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The map label for this gene is fabI1 [H]

Identifier: 89054967

GI number: 89054967

Start: 2473394

End: 2474206

Strand: Direct

Name: fabI1 [H]

Synonym: Jann_2476

Alternate gene names: 89054967

Gene position: 2473394-2474206 (Clockwise)

Preceding gene: 89054958

Following gene: 89054968

Centisome position: 57.28

GC content: 60.27

Gene sequence:

>813_bases
ATGATGGGTATGATGGATGGCAAGCGCGGACTGATCATGGGCCTCGCCAACGATAAGTCCATCGCCTGGGGCATCGCGAA
AGCGGTCGCGGCCGAGGGCGCGGAGCTGGCCTTTTCATATCAGGGCGACGCCCTTCTCAAGCGCGTGGGGCCGTTGGCAA
AGCAGGTGGGCTCTGACCTGGTCATCCCCTGCGATGTCGGGGATGAGGCCTCCCTCGATGCGCTGTTCGAGACGCTGGAG
GCGGAATGGGGCAACATTGACTTCGTCGTCCACGCCATTGGATTCTCGGACAAAAATGAACTTCGCGGCCGCTATGTGGA
GACCTCGCCCGCGAATTTTGAGATGACGATGAACATCTCCGTCTATTCCTTCACCGCCGTGTGCCAGCGGGCGGAAAAGA
TGATGAACAATGGCGGCTCGCTCCTCACGCTGACCTATTACGGGTCCGAGAAGGTCATGCCCCATTACAACGTCATGGGC
GTGGCCAAGGCAGCCCTTGAGGCGTCGGTGATGTATCTGGCCGAAGATCTGGGCCGCGACGGTATCCGGGTGAACGCGAT
CAGCGCGGGCACGATCAAGACGCTGGCCGCCAGCGGCATCGGCGATTTCCGCTACATCCTGAAGTGGAATGAGAACAACT
CACCACTGCGGCGCACCGTCACCCAGGATGAGGTCGGCAAATCCGCGCTCTACCTGCTGTCCGATCTGGGCAGCGCCGTG
ACGGGAGAGGTGCACCATGTGGATGCGGGCTACCACGTCATCGGCATGAAAAACCCCGAGGCCCCGGATATGTCTTTGGA
AAAGAAGGACTAA

Upstream 100 bases:

>100_bases
AGCGGGCCACACCGGGCACGGCAAGGGCTGGCTTAATCTTGAAGCCCTGCGGGGGATGGCCTACGAGAGAGGCAACAACA
CACCAGGTAAGTGGGACGAT

Downstream 100 bases:

>100_bases
GCCATGACCACGCAACTCCCCCACGAAAAAGGCTTTCATATCTCCTGGGATCAGATCCACCGTGACAGCCGCGCCTTGGC
GTGGCGGCTGGACAATCACG

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 1 [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MMGMMDGKRGLIMGLANDKSIAWGIAKAVAAEGAELAFSYQGDALLKRVGPLAKQVGSDLVIPCDVGDEASLDALFETLE
AEWGNIDFVVHAIGFSDKNELRGRYVETSPANFEMTMNISVYSFTAVCQRAEKMMNNGGSLLTLTYYGSEKVMPHYNVMG
VAKAALEASVMYLAEDLGRDGIRVNAISAGTIKTLAASGIGDFRYILKWNENNSPLRRTVTQDEVGKSALYLLSDLGSAV
TGEVHHVDAGYHVIGMKNPEAPDMSLEKKD

Sequences:

>Translated_270_residues
MMGMMDGKRGLIMGLANDKSIAWGIAKAVAAEGAELAFSYQGDALLKRVGPLAKQVGSDLVIPCDVGDEASLDALFETLE
AEWGNIDFVVHAIGFSDKNELRGRYVETSPANFEMTMNISVYSFTAVCQRAEKMMNNGGSLLTLTYYGSEKVMPHYNVMG
VAKAALEASVMYLAEDLGRDGIRVNAISAGTIKTLAASGIGDFRYILKWNENNSPLRRTVTQDEVGKSALYLLSDLGSAV
TGEVHHVDAGYHVIGMKNPEAPDMSLEKKD
>Mature_270_residues
MMGMMDGKRGLIMGLANDKSIAWGIAKAVAAEGAELAFSYQGDALLKRVGPLAKQVGSDLVIPCDVGDEASLDALFETLE
AEWGNIDFVVHAIGFSDKNELRGRYVETSPANFEMTMNISVYSFTAVCQRAEKMMNNGGSLLTLTYYGSEKVMPHYNVMG
VAKAALEASVMYLAEDLGRDGIRVNAISAGTIKTLAASGIGDFRYILKWNENNSPLRRTVTQDEVGKSALYLLSDLGSAV
TGEVHHVDAGYHVIGMKNPEAPDMSLEKKD

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=260, Percent_Identity=50.3846153846154, Blast_Score=261, Evalue=5e-71,
Organism=Escherichia coli, GI1789378, Length=256, Percent_Identity=26.5625, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1787905, Length=249, Percent_Identity=28.1124497991968, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082100, Length=258, Percent_Identity=27.5193798449612, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI2367365, Length=254, Percent_Identity=25.9842519685039, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 29143; Mature: 29143

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMGMMDGKRGLIMGLANDKSIAWGIAKAVAAEGAELAFSYQGDALLKRVGPLAKQVGSDL
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHCCCE
VIPCDVGDEASLDALFETLEAEWGNIDFVVHAIGFSDKNELRGRYVETSPANFEMTMNIS
EEEECCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHCCEEEECCCCCEEEEEEEE
VYSFTAVCQRAEKMMNNGGSLLTLTYYGSEKVMPHYNVMGVAKAALEASVMYLAEDLGRD
EHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
GIRVNAISAGTIKTLAASGIGDFRYILKWNENNSPLRRTVTQDEVGKSALYLLSDLGSAV
CEEEEEEECCHHHHHHHCCCCCEEEEEEECCCCCHHHHHCCHHHHCHHHHHHHHHCCHHH
TGEVHHVDAGYHVIGMKNPEAPDMSLEKKD
CCCEEEECCCEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MMGMMDGKRGLIMGLANDKSIAWGIAKAVAAEGAELAFSYQGDALLKRVGPLAKQVGSDL
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHCCCE
VIPCDVGDEASLDALFETLEAEWGNIDFVVHAIGFSDKNELRGRYVETSPANFEMTMNIS
EEEECCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHCCEEEECCCCCEEEEEEEE
VYSFTAVCQRAEKMMNNGGSLLTLTYYGSEKVMPHYNVMGVAKAALEASVMYLAEDLGRD
EHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
GIRVNAISAGTIKTLAASGIGDFRYILKWNENNSPLRRTVTQDEVGKSALYLLSDLGSAV
CEEEEEEECCHHHHHHHCCCCCEEEEEEECCCCCHHHHHCCHHHHCHHHHHHHHHCCHHH
TGEVHHVDAGYHVIGMKNPEAPDMSLEKKD
CCCEEEECCCEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]