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The map label for this gene is lcfB [H]

Identifier: 89054806

GI number: 89054806

Start: 2319221

End: 2320657

Strand: Direct

Name: lcfB [H]

Synonym: Jann_2315

Alternate gene names: 89054806

Gene position: 2319221-2320657 (Clockwise)

Preceding gene: 89054804

Following gene: 89054807

Centisome position: 53.71

GC content: 65.97

Gene sequence:

>1437_bases
ATGGACTGCCCCACCCGCTTCAACCTGTCGACCTATGTGCTGGCCAAGGCCGAGGCCACGCCTGACAAGATCGCGCTTGC
GATCCTGGGTCCGTCGCGGGCCGAGCGGTGGTCCTATCGCAGGCTGGAAGATGCCGTCGCTCGCGCTGCGGGCGGGCTGG
TGAAAAGTGGTTTAAGATCGCAGGATAAGCTATTGCTAAGGCTCGGCAATTCTGCGCGTTTTCCCATCGCATATCTGGGC
GCGATCCGGGCGGGCATCGTGCCCATCCCGACCTCTGCCGCGCTGACCCCGCCGGAGGTCACGACGCTGGTCCAGGCCCT
GCCTCCGACGGCCATATTGGCGGAGCCCGGCGTGGCCCTGCCAGATGCGGCCTGCCCCATATTGCCCCTTGAAACACTGG
ACGGTGACCCGCTGCCACCCGTCGACACCGACGCGGACGATCTGGCCTATATCGTGTTCACCTCCGGCTCCTCCGGCCTG
CCCAAGGCGGTGGCCCATGCCCACCGGGCGGTCTGGGCGCGGCGCATGATGTGGGACGGGTGGTACGGCCTGCGCGCGGA
TGACAGGCTGATGCATACGGGCGCGTTCAACTGGACTTACACCCTTGGCACCGGGCTTTTGGACCCCTGGGCGATTGGGG
CGACTGCGCTGGTGCCAGCCCCCGGAACGGACCCTTCGAGCCTTGCCCTGCTGGCCAAACGCCATGATGCGAGCATTCTG
GCCGGCAGCCCCGGTATCTTCCGCAAGCTGCTCCGCGGCCCTCTGCCACCCCTGCCCAAACTCCGCCACGCGCTCAGCGC
CGGGGAAGCTCTGCCGCCCTCCCTTCGGACCCGGTGGCAGGAGGCAACGGGCACCGATATCCACGAGGCATTGGGCATGT
CCGAGGTCTCGACCTTCCTGTCCGGCTCGCCCGCGCGGCCCGCGCCCGATGGCACGACCGGATATCCGCAGGATGGGCGA
AAGATCACCGTCCGCAACGGGTGCCTTGCGGTCCACAAAAGGGACCCCGGACTGATGCTGGGGTATATCAATGGTAGATC
AATTGATCTTCCGCTCTCCGATGGCTGGTTTGAAACCTCCGATCTGGTGACCCAAAGTGACGACGGGGCGTACACCTATC
TGGGCCGCGCCGATGATGTTCTGACGGCGGGCGGGTTCCGTATCGCGCCGCTGGAGATTGAGCAGGTCTTCGACGGGCTC
GATGGCGTCACCGAATGCGCCGCCCTCACTTTGCATCCGACCTCCGAGACGACCATTCTCGCCCTTGCCTATTGCGGCAC
CGCGCGCGAGGATCAGCTCACGCAGCACGCCACCACCCACCTCGCCCGCCACAAACATCCCCGCGCCTATCTGCATCTGC
CAGATGGCCTGCCCCGGGGCGCGAATGGAAAATTGAACCGCCGCGCGCTGGCGCAGGCGGTGAAGGACATGACATGA

Upstream 100 bases:

>100_bases
GGATCAGGCTGGGTTGATCGGGGGCGTGGCTCATGCACCGTGCGATACCGCACACCCTGCCCGCCTTGCAACCTCCGCCG
CGCTCTCTTAGGCCATTTCC

Downstream 100 bases:

>100_bases
TCAAACTCGACATCCTCTCGGACCCTATTTGCCCCTGGTGCTATATCGGCTGGTCGAATCTGGCCCGTGCGATGGAGGCG
AGGCCGAACCACCCCTTCAC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MDCPTRFNLSTYVLAKAEATPDKIALAILGPSRAERWSYRRLEDAVARAAGGLVKSGLRSQDKLLLRLGNSARFPIAYLG
AIRAGIVPIPTSAALTPPEVTTLVQALPPTAILAEPGVALPDAACPILPLETLDGDPLPPVDTDADDLAYIVFTSGSSGL
PKAVAHAHRAVWARRMMWDGWYGLRADDRLMHTGAFNWTYTLGTGLLDPWAIGATALVPAPGTDPSSLALLAKRHDASIL
AGSPGIFRKLLRGPLPPLPKLRHALSAGEALPPSLRTRWQEATGTDIHEALGMSEVSTFLSGSPARPAPDGTTGYPQDGR
KITVRNGCLAVHKRDPGLMLGYINGRSIDLPLSDGWFETSDLVTQSDDGAYTYLGRADDVLTAGGFRIAPLEIEQVFDGL
DGVTECAALTLHPTSETTILALAYCGTAREDQLTQHATTHLARHKHPRAYLHLPDGLPRGANGKLNRRALAQAVKDMT

Sequences:

>Translated_478_residues
MDCPTRFNLSTYVLAKAEATPDKIALAILGPSRAERWSYRRLEDAVARAAGGLVKSGLRSQDKLLLRLGNSARFPIAYLG
AIRAGIVPIPTSAALTPPEVTTLVQALPPTAILAEPGVALPDAACPILPLETLDGDPLPPVDTDADDLAYIVFTSGSSGL
PKAVAHAHRAVWARRMMWDGWYGLRADDRLMHTGAFNWTYTLGTGLLDPWAIGATALVPAPGTDPSSLALLAKRHDASIL
AGSPGIFRKLLRGPLPPLPKLRHALSAGEALPPSLRTRWQEATGTDIHEALGMSEVSTFLSGSPARPAPDGTTGYPQDGR
KITVRNGCLAVHKRDPGLMLGYINGRSIDLPLSDGWFETSDLVTQSDDGAYTYLGRADDVLTAGGFRIAPLEIEQVFDGL
DGVTECAALTLHPTSETTILALAYCGTAREDQLTQHATTHLARHKHPRAYLHLPDGLPRGANGKLNRRALAQAVKDMT
>Mature_478_residues
MDCPTRFNLSTYVLAKAEATPDKIALAILGPSRAERWSYRRLEDAVARAAGGLVKSGLRSQDKLLLRLGNSARFPIAYLG
AIRAGIVPIPTSAALTPPEVTTLVQALPPTAILAEPGVALPDAACPILPLETLDGDPLPPVDTDADDLAYIVFTSGSSGL
PKAVAHAHRAVWARRMMWDGWYGLRADDRLMHTGAFNWTYTLGTGLLDPWAIGATALVPAPGTDPSSLALLAKRHDASIL
AGSPGIFRKLLRGPLPPLPKLRHALSAGEALPPSLRTRWQEATGTDIHEALGMSEVSTFLSGSPARPAPDGTTGYPQDGR
KITVRNGCLAVHKRDPGLMLGYINGRSIDLPLSDGWFETSDLVTQSDDGAYTYLGRADDVLTAGGFRIAPLEIEQVFDGL
DGVTECAALTLHPTSETTILALAYCGTAREDQLTQHATTHLARHKHPRAYLHLPDGLPRGANGKLNRRALAQAVKDMT

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI58082049, Length=527, Percent_Identity=24.6679316888046, Blast_Score=149, Evalue=8e-36,
Organism=Homo sapiens, GI157311624, Length=527, Percent_Identity=24.8576850094877, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI157311622, Length=527, Percent_Identity=24.8576850094877, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI42544132, Length=528, Percent_Identity=23.6742424242424, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI38505220, Length=528, Percent_Identity=25.1893939393939, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI122937307, Length=525, Percent_Identity=23.4285714285714, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI115511026, Length=534, Percent_Identity=23.2209737827715, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI42544134, Length=356, Percent_Identity=23.876404494382, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI145693145, Length=519, Percent_Identity=21.9653179190751, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786801, Length=438, Percent_Identity=24.4292237442922, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789201, Length=325, Percent_Identity=26.1538461538462, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788107, Length=347, Percent_Identity=24.7838616714697, Blast_Score=79, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17558820, Length=451, Percent_Identity=22.8381374722838, Blast_Score=92, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI32563687, Length=446, Percent_Identity=22.1973094170404, Blast_Score=85, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=514, Percent_Identity=21.2062256809339, Blast_Score=80, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 51010; Mature: 51010

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDCPTRFNLSTYVLAKAEATPDKIALAILGPSRAERWSYRRLEDAVARAAGGLVKSGLRS
CCCCCCCCCEEEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QDKLLLRLGNSARFPIAYLGAIRAGIVPIPTSAALTPPEVTTLVQALPPTAILAEPGVAL
CCEEEEEECCCCCCHHHHHHHHHHCCEECCCCCCCCCHHHHHHHHHCCCCEEEECCCCCC
PDAACPILPLETLDGDPLPPVDTDADDLAYIVFTSGSSGLPKAVAHAHRAVWARRMMWDG
CCCCCCEEEHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCC
WYGLRADDRLMHTGAFNWTYTLGTGLLDPWAIGATALVPAPGTDPSSLALLAKRHDASIL
CCCCCCCCCEEECCCCEEEEECCCCCCCCHHCCCEEEECCCCCCHHHHHHHHHCCCCEEE
AGSPGIFRKLLRGPLPPLPKLRHALSAGEALPPSLRTRWQEATGTDIHEALGMSEVSTFL
ECCCHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHCHHHHHHHH
SGSPARPAPDGTTGYPQDGRKITVRNGCLAVHKRDPGLMLGYINGRSIDLPLSDGWFETS
CCCCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCCEEEEEECCCEEECCCCCCCCCCC
DLVTQSDDGAYTYLGRADDVLTAGGFRIAPLEIEQVFDGLDGVTECAALTLHPTSETTIL
CCEEECCCCCEEEECCCCCEEECCCEEEEEEEHHHHHCCCCCHHHHEEEEECCCCCCEEE
ALAYCGTAREDQLTQHATTHLARHKHPRAYLHLPDGLPRGANGKLNRRALAQAVKDMT
EEEECCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MDCPTRFNLSTYVLAKAEATPDKIALAILGPSRAERWSYRRLEDAVARAAGGLVKSGLRS
CCCCCCCCCEEEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QDKLLLRLGNSARFPIAYLGAIRAGIVPIPTSAALTPPEVTTLVQALPPTAILAEPGVAL
CCEEEEEECCCCCCHHHHHHHHHHCCEECCCCCCCCCHHHHHHHHHCCCCEEEECCCCCC
PDAACPILPLETLDGDPLPPVDTDADDLAYIVFTSGSSGLPKAVAHAHRAVWARRMMWDG
CCCCCCEEEHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCC
WYGLRADDRLMHTGAFNWTYTLGTGLLDPWAIGATALVPAPGTDPSSLALLAKRHDASIL
CCCCCCCCCEEECCCCEEEEECCCCCCCCHHCCCEEEECCCCCCHHHHHHHHHCCCCEEE
AGSPGIFRKLLRGPLPPLPKLRHALSAGEALPPSLRTRWQEATGTDIHEALGMSEVSTFL
ECCCHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHCHHHHHHHH
SGSPARPAPDGTTGYPQDGRKITVRNGCLAVHKRDPGLMLGYINGRSIDLPLSDGWFETS
CCCCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCCEEEEEECCCEEECCCCCCCCCCC
DLVTQSDDGAYTYLGRADDVLTAGGFRIAPLEIEQVFDGLDGVTECAALTLHPTSETTIL
CCEEECCCCCEEEECCCCCEEECCCEEEEEEEHHHHHCCCCCHHHHEEEEECCCCCCEEE
ALAYCGTAREDQLTQHATTHLARHKHPRAYLHLPDGLPRGANGKLNRRALAQAVKDMT
EEEECCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]