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The map label for this gene is rne [H]

Identifier: 89055030

GI number: 89055030

Start: 2540632

End: 2543304

Strand: Reverse

Name: rne [H]

Synonym: Jann_2539

Alternate gene names: 89055030

Gene position: 2543304-2540632 (Counterclockwise)

Preceding gene: 89055031

Following gene: 89055028

Centisome position: 58.9

GC content: 63.79

Gene sequence:

>2673_bases
ATGGCAAAAACAATGCTCATCGATGCCACCCACGCGGAAGAAACCCGCGTTGTGGTGGCAGATGGAACCAAGGTCGATGA
ATTTGACTTTGAATCGCTAAATAAGCGGCAACTCGCAGGAAACATATATCTGGCCAAGGTGACGCGGGTTGAGCCCTCGT
TGCAGGCGGCCTTTGTGGATTACGGTGGCAATCGCCACGGGTTCCTCGCGTTCTCGGAAATTCACCCCGATTATTATCAG
ATCCCCGTGGCCGACCGTGAAGCGCTTCTGGCCGAAGAACGCGCCTATGCGGAACAGCAGGCCCGGGAGGAGGAGGAGGA
ATCCTCCAAACCCAAGCGCCGTCGCAAGCGGCGTCGTGGTGGTGACACCAAGGCCGAGGAGACTGGCAATGACGCCGTGG
CCAAAGGCACACCCAACGGCATGGATGTGGTCGATCTGGAAGCGGGGGAAGACGCGGCCGCATCCGATGACGCGGATGTC
GAAGGCCTGTCGCCGATGGAGCTGGTGAAAGAAACGCCGGTGGAAGAGCCCGAGGGCGACGCCGACACGACGGATGACGC
GGACGCCGATACGGATGCTGACACTGATACAGATGCGGATACGGACGACAGCGGCGACGACAATTCCGAGGTTGTGGCCG
ATGACGACGACGTGGAGGAAATCCGCCCCGTCCGCAAACCACGCCCGAAGAAGTACAAGATCCAGGAGGTCGTTAAAGTC
CGCCAGATCATGCTGGTGCAGGTCGTCAAGGAAGAGCGGGGCAACAAGGGGGCCGCGCTGACGACATATCTGTCCCTGGC
CGGGCGCTATTGCGTGTTGATGCCCAACACCGCGCGGGGCGGCGGGATTTCCCGCAAGATCACCAACGCCGCCGACCGCA
AGAAGCTGAAAGACATCGCCACGGCGCTGGATGTGCCCCAGGGCGCGGGCCTGATCGTGCGCACGGCAGGCAGCCAGCGC
ACCAAAGTCGAGATCAAGCGCGATTATGAATATCTGCAACGCCAATGGGAACAGATCCGCGAGCTGACGCTGAAATCCAT
CGCGCCCGCACCGATCTATCAGGAAGGCGACCTGATCCACCGCACCATCCGCGACCTTTATAACCGCGATATCGGCGAGG
TTATCGTGGAGGGCGAGGCCGGGTACCGCCACGCCAAGGACTTCATGAAGATGATCATGCCGTCCCACGCCAAGAAGGTG
AAACGGCACGGCGGCGGGGTTCCGCTTTTCGCGAAATACAAGGTGGAATCGTATCTGTCGGATATGTTCAACCCGACCGT
GCAGCTGAAATCCGGCGGCTATATCGTCATCAACATCACCGAGGCGCTGGTGGCTATCGACGTGAACTCGGGCCGGTCCA
CCAAGGAAGGCAGCATCGAGGATACGGCGACGAAAACCAACCTGGAGGCCGCGGAGGAAGTGGCGCGTCAAGTGCGGTTG
CGTGACCTTGCCGGTCTGATCGTCATCGACTTCATCGACATGGATGAACGCCGCAACAACAACGCCGTCGAGAAGCGCAT
CAAGGACAAGCTGAAGACCGACCGGGCGCGCATTCAGGTGGGCCGGATTTCGACCTTCGGCCTGATGGAAATGTCCCGCC
AGCGCCTGCGCCCGGGTATGTTGGAGGCCACGACGCAGGCCTGCCACCATTGCCACGGCACGGGCCTCGTGCGCTCGGAT
GACAGTCTTGCGCTGTCGATCCTGCGCCAGTTGGAAGAGGAAGGGACACGGGGCCGCGCCCGCGAGGTTCTGCTGACTGC
GCCCGTGGGCATCGTCAATTTCCTGATCAACTTCAAACGCGACTATCTGGACGCGATTGAGGCGCGCTCGGGCATGGCGA
TCCGGATCGAGGCCGACGCGAGCCTTGTCGTGCCGGACTACAAGATCGAGAAGTTCAAGACCGCCACCCGCAAGATCACC
GCCGTGGCCCCCGTCGTTTCCATGGACGCGGGCCTGATGGATGACATCGACGAAGATGCCGGTGATGACGTCGTCGAAGA
CGCGGATCAGGTCGATGAGGCGGCTGAAGCGTCTGAAGACGAGCAGCCGAGGAAAAAGCGCCGCCGCCGTCGTGGTGGCC
GGGGCCGCCGTCGGACCTCCGATGAGAATGGAGAGACCGGTGACAGCGCCGAGGACGGATCAGCCGCTGCGGCTGTGCCG
GAGGAGGCGGCTGGAGCCGACCCGTCAACCGATACACCGCCCGAGGCCAGCCCGGAGGGCGAAGAGGAGCAGCCCAAAAA
GCGCCGCCGCTCGCGCCGGGGTGGCCGGGGCCGCAAGTCGTCTGAGGCGCAAGCCGAGACGACGGATGAGGGCAATGGTG
ACGCCATGGAGGCTCAGGATGCGCCAGCGGCAGAGCCAGCAGAGCCTGCGTCGGTGAGCACGCCGGAGCCCGAGGTCGCG
GCTGACGCTGAGCCTGAGATCACGCCGGAGCCGACCCCGGACCCTGAGCCCGAGCCTCAGCCCGAACGGGAGTTGGTTGC
CGCCGATGCGTCGGTCGCCTCAGAGGAACCTGCCCAGGCGGCCGAGCCAACGCCGGAGCCCGAGGCCGCCCCCGAACCAA
CCCCTGAGCCTGTCGCGGCAGAGGCCACGCCCGAACCTGTCGCGGCCCAGGCGGCAGCGGAACCTGACGCCCCGGCCAAA
CCGAAACGGCGCGGATGGTGGTCGCGGGGCTGA

Upstream 100 bases:

>100_bases
CCGGTTCCATCGCTCTAACAGCACAACGTGATACCTCAACAGCGACATTGCACGGGTTCGCCCCGTGCAGGCGCGCGGGG
CTGTCACGAGCAAGGATATC

Downstream 100 bases:

>100_bases
GTTCACGGTCCATTCAAACAAAAAAGGGCGCCTCACCGGGCGCCCTTTTCGCATCAAATGCGGCGACGGGTTCAGCCGGC
GGTGTCCCGGATGATCGACA

Product: ribonuclease E

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 890; Mature: 889

Protein sequence:

>890_residues
MAKTMLIDATHAEETRVVVADGTKVDEFDFESLNKRQLAGNIYLAKVTRVEPSLQAAFVDYGGNRHGFLAFSEIHPDYYQ
IPVADREALLAEERAYAEQQAREEEEESSKPKRRRKRRRGGDTKAEETGNDAVAKGTPNGMDVVDLEAGEDAAASDDADV
EGLSPMELVKETPVEEPEGDADTTDDADADTDADTDTDADTDDSGDDNSEVVADDDDVEEIRPVRKPRPKKYKIQEVVKV
RQIMLVQVVKEERGNKGAALTTYLSLAGRYCVLMPNTARGGGISRKITNAADRKKLKDIATALDVPQGAGLIVRTAGSQR
TKVEIKRDYEYLQRQWEQIRELTLKSIAPAPIYQEGDLIHRTIRDLYNRDIGEVIVEGEAGYRHAKDFMKMIMPSHAKKV
KRHGGGVPLFAKYKVESYLSDMFNPTVQLKSGGYIVINITEALVAIDVNSGRSTKEGSIEDTATKTNLEAAEEVARQVRL
RDLAGLIVIDFIDMDERRNNNAVEKRIKDKLKTDRARIQVGRISTFGLMEMSRQRLRPGMLEATTQACHHCHGTGLVRSD
DSLALSILRQLEEEGTRGRAREVLLTAPVGIVNFLINFKRDYLDAIEARSGMAIRIEADASLVVPDYKIEKFKTATRKIT
AVAPVVSMDAGLMDDIDEDAGDDVVEDADQVDEAAEASEDEQPRKKRRRRRGGRGRRRTSDENGETGDSAEDGSAAAAVP
EEAAGADPSTDTPPEASPEGEEEQPKKRRRSRRGGRGRKSSEAQAETTDEGNGDAMEAQDAPAAEPAEPASVSTPEPEVA
ADAEPEITPEPTPDPEPEPQPERELVAADASVASEEPAQAAEPTPEPEAAPEPTPEPVAAEATPEPVAAQAAAEPDAPAK
PKRRGWWSRG

Sequences:

>Translated_890_residues
MAKTMLIDATHAEETRVVVADGTKVDEFDFESLNKRQLAGNIYLAKVTRVEPSLQAAFVDYGGNRHGFLAFSEIHPDYYQ
IPVADREALLAEERAYAEQQAREEEEESSKPKRRRKRRRGGDTKAEETGNDAVAKGTPNGMDVVDLEAGEDAAASDDADV
EGLSPMELVKETPVEEPEGDADTTDDADADTDADTDTDADTDDSGDDNSEVVADDDDVEEIRPVRKPRPKKYKIQEVVKV
RQIMLVQVVKEERGNKGAALTTYLSLAGRYCVLMPNTARGGGISRKITNAADRKKLKDIATALDVPQGAGLIVRTAGSQR
TKVEIKRDYEYLQRQWEQIRELTLKSIAPAPIYQEGDLIHRTIRDLYNRDIGEVIVEGEAGYRHAKDFMKMIMPSHAKKV
KRHGGGVPLFAKYKVESYLSDMFNPTVQLKSGGYIVINITEALVAIDVNSGRSTKEGSIEDTATKTNLEAAEEVARQVRL
RDLAGLIVIDFIDMDERRNNNAVEKRIKDKLKTDRARIQVGRISTFGLMEMSRQRLRPGMLEATTQACHHCHGTGLVRSD
DSLALSILRQLEEEGTRGRAREVLLTAPVGIVNFLINFKRDYLDAIEARSGMAIRIEADASLVVPDYKIEKFKTATRKIT
AVAPVVSMDAGLMDDIDEDAGDDVVEDADQVDEAAEASEDEQPRKKRRRRRGGRGRRRTSDENGETGDSAEDGSAAAAVP
EEAAGADPSTDTPPEASPEGEEEQPKKRRRSRRGGRGRKSSEAQAETTDEGNGDAMEAQDAPAAEPAEPASVSTPEPEVA
ADAEPEITPEPTPDPEPEPQPERELVAADASVASEEPAQAAEPTPEPEAAPEPTPEPVAAEATPEPVAAQAAAEPDAPAK
PKRRGWWSRG
>Mature_889_residues
AKTMLIDATHAEETRVVVADGTKVDEFDFESLNKRQLAGNIYLAKVTRVEPSLQAAFVDYGGNRHGFLAFSEIHPDYYQI
PVADREALLAEERAYAEQQAREEEEESSKPKRRRKRRRGGDTKAEETGNDAVAKGTPNGMDVVDLEAGEDAAASDDADVE
GLSPMELVKETPVEEPEGDADTTDDADADTDADTDTDADTDDSGDDNSEVVADDDDVEEIRPVRKPRPKKYKIQEVVKVR
QIMLVQVVKEERGNKGAALTTYLSLAGRYCVLMPNTARGGGISRKITNAADRKKLKDIATALDVPQGAGLIVRTAGSQRT
KVEIKRDYEYLQRQWEQIRELTLKSIAPAPIYQEGDLIHRTIRDLYNRDIGEVIVEGEAGYRHAKDFMKMIMPSHAKKVK
RHGGGVPLFAKYKVESYLSDMFNPTVQLKSGGYIVINITEALVAIDVNSGRSTKEGSIEDTATKTNLEAAEEVARQVRLR
DLAGLIVIDFIDMDERRNNNAVEKRIKDKLKTDRARIQVGRISTFGLMEMSRQRLRPGMLEATTQACHHCHGTGLVRSDD
SLALSILRQLEEEGTRGRAREVLLTAPVGIVNFLINFKRDYLDAIEARSGMAIRIEADASLVVPDYKIEKFKTATRKITA
VAPVVSMDAGLMDDIDEDAGDDVVEDADQVDEAAEASEDEQPRKKRRRRRGGRGRRRTSDENGETGDSAEDGSAAAAVPE
EAAGADPSTDTPPEASPEGEEEQPKKRRRSRRGGRGRKSSEAQAETTDEGNGDAMEAQDAPAAEPAEPASVSTPEPEVAA
DAEPEITPEPTPDPEPEPQPERELVAADASVASEEPAQAAEPTPEPEAAPEPTPEPVAAEATPEPVAAQAAAEPDAPAKP
KRRGWWSRG

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. Major endoribonuclease participating in mRNA turnover. It initiates the decay of RNAs by cutting them internally near their 5'-end. It is able to remove poly(A) tails by an endonucleolytic proce

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=402, Percent_Identity=46.5174129353234, Blast_Score=368, Evalue=1e-102,
Organism=Escherichia coli, GI87082235, Length=338, Percent_Identity=34.0236686390533, Blast_Score=184, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 97324; Mature: 97193

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTMLIDATHAEETRVVVADGTKVDEFDFESLNKRQLAGNIYLAKVTRVEPSLQAAFVD
CCCEEEEECCCCCCCEEEEECCCCCCCCCHHHCCHHHHCCCEEEEEEEECCCCHHEEEEC
YGGNRHGFLAFSEIHPDYYQIPVADREALLAEERAYAEQQAREEEEESSKPKRRRKRRRG
CCCCCCCEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
GDTKAEETGNDAVAKGTPNGMDVVDLEAGEDAAASDDADVEGLSPMELVKETPVEEPEGD
CCCCHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCC
ADTTDDADADTDADTDTDADTDDSGDDNSEVVADDDDVEEIRPVRKPRPKKYKIQEVVKV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHCCCCCCCCHHHHHHHHH
RQIMLVQVVKEERGNKGAALTTYLSLAGRYCVLMPNTARGGGISRKITNAADRKKLKDIA
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
TALDVPQGAGLIVRTAGSQRTKVEIKRDYEYLQRQWEQIRELTLKSIAPAPIYQEGDLIH
HHHCCCCCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
RTIRDLYNRDIGEVIVEGEAGYRHAKDFMKMIMPSHAKKVKRHGGGVPLFAKYKVESYLS
HHHHHHHCCCHHHEEECCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEHHHHHHHHH
DMFNPTVQLKSGGYIVINITEALVAIDVNSGRSTKEGSIEDTATKTNLEAAEEVARQVRL
HHCCCEEEEECCCEEEEEEECEEEEEECCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHH
RDLAGLIVIDFIDMDERRNNNAVEKRIKDKLKTDRARIQVGRISTFGLMEMSRQRLRPGM
HHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHCCCCH
LEATTQACHHCHGTGLVRSDDSLALSILRQLEEEGTRGRAREVLLTAPVGIVNFLINFKR
HHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCCCCHHEEEEECCHHHHHHHHHHHH
DYLDAIEARSGMAIRIEADASLVVPDYKIEKFKTATRKITAVAPVVSMDAGLMDDIDEDA
HHHHHHHHCCCCEEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCC
GDDVVEDADQVDEAAEASEDEQPRKKRRRRRGGRGRRRTSDENGETGDSAEDGSAAAAVP
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EEAAGADPSTDTPPEASPEGEEEQPKKRRRSRRGGRGRKSSEAQAETTDEGNGDAMEAQD
HHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APAAEPAEPASVSTPEPEVAADAEPEITPEPTPDPEPEPQPERELVAADASVASEEPAQA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCHHHCCCCCHHH
AEPTPEPEAAPEPTPEPVAAEATPEPVAAQAAAEPDAPAKPKRRGWWSRG
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKTMLIDATHAEETRVVVADGTKVDEFDFESLNKRQLAGNIYLAKVTRVEPSLQAAFVD
CCEEEEECCCCCCCEEEEECCCCCCCCCHHHCCHHHHCCCEEEEEEEECCCCHHEEEEC
YGGNRHGFLAFSEIHPDYYQIPVADREALLAEERAYAEQQAREEEEESSKPKRRRKRRRG
CCCCCCCEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
GDTKAEETGNDAVAKGTPNGMDVVDLEAGEDAAASDDADVEGLSPMELVKETPVEEPEGD
CCCCHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCC
ADTTDDADADTDADTDTDADTDDSGDDNSEVVADDDDVEEIRPVRKPRPKKYKIQEVVKV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHCCCCCCCCHHHHHHHHH
RQIMLVQVVKEERGNKGAALTTYLSLAGRYCVLMPNTARGGGISRKITNAADRKKLKDIA
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
TALDVPQGAGLIVRTAGSQRTKVEIKRDYEYLQRQWEQIRELTLKSIAPAPIYQEGDLIH
HHHCCCCCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
RTIRDLYNRDIGEVIVEGEAGYRHAKDFMKMIMPSHAKKVKRHGGGVPLFAKYKVESYLS
HHHHHHHCCCHHHEEECCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEHHHHHHHHH
DMFNPTVQLKSGGYIVINITEALVAIDVNSGRSTKEGSIEDTATKTNLEAAEEVARQVRL
HHCCCEEEEECCCEEEEEEECEEEEEECCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHH
RDLAGLIVIDFIDMDERRNNNAVEKRIKDKLKTDRARIQVGRISTFGLMEMSRQRLRPGM
HHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHCCCCH
LEATTQACHHCHGTGLVRSDDSLALSILRQLEEEGTRGRAREVLLTAPVGIVNFLINFKR
HHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCCCCHHEEEEECCHHHHHHHHHHHH
DYLDAIEARSGMAIRIEADASLVVPDYKIEKFKTATRKITAVAPVVSMDAGLMDDIDEDA
HHHHHHHHCCCCEEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCC
GDDVVEDADQVDEAAEASEDEQPRKKRRRRRGGRGRRRTSDENGETGDSAEDGSAAAAVP
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EEAAGADPSTDTPPEASPEGEEEQPKKRRRSRRGGRGRKSSEAQAETTDEGNGDAMEAQD
HHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APAAEPAEPASVSTPEPEVAADAEPEITPEPTPDPEPEPQPERELVAADASVASEEPAQA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCHHHCCCCCHHH
AEPTPEPEAAPEPTPEPVAAEATPEPVAAQAAAEPDAPAKPKRRGWWSRG
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]