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The map label for this gene is paaF [C]

Identifier: 89054921

GI number: 89054921

Start: 2423690

End: 2424478

Strand: Direct

Name: paaF [C]

Synonym: Jann_2430

Alternate gene names: 89054921

Gene position: 2423690-2424478 (Clockwise)

Preceding gene: 89054919

Following gene: 89054925

Centisome position: 56.13

GC content: 64.77

Gene sequence:

>789_bases
ATGCCTGAGCCCCTCATCCTCCGCGAAGATCGGGATGCAATCACCCATCTGACCCTGAATTCGCCGGGCAATCTGAACGC
GCTCTCCAATGAGATGCTGGCCGCGCTGCAAGGGGCGCTGGATACGCTGGCGCAAGAGCCGTCCCAGAAGGTCATCGTCC
TGAAAGGCGCGGGCCGGGTGTTCTGCGCCGGCCATGATCTCAAGCAAATGCAGGCGGCCAGGCAAGCCGAAGACAACGGG
GCCGCCGCCTGGAAAGACCTCTTTGATCGCTGCTCAACTCTGATGCAGACGATCCCCCGCCTGCCCCAGCCGGTCATTGC
CCAGGTCCACGGGCTGGCGGCGGCGGCGGGCTGCCAATTGGTCGCCTCCTGCGATCTGGCCGTGGCCGAGACGACGGCAA
AGTTCGGCGTCAACGGGGTAAATATCGGCCTCTTCTGCTCCACGCCCATGGTGGCGCTGTCCCGCGTCATGCCACGAAAA
CAGGTGTTTGAAATGCTGACGACCGGAGAATTCCTGGATGCCCCCCGGGCCCGGGACGTGGGCCTGATCAACCGCATCGC
CAGCGCCGACACGCTGGACGACGCCACGCAAGGCCTGGCCGAGGTCATCGCCGCCAAGCTCTCCTCCGCCGTCCGCGTCG
GCAAGCAAGCCTTCTACGAGCAGGCCGATCTGACGCTGGCCCAGGCCTACGCGCTCACCGGCGATGTCATGGTCGAGAAC
ATGCTGAACGCCCAGACCAGCGAGGGCATCACTGCCTTCCTGGAGAAACGCGCACCCGATTGGTCATGA

Upstream 100 bases:

>100_bases
GCATCTTTATGTCCACACCACTTTTCCCTTCGCTCGATCACCGCTACCGTTCCCCGAAGTGCCATTTGCATGCAAGGCCC
CAGACACAAGGATGCCACCG

Downstream 100 bases:

>100_bases
TCCCCCCCTCGCCGCTGCGAGGAGGGCGGGCACCGCCCGAGGAGGATCCCGCCCCGAAGGGACCTCTCAGACCCCGAACA
ATGCGCCGTATTTCGCATCC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MPEPLILREDRDAITHLTLNSPGNLNALSNEMLAALQGALDTLAQEPSQKVIVLKGAGRVFCAGHDLKQMQAARQAEDNG
AAAWKDLFDRCSTLMQTIPRLPQPVIAQVHGLAAAAGCQLVASCDLAVAETTAKFGVNGVNIGLFCSTPMVALSRVMPRK
QVFEMLTTGEFLDAPRARDVGLINRIASADTLDDATQGLAEVIAAKLSSAVRVGKQAFYEQADLTLAQAYALTGDVMVEN
MLNAQTSEGITAFLEKRAPDWS

Sequences:

>Translated_262_residues
MPEPLILREDRDAITHLTLNSPGNLNALSNEMLAALQGALDTLAQEPSQKVIVLKGAGRVFCAGHDLKQMQAARQAEDNG
AAAWKDLFDRCSTLMQTIPRLPQPVIAQVHGLAAAAGCQLVASCDLAVAETTAKFGVNGVNIGLFCSTPMVALSRVMPRK
QVFEMLTTGEFLDAPRARDVGLINRIASADTLDDATQGLAEVIAAKLSSAVRVGKQAFYEQADLTLAQAYALTGDVMVEN
MLNAQTSEGITAFLEKRAPDWS
>Mature_261_residues
PEPLILREDRDAITHLTLNSPGNLNALSNEMLAALQGALDTLAQEPSQKVIVLKGAGRVFCAGHDLKQMQAARQAEDNGA
AAWKDLFDRCSTLMQTIPRLPQPVIAQVHGLAAAAGCQLVASCDLAVAETTAKFGVNGVNIGLFCSTPMVALSRVMPRKQ
VFEMLTTGEFLDAPRARDVGLINRIASADTLDDATQGLAEVIAAKLSSAVRVGKQAFYEQADLTLAQAYALTGDVMVENM
LNAQTSEGITAFLEKRAPDWS

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=261, Percent_Identity=44.0613026819923, Blast_Score=200, Evalue=1e-51,
Organism=Homo sapiens, GI194097323, Length=259, Percent_Identity=28.5714285714286, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI70995211, Length=235, Percent_Identity=27.2340425531915, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI4502327, Length=264, Percent_Identity=26.1363636363636, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI157694516, Length=231, Percent_Identity=25.974025974026, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI213417737, Length=231, Percent_Identity=25.974025974026, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=263, Percent_Identity=32.319391634981, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=31.2977099236641, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI87082183, Length=255, Percent_Identity=27.0588235294118, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788597, Length=262, Percent_Identity=27.0992366412214, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI221142681, Length=255, Percent_Identity=27.0588235294118, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1790281, Length=185, Percent_Identity=24.8648648648649, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17540306, Length=250, Percent_Identity=36.4, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17554946, Length=263, Percent_Identity=28.5171102661597, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI25145438, Length=251, Percent_Identity=30.2788844621514, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17540714, Length=230, Percent_Identity=29.1304347826087, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560910, Length=245, Percent_Identity=25.7142857142857, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=146, Percent_Identity=28.0821917808219, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=227, Percent_Identity=24.2290748898678, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17549921, Length=222, Percent_Identity=23.8738738738739, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17535521, Length=261, Percent_Identity=22.9885057471264, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19922422, Length=260, Percent_Identity=35.7692307692308, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI20129971, Length=250, Percent_Identity=29.2, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24653477, Length=250, Percent_Identity=29.2, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24653139, Length=268, Percent_Identity=26.4925373134328, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI19920382, Length=230, Percent_Identity=28.695652173913, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21357171, Length=273, Percent_Identity=25.6410256410256, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24650670, Length=279, Percent_Identity=24.3727598566308, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24583165, Length=218, Percent_Identity=26.1467889908257, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27936; Mature: 27805

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEPLILREDRDAITHLTLNSPGNLNALSNEMLAALQGALDTLAQEPSQKVIVLKGAGRV
CCCCCEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEE
FCAGHDLKQMQAARQAEDNGAAAWKDLFDRCSTLMQTIPRLPQPVIAQVHGLAAAAGCQL
EECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VASCDLAVAETTAKFGVNGVNIGLFCSTPMVALSRVMPRKQVFEMLTTGEFLDAPRARDV
HHHCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHCCHHHHHHHHHCCHHHCCCCCCCH
GLINRIASADTLDDATQGLAEVIAAKLSSAVRVGKQAFYEQADLTLAQAYALTGDVMVEN
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLNAQTSEGITAFLEKRAPDWS
HHHCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PEPLILREDRDAITHLTLNSPGNLNALSNEMLAALQGALDTLAQEPSQKVIVLKGAGRV
CCCCEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEE
FCAGHDLKQMQAARQAEDNGAAAWKDLFDRCSTLMQTIPRLPQPVIAQVHGLAAAAGCQL
EECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VASCDLAVAETTAKFGVNGVNIGLFCSTPMVALSRVMPRKQVFEMLTTGEFLDAPRARDV
HHHCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHCCHHHHHHHHHCCHHHCCCCCCCH
GLINRIASADTLDDATQGLAEVIAAKLSSAVRVGKQAFYEQADLTLAQAYALTGDVMVEN
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLNAQTSEGITAFLEKRAPDWS
HHHCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA