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The map label for this gene is mtbC [H]

Identifier: 89055041

GI number: 89055041

Start: 2553134

End: 2553841

Strand: Direct

Name: mtbC [H]

Synonym: Jann_2550

Alternate gene names: 89055041

Gene position: 2553134-2553841 (Clockwise)

Preceding gene: 89055039

Following gene: 89055042

Centisome position: 59.13

GC content: 60.03

Gene sequence:

>708_bases
ATGTCTGATCAAGACGACGACATCATCCTGTCCGAGCTGAACGACGAAGATCTTGTCGCGCAGATGTTCGACGACCTTTA
CGACGGCCTCAAGGAAGAGATCGAGGAGGGCGTGAACATCCTGCTTGAGCGGAAGTGGGAGCCCTACCGCGTCCTGACCG
AAGCGCTTGTGGGCGGTATGACCATCGTCGGCAACGACTTCCGCGACGGCATCCTGTTCGTGCCCGAGGTGCTGATGGCG
GCCAACGCGATGAAGGGCGGCATGGCGATCCTGAAGCCACTGCTGATCGAGACGGGCGCGCCCCGCGTGGGCAAGATGGT
CATTGGCACGGTCAAAGGCGACATCCACGACATCGGCAAGAACCTCGTATCGATGATGATGGAAGGCGCGGGCTTTGAGG
TCGTGGATCTGGGCATCAACAATGCCGTGGAAAGCTACCTTGAGGCGCTGGAGACGGAACAGCCTGACATCCTCGGCATG
TCCGCGCTTCTGACGACCACCATGCCCTACATGAAGGTCGTCATCGACACGCTGAATGAGAAGGGCATCCGCGACGATTA
CATCGTTCTGGTGGGCGGTGCGCCCCTGAACGACGAATTCGGCAAGGCCATCGGCGCAGATGCCTATTGTCGCGATGCTG
CCGTGGCGGTCGAAACGGCCAAGGAATGGGTGGCTCGCAAGCATAACCAGATGAGCGCCTCGGCCTAG

Upstream 100 bases:

>100_bases
CCGACGCTCCATGTCGCAAACAGCCGTCAACCGGAAGGCGATTTCTTTAGATACTTGAAAAAACACCGGCAGGCCCGCCG
GTTAGCAGGAGCATCCTTCC

Downstream 100 bases:

>100_bases
ATCGCCCCGTCTGTTATGATAGGCCTGCGAGGGGACGCCCCGTCGCAGGCCTTTCCATGTCCGGATGTCCGATGACCCGT
GTTTTCCTTGCCGAACCCCA

Product: 5-methyltetrahydrofolate--homocysteine S-methyltransferase

Products: L-Methionine; 5,6,7,8-Tetrahydrofolate [C]

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MSDQDDDIILSELNDEDLVAQMFDDLYDGLKEEIEEGVNILLERKWEPYRVLTEALVGGMTIVGNDFRDGILFVPEVLMA
ANAMKGGMAILKPLLIETGAPRVGKMVIGTVKGDIHDIGKNLVSMMMEGAGFEVVDLGINNAVESYLEALETEQPDILGM
SALLTTTMPYMKVVIDTLNEKGIRDDYIVLVGGAPLNDEFGKAIGADAYCRDAAVAVETAKEWVARKHNQMSASA

Sequences:

>Translated_235_residues
MSDQDDDIILSELNDEDLVAQMFDDLYDGLKEEIEEGVNILLERKWEPYRVLTEALVGGMTIVGNDFRDGILFVPEVLMA
ANAMKGGMAILKPLLIETGAPRVGKMVIGTVKGDIHDIGKNLVSMMMEGAGFEVVDLGINNAVESYLEALETEQPDILGM
SALLTTTMPYMKVVIDTLNEKGIRDDYIVLVGGAPLNDEFGKAIGADAYCRDAAVAVETAKEWVARKHNQMSASA
>Mature_234_residues
SDQDDDIILSELNDEDLVAQMFDDLYDGLKEEIEEGVNILLERKWEPYRVLTEALVGGMTIVGNDFRDGILFVPEVLMAA
NAMKGGMAILKPLLIETGAPRVGKMVIGTVKGDIHDIGKNLVSMMMEGAGFEVVDLGINNAVESYLEALETEQPDILGMS
ALLTTTMPYMKVVIDTLNEKGIRDDYIVLVGGAPLNDEFGKAIGADAYCRDAAVAVETAKEWVARKHNQMSASA

Specific function: Acts as a methyl group carrier between mtbB and mtbA [H]

COG id: COG5012

COG function: function code R; Predicted cobalamin binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=168, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=7e-19,
Organism=Escherichia coli, GI1790450, Length=172, Percent_Identity=35.4651162790698, Blast_Score=101, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17535405, Length=161, Percent_Identity=36.6459627329193, Blast_Score=100, Evalue=8e-22,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR003759
- InterPro:   IPR012741 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2 [H]

EC number: 2.1.1.13 [C]

Molecular weight: Translated: 25574; Mature: 25443

Theoretical pI: Translated: 4.01; Mature: 4.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQDDDIILSELNDEDLVAQMFDDLYDGLKEEIEEGVNILLERKWEPYRVLTEALVGGM
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCE
TIVGNDFRDGILFVPEVLMAANAMKGGMAILKPLLIETGAPRVGKMVIGTVKGDIHDIGK
EEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHEEEHHHHHHHHHHH
NLVSMMMEGAGFEVVDLGINNAVESYLEALETEQPDILGMSALLTTTMPYMKVVIDTLNE
HHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
KGIRDDYIVLVGGAPLNDEFGKAIGADAYCRDAAVAVETAKEWVARKHNQMSASA
CCCCCCEEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SDQDDDIILSELNDEDLVAQMFDDLYDGLKEEIEEGVNILLERKWEPYRVLTEALVGGM
CCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCE
TIVGNDFRDGILFVPEVLMAANAMKGGMAILKPLLIETGAPRVGKMVIGTVKGDIHDIGK
EEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHEEEHHHHHHHHHHH
NLVSMMMEGAGFEVVDLGINNAVESYLEALETEQPDILGMSALLTTTMPYMKVVIDTLNE
HHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
KGIRDDYIVLVGGAPLNDEFGKAIGADAYCRDAAVAVETAKEWVARKHNQMSASA
CCCCCCEEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; Cobinamide; S-adenosyl-L-methionine [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 500-780 [C]

Specific activity: 3.8

Km value (mM): 0.035 {S-methyltetrahydrofolate}} 0.0016 {S-adenosyl-L-methionine}} 0.00065 {S-adenosyl-L-methionine}} 0.06 {methyltetrahydrofolate}} 0.03 {methyltetrahydrofolate}} 0.016 {homocysteine}} [C]

Substrates: 5-Methyltetrahydrofolate; L-Homocysteine [C]

Specific reaction: 5-Methyltetrahydrofolate + L-Homocysteine --> L-Methionine + 5,6,7,8-Tetrahydrofolate [C]

General reaction: Methyl group transfer [C]

Inhibitor: 5, 6-Dimethyl benzimidazole; alpha-Ribazole 3'-phosphate; Be2+; Cobinamide; Propyl iodide; Sr2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10762254; 10852929 [H]