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The map label for this gene is murD

Identifier: 89055254

GI number: 89055254

Start: 2777317

End: 2778699

Strand: Reverse

Name: murD

Synonym: Jann_2763

Alternate gene names: 89055254

Gene position: 2778699-2777317 (Counterclockwise)

Preceding gene: 89055255

Following gene: 89055253

Centisome position: 64.35

GC content: 64.43

Gene sequence:

>1383_bases
ATGGGTTTTGTAGGCCGAAAAGTGGTGGTTCTGGGTCTTGGCCGATCCGGTCTCGCGTCGGCACGCGCATTGCGTGAGGG
CGGGGCCGAGGCGCTGTGCTGGGATGATGGTGAGGCCGGACGTGCCCGTGCGGCGGCTGAAGGCTTCACGATCCATGACC
CCATCCGGGACGGATTGGACGGCGTTGCCTGCCTGGTCACATCGCCCGGTATTCCCCACCTCTACTCGACCCCCCATCGT
GCCATTGTGGCGGCCTATGCGGCGGGCGTGCCGGTGGACAACGACATTGGTCTGTTCTTCCGGTCCCTGGGGCAGGGGGA
TTGGGCGTTCCATGACACGCCCCCGCGCGTTGTTGCGGTGACGGGATCCAACGGGAAATCTACGACCTCTGCCCTGATCG
CGCATATTCTGGAGGAGGCGGGCACGCCGGTTCAACTGGCGGGCAACATCGGGCGCGGTGTCTTGGACATCGAGCCGCCG
ATTGATGGAGAGGTCATTGTGATCGAGCTGTCGTCCTACCAGACGGAACTGGCCCGTGCCCTGACGCCCGACATCGCGGT
CTTCACCAATCTCTCGGCCGATCATCTGGAACGCCACGGTGGCATGGGCGGGTATTTCGCGGCCAAACGACGTCTGTTTT
CCGAAGGCGGACCGGAGCGGGCCGTGATTGGCGTCGATGAGATCGAAGGCCAATATCTGGCTGGTCAATTGGTGGAGGGG
CCGGAAGATGGGCGGCTGATCCGCGTCTCCTCCGGTAAGCTGAGCGGTCCGGGTTGGGATGTTAGCACGCGCAAGGGGTG
GCTGAGCGAGTTTCGAAAGGGTCGCCAGGCGGCCTCCATCGATCTGCGCGGTGTGCCGGGATTGCCCGGTGCTCACAACC
ACCAGAACGCGGCCTGCGCCTACGGCGTCTGTCGGGCCCTTGGTCTGGCCCCCCGGGTGATCGAGGCGGCATTCGCCACG
TTTGAAGGGCTGCCCCACCGCTCCCAGCGCGTTGCCGAGATCAACGGCGTATTTTACGTCAACGACAGCAAGGCCACCAA
TGTGGACGCGGCAACCAAGGCCCTGGGCGCGTTCAAACGCATCCGTTGGATCTGTGGCGGGTTGGAAAAGGACGGCGGGT
TGGAAGCGCTGTGGGAGGCCGCAGGCCATGTCGCCAAGGCCTACGTGATTGGGCGCGAGGCGGAGGCCTTTGCGCTGCAT
CTGGGCGATATTCCCAAAGACGTCTGCGGCGACATGGCCACGGCCGTTGCACGCGCGTCGGCTGATGCACAGCCGGGCGA
TACGGTGCTTCTGGCCCCCGCCGCCGCCAGCTTCGACCAATATGACAGCTTTGAGACGCGCGGCGCGGATTTCATCGCAC
AGGTGGAAAAGCTGCAAAGCTAG

Upstream 100 bases:

>100_bases
GGCCGGTGGCGCGGCGTAACCGTGGTCCGGATCGAGGGAGGCGGGGGGGTGAGATGAGTTGTTCTGCCAAGGTGAAGGGA
CGGGTGCCGTGATCCCGGTG

Downstream 100 bases:

>100_bases
GGAATTCGTGCTAGGGTGCCGCTCGGTAATGGAGGGTCTCATGGGCAAGGTCATTGGCGTGGGCGGGGTCTTCATGACCT
GCGCCGATCCCGCCGCCACA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MGFVGRKVVVLGLGRSGLASARALREGGAEALCWDDGEAGRARAAAEGFTIHDPIRDGLDGVACLVTSPGIPHLYSTPHR
AIVAAYAAGVPVDNDIGLFFRSLGQGDWAFHDTPPRVVAVTGSNGKSTTSALIAHILEEAGTPVQLAGNIGRGVLDIEPP
IDGEVIVIELSSYQTELARALTPDIAVFTNLSADHLERHGGMGGYFAAKRRLFSEGGPERAVIGVDEIEGQYLAGQLVEG
PEDGRLIRVSSGKLSGPGWDVSTRKGWLSEFRKGRQAASIDLRGVPGLPGAHNHQNAACAYGVCRALGLAPRVIEAAFAT
FEGLPHRSQRVAEINGVFYVNDSKATNVDAATKALGAFKRIRWICGGLEKDGGLEALWEAAGHVAKAYVIGREAEAFALH
LGDIPKDVCGDMATAVARASADAQPGDTVLLAPAAASFDQYDSFETRGADFIAQVEKLQS

Sequences:

>Translated_460_residues
MGFVGRKVVVLGLGRSGLASARALREGGAEALCWDDGEAGRARAAAEGFTIHDPIRDGLDGVACLVTSPGIPHLYSTPHR
AIVAAYAAGVPVDNDIGLFFRSLGQGDWAFHDTPPRVVAVTGSNGKSTTSALIAHILEEAGTPVQLAGNIGRGVLDIEPP
IDGEVIVIELSSYQTELARALTPDIAVFTNLSADHLERHGGMGGYFAAKRRLFSEGGPERAVIGVDEIEGQYLAGQLVEG
PEDGRLIRVSSGKLSGPGWDVSTRKGWLSEFRKGRQAASIDLRGVPGLPGAHNHQNAACAYGVCRALGLAPRVIEAAFAT
FEGLPHRSQRVAEINGVFYVNDSKATNVDAATKALGAFKRIRWICGGLEKDGGLEALWEAAGHVAKAYVIGREAEAFALH
LGDIPKDVCGDMATAVARASADAQPGDTVLLAPAAASFDQYDSFETRGADFIAQVEKLQS
>Mature_459_residues
GFVGRKVVVLGLGRSGLASARALREGGAEALCWDDGEAGRARAAAEGFTIHDPIRDGLDGVACLVTSPGIPHLYSTPHRA
IVAAYAAGVPVDNDIGLFFRSLGQGDWAFHDTPPRVVAVTGSNGKSTTSALIAHILEEAGTPVQLAGNIGRGVLDIEPPI
DGEVIVIELSSYQTELARALTPDIAVFTNLSADHLERHGGMGGYFAAKRRLFSEGGPERAVIGVDEIEGQYLAGQLVEGP
EDGRLIRVSSGKLSGPGWDVSTRKGWLSEFRKGRQAASIDLRGVPGLPGAHNHQNAACAYGVCRALGLAPRVIEAAFATF
EGLPHRSQRVAEINGVFYVNDSKATNVDAATKALGAFKRIRWICGGLEKDGGLEALWEAAGHVAKAYVIGREAEAFALHL
GDIPKDVCGDMATAVARASADAQPGDTVLLAPAAASFDQYDSFETRGADFIAQVEKLQS

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=462, Percent_Identity=30.7359307359307, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1786274, Length=268, Percent_Identity=28.7313432835821, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_JANSC (Q28NN2)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_510705.1
- ProteinModelPortal:   Q28NN2
- SMR:   Q28NN2
- STRING:   Q28NN2
- GeneID:   3935227
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_2763
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   PRK01390
- BioCyc:   JSP290400:JANN_2763-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 48294; Mature: 48163

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFVGRKVVVLGLGRSGLASARALREGGAEALCWDDGEAGRARAAAEGFTIHDPIRDGLD
CCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHCCCEECCHHHCCCC
GVACLVTSPGIPHLYSTPHRAIVAAYAAGVPVDNDIGLFFRSLGQGDWAFHDTPPRVVAV
CEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEE
TGSNGKSTTSALIAHILEEAGTPVQLAGNIGRGVLDIEPPIDGEVIVIELSSYQTELARA
ECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCEEECCCCCCCCEEEEEECHHHHHHHHH
LTPDIAVFTNLSADHLERHGGMGGYFAAKRRLFSEGGPERAVIGVDEIEGQYLAGQLVEG
HCCCEEEEECCCHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEHHHHCCCEEECHHCCC
PEDGRLIRVSSGKLSGPGWDVSTRKGWLSEFRKGRQAASIDLRGVPGLPGAHNHQNAACA
CCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHH
YGVCRALGLAPRVIEAAFATFEGLPHRSQRVAEINGVFYVNDSKATNVDAATKALGAFKR
HHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHEEECCEEEECCCCCCCHHHHHHHHHHHHH
IRWICGGLEKDGGLEALWEAAGHVAKAYVIGREAEAFALHLGDIPKDVCGDMATAVARAS
HHHHHCCCCCCCCHHHHHHHHHHHEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHC
ADAQPGDTVLLAPAAASFDQYDSFETRGADFIAQVEKLQS
CCCCCCCEEEEECCCCCCHHHCCCHHCCHHHHHHHHHHCC
>Mature Secondary Structure 
GFVGRKVVVLGLGRSGLASARALREGGAEALCWDDGEAGRARAAAEGFTIHDPIRDGLD
CCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHCCCEECCHHHCCCC
GVACLVTSPGIPHLYSTPHRAIVAAYAAGVPVDNDIGLFFRSLGQGDWAFHDTPPRVVAV
CEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEE
TGSNGKSTTSALIAHILEEAGTPVQLAGNIGRGVLDIEPPIDGEVIVIELSSYQTELARA
ECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCEEECCCCCCCCEEEEEECHHHHHHHHH
LTPDIAVFTNLSADHLERHGGMGGYFAAKRRLFSEGGPERAVIGVDEIEGQYLAGQLVEG
HCCCEEEEECCCHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEHHHHCCCEEECHHCCC
PEDGRLIRVSSGKLSGPGWDVSTRKGWLSEFRKGRQAASIDLRGVPGLPGAHNHQNAACA
CCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHH
YGVCRALGLAPRVIEAAFATFEGLPHRSQRVAEINGVFYVNDSKATNVDAATKALGAFKR
HHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHEEECCEEEECCCCCCCHHHHHHHHHHHHH
IRWICGGLEKDGGLEALWEAAGHVAKAYVIGREAEAFALHLGDIPKDVCGDMATAVARAS
HHHHHCCCCCCCCHHHHHHHHHHHEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHC
ADAQPGDTVLLAPAAASFDQYDSFETRGADFIAQVEKLQS
CCCCCCCEEEEECCCCCCHHHCCCHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA