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The map label for this gene is ispDF

Identifier: 89054726

GI number: 89054726

Start: 2239442

End: 2240590

Strand: Reverse

Name: ispDF

Synonym: Jann_2235

Alternate gene names: 89054726

Gene position: 2240590-2239442 (Counterclockwise)

Preceding gene: 89054732

Following gene: 89054725

Centisome position: 51.89

GC content: 65.36

Gene sequence:

>1149_bases
ATGGAAAAACCTGCGCAACGTGCCACCGCTATTATCGTCGCTGCCGGACGCGGTATCCGCGCTGGAGGGGGCGAACCCAA
GCAATGGCGGCGCATTGCAGGCCGCAGTATCGTGGAATGGTCGGTGAACCGTTTCGCGGCGCATCACGCGGGCTTTGATG
TGGTCCTTGTGATCCATCCAGACGATGAATGGCGGCTGAACGGCCTCGACCTGCCGACGACATTGCGGGTTGTGCATGGG
GGCGATAGCCGCGCGGCATCGGTCAAAGCAGGGCTGGCGGTGTGTTGCGATGCGGGCCACGTGCTGATCCACGACGTGGC
GCGGCCTTGCGTGAGTTTGGCGGTCATCCAATCGGTGTTGGACGCCACACGCGCCGGGGGGGCCGCCGCACCCGCCCTGC
CCGTCACCGATGCGTTGTGGCGGGGGGACACCCATGTGTCCGGCACCCAGAACCGCGACGGATTGTGGCGCGCGCAGACG
CCGCAAGGCTTTGACGTGTCCGCCATTCGTGCAGCACATGCGGCACATGATGGCACAGCCGCCGATGACGTGGAGGTTGC
GCGCCTGGCGGGGATAGACGTGGCCATCGTGCCGGGTGACGAGGCCAACCTGAAGATCACCGGTCCAGAGGATTTCGCAC
GCGCCGAGGCGCTGCTGACGGGAGGCGACATGGATATCAGGGTCGGCAATGGATTTGACGTGCACCGCTTCGGGCCGGGT
GATCAGGTCTGGTTGTGCGGTATCTCGGTTCCGCACACCCGCGGCTTGCAGGGGCATTCCGACGCGGATGTCGGGCTGCA
TACGTTGACCGATGCGATCTATGGCGCGTTGGCCGAGGGCGATATCGGCACCCATTTCCCGCCAAGTGACCCCCAATGGA
AGGACGCAGAGAGCCATATTTTCCTGACCCACGCGGTGGAGCTTGCGGCGAACCGGGGCTTCAGCATCACCAATGCGGAT
CTGACATTGATCTGTGAGCAGCCCAAGATCGGGCCCGTCGCGAGCGCCATGCGCGCCCGCGTGGCCGACCTTCTGCGGAT
TGACGTGGCCCGCGTCTCCGTCAAGGCCACAACGTCCGAGCGGCTTGGGTTCACCGGCCGGGAGGAAGGGATCGCGGCGC
TGGCCACGATCACGCTGGTGGCCCGATGA

Upstream 100 bases:

>100_bases
AGATTTGGTCTACTGCACAATAATTAAGCATAGCGCTTATGCATTGCGCCAGAACTATATCGCGTTGTTGTCCCGAGGGG
GACCGATTATCAAAACCATC

Downstream 100 bases:

>100_bases
CCCGTTGGATCACAACGGTCGGTGGCGTGGGCCTGTTGCGTCCGGCCCCCGGAACCTGGGGAAGTGCCGCGGGCCTGGTG
GCGGGCGCGCTGATCCACAT

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MEKPAQRATAIIVAAGRGIRAGGGEPKQWRRIAGRSIVEWSVNRFAAHHAGFDVVLVIHPDDEWRLNGLDLPTTLRVVHG
GDSRAASVKAGLAVCCDAGHVLIHDVARPCVSLAVIQSVLDATRAGGAAAPALPVTDALWRGDTHVSGTQNRDGLWRAQT
PQGFDVSAIRAAHAAHDGTAADDVEVARLAGIDVAIVPGDEANLKITGPEDFARAEALLTGGDMDIRVGNGFDVHRFGPG
DQVWLCGISVPHTRGLQGHSDADVGLHTLTDAIYGALAEGDIGTHFPPSDPQWKDAESHIFLTHAVELAANRGFSITNAD
LTLICEQPKIGPVASAMRARVADLLRIDVARVSVKATTSERLGFTGREEGIAALATITLVAR

Sequences:

>Translated_382_residues
MEKPAQRATAIIVAAGRGIRAGGGEPKQWRRIAGRSIVEWSVNRFAAHHAGFDVVLVIHPDDEWRLNGLDLPTTLRVVHG
GDSRAASVKAGLAVCCDAGHVLIHDVARPCVSLAVIQSVLDATRAGGAAAPALPVTDALWRGDTHVSGTQNRDGLWRAQT
PQGFDVSAIRAAHAAHDGTAADDVEVARLAGIDVAIVPGDEANLKITGPEDFARAEALLTGGDMDIRVGNGFDVHRFGPG
DQVWLCGISVPHTRGLQGHSDADVGLHTLTDAIYGALAEGDIGTHFPPSDPQWKDAESHIFLTHAVELAANRGFSITNAD
LTLICEQPKIGPVASAMRARVADLLRIDVARVSVKATTSERLGFTGREEGIAALATITLVAR
>Mature_382_residues
MEKPAQRATAIIVAAGRGIRAGGGEPKQWRRIAGRSIVEWSVNRFAAHHAGFDVVLVIHPDDEWRLNGLDLPTTLRVVHG
GDSRAASVKAGLAVCCDAGHVLIHDVARPCVSLAVIQSVLDATRAGGAAAPALPVTDALWRGDTHVSGTQNRDGLWRAQT
PQGFDVSAIRAAHAAHDGTAADDVEVARLAGIDVAIVPGDEANLKITGPEDFARAEALLTGGDMDIRVGNGFDVHRFGPG
DQVWLCGISVPHTRGLQGHSDADVGLHTLTDAIYGALAEGDIGTHFPPSDPQWKDAESHIFLTHAVELAANRGFSITNAD
LTLICEQPKIGPVASAMRARVADLLRIDVARVSVKATTSERLGFTGREEGIAALATITLVAR

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=155, Percent_Identity=45.8064516129032, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789104, Length=222, Percent_Identity=38.2882882882883, Blast_Score=109, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPDF_JANSC (Q28Q60)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_510177.1
- ProteinModelPortal:   Q28Q60
- SMR:   Q28Q60
- STRING:   Q28Q60
- GeneID:   3934689
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_2235
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   IVLIHDA
- PhylomeDB:   Q28Q60
- ProtClustDB:   CLSK933757
- BioCyc:   JSP290400:JANN_2235-MONOMER
- HAMAP:   MF_01520
- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00453
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF01128 IspD; PF02542 YgbB; SSF69765 YgbB_synth

EC number: =2.7.7.60; =4.6.1.12

Molecular weight: Translated: 40214; Mature: 40214

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS01295 ISPD; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKPAQRATAIIVAAGRGIRAGGGEPKQWRRIAGRSIