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The map label for this gene is psuK [H]

Identifier: 89054798

GI number: 89054798

Start: 2311027

End: 2311917

Strand: Direct

Name: psuK [H]

Synonym: Jann_2307

Alternate gene names: 89054798

Gene position: 2311027-2311917 (Clockwise)

Preceding gene: 89054795

Following gene: 89054799

Centisome position: 53.52

GC content: 66.67

Gene sequence:

>891_bases
ATGGAAAGCAAACCGGATATTTTGTGTATTGGCGCCGTGTTGTGGGACATCATCGGGCGCTGCGATGACACCATGGTGTT
GGGCAATGACAAGCCGGGACGGATCACGCGCATTCCCGGGGGCGTGGCGCTGAACATTGCGATGACGTTGACGGGGTTCG
GGCTACGGCCCGGGTTGCTGTCGGTGGTTGGAGAGGATGCGGAGGGGCGCGAGTTGCGCGCGGCCTGTGCCGGGATGGGG
CTGGATGTCGGCGCGATGCTGGTTGATGCGACACTGCCCACCGATCGCTACATGGCGATCGAGGCGCAGGGTGCTTTGGT
TGCGGCCATCGCCCATGCGCAGTCCTTGGAAGAGGCCGGGGACCGCATTCTGGAGCCTTTGGCCGACGGGCGTTTGGGGT
CGGCTGAGGTTCCTTGGGCGGGACCCGTCGCGCTGGACGGAAACCTGACGGAGGCGTTGTTGGGCGAAATTGCCCATAGC
GCGCTCTTTGCGCAGGCGGACCTGCGCATTGCTCCCGCCTCCCCCGGAAAGGCCGAGCGGTTGCGCCCGCTTTTGGCGCA
TCCCCGGGCCACGATCTATGTGAACCGGGAGGAAGCCGGACTTCTGACCGGAACGCATCCGGCCAATGCTGCCGCTGCCG
CTGCCGCGCTGATTAATGCGGGCGCGCGGAGGGTCCTGGTGACCGAAGGCGCGCAGATGGCGGTGGATGCCAGCGCCGAT
GGCACGCTCTCCGCTGTGCCGCCTGCGGTCGAGGCGCGCCGGATCACCGGGGCGGGGGATACCTTCATGGCCGCTCATAT
CGCCGCTGAAGTTGGAGGGTCCAGCCGGGACGCGGCCCTGAGGACGGCTGTCGACGCCGCCGCCACTTACGTTTCGGGAG
ACACGCCATGA

Upstream 100 bases:

>100_bases
TCGACCCTTAGAGTGAGTTGTATTTGCCCAGATGAAAGAGGGAGCGGCGTGACACAGCGCACGGCTTGCGATGGCGGCGC
GGGCGGGGCAAGAGAGAGGA

Downstream 100 bases:

>100_bases
CCCTGCCGCTGACCTTTTCGCCCGAGGTGGCGGAGGCCCGCGCGGCCGGCGCGCCTCTTGTCGCGCTGGAATCGACGATC
ATCACCCATGGCATGCCCTA

Product: PfkB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MESKPDILCIGAVLWDIIGRCDDTMVLGNDKPGRITRIPGGVALNIAMTLTGFGLRPGLLSVVGEDAEGRELRAACAGMG
LDVGAMLVDATLPTDRYMAIEAQGALVAAIAHAQSLEEAGDRILEPLADGRLGSAEVPWAGPVALDGNLTEALLGEIAHS
ALFAQADLRIAPASPGKAERLRPLLAHPRATIYVNREEAGLLTGTHPANAAAAAAALINAGARRVLVTEGAQMAVDASAD
GTLSAVPPAVEARRITGAGDTFMAAHIAAEVGGSSRDAALRTAVDAAATYVSGDTP

Sequences:

>Translated_296_residues
MESKPDILCIGAVLWDIIGRCDDTMVLGNDKPGRITRIPGGVALNIAMTLTGFGLRPGLLSVVGEDAEGRELRAACAGMG
LDVGAMLVDATLPTDRYMAIEAQGALVAAIAHAQSLEEAGDRILEPLADGRLGSAEVPWAGPVALDGNLTEALLGEIAHS
ALFAQADLRIAPASPGKAERLRPLLAHPRATIYVNREEAGLLTGTHPANAAAAAAALINAGARRVLVTEGAQMAVDASAD
GTLSAVPPAVEARRITGAGDTFMAAHIAAEVGGSSRDAALRTAVDAAATYVSGDTP
>Mature_296_residues
MESKPDILCIGAVLWDIIGRCDDTMVLGNDKPGRITRIPGGVALNIAMTLTGFGLRPGLLSVVGEDAEGRELRAACAGMG
LDVGAMLVDATLPTDRYMAIEAQGALVAAIAHAQSLEEAGDRILEPLADGRLGSAEVPWAGPVALDGNLTEALLGEIAHS
ALFAQADLRIAPASPGKAERLRPLLAHPRATIYVNREEAGLLTGTHPANAAAAAAALINAGARRVLVTEGAQMAVDASAD
GTLSAVPPAVEARRITGAGDTFMAAHIAAEVGGSSRDAALRTAVDAAATYVSGDTP

Specific function: Catalyzes the phosphorylation of pseudouridine to pseudouridine 5'-phosphate (PsiMP) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788491, Length=297, Percent_Identity=26.9360269360269, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.83 [H]

Molecular weight: Translated: 30090; Mature: 30090

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESKPDILCIGAVLWDIIGRCDDTMVLGNDKPGRITRIPGGVALNIAMTLTGFGLRPGLL
CCCCCCEEEHHHHHHHHHHCCCCEEEECCCCCCEEEECCCCEEEEEEEEEECCCCCCCHH
SVVGEDAEGRELRAACAGMGLDVGAMLVDATLPTDRYMAIEAQGALVAAIAHAQSLEEAG
HHHCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHH
DRILEPLADGRLGSAEVPWAGPVALDGNLTEALLGEIAHSALFAQADLRIAPASPGKAER
HHHHHHHHCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHH
LRPLLAHPRATIYVNREEAGLLTGTHPANAAAAAAALINAGARRVLVTEGAQMAVDASAD
HHHHHHCCCEEEEEECCCCCEEECCCCCHHHHHHHHHHHCCCCEEEEECCCEEEEECCCC
GTLSAVPPAVEARRITGAGDTFMAAHIAAEVGGSSRDAALRTAVDAAATYVSGDTP
CCCCCCCCCHHHEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MESKPDILCIGAVLWDIIGRCDDTMVLGNDKPGRITRIPGGVALNIAMTLTGFGLRPGLL
CCCCCCEEEHHHHHHHHHHCCCCEEEECCCCCCEEEECCCCEEEEEEEEEECCCCCCCHH
SVVGEDAEGRELRAACAGMGLDVGAMLVDATLPTDRYMAIEAQGALVAAIAHAQSLEEAG
HHHCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHH
DRILEPLADGRLGSAEVPWAGPVALDGNLTEALLGEIAHSALFAQADLRIAPASPGKAER
HHHHHHHHCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHH
LRPLLAHPRATIYVNREEAGLLTGTHPANAAAAAAALINAGARRVLVTEGAQMAVDASAD
HHHHHHCCCEEEEEECCCCCEEECCCCCHHHHHHHHHHHCCCCEEEEECCCEEEEECCCC
GTLSAVPPAVEARRITGAGDTFMAAHIAAEVGGSSRDAALRTAVDAAATYVSGDTP
CCCCCCCCCHHHEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 3076173 [H]