Click here to switch to the map view.

The map label for this gene is trmFO

Identifier: 89054989

GI number: 89054989

Start: 2496542

End: 2497918

Strand: Direct

Name: trmFO

Synonym: Jann_2498

Alternate gene names: 89054989

Gene position: 2496542-2497918 (Clockwise)

Preceding gene: 89054988

Following gene: 89054990

Centisome position: 57.82

GC content: 63.76

Gene sequence:

>1377_bases
ATGACACAGAACACACAACTCACCATAATCGGCGGCGGCATGGCCGGGTCTGAGGCCGCCTGGCAGGCCGCCAACCTTGG
CGTCAACGTGCGCCTGATCGAGATGCGGCCCAAGGTCGAAACCTTCGCCCACCGCACCGGCAATCTGGCCGAGATGGTGT
GCTCCAACTCCTTCCGCTCCGACGACAGCGAGCAGAACGCCGTGGGCCTGTTGCATTGGGAAATGCGGGCGGCCAATTCC
GTGATCATGCACACCGCAGATTCCCACAAGCTGCCCGCAGGCGGCGCGCTCGCGGTGGATCGGGATCCGTTTGCAGAGGC
CGTCACGGCGAAACTACACGCCCATCCCAACATCGAAATCACCTATGGCGAGGTCACCGACCTCCCTGCCGCGGGCCCAA
CGATCATCGCCACCGGCCCCCTCACCGGCAGCGCTTTGGCCGATGCGATAGCCCGCGAAGCAGGCCAGGACGCGCTGGCG
TTTTTCGACGCCATCGCCCCCATCGTCTACGCCGACAGCATCGATATGGACATTGCCTGGCGACAATCCCGCTATGACAA
GGGCGACACGCTGGAGGAGCAGCAGGCCTATATCAATTGCCCTCTGACGCGCGATCAATACGAGGCCTTCATCGACGCGC
TCCTCTCTGCCGACAAGACCCAGTTCAAGGACGGCGAGACGGCTGGTTACTTCGACGGCTGCCTTCCCATCGAGGTCATG
GCCGAGCGGGGCCGTGAAACCCTGCGCTTTGGCCCGATGAAGCCCGTGGGCCTCACCAATCCCCATGACCCGCAGACCAA
GGCCTATGCCGTGGTCCAGCTACGCCGTGATAACGCTTTGGGAACGCTCTACAATATCGTGGGCTTCCAGACCAAGATGA
CCTATGGCGCGCAGAAGCAAGTCTTCGCGATGATCCCCGGCCTGCAGGAGGCCAGTTTCGCGCGTCTGGGTGGCATCCAC
CGCAACACCTTCATCAACTCGCCCACGCTGCTGGACGACCAGATGCGCCTTCGCTCCAAGCCACATATCCGGTTCGCGGG
CCAGATTACCGGCGTCGAAGGCTATGTGGAGAGCGCCTCCATGGGTCTTCTCGCAGGGCGCATGGCCGCTGCCGAGATCC
TGGGTGAGACGTTGCCAGACCTCCCCGACACGACCGCCATGGGCGCGCTCGTCACGCACATCACCGGCGGGGCGGACGCG
AAAACGTTTCAGCCGATGAACGTGAATTTCGGCCTGTTTCCCCCGGTGGAGGGCCTGAAGGGCGGCCGCCGGGGCCGCAA
GGACCGCTACAAGGCCTATACCGACCGCGCCAAAGCTGCGTGGACCAACTGGCTCACGCCCCAGACATCATTGGGCGGAT
CGCAAGCGGCGGAGTGA

Upstream 100 bases:

>100_bases
GCACCCAAGTCATCTGGGCTCACACCAGCCCCAAACACCAAGCATAGGAACAAAGCCTTCAGGCTTTTCCTTTTCATAAG
AAATCCCTACATCTCCCCCC

Downstream 100 bases:

>100_bases
TCTGGACGGCGCGCCTGTCGCCGCGTTAGCATCACCCCATGAAAAGCCCCATCGACGCAAAACTCTGGACGCCCCGCTCT
GTCGAAGACACGCAAGCCCT

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MTQNTQLTIIGGGMAGSEAAWQAANLGVNVRLIEMRPKVETFAHRTGNLAEMVCSNSFRSDDSEQNAVGLLHWEMRAANS
VIMHTADSHKLPAGGALAVDRDPFAEAVTAKLHAHPNIEITYGEVTDLPAAGPTIIATGPLTGSALADAIAREAGQDALA
FFDAIAPIVYADSIDMDIAWRQSRYDKGDTLEEQQAYINCPLTRDQYEAFIDALLSADKTQFKDGETAGYFDGCLPIEVM
AERGRETLRFGPMKPVGLTNPHDPQTKAYAVVQLRRDNALGTLYNIVGFQTKMTYGAQKQVFAMIPGLQEASFARLGGIH
RNTFINSPTLLDDQMRLRSKPHIRFAGQITGVEGYVESASMGLLAGRMAAAEILGETLPDLPDTTAMGALVTHITGGADA
KTFQPMNVNFGLFPPVEGLKGGRRGRKDRYKAYTDRAKAAWTNWLTPQTSLGGSQAAE

Sequences:

>Translated_458_residues
MTQNTQLTIIGGGMAGSEAAWQAANLGVNVRLIEMRPKVETFAHRTGNLAEMVCSNSFRSDDSEQNAVGLLHWEMRAANS
VIMHTADSHKLPAGGALAVDRDPFAEAVTAKLHAHPNIEITYGEVTDLPAAGPTIIATGPLTGSALADAIAREAGQDALA
FFDAIAPIVYADSIDMDIAWRQSRYDKGDTLEEQQAYINCPLTRDQYEAFIDALLSADKTQFKDGETAGYFDGCLPIEVM
AERGRETLRFGPMKPVGLTNPHDPQTKAYAVVQLRRDNALGTLYNIVGFQTKMTYGAQKQVFAMIPGLQEASFARLGGIH
RNTFINSPTLLDDQMRLRSKPHIRFAGQITGVEGYVESASMGLLAGRMAAAEILGETLPDLPDTTAMGALVTHITGGADA
KTFQPMNVNFGLFPPVEGLKGGRRGRKDRYKAYTDRAKAAWTNWLTPQTSLGGSQAAE
>Mature_457_residues
TQNTQLTIIGGGMAGSEAAWQAANLGVNVRLIEMRPKVETFAHRTGNLAEMVCSNSFRSDDSEQNAVGLLHWEMRAANSV
IMHTADSHKLPAGGALAVDRDPFAEAVTAKLHAHPNIEITYGEVTDLPAAGPTIIATGPLTGSALADAIAREAGQDALAF
FDAIAPIVYADSIDMDIAWRQSRYDKGDTLEEQQAYINCPLTRDQYEAFIDALLSADKTQFKDGETAGYFDGCLPIEVMA
ERGRETLRFGPMKPVGLTNPHDPQTKAYAVVQLRRDNALGTLYNIVGFQTKMTYGAQKQVFAMIPGLQEASFARLGGIHR
NTFINSPTLLDDQMRLRSKPHIRFAGQITGVEGYVESASMGLLAGRMAAAEILGETLPDLPDTTAMGALVTHITGGADAK
TFQPMNVNFGLFPPVEGLKGGRRGRKDRYKAYTDRAKAAWTNWLTPQTSLGGSQAAE

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_JANSC (Q28PE7)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_510440.1
- ProteinModelPortal:   Q28PE7
- SMR:   Q28PE7
- STRING:   Q28PE7
- GeneID:   3934957
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_2498
- eggNOG:   COG1206
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- BioCyc:   JSP290400:JANN_2498-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 49480; Mature: 49348

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQNTQLTIIGGGMAGSEAAWQAANLGVNVRLIEMRPKVETFAHRTGNLAEMVCSNSFRS
CCCCCEEEEEECCCCCCHHHHHHHCCCEEEEEEEECCCHHHHHHCCCCHHHHHHCCCCCC
DDSEQNAVGLLHWEMRAANSVIMHTADSHKLPAGGALAVDRDPFAEAVTAKLHAHPNIEI
CCCCCCEEEEEEEEHHCCCEEEEEECCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCEEE
TYGEVTDLPAAGPTIIATGPLTGSALADAIAREAGQDALAFFDAIAPIVYADSIDMDIAW
EECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHEECCCCCEEEE
RQSRYDKGDTLEEQQAYINCPLTRDQYEAFIDALLSADKTQFKDGETAGYFDGCLPIEVM
HHHCCCCCCCCCCCHHEEECCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCHHHHH
AERGRETLRFGPMKPVGLTNPHDPQTKAYAVVQLRRDNALGTLYNIVGFQTKMTYGAQKQ
HHCCCHHEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHEEECCCCCHH
VFAMIPGLQEASFARLGGIHRNTFINSPTLLDDQMRLRSKPHIRFAGQITGVEGYVESAS
HHEECCCCCHHHHHHHCCCHHHCCCCCCCCCCHHHHHCCCCCEEEEEEECCCHHHHHHHH
MGLLAGRMAAAEILGETLPDLPDTTAMGALVTHITGGADAKTFQPMNVNFGLFPPVEGLK
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCHHHCC
GGRRGRKDRYKAYTDRAKAAWTNWLTPQTSLGGSQAAE
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TQNTQLTIIGGGMAGSEAAWQAANLGVNVRLIEMRPKVETFAHRTGNLAEMVCSNSFRS
CCCCEEEEEECCCCCCHHHHHHHCCCEEEEEEEECCCHHHHHHCCCCHHHHHHCCCCCC
DDSEQNAVGLLHWEMRAANSVIMHTADSHKLPAGGALAVDRDPFAEAVTAKLHAHPNIEI
CCCCCCEEEEEEEEHHCCCEEEEEECCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCEEE
TYGEVTDLPAAGPTIIATGPLTGSALADAIAREAGQDALAFFDAIAPIVYADSIDMDIAW
EECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHEECCCCCEEEE
RQSRYDKGDTLEEQQAYINCPLTRDQYEAFIDALLSADKTQFKDGETAGYFDGCLPIEVM
HHHCCCCCCCCCCCHHEEECCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCHHHHH
AERGRETLRFGPMKPVGLTNPHDPQTKAYAVVQLRRDNALGTLYNIVGFQTKMTYGAQKQ
HHCCCHHEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHEEECCCCCHH
VFAMIPGLQEASFARLGGIHRNTFINSPTLLDDQMRLRSKPHIRFAGQITGVEGYVESAS
HHEECCCCCHHHHHHHCCCHHHCCCCCCCCCCHHHHHCCCCCEEEEEEECCCHHHHHHHH
MGLLAGRMAAAEILGETLPDLPDTTAMGALVTHITGGADAKTFQPMNVNFGLFPPVEGLK
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCHHHCC
GGRRGRKDRYKAYTDRAKAAWTNWLTPQTSLGGSQAAE
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA