENOM:   HBG597806
- OMA:   IIHAGAN
- PhylomeDB:   Q28MG4
- ProtClustDB:   PRK05380
- BioCyc:   JSP290400:JANN_3181-MONOMER
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 60528; Mature: 60397

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 380-380 ACT_SITE 519-519 ACT_SITE 521-521

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARFIFITGGVVSSLGKGLASAALGALLQARGFSVRLRKLDPYLNVDPGTMSPFEHGEVF
CCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGVPASKTDSISSGRIYTNVLEKERRGDYLGKTIQVIPHVTN
EECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
EIKDFINIGEDDVDFMLCEIGGTVGDIEGLPFFEAIRQFSQDKARGQCIFMHLTLLPFIK
HHHHHHHCCCCCHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHH
ASGELKTKPTQHSVKELRSIGLAPDILVCRSEGPIPEKEREKLALFCNVRPDSVIAAQDL
CCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCHHCCCCEEEEEECCCCCEEHHHHH
SSIYTAPLAYHREGLDQAVLDAFGITPAPKPNLSIWEDVADRINNPEGVVKVAIVGKYTQ
HHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCHHH
LEDAYKSIAEALTHGGMANRVKVEIEWVDAETFEREDPAPHLQGFHAILVPGGFGERGTE
HHHHHHHHHHHHHHCCCCCEEEEEEEEECCHHCCCCCCCHHHCCCEEEEECCCCCCCCCC
GKIKAAEFARTRKVPYLGICLGMQMAVIEAARNLAQLDDAGSEEFDHEAGKKRFTPVVYH
CCEEHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCHHHCCHHHHH
LKEWVQGNHKVERKVGDDKGGTMRLGAYDAVLTEGSRVAEAYGTTAIEERHRHRYEVDTK
HHHHHCCCCHHHHHCCCCCCCEEEECCHHHHHHCCCHHHHHHCCHHHHHHHHHCCCCCHH
YRDALEEKGLIFSGMSPDGALPEIVEVKDHPWFIGVQFHPELKSKPFEPHPLFRDFVRAA
HHHHHHHCCCEEECCCCCCCCCHHEEECCCCEEEEEEECCHHCCCCCCCCHHHHHHHHHH
KENSRLV
HCCCCCC
>Mature Secondary Structure 
ARFIFITGGVVSSLGKGLASAALGALLQARGFSVRLRKLDPYLNVDPGTMSPFEHGEVF
CEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGVPASKTDSISSGRIYTNVLEKERRGDYLGKTIQVIPHVTN
EECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
EIKDFINIGEDDVDFMLCEIGGTVGDIEGLPFFEAIRQFSQDKARGQCIFMHLTLLPFIK
HHHHHHHCCCCCHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHH
ASGELKTKPTQHSVKELRSIGLAPDILVCRSEGPIPEKEREKLALFCNVRPDSVIAAQDL
CCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCHHCCCCEEEEEECCCCCEEHHHHH
SSIYTAPLAYHREGLDQAVLDAFGITPAPKPNLSIWEDVADRINNPEGVVKVAIVGKYTQ
HHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCHHH
LEDAYKSIAEALTHGGMANRVKVEIEWVDAETFEREDPAPHLQGFHAILVPGGFGERGTE
HHHHHHHHHHHHHHCCCCCEEEEEEEEECCHHCCCCCCCHHHCCCEEEEECCCCCCCCCC
GKIKAAEFARTRKVPYLGICLGMQMAVIEAARNLAQLDDAGSEEFDHEAGKKRFTPVVYH
CCEEHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCHHHCCHHHHH
LKEWVQGNHKVERKVGDDKGGTMRLGAYDAVLTEGSRVAEAYGTTAIEERHRHRYEVDTK
HHHHHCCCCHHHHHCCCCCCCEEEECCHHHHHHCCCHHHHHHCCHHHHHHHHHCCCCCHH
YRDALEEKGLIFSGMSPDGALPEIVEVKDHPWFIGVQFHPELKSKPFEPHPLFRDFVRAA
HHHHHHHCCCEEECCCCCCCCCHHEEECCCCEEEEEEECCHHCCCCCCCCHHHHHHHHHH
KENSRLV
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA