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The map label for this gene is dhbF [H]

Identifier: 89055107

GI number: 89055107

Start: 2635047

End: 2637761

Strand: Reverse

Name: dhbF [H]

Synonym: Jann_2616

Alternate gene names: 89055107

Gene position: 2637761-2635047 (Counterclockwise)

Preceding gene: 89055117

Following gene: 89055105

Centisome position: 61.09

GC content: 67.07

Gene sequence:

>2715_bases
ATGTCAGATCGCGATCGCACGGTCACCTATGGCGGGCTTGGCCAGTTCGCGACACAACTGGGCGTGCATCTGGAAACGCC
GCAGGTTGTGGGCGTCTTTGGCGCGCCGGGCGTTGCCATGGCGGCGAGTGCGGTGGCCTGCGTGGTCCATGGCCGCCCCT
TTGTGCACCTTGACCCGGCCATGCCGCAGATGGTGCTGCACAATATCGTCTCGGAATTGCAGGTCAGCCTGATTGTCACC
TGCCAGCGCGCGGATCCGGGGCAGCTGCCCCGCGACTGCGCCACGATCGACGCCAATGACCTGCTGAATGCCGCGCCCGC
GCCGTATGCTCCGCTGCAAGCGGCCCCCGTTGCGCCGGACGATCCCATCTATCTGGTGGCCACGTCCGGCACGACGGGGC
GCCCCAAGTGCATTCCCGTGGCCCAGGACGCGGCCTATCTGTCGTATGCTTGGCGCGACGCGTTCACGCCCTACGGCCCG
GACACGCGCGTCGGTATCTATGTCTTCGCGATCTGGGAGATGTTCCGCCCGCTGCGCAAGGGGGCGGGGCTGTGGTTTCC
CGATGCCAGCACGTTGTTCGCGCCGGGACAGCTGGCCGATTTTCTGATCGCCCACGATATCGACGAGATGCTGTTCACGC
CGTCGTTCTACGACACGTTCCTGAATGCGCTGGACGGCGACAAGGCGGCGGCCCTTCCCCTCAGCCGGGTGATCCTGAAC
GGCGAGGTTGTCGGCGATGACCTGATCATCGCGTCGCTGGCCAAGCTGCCGGGCACGGCGCTGTGGAACCTCTACAGCAT
CTGCGAGACCCATGATGTCTGCATGTCCCATCTGACAGAACCCGCTGGCGATGGGCCCGCGTCGGTGGGTGTCCCGATGG
AGCATCTGCGCGCCGTCATTCTGGATCAGGCCGACGCCCCATGCCCGCCCGGCACCTCGGGCCAGCTGCATTTCGAGGGG
CCAAGGATGCTGGGGCCCGGCTACGTCAACCGGCCCGAGGAAACCCGCCTGCGGTTTCGCGAGGTGACGCTTCAGGGGCG
TGAGACGCGCCTTTACGACACCGGCGATCAGGCGTGGGTGGATGCCACCGGTGCGCTGCATATCGAGGGGCGCATCGCCC
ATATGCTGAAGCTGCGCGGGTTCTCGGTCCAGACGCGGGAGCTGACGGACACGATGCGCGACCGGCTGGCGTTTTCCAGC
GCCGCGCCCTGGGTGGCAGAGGTCGGCGCGCGCGGTCAAAGCCTGATCTTCTACTTCGCCGCTGACGCCGCACAGGCAAG
GCGCAATGCGGAACAATGGGGCCTGAAGGCGGGAACCAACAAGATGCCAGCGGCCCTTGCCGCCGATCTGCGCGCCGTGT
TGCCGGCCTATTGCGTGCCGTCGTTCCTCGTGCAGATGGACGCGCTGCCCCTGCATCCCGTGTCGGGCAAGGCGGATCTG
CGCGCGCTGCCTGCGGTTGAGGAGGACGACCACGGATCAGACTTCGCTGAGGATGCCGTGATTGCCGCCGCCGCCCTCGC
CATGTCCTGTGCGCCGTCGCAGATCGACCCGGCGCTGAGTTTCCACGATCAGGGTGGCGATTCACTGATGTGCGTGACCC
TGATGCTGGAGCTGGAGAAGACCTACGGCCTGCCCATCGACTTCGATCTGGCGATGAATGTGCCGCTGCACCGGCTCGAC
CAGTTGATGACGCAGGAGGCCGACGCCCCCGCCCCGGCGGAAGACTTCGGCCGCCCGGGCATCCTTTTGACCGGCGCGAC
GGGGTTTCTGGGTGGCCATGTTCTGGCAAAAGCGGCACGCGATCTGCCGGAGGGAGAGGTCGTTTATTGCCTTGTCAGAG
ACAAGAACCGGGGGGCCAGGGACCGGCTGGATGAGGCGGCGCAGTCCCATGGGGTCGCCCCTGATCGCTACGTGATCGTC
CATGGCACCCTGGATGCGCCCCGCTTCGCTTTGGAGGCCGATGCCTATGCCGCGTTGAGCGGTTCGGTCAGCCGCGTCAT
CCACTGCGCGGCGATGGTGAACCTCGCCATCGGGCGGCAGGAGATGCTGGATTGGTCGGCGCGCGGGATGGACACCGTCC
TGACCTTCTGCGCCGATGCCCGGGCCGACCTGCGCTTCACCTCCTCAAGCGCGGTCTTCCCGGACCGGGGCGGGCCGTGG
CCCGAAGCTCCCGCGACCCCGTGGGACGGCTGCACCGGCTATGGCGCGGCCAAGATCGCGGCGGAGGCGGCAATTCGCGC
CGCCGACATCCCGGCGGCCATCGTGCGCCTGCCGTCGCTCTATGATCTGGACGCGCCCAACCCGCGCGACATCTATGAGA
TCATCCTCAAGGCGTCCCTGCGCGCGGGCCACATGCCGCAGGCGCTTGAATTTCCGATGACCGATGTGACCGCCGCCGCC
GCCTTCCTGCTCGGCCCGATCACGGCCCCGGACGCCCCGATCTACAACCTGATGGCCGATGCGCGCATCGCGCCGGACGA
CCCAAACGCGCTGCCCGCGACGGACTGGCTGGCCAGGGTCACACTGGACCCCGGCATCGCCAAGGTCATCGCCGACTTCC
CCGACACCCTTTGCGCCTCTTCCGACTTTGAGAACACAGCCGCCCGTGCTGCTTGGGAACGGGTCAGCGCCCTTCCATTC
AACGAAATCAGTGACAGCGAAGCCCTGCTAACGCGCCGCGCCCGCGACTACCAAAGCGATCCCGCGTTGACCTGA

Upstream 100 bases:

>100_bases
ACGTCTGCGGGAACACACAGGGGCTGATTTCATGATTTCAACTCAGACCGTCGCCGCCGAGCTTGTTCGCGCCATCACGG
ATTTTGCGGATCTGGCCGCC

Downstream 100 bases:

>100_bases
TAATCTTCCATGGTCAGCGCGCGGGCGGCGTCGCTGGCCAGAAAGGCGGCAAGCTCGGCCACTTCGGTGGGCTGAACAAG
CTGGTTTTGCGGGTTCGACT

Product: AMP-dependent synthetase and ligase

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: NA

Number of amino acids: Translated: 904; Mature: 903

Protein sequence:

>904_residues
MSDRDRTVTYGGLGQFATQLGVHLETPQVVGVFGAPGVAMAASAVACVVHGRPFVHLDPAMPQMVLHNIVSELQVSLIVT
CQRADPGQLPRDCATIDANDLLNAAPAPYAPLQAAPVAPDDPIYLVATSGTTGRPKCIPVAQDAAYLSYAWRDAFTPYGP
DTRVGIYVFAIWEMFRPLRKGAGLWFPDASTLFAPGQLADFLIAHDIDEMLFTPSFYDTFLNALDGDKAAALPLSRVILN
GEVVGDDLIIASLAKLPGTALWNLYSICETHDVCMSHLTEPAGDGPASVGVPMEHLRAVILDQADAPCPPGTSGQLHFEG
PRMLGPGYVNRPEETRLRFREVTLQGRETRLYDTGDQAWVDATGALHIEGRIAHMLKLRGFSVQTRELTDTMRDRLAFSS
AAPWVAEVGARGQSLIFYFAADAAQARRNAEQWGLKAGTNKMPAALAADLRAVLPAYCVPSFLVQMDALPLHPVSGKADL
RALPAVEEDDHGSDFAEDAVIAAAALAMSCAPSQIDPALSFHDQGGDSLMCVTLMLELEKTYGLPIDFDLAMNVPLHRLD
QLMTQEADAPAPAEDFGRPGILLTGATGFLGGHVLAKAARDLPEGEVVYCLVRDKNRGARDRLDEAAQSHGVAPDRYVIV
HGTLDAPRFALEADAYAALSGSVSRVIHCAAMVNLAIGRQEMLDWSARGMDTVLTFCADARADLRFTSSSAVFPDRGGPW
PEAPATPWDGCTGYGAAKIAAEAAIRAADIPAAIVRLPSLYDLDAPNPRDIYEIILKASLRAGHMPQALEFPMTDVTAAA
AFLLGPITAPDAPIYNLMADARIAPDDPNALPATDWLARVTLDPGIAKVIADFPDTLCASSDFENTAARAAWERVSALPF
NEISDSEALLTRRARDYQSDPALT

Sequences:

>Translated_904_residues
MSDRDRTVTYGGLGQFATQLGVHLETPQVVGVFGAPGVAMAASAVACVVHGRPFVHLDPAMPQMVLHNIVSELQVSLIVT
CQRADPGQLPRDCATIDANDLLNAAPAPYAPLQAAPVAPDDPIYLVATSGTTGRPKCIPVAQDAAYLSYAWRDAFTPYGP
DTRVGIYVFAIWEMFRPLRKGAGLWFPDASTLFAPGQLADFLIAHDIDEMLFTPSFYDTFLNALDGDKAAALPLSRVILN
