Click here to switch to the map view.

The map label for this gene is ntrX [H]

Identifier: 89054731

GI number: 89054731

Start: 2246621

End: 2248018

Strand: Direct

Name: ntrX [H]

Synonym: Jann_2240

Alternate gene names: 89054731

Gene position: 2246621-2248018 (Clockwise)

Preceding gene: 89054730

Following gene: 89054733

Centisome position: 52.03

GC content: 61.59

Gene sequence:

>1398_bases
ATGAGCGATATTCTGGTCGTAGATGATGAACGCGATATCCGGGAGTTGATCTGTGATATCCTGCAAGATGAAGGGTTTAC
GACCCGGATGGCGGGCAATTCCGACGAGGCGATGGCGGAGTTGAACAAAAGCGCGCCGGGCCTGATGATCCTCGATATCT
GGCTCAAGGACAGCAACATGGACGGGATCGACATCTTGGGCCATGTGAAGCGGGACAACCCCGAAGTGCCGGTCGTCATC
ATCTCGGGCCACGGCAACGTCGAAATCGCGGTCGCGGCGATCAAACAGGGCGCGTATGACTTCATCGAAAAGCCGTTTAA
TATCGATCAGTTGATGGTCGTAATCAAACGCGCGATGGAGACGTCGCGTTTGCGCCGCGAGAACGTGTCGCTGCGGCGTC
AGGACACGCGCACGGCGGATATGATCGGCAGCTCGTCTGCCTTCCGGACGCTTAAAAGCCAGCTCGACAAGGTCACGAAA
TCCAATGGCCGCGTCATGCTGACAGGGGGGGCTGGCACCGGCAAGGAAGTGGCCGCGCGCTACGTCCATGCGGAGAGCAA
CCGCGCCGATGCGCCGTTTGTGACCGTGGCCAGTGCCTCCATCCAGCCCGATCATATGGAGGAGGTCCTGTTTGGTCGCG
AAAGTCAGCAGCGCGGGGTAGAGCAGGGGCTGTTGGAGCAAGCCCATGGCGGTGTGATCTATTTCGACGAGGTCGCGGAT
ATGCCGCTGGCCACCCAAGCCAAGATCCTGCGCGTCCTGGTCGACCAGAGCTTCACGCGTGTGGGGGGGACCGGCAAGGT
CCGGGTGGATCTGCGTGTGATTTCGTCGACGACCCGGGATTTACAGGTCGAAATAACCGAAGGCCGCTTCCGGGAGGAGC
TGTATCATCGCCTCAACGTTGTGCCGGTTGAGGTTCCGTCGCTGGAAGATCGCCGCGAGGACGTGCCCGAGCTGGCGCGC
CATTTCATCGACCTCTTCAACCGCGAGCAAGGGCTTGCGATCCGGGAGCTTGGCGACGACGCAGCGGCGATGCTGCAGAC
AATGTCCTGGCCGGGCAATGTGCGGCAGCTGAAGAACGTGATCGAGCGGGTGATGATCCTGGGCGACGGCTCTGGTCCCA
TCGAGGCGCGCGATCTGCCGGGGCAAGTGGAAGCGCCCACGTCGGAGGAAGACATGGCGCTGTCGGCGTCCCTCACGACG
CTGCCGCTGCGCGAGGCGCGGGAGCTGTTTGAACGGCAGTATCTGATGGCGCAGATCAACCGGTTTGGCGGCAATATTTC
CCGCACGGCCAGCTTTGTGGGCATGGAACGATCCGCCCTGCACCGGAAGCTGAAATCCCTGGGGGTTGTGACCACGTCCA
AGGCCGGAACGCGGGTGGCTCAGGTCAGCGAAGGGTAG

Upstream 100 bases:

>100_bases
TGACGCTGGACGATGCCGAACCGTTTGAGGACGGCGCGCATCCCGGCGCCAGGGCCCGCGTTACGCTGCCACGCCTCCCA
GACCCCACAGGAGAAGAGAC

Downstream 100 bases:

>100_bases
GAGTGCTCAAGGTTGAGCAGACTGGTTAAGTCGTTCATACCAAACGATAAACGAATATTCGTTAACGGTTTCTTAACCCT
TTCTCGGGCTGTCCCGAACC

Product: two component sigma54 specific Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MSDILVVDDERDIRELICDILQDEGFTTRMAGNSDEAMAELNKSAPGLMILDIWLKDSNMDGIDILGHVKRDNPEVPVVI
ISGHGNVEIAVAAIKQGAYDFIEKPFNIDQLMVVIKRAMETSRLRRENVSLRRQDTRTADMIGSSSAFRTLKSQLDKVTK
SNGRVMLTGGAGTGKEVAARYVHAESNRADAPFVTVASASIQPDHMEEVLFGRESQQRGVEQGLLEQAHGGVIYFDEVAD
MPLATQAKILRVLVDQSFTRVGGTGKVRVDLRVISSTTRDLQVEITEGRFREELYHRLNVVPVEVPSLEDRREDVPELAR
HFIDLFNREQGLAIRELGDDAAAMLQTMSWPGNVRQLKNVIERVMILGDGSGPIEARDLPGQVEAPTSEEDMALSASLTT
LPLREARELFERQYLMAQINRFGGNISRTASFVGMERSALHRKLKSLGVVTTSKAGTRVAQVSEG

Sequences:

>Translated_465_residues
MSDILVVDDERDIRELICDILQDEGFTTRMAGNSDEAMAELNKSAPGLMILDIWLKDSNMDGIDILGHVKRDNPEVPVVI
ISGHGNVEIAVAAIKQGAYDFIEKPFNIDQLMVVIKRAMETSRLRRENVSLRRQDTRTADMIGSSSAFRTLKSQLDKVTK
SNGRVMLTGGAGTGKEVAARYVHAESNRADAPFVTVASASIQPDHMEEVLFGRESQQRGVEQGLLEQAHGGVIYFDEVAD
MPLATQAKILRVLVDQSFTRVGGTGKVRVDLRVISSTTRDLQVEITEGRFREELYHRLNVVPVEVPSLEDRREDVPELAR
HFIDLFNREQGLAIRELGDDAAAMLQTMSWPGNVRQLKNVIERVMILGDGSGPIEARDLPGQVEAPTSEEDMALSASLTT
LPLREARELFERQYLMAQINRFGGNISRTASFVGMERSALHRKLKSLGVVTTSKAGTRVAQVSEG
>Mature_464_residues
SDILVVDDERDIRELICDILQDEGFTTRMAGNSDEAMAELNKSAPGLMILDIWLKDSNMDGIDILGHVKRDNPEVPVVII
SGHGNVEIAVAAIKQGAYDFIEKPFNIDQLMVVIKRAMETSRLRRENVSLRRQDTRTADMIGSSSAFRTLKSQLDKVTKS
NGRVMLTGGAGTGKEVAARYVHAESNRADAPFVTVASASIQPDHMEEVLFGRESQQRGVEQGLLEQAHGGVIYFDEVADM
PLATQAKILRVLVDQSFTRVGGTGKVRVDLRVISSTTRDLQVEITEGRFREELYHRLNVVPVEVPSLEDRREDVPELARH
FIDLFNREQGLAIRELGDDAAAMLQTMSWPGNVRQLKNVIERVMILGDGSGPIEARDLPGQVEAPTSEEDMALSASLTTL
PLREARELFERQYLMAQINRFGGNISRTASFVGMERSALHRKLKSLGVVTTSKAGTRVAQVSEG

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=454, Percent_Identity=35.2422907488987, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1790299, Length=470, Percent_Identity=35.9574468085106, Blast_Score=300, Evalue=2e-82,
Organism=Escherichia coli, GI1790437, Length=454, Percent_Identity=33.4801762114537, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1788905, Length=451, Percent_Identity=33.4811529933481, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI87082117, Length=338, Percent_Identity=36.3905325443787, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI1789233, Length=322, Percent_Identity=31.6770186335404, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1789087, Length=338, Percent_Identity=34.3195266272189, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1787583, Length=340, Percent_Identity=33.8235294117647, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI87082152, Length=324, Percent_Identity=34.8765432098765, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1786524, Length=325, Percent_Identity=33.8461538461538, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI87081872, Length=320, Percent_Identity=30.625, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI87081858, Length=296, Percent_Identity=28.3783783783784, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI1789828, Length=240, Percent_Identity=29.5833333333333, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1786599, Length=118, Percent_Identity=38.135593220339, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789809, Length=113, Percent_Identity=30.0884955752212, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI1786911, Length=139, Percent_Identity=32.3741007194245, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51438; Mature: 51307

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDILVVDDERDIRELICDILQDEGFTTRMAGNSDEAMAELNKSAPGLMILDIWLKDSNM
CCCEEEECCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCEEEEEEEEECCCC
DGIDILGHVKRDNPEVPVVIISGHGNVEIAVAAIKQGAYDFIEKPFNIDQLMVVIKRAME
CCCHHEEEECCCCCCCCEEEEECCCCEEEEEEEHHHCCHHHHHCCCCHHHHHHHHHHHHH
TSRLRRENVSLRRQDTRTADMIGSSSAFRTLKSQLDKVTKSNGRVMLTGGAGTGKEVAAR
HHHHHHHCCCHHHCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
YVHAESNRADAPFVTVASASIQPDHMEEVLFGRESQQRGVEQGLLEQAHGGVIYFDEVAD
HHCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEECCCCC
MPLATQAKILRVLVDQSFTRVGGTGKVRVDLRVISSTTRDLQVEITEGRFREELYHRLNV
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCEEEEECCCHHHHHHHHHCCE
VPVEVPSLEDRREDVPELARHFIDLFNREQGLAIRELGDDAAAMLQTMSWPGNVRQLKNV
EEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHCCCCCCHHHHHHH
IERVMILGDGSGPIEARDLPGQVEAPTSEEDMALSASLTTLPLREARELFERQYLMAQIN
HHHHEEEECCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHCCHHHHHHHHHHHHHHHHHH
RFGGNISRTASFVGMERSALHRKLKSLGVVTTSKAGTRVAQVSEG
HHCCCHHHHHHHHCCHHHHHHHHHHHCCCEEECCCCCEEEECCCC
>Mature Secondary Structure 
SDILVVDDERDIRELICDILQDEGFTTRMAGNSDEAMAELNKSAPGLMILDIWLKDSNM
CCEEEECCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCEEEEEEEEECCCC
DGIDILGHVKRDNPEVPVVIISGHGNVEIAVAAIKQGAYDFIEKPFNIDQLMVVIKRAME
CCCHHEEEECCCCCCCCEEEEECCCCEEEEEEEHHHCCHHHHHCCCCHHHHHHHHHHHHH
TSRLRRENVSLRRQDTRTADMIGSSSAFRTLKSQLDKVTKSNGRVMLTGGAGTGKEVAAR
HHHHHHHCCCHHHCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
YVHAESNRADAPFVTVASASIQPDHMEEVLFGRESQQRGVEQGLLEQAHGGVIYFDEVAD
HHCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEECCCCC
MPLATQAKILRVLVDQSFTRVGGTGKVRVDLRVISSTTRDLQVEITEGRFREELYHRLNV
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCEEEEECCCHHHHHHHHHCCE
VPVEVPSLEDRREDVPELARHFIDLFNREQGLAIRELGDDAAAMLQTMSWPGNVRQLKNV
EEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHCCCCCCHHHHHHH
IERVMILGDGSGPIEARDLPGQVEAPTSEEDMALSASLTTLPLREARELFERQYLMAQIN
HHHHEEEECCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHCCHHHHHHHHHHHHHHHHHH
RFGGNISRTASFVGMERSALHRKLKSLGVVTTSKAGTRVAQVSEG
HHCCCHHHHHHHHCCHHHHHHHHHHHCCCEEECCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]