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The map label for this gene is trmJ [H]

Identifier: 89054924

GI number: 89054924

Start: 2427327

End: 2428103

Strand: Reverse

Name: trmJ [H]

Synonym: Jann_2433

Alternate gene names: 89054924

Gene position: 2428103-2427327 (Counterclockwise)

Preceding gene: 89054926

Following gene: 89054923

Centisome position: 56.23

GC content: 64.74

Gene sequence:

>777_bases
ATGTCAGATGCCACGCCCCCGTCAGTCACCCCAGGCGGACAACCCGCCTTCGTTCTGGTCCGCCCGCAGATGGGGGAAAA
CATCGGTGCGGCTGCGCGCGCGATGTGGAACTTCGGCCTCGACCGGATGCGCGTGGTAGACCCCCGCGATGGCTGGCCCA
ATGAACGCGCTGTCGCGCTGGCCAGCGGCGCCGGGCGGTTGCTGGACAATGCGGGCCTGTACCAGGATCTGCCCGGGGCC
TTGAGCGATTGCACCTACGTCTTTGCGACTACGGCCCGGGACCGGGGCCTGACCAAGCCTATCGTGACGCCCGAGCGCGC
GATGGAGCAGACCCGCGCGATCTTGGCCGAGGGAGGTAAGGTGGGCGTGCTGTTCGGGCCGGAGCGGGCGGGCCTTGAGA
ACGCAGATATCGCGCCTGCCGATGCGATTATATCTGTCGATGTGAACCCGGCATTTCCATCCTTGAATTTGGCGCAATGT
GTGCTGCTGTGTGCCTATGAGTGGCAGCGGCAGGGCTCTACCGTCGCGCCCGAGGTCATGGCGCTGGCCAAGACAGATTT
TGCCAGCAATCTCGAAGTAGAAAAGCTCGGCGCGCATTACGAGGAGCGGTTGGAGGAGGCGGGGTTCTTCTGGCCGCCCG
CGAAAGCCGACAGTATGAAACTGACGTTGCGCAACCTGTGGGGGCGGTTGAACCTGACGCGGGCGGACGTGCAAACCCTG
CATGGGGTGATGCGGCAGATGGTGCGGTGGGCCGAGGGGCGGCGCAAGGATTTGTAA

Upstream 100 bases:

>100_bases
CTGGCGGCAAAGGGTCAGTGCGGCGCTGATACCGTCCCTTTGCGGCCAAGGCAATCGGCGCACATCTTGCGCCGTCACGC
GCCGCTGGGTATGGGCAGGC

Downstream 100 bases:

>100_bases
GGTGCGGCGCTTGGCTGGCTTCTGACTTGGGGCTCTGCCCCAAACCCCGGGATATTTCAGGAACGGGGAAATGGGGAGTG
CACCACTGGGTTGAGCGCGC

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSDATPPSVTPGGQPAFVLVRPQMGENIGAAARAMWNFGLDRMRVVDPRDGWPNERAVALASGAGRLLDNAGLYQDLPGA
LSDCTYVFATTARDRGLTKPIVTPERAMEQTRAILAEGGKVGVLFGPERAGLENADIAPADAIISVDVNPAFPSLNLAQC
VLLCAYEWQRQGSTVAPEVMALAKTDFASNLEVEKLGAHYEERLEEAGFFWPPAKADSMKLTLRNLWGRLNLTRADVQTL
HGVMRQMVRWAEGRRKDL

Sequences:

>Translated_258_residues
MSDATPPSVTPGGQPAFVLVRPQMGENIGAAARAMWNFGLDRMRVVDPRDGWPNERAVALASGAGRLLDNAGLYQDLPGA
LSDCTYVFATTARDRGLTKPIVTPERAMEQTRAILAEGGKVGVLFGPERAGLENADIAPADAIISVDVNPAFPSLNLAQC
VLLCAYEWQRQGSTVAPEVMALAKTDFASNLEVEKLGAHYEERLEEAGFFWPPAKADSMKLTLRNLWGRLNLTRADVQTL
HGVMRQMVRWAEGRRKDL
>Mature_257_residues
SDATPPSVTPGGQPAFVLVRPQMGENIGAAARAMWNFGLDRMRVVDPRDGWPNERAVALASGAGRLLDNAGLYQDLPGAL
SDCTYVFATTARDRGLTKPIVTPERAMEQTRAILAEGGKVGVLFGPERAGLENADIAPADAIISVDVNPAFPSLNLAQCV
LLCAYEWQRQGSTVAPEVMALAKTDFASNLEVEKLGAHYEERLEEAGFFWPPAKADSMKLTLRNLWGRLNLTRADVQTLH
GVMRQMVRWAEGRRKDL

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=248, Percent_Identity=30.241935483871, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1790865, Length=152, Percent_Identity=35.5263157894737, Blast_Score=88, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28103; Mature: 27972

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDATPPSVTPGGQPAFVLVRPQMGENIGAAARAMWNFGLDRMRVVDPRDGWPNERAVAL
CCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEE
ASGAGRLLDNAGLYQDLPGALSDCTYVFATTARDRGLTKPIVTPERAMEQTRAILAEGGK
ECCCCHHHHCCCCHHHCCCHHHHCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCE
VGVLFGPERAGLENADIAPADAIISVDVNPAFPSLNLAQCVLLCAYEWQRQGSTVAPEVM
EEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHH
ALAKTDFASNLEVEKLGAHYEERLEEAGFFWPPAKADSMKLTLRNLWGRLNLTRADVQTL
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHEEHHHHHHCCCCCCHHHHHHH
HGVMRQMVRWAEGRRKDL
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDATPPSVTPGGQPAFVLVRPQMGENIGAAARAMWNFGLDRMRVVDPRDGWPNERAVAL
CCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEE
ASGAGRLLDNAGLYQDLPGALSDCTYVFATTARDRGLTKPIVTPERAMEQTRAILAEGGK
ECCCCHHHHCCCCHHHCCCHHHHCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCE
VGVLFGPERAGLENADIAPADAIISVDVNPAFPSLNLAQCVLLCAYEWQRQGSTVAPEVM
EEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHH
ALAKTDFASNLEVEKLGAHYEERLEEAGFFWPPAKADSMKLTLRNLWGRLNLTRADVQTL
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHEEHHHHHHCCCCCCHHHHHHH
HGVMRQMVRWAEGRRKDL
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]