GEVVGDDLIIASLAKLPGTALWNLYSICETHDVCMSHLTEPAGDGPASVGVPMEHLRAVILDQADAPCPPGTSGQLHFEG
PRMLGPGYVNRPEETRLRFREVTLQGRETRLYDTGDQAWVDATGALHIEGRIAHMLKLRGFSVQTRELTDTMRDRLAFSS
AAPWVAEVGARGQSLIFYFAADAAQARRNAEQWGLKAGTNKMPAALAADLRAVLPAYCVPSFLVQMDALPLHPVSGKADL
RALPAVEEDDHGSDFAEDAVIAAAALAMSCAPSQIDPALSFHDQGGDSLMCVTLMLELEKTYGLPIDFDLAMNVPLHRLD
QLMTQEADAPAPAEDFGRPGILLTGATGFLGGHVLAKAARDLPEGEVVYCLVRDKNRGARDRLDEAAQSHGVAPDRYVIV
HGTLDAPRFALEADAYAALSGSVSRVIHCAAMVNLAIGRQEMLDWSARGMDTVLTFCADARADLRFTSSSAVFPDRGGPW
PEAPATPWDGCTGYGAAKIAAEAAIRAADIPAAIVRLPSLYDLDAPNPRDIYEIILKASLRAGHMPQALEFPMTDVTAAA
AFLLGPITAPDAPIYNLMADARIAPDDPNALPATDWLARVTLDPGIAKVIADFPDTLCASSDFENTAARAAWERVSALPF
NEISDSEALLTRRARDYQSDPALT
>Mature_903_residues
SDRDRTVTYGGLGQFATQLGVHLETPQVVGVFGAPGVAMAASAVACVVHGRPFVHLDPAMPQMVLHNIVSELQVSLIVTC
QRADPGQLPRDCATIDANDLLNAAPAPYAPLQAAPVAPDDPIYLVATSGTTGRPKCIPVAQDAAYLSYAWRDAFTPYGPD
TRVGIYVFAIWEMFRPLRKGAGLWFPDASTLFAPGQLADFLIAHDIDEMLFTPSFYDTFLNALDGDKAAALPLSRVILNG
EVVGDDLIIASLAKLPGTALWNLYSICETHDVCMSHLTEPAGDGPASVGVPMEHLRAVILDQADAPCPPGTSGQLHFEGP
RMLGPGYVNRPEETRLRFREVTLQGRETRLYDTGDQAWVDATGALHIEGRIAHMLKLRGFSVQTRELTDTMRDRLAFSSA
APWVAEVGARGQSLIFYFAADAAQARRNAEQWGLKAGTNKMPAALAADLRAVLPAYCVPSFLVQMDALPLHPVSGKADLR
ALPAVEEDDHGSDFAEDAVIAAAALAMSCAPSQIDPALSFHDQGGDSLMCVTLMLELEKTYGLPIDFDLAMNVPLHRLDQ
LMTQEADAPAPAEDFGRPGILLTGATGFLGGHVLAKAARDLPEGEVVYCLVRDKNRGARDRLDEAAQSHGVAPDRYVIVH
GTLDAPRFALEADAYAALSGSVSRVIHCAAMVNLAIGRQEMLDWSARGMDTVLTFCADARADLRFTSSSAVFPDRGGPWP
EAPATPWDGCTGYGAAKIAAEAAIRAADIPAAIVRLPSLYDLDAPNPRDIYEIILKASLRAGHMPQALEFPMTDVTAAAA
FLLGPITAPDAPIYNLMADARIAPDDPNALPATDWLARVTLDPGIAKVIADFPDTLCASSDFENTAARAAWERVSALPFN
EISDSEALLTRRARDYQSDPALT

Specific function: Specifically adenylates threonine and glycine, and loads them onto their corresponding peptidyl carrier domains [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain [H]

Homologues:

Organism=Escherichia coli, GI1786801, Length=515, Percent_Identity=28.3495145631068, Blast_Score=111, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR001031 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding; PF00975 Thioesterase [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 96800; Mature: 96669

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS50075 ACP_DOMAIN ; PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRDRTVTYGGLGQFATQLGVHLETPQVVGVFGAPGVAMAASAVACVVHGRPFVHLDPA
CCCCCCEEEECCHHHHHHHHCCEECCCCEEEEECCCHHHHHHHHHHHEECCCCEEEECCC
MPQMVLHNIVSELQVSLIVTCQRADPGQLPRDCATIDANDLLNAAPAPYAPLQAAPVAPD
CHHHHHHHHHHHHEEEEEEEEECCCCCCCCCHHCCCCHHHHHCCCCCCCCCCCCCCCCCC
DPIYLVATSGTTGRPKCIPVAQDAAYLSYAWRDAFTPYGPDTRVGIYVFAIWEMFRPLRK
CCEEEEEECCCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
GAGLWFPDASTLFAPGQLADFLIAHDIDEMLFTPSFYDTFLNALDGDKAAALPLSRVILN
CCCEECCCCCCEECCHHHHHHHHHHCHHHHHCCCHHHHHHHHHHCCCCHHHCCHHHHEEC
GEVVGDDLIIASLAKLPGTALWNLYSICETHDVCMSHLTEPAGDGPASVGVPMEHLRAVI
CEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
LDQADAPCPPGTSGQLHFEGPRMLGPGYVNRPEETRLRFREVTLQGRETRLYDTGDQAWV
HCCCCCCCCCCCCCEEEECCCCEECCCCCCCCHHHHHHHHHEEECCCCEEEEECCCCCEE
DATGALHIEGRIAHMLKLRGFSVQTRELTDTMRDRLAFSSAAPWVAEVGARGQSLIFYFA
ECCCEEEECHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEEE
ADAAQARRNAEQWGLKAGTNKMPAALAADLRAVLPAYCVPSFLVQMDALPLHPVSGKADL
CCHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
RALPAVEEDDHGSDFAEDAVIAAAALAMSCAPSQIDPALSFHDQGGDSLMCVTLMLELEK
EECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCEEHHHHHHHHHH
TYGLPIDFDLAMNVPLHRLDQLMTQEADAPAPAEDFGRPGILLTGATGFLGGHVLAKAAR
HCCCCEEEHHCCCCCHHHHHHHHHHCCCCCCCHHHCCCCCEEEECCCHHHHHHHHHHHHH
DLPEGEVVYCLVRDKNRGARDRLDEAAQSHGVAPDRYVIVHGTLDAPRFALEADAYAALS
CCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCHHHHHC
GSVSRVIHCAAMVNLAIGRQEMLDWSARGMDTVLTFCADARADLRFTSSSAVFPDRGGPW
CCHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCCC
PEAPATPWDGCTGYGAAKIAAEAAIRAADIPAAIVRLPSLYDLDAPNPRDIYEIILKASL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
RAGHMPQALEFPMTDVTAAAAFLLGPITAPDAPIYNLMADARIAPDDPNALPATDWLARV
CCCCCCCHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHEEE
TLDPGIAKVIADFPDTLCASSDFENTAARAAWERVSALPFNEISDSEALLTRRARDYQSD
ECCCHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCC
PALT
CCCC
>Mature Secondary Structure 
SDRDRTVTYGGLGQFATQLGVHLETPQVVGVFGAPGVAMAASAVACVVHGRPFVHLDPA
CCCCCEEEECCHHHHHHHHCCEECCCCEEEEECCCHHHHHHHHHHHEECCCCEEEECCC
MPQMVLHNIVSELQVSLIVTCQRADPGQLPRDCATIDANDLLNAAPAPYAPLQAAPVAPD
CHHHHHHHHHHHHEEEEEEEEECCCCCCCCCHHCCCCHHHHHCCCCCCCCCCCCCCCCCC
DPIYLVATSGTTGRPKCIPVAQDAAYLSYAWRDAFTPYGPDTRVGIYVFAIWEMFRPLRK
CCEEEEEECCCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
GAGLWFPDASTLFAPGQLADFLIAHDIDEMLFTPSFYDTFLNALDGDKAAALPLSRVILN
CCCEECCCCCCEECCHHHHHHHHHHCHHHHHCCCHHHHHHHHHHCCCCHHHCCHHHHEEC
GEVVGDDLIIASLAKLPGTALWNLYSICETHDVCMSHLTEPAGDGPASVGVPMEHLRAVI
CEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
LDQADAPCPPGTSGQLHFEGPRMLGPGYVNRPEETRLRFREVTLQGRETRLYDTGDQAWV
HCCCCCCCCCCCCCEEEECCCCEECCCCCCCCHHHHHHHHHEEECCCCEEEEECCCCCEE
DATGALHIEGRIAHMLKLRGFSVQTRELTDTMRDRLAFSSAAPWVAEVGARGQSLIFYFA
ECCCEEEECHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEEE
ADAAQARRNAEQWGLKAGTNKMPAALAADLRAVLPAYCVPSFLVQMDALPLHPVSGKADL
CCHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
RALPAVEEDDHGSDFAEDAVIAAAALAMSCAPSQIDPALSFHDQGGDSLMCVTLMLELEK
EECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCEEHHHHHHHHHH
TYGLPIDFDLAMNVPLHRLDQLMTQEADAPAPAEDFGRPGILLTGATGFLGGHVLAKAAR
HCCCCEEEHHCCCCCHHHHHHHHHHCCCCCCCHHHCCCCCEEEECCCHHHHHHHHHHHHH
DLPEGEVVYCLVRDKNRGARDRLDEAAQSHGVAPDRYVIVHGTLDAPRFALEADAYAALS
CCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCHHHHHC
GSVSRVIHCAAMVNLAIGRQEMLDWSARGMDTVLTFCADARADLRFTSSSAVFPDRGGPW
CCHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCCC
PEAPATPWDGCTGYGAAKIAAEAAIRAADIPAAIVRLPSLYDLDAPNPRDIYEIILKASL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
RAGHMPQALEFPMTDVTAAAAFLLGPITAPDAPIYNLMADARIAPDDPNALPATDWLARV
CCCCCCCHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHEEE
TLDPGIAKVIADFPDTLCASSDFENTAARAAWERVSALPFNEISDSEALLTRRARDYQSD
ECCCHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCC
PALT
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11112781; 9384377; 10568751; 8550523 [H]