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The map label for this gene is livK [C]

Identifier: 89054754

GI number: 89054754

Start: 2271424

End: 2272533

Strand: Reverse

Name: livK [C]

Synonym: Jann_2263

Alternate gene names: 89054754

Gene position: 2272533-2271424 (Counterclockwise)

Preceding gene: 89054755

Following gene: 89054748

Centisome position: 52.63

GC content: 60.9

Gene sequence:

>1110_bases
ATGAAACATTTGAAACTTCTGGCGACCGCATCCGCGCTTGCCCTGGCGACACCCGCGATGGCAGACGATGTCATCATCGG
CGTCGTGGTCTCGACCACATCGACCTATGCCTTTGTGGGGCAACCCCTGGTCAATGGCATGCGCCTCGCCGCGGATGAGC
TGACCGCCGGAGACGGCTGGGGCGGCCACACCGTCGAGATCCTCTATGAGGACAACCGATCCGAGCGTCAGGAAGCTATC
GCCCTGATCACCCGCATGGCGGTGTCTGAAGACGCCGACATCATCATCGGGCCTATCGCAAGCTCGGAAGCCATGGCGAC
CGGCCCCGTGGCCGTTGATCTGGAAATCCCGATGTTCACCACCGCGACCTCGCCTGCCGTGTTGGATGCCGGACCTTGGG
TCTTCAAATCTACTGAAACGGCTGACGCCTATATGGCCCCGGTGGCCGAGCATGTTGTGGGTCTTGCGCCCGAGGATTGC
TTCCTCGTCTCGATCCGCGACAACGAAGGCTACATCCTGCAGCGCGATGTGTTCCGGGGCATCCTGGAAGATGGCGGCGT
GACGATCGCGGGCGACGAGACGGTGCTGGCGGCAGACACCGACTTCACGGCGCTGTCCACGCTGATCGTGAATTCCGACG
CCGATTGTCTGTTCGTGACCGCTCCGCCCGAGCAGGCCGCCAACATTATCATCCAGTCCCGTCAGGCGGGTCTCAACCCG
GATACGATCCTGGCCAGCGATTCCGGTGCAGGCTCGCAAAACTACATCGACGCGGGCGGCAGTGCTATTGAAGGCACGTT
TTTCCCGGCCAGCTTTGTCGCGGATGCGTCCGAAAGCGCAATGGCATTCTCGACCGCCTATGTCGATGCCTATGACATTG
ATCCCGATATCTGGGCGGCCACGGGCTACACGATGATGCAGACTGTCGCTAATGCGATCCGCAACGCAGGCGATGATGTA
AACCGGGAAACGTTGCGAGAGGCCATGGCCGCCACGCGTGATCTTCCGGTCGTGATGGGTCAGGGAACGCTGACTTTTGA
CGAGAACCGCATACCCATGATGGGCGGTATCGTCATGCAAATTCGTGACGGCGCACGCGTCCTTCCGTAA

Upstream 100 bases:

>100_bases
CGCATCGTGAAATCCGGTGTCAGCGCAGAGCTGGCCGATGATCCAGAGGTGACGGCACTTTATCTCGGGCACGCGTAAAC
GAAATCTAGGGAGGACTACC

Downstream 100 bases:

>100_bases
CGCCCAAACACGCAGGTCGGCGCCTCTGATCTACCGAGGCGCCGGTTCCTGATACGCGAAATCGTTGGCCCTGTAGCGAC
CTCTCGCCTCAGGTCGCAGA

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MKHLKLLATASALALATPAMADDVIIGVVVSTTSTYAFVGQPLVNGMRLAADELTAGDGWGGHTVEILYEDNRSERQEAI
ALITRMAVSEDADIIIGPIASSEAMATGPVAVDLEIPMFTTATSPAVLDAGPWVFKSTETADAYMAPVAEHVVGLAPEDC
FLVSIRDNEGYILQRDVFRGILEDGGVTIAGDETVLAADTDFTALSTLIVNSDADCLFVTAPPEQAANIIIQSRQAGLNP
DTILASDSGAGSQNYIDAGGSAIEGTFFPASFVADASESAMAFSTAYVDAYDIDPDIWAATGYTMMQTVANAIRNAGDDV
NRETLREAMAATRDLPVVMGQGTLTFDENRIPMMGGIVMQIRDGARVLP

Sequences:

>Translated_369_residues
MKHLKLLATASALALATPAMADDVIIGVVVSTTSTYAFVGQPLVNGMRLAADELTAGDGWGGHTVEILYEDNRSERQEAI
ALITRMAVSEDADIIIGPIASSEAMATGPVAVDLEIPMFTTATSPAVLDAGPWVFKSTETADAYMAPVAEHVVGLAPEDC
FLVSIRDNEGYILQRDVFRGILEDGGVTIAGDETVLAADTDFTALSTLIVNSDADCLFVTAPPEQAANIIIQSRQAGLNP
DTILASDSGAGSQNYIDAGGSAIEGTFFPASFVADASESAMAFSTAYVDAYDIDPDIWAATGYTMMQTVANAIRNAGDDV
NRETLREAMAATRDLPVVMGQGTLTFDENRIPMMGGIVMQIRDGARVLP
>Mature_369_residues
MKHLKLLATASALALATPAMADDVIIGVVVSTTSTYAFVGQPLVNGMRLAADELTAGDGWGGHTVEILYEDNRSERQEAI
ALITRMAVSEDADIIIGPIASSEAMATGPVAVDLEIPMFTTATSPAVLDAGPWVFKSTETADAYMAPVAEHVVGLAPEDC
FLVSIRDNEGYILQRDVFRGILEDGGVTIAGDETVLAADTDFTALSTLIVNSDADCLFVTAPPEQAANIIIQSRQAGLNP
DTILASDSGAGSQNYIDAGGSAIEGTFFPASFVADASESAMAFSTAYVDAYDIDPDIWAATGYTMMQTVANAIRNAGDDV
NRETLREAMAATRDLPVVMGQGTLTFDENRIPMMGGIVMQIRDGARVLP

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=358, Percent_Identity=23.7430167597765, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI48994941, Length=242, Percent_Identity=24.7933884297521, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 38783; Mature: 38783

Theoretical pI: Translated: 3.82; Mature: 3.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLKLLATASALALATPAMADDVIIGVVVSTTSTYAFVGQPLVNGMRLAADELTAGDGW
CCCEEEEHHHHHHHHCCCCCCCCEEEEEEEECCCCEEEECCHHHCCEEEEHHHCCCCCCC
GGHTVEILYEDNRSERQEAIALITRMAVSEDADIIIGPIASSEAMATGPVAVDLEIPMFT
CCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEECCCEEEEEECCEEE
TATSPAVLDAGPWVFKSTETADAYMAPVAEHVVGLAPEDCFLVSIRDNEGYILQRDVFRG
CCCCCCEECCCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEHHHHHH
ILEDGGVTIAGDETVLAADTDFTALSTLIVNSDADCLFVTAPPEQAANIIIQSRQAGLNP
HHHCCCEEEECCCEEEEECCCHHEEEEEEECCCCCEEEEECCCHHHHHEEEEECCCCCCC
DTILASDSGAGSQNYIDAGGSAIEGTFFPASFVADASESAMAFSTAYVDAYDIDPDIWAA
CEEEECCCCCCCCCEECCCCCEEECCCCCHHHHCCCCCCCEEEEEEEEEEEECCCCCEEC
TGYTMMQTVANAIRNAGDDVNRETLREAMAATRDLPVVMGQGTLTFDENRIPMMGGIVMQ
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEECCCEEEECCCCCCEECCEEEE
IRDGARVLP
EECCCCCCC
>Mature Secondary Structure
MKHLKLLATASALALATPAMADDVIIGVVVSTTSTYAFVGQPLVNGMRLAADELTAGDGW
CCCEEEEHHHHHHHHCCCCCCCCEEEEEEEECCCCEEEECCHHHCCEEEEHHHCCCCCCC
GGHTVEILYEDNRSERQEAIALITRMAVSEDADIIIGPIASSEAMATGPVAVDLEIPMFT
CCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEECCCEEEEEECCEEE
TATSPAVLDAGPWVFKSTETADAYMAPVAEHVVGLAPEDCFLVSIRDNEGYILQRDVFRG
CCCCCCEECCCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEHHHHHH
ILEDGGVTIAGDETVLAADTDFTALSTLIVNSDADCLFVTAPPEQAANIIIQSRQAGLNP
HHHCCCEEEECCCEEEEECCCHHEEEEEEECCCCCEEEEECCCHHHHHEEEEECCCCCCC
DTILASDSGAGSQNYIDAGGSAIEGTFFPASFVADASESAMAFSTAYVDAYDIDPDIWAA
CEEEECCCCCCCCCEECCCCCEEECCCCCHHHHCCCCCCCEEEEEEEEEEEECCCCCEEC
TGYTMMQTVANAIRNAGDDVNRETLREAMAATRDLPVVMGQGTLTFDENRIPMMGGIVMQ
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEECCCEEEECCCCCCEECCEEEE
IRDGARVLP
EECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA