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The map label for this gene is garL [H]

Identifier: 89055182

GI number: 89055182

Start: 2700903

End: 2701667

Strand: Reverse

Name: garL [H]

Synonym: Jann_2691

Alternate gene names: 89055182

Gene position: 2701667-2700903 (Counterclockwise)

Preceding gene: 89055183

Following gene: 89055181

Centisome position: 62.57

GC content: 64.84

Gene sequence:

>765_bases
ATGTCCATCGCGGAATTTCGCACACTTGTCGCCTCCAACCGCCCCCTTGCGGGCACGTTCATGAAGACGCCTTCGGTGGA
TATTCTGGAGGTTCTGATCCTCTCGGGCCTTGATTTCGTGTGCATTGATGCCGAACACGCGCCTTTTGATCGCCAAACCC
TGAACGCCTGCCTCGCGCTGGCGCGGGCCGCTGATTTTCCGGTGCTGATCCGCGTGGCCTCGGGCAAGGCGGAGAATATC
GGCGCAGCGCTTGACCTGGGCGCGGTGGGGATCGTCGTGCCCCATGTCACCGATGCGGACGTCGCCGCGCGGGTCGCCAA
GGCCGCACGCTTCGGCCATGGCGGTCGCGGCTATGCGGGCTCCACCCGGTGGGCCGGATATGCCACGCGCAAGATGCCGG
ATCTTCTGGCGCAAAGTGAGGCCGAGACCGTCGTGATTGCCCAGATCGAGGACCCCGAAGCGGTGGAGGCCTGCGAGACG
ATTGCCGCCACGCCGGGCATCGACGCGCTCTTCCTCGGTCCCGCCGACCTGACCGTCGCCTATGGCCAGACCGATCAGGA
TTGCGATGAGCTTCACGCCGCCATGGCCCGTGTGGGCGAGGCGACATCGACCCATGGCAAGGCCTATATGACGTTCGTGG
CAGATGCAGACCGCGCCAAAGCCCTGCGCAAATATGGCTTCGGGGCCGTCTTTATCGCATCTGAGCATGCCTGGATCCTG
GGTGGCGCCCGGGCGCAAATCGCGGGCATCCGCGAGATAGAGTGA

Upstream 100 bases:

>100_bases
TGCACTCCCCTCCAGATGTTGCAATTCCCCCTTCGGCTCGCCAAACATCGCGCACCCGGGGGCCGTGATCTCCCGGGGCA
GCCTGATCCCGGAGCCCCCC

Downstream 100 bases:

>100_bases
CCGCGCGCCGTTCGCTAAGTTGACCTGCAACCATCGGCATTCCAAGAAATCTGCCTAAAATTTGCCACAATCCTGGCAGG
CTTTCTCCTAAACATCACCT

Product: HpcH/HpaI aldolase

Products: NA

Alternate protein names: KDGluc aldolase; KDGlucA; 2-dehydro-3-deoxy-D-glucarate aldolase; 2-keto-3-deoxy-D-glucarate aldolase; 5-dehydro-4-deoxy-D-glucarate aldolase; Alpha-keto-beta-deoxy-D-glucarate aldolase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSIAEFRTLVASNRPLAGTFMKTPSVDILEVLILSGLDFVCIDAEHAPFDRQTLNACLALARAADFPVLIRVASGKAENI
GAALDLGAVGIVVPHVTDADVAARVAKAARFGHGGRGYAGSTRWAGYATRKMPDLLAQSEAETVVIAQIEDPEAVEACET
IAATPGIDALFLGPADLTVAYGQTDQDCDELHAAMARVGEATSTHGKAYMTFVADADRAKALRKYGFGAVFIASEHAWIL
GGARAQIAGIREIE

Sequences:

>Translated_254_residues
MSIAEFRTLVASNRPLAGTFMKTPSVDILEVLILSGLDFVCIDAEHAPFDRQTLNACLALARAADFPVLIRVASGKAENI
GAALDLGAVGIVVPHVTDADVAARVAKAARFGHGGRGYAGSTRWAGYATRKMPDLLAQSEAETVVIAQIEDPEAVEACET
IAATPGIDALFLGPADLTVAYGQTDQDCDELHAAMARVGEATSTHGKAYMTFVADADRAKALRKYGFGAVFIASEHAWIL
GGARAQIAGIREIE
>Mature_253_residues
SIAEFRTLVASNRPLAGTFMKTPSVDILEVLILSGLDFVCIDAEHAPFDRQTLNACLALARAADFPVLIRVASGKAENIG
AALDLGAVGIVVPHVTDADVAARVAKAARFGHGGRGYAGSTRWAGYATRKMPDLLAQSEAETVVIAQIEDPEAVEACETI
AATPGIDALFLGPADLTVAYGQTDQDCDELHAAMARVGEATSTHGKAYMTFVADADRAKALRKYGFGAVFIASEHAWILG
GARAQIAGIREIE

Specific function: Catalyzes the reversible retro-aldol cleavage of both 5- keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=207, Percent_Identity=32.3671497584541, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788578, Length=175, Percent_Identity=32.5714285714286, Blast_Score=81, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR017648
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.2.20 [H]

Molecular weight: Translated: 26684; Mature: 26552

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIAEFRTLVASNRPLAGTFMKTPSVDILEVLILSGLDFVCIDAEHAPFDRQTLNACLAL
CCHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHH
ARAADFPVLIRVASGKAENIGAALDLGAVGIVVPHVTDADVAARVAKAARFGHGGRGYAG
HHHCCCCEEEEEECCCCCCCCCEECCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCC
STRWAGYATRKMPDLLAQSEAETVVIAQIEDPEAVEACETIAATPGIDALFLGPADLTVA
CCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCEEEECCCCEEEE
YGQTDQDCDELHAAMARVGEATSTHGKAYMTFVADADRAKALRKYGFGAVFIASEHAWIL
ECCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHCCCEEEEECCCEEEE
GGARAQIAGIREIE
ECCHHHCCCCCCCC
>Mature Secondary Structure 
SIAEFRTLVASNRPLAGTFMKTPSVDILEVLILSGLDFVCIDAEHAPFDRQTLNACLAL
CHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHH
ARAADFPVLIRVASGKAENIGAALDLGAVGIVVPHVTDADVAARVAKAARFGHGGRGYAG
HHHCCCCEEEEEECCCCCCCCCEECCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCC
STRWAGYATRKMPDLLAQSEAETVVIAQIEDPEAVEACETIAATPGIDALFLGPADLTVA
CCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCEEEECCCCEEEE
YGQTDQDCDELHAAMARVGEATSTHGKAYMTFVADADRAKALRKYGFGAVFIASEHAWIL
ECCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHCCCEEEEECCCEEEE
GGARAQIAGIREIE
ECCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA