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The map label for this gene is purR [H]

Identifier: 89055392

GI number: 89055392

Start: 2920685

End: 2921716

Strand: Direct

Name: purR [H]

Synonym: Jann_2901

Alternate gene names: 89055392

Gene position: 2920685-2921716 (Clockwise)

Preceding gene: 89055388

Following gene: 89055393

Centisome position: 67.64

GC content: 60.37

Gene sequence:

>1032_bases
ATGGCCGACCAACAGATCAGGAATATGGAAGACTTTGCCGCCGTCAGCGGGATTTCACGCCCGACGGTCTCAAAGTACTT
CAACGACCCCCAAAGTGTCCGCGCCACGACACGCGCGCGGATCGAACAGGCCCTCGACCGGTATGATTATCGCCCCAACA
TCTATGCAGTGAACCAGAACCGGCGGTTGACCAAGAACGTCGGCATCATGGTCCCCTATCTTGCGGACCCGTTCTTTGCC
GAAATCGCCCGCAACATCGAGATGATGGTGATCGAGGCTGGGTTTCGCCCGATCCTGCTCAGCTCCCACGGCAGCCCGGA
GCAGGAAGCCGAGAACATGGAGAGTTTGCGGGTCCTGAAACCCGCAGGCGTGTTGATGGCCCCCCTGGGCCGTGCGTCCG
ACCGGAGCGCGGTTGAGAAGTTCTGCCGCGATGTGCCCACGATCCTGTTTGACAGCAACCTGGACGGCATGGGCGAGGCG
TTCATCGGCTCCGACAACGTGAGTTTTGTCGAACAAACAGTGGCTTACATCAGTCAATCTGGCAGCCCGCCATGTTTTTT
CGAGATGAAGAACCCCGCCAACCCCAATGCCAACAAGCGCCGAAATGCCTACATCGCGGCCATGGAGACCCGGGGGCTGG
AGCCGCGTGTCATCAGCATCGATGGCGACGGATGGGCGTTTGAGGAGATTGGACGCATCGGGGCGCATGATGTGTTGCAG
GCCGGGCAATTGCCCACCAACACGGTGATCTGTAGCAACGACCGCCTGGCCATCGGCTTTCTGGCGGCCTGTTACGAGCG
TGGCCTACGGGTGGGCAGCGGACCAGACTGCGCGTTGCGCGTCGCCTCCCACGATGATCACCCGTTTTCGCGCTTCACTT
GCCCGGCGCTCACCACCGCCGCCCATGACTACGATTCGGTGTCCAAAAGCAGTGTCGACACCCTCTTTCAACTGATCGAA
AACGGCGCGCGCCTGCCGCAACGGCATGAAACCCTGTTCCCTGCCAAACTAATCATTCGCGCATCCGCTTGA

Upstream 100 bases:

>100_bases
CGCCCGTTGGGCTCAATCTTTACGCGCGAAAAGATTTTTCTTCTCAAATCGTGGGTCAGCGGATAGCGTCGCGGTGGTGA
AACGGAGGCGTTCTGGTCCT

Downstream 100 bases:

>100_bases
TTTTTCAGCGAAATGAAAACCCTTTCGCCTGAAATTTACGCGCGTAAAGTTTTTCGTTGACCGAACGTGCTGTTAACCGC
AATGTGATTGGACAACATCC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MADQQIRNMEDFAAVSGISRPTVSKYFNDPQSVRATTRARIEQALDRYDYRPNIYAVNQNRRLTKNVGIMVPYLADPFFA
EIARNIEMMVIEAGFRPILLSSHGSPEQEAENMESLRVLKPAGVLMAPLGRASDRSAVEKFCRDVPTILFDSNLDGMGEA
FIGSDNVSFVEQTVAYISQSGSPPCFFEMKNPANPNANKRRNAYIAAMETRGLEPRVISIDGDGWAFEEIGRIGAHDVLQ
AGQLPTNTVICSNDRLAIGFLAACYERGLRVGSGPDCALRVASHDDHPFSRFTCPALTTAAHDYDSVSKSSVDTLFQLIE
NGARLPQRHETLFPAKLIIRASA

Sequences:

>Translated_343_residues
MADQQIRNMEDFAAVSGISRPTVSKYFNDPQSVRATTRARIEQALDRYDYRPNIYAVNQNRRLTKNVGIMVPYLADPFFA
EIARNIEMMVIEAGFRPILLSSHGSPEQEAENMESLRVLKPAGVLMAPLGRASDRSAVEKFCRDVPTILFDSNLDGMGEA
FIGSDNVSFVEQTVAYISQSGSPPCFFEMKNPANPNANKRRNAYIAAMETRGLEPRVISIDGDGWAFEEIGRIGAHDVLQ
AGQLPTNTVICSNDRLAIGFLAACYERGLRVGSGPDCALRVASHDDHPFSRFTCPALTTAAHDYDSVSKSSVDTLFQLIE
NGARLPQRHETLFPAKLIIRASA
>Mature_342_residues
ADQQIRNMEDFAAVSGISRPTVSKYFNDPQSVRATTRARIEQALDRYDYRPNIYAVNQNRRLTKNVGIMVPYLADPFFAE
IARNIEMMVIEAGFRPILLSSHGSPEQEAENMESLRVLKPAGVLMAPLGRASDRSAVEKFCRDVPTILFDSNLDGMGEAF
IGSDNVSFVEQTVAYISQSGSPPCFFEMKNPANPNANKRRNAYIAAMETRGLEPRVISIDGDGWAFEEIGRIGAHDVLQA
GQLPTNTVICSNDRLAIGFLAACYERGLRVGSGPDCALRVASHDDHPFSRFTCPALTTAAHDYDSVSKSSVDTLFQLIEN
GARLPQRHETLFPAKLIIRASA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=341, Percent_Identity=26.6862170087977, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1790369, Length=343, Percent_Identity=25.6559766763848, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1789068, Length=339, Percent_Identity=23.598820058997, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1788474, Length=318, Percent_Identity=21.6981132075472, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786540, Length=340, Percent_Identity=26.1764705882353, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI48994940, Length=300, Percent_Identity=24.6666666666667, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787948, Length=337, Percent_Identity=24.6290801186944, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789202, Length=295, Percent_Identity=23.728813559322, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790715, Length=317, Percent_Identity=22.397476340694, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790689, Length=334, Percent_Identity=23.6526946107784, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37823; Mature: 37692

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADQQIRNMEDFAAVSGISRPTVSKYFNDPQSVRATTRARIEQALDRYDYRPNIYAVNQN
CCCHHHHCHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
RRLTKNVGIMVPYLADPFFAEIARNIEMMVIEAGFRPILLSSHGSPEQEAENMESLRVLK
CEEECCCCEEEHHHCCHHHHHHHCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHCC
PAGVLMAPLGRASDRSAVEKFCRDVPTILFDSNLDGMGEAFIGSDNVSFVEQTVAYISQS
CCCHHCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCEECCCCHHHHHHHHHHHHCC
GSPPCFFEMKNPANPNANKRRNAYIAAMETRGLEPRVISIDGDGWAFEEIGRIGAHDVLQ
CCCCEEEEECCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCCHHHHHCCCHHHHHH
AGQLPTNTVICSNDRLAIGFLAACYERGLRVGSGPDCALRVASHDDHPFSRFTCPALTTA
CCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCEECCHHHHH
AHDYDSVSKSSVDTLFQLIENGARLPQRHETLFPAKLIIRASA
HCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEEEEEECC
>Mature Secondary Structure 
ADQQIRNMEDFAAVSGISRPTVSKYFNDPQSVRATTRARIEQALDRYDYRPNIYAVNQN
CCHHHHCHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
RRLTKNVGIMVPYLADPFFAEIARNIEMMVIEAGFRPILLSSHGSPEQEAENMESLRVLK
CEEECCCCEEEHHHCCHHHHHHHCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHCC
PAGVLMAPLGRASDRSAVEKFCRDVPTILFDSNLDGMGEAFIGSDNVSFVEQTVAYISQS
CCCHHCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCEECCCCHHHHHHHHHHHHCC
GSPPCFFEMKNPANPNANKRRNAYIAAMETRGLEPRVISIDGDGWAFEEIGRIGAHDVLQ
CCCCEEEEECCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCCHHHHHCCCHHHHHH
AGQLPTNTVICSNDRLAIGFLAACYERGLRVGSGPDCALRVASHDDHPFSRFTCPALTTA
CCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCEECCHHHHH
AHDYDSVSKSSVDTLFQLIENGARLPQRHETLFPAKLIIRASA
HCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA