Click here to switch to the map view.

The map label for this gene is rutR [H]

Identifier: 89055201

GI number: 89055201

Start: 2719777

End: 2720388

Strand: Direct

Name: rutR [H]

Synonym: Jann_2710

Alternate gene names: 89055201

Gene position: 2719777-2720388 (Clockwise)

Preceding gene: 89055188

Following gene: 89055205

Centisome position: 62.99

GC content: 63.07

Gene sequence:

>612_bases
ATGACGGCACCCAAACCCCGCACCCGCATCCAAGAGAAAAACCGTGCCCGTGTGCTGGAAGCGGCGTTGGAGATCTTCTC
GCTCCACGGATTTCGCGGCGCGACGCTGGACGCCATCGCCGCGGAGGCCGGGCTGTCAAAACCCAACCTGCTCTACTATT
TCGCGTCCAAGGAAGAGATCCACGTCACCCTGCTCGGCGGGCTGGTGGATACCTGGCTCGCCCCCTTGCGCGACATGCGC
GCCGATGGACATCCGCAGGACGAGTTGTTGGGATATGTGCGCCGCAAGTTGCAGATGAGCCGGGATTTCCCACGCGAATC
GCGGCTTTTCGCGCAAGAGATCGTTCAGGGCGCCCCCCGCATGATCGACGGATTGTCGACCGATCTGAAGGCGTTGGTGG
ACGACAAGGCCGAGGTGATCCGCGACTGGATGGATGCGGGTCACATTGCACGCTCGGACCCGCACCACCTGATTTTCTCG
ATCTGGTCGCTGACCCAGCATTACGCCGATTTCGACGTTCAGGTCCGCGCGGTCCTTGGCGCGGATCGTGACCCCTTCGC
GGAGGCGGAGGCGTTTCTGGACGTGCTGTTCACCCGCTTGCTGCGCCCCTAG

Upstream 100 bases:

>100_bases
CCCACGCAGTCTCGCGATTGGACGTCGCCAAACGGGCTGCGACGGGCACGGGACAGCCCAATTTGCCTCGCCTTTGGGCA
GTGTTTGAAAAGGCGGACCA

Downstream 100 bases:

>100_bases
GCTTCGATCTTGGACACCCGGAACGGCTCGATCTCGAAGCCCTGCCCATCGGCCTCCGCAGCGTCGAAATCAACGTGCAG
ATGGTTGCCGTCAGGATCCC

Product: TetR family transcriptional regulator

Products: NA

Alternate protein names: Rut operon repressor [H]

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MTAPKPRTRIQEKNRARVLEAALEIFSLHGFRGATLDAIAAEAGLSKPNLLYYFASKEEIHVTLLGGLVDTWLAPLRDMR
ADGHPQDELLGYVRRKLQMSRDFPRESRLFAQEIVQGAPRMIDGLSTDLKALVDDKAEVIRDWMDAGHIARSDPHHLIFS
IWSLTQHYADFDVQVRAVLGADRDPFAEAEAFLDVLFTRLLRP

Sequences:

>Translated_203_residues
MTAPKPRTRIQEKNRARVLEAALEIFSLHGFRGATLDAIAAEAGLSKPNLLYYFASKEEIHVTLLGGLVDTWLAPLRDMR
ADGHPQDELLGYVRRKLQMSRDFPRESRLFAQEIVQGAPRMIDGLSTDLKALVDDKAEVIRDWMDAGHIARSDPHHLIFS
IWSLTQHYADFDVQVRAVLGADRDPFAEAEAFLDVLFTRLLRP
>Mature_202_residues
TAPKPRTRIQEKNRARVLEAALEIFSLHGFRGATLDAIAAEAGLSKPNLLYYFASKEEIHVTLLGGLVDTWLAPLRDMRA
DGHPQDELLGYVRRKLQMSRDFPRESRLFAQEIVQGAPRMIDGLSTDLKALVDDKAEVIRDWMDAGHIARSDPHHLIFSI
WSLTQHYADFDVQVRAVLGADRDPFAEAEAFLDVLFTRLLRP

Specific function: Master transcription regulator which represses the degradation of pyrimidines (rutABCDEFG) and purines (gcl operon) for maintenance of metabolic balance between pyrimidines and purines. It also regulates the synthesis of pyrimidine nucleotides and arginin

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787249, Length=175, Percent_Identity=49.7142857142857, Blast_Score=180, Evalue=6e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR019915
- InterPro:   IPR001647
- InterPro:   IPR013573 [H]

Pfam domain/function: PF08362 TetR_C_3; PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 22990; Mature: 22858

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPKPRTRIQEKNRARVLEAALEIFSLHGFRGATLDAIAAEAGLSKPNLLYYFASKEEI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCE
HVTLLGGLVDTWLAPLRDMRADGHPQDELLGYVRRKLQMSRDFPRESRLFAQEIVQGAPR
EEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHH
MIDGLSTDLKALVDDKAEVIRDWMDAGHIARSDPHHLIFSIWSLTQHYADFDVQVRAVLG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHC
ADRDPFAEAEAFLDVLFTRLLRP
CCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAPKPRTRIQEKNRARVLEAALEIFSLHGFRGATLDAIAAEAGLSKPNLLYYFASKEEI
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCE
HVTLLGGLVDTWLAPLRDMRADGHPQDELLGYVRRKLQMSRDFPRESRLFAQEIVQGAPR
EEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHH
MIDGLSTDLKALVDDKAEVIRDWMDAGHIARSDPHHLIFSIWSLTQHYADFDVQVRAVLG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHC
ADRDPFAEAEAFLDVLFTRLLRP
CCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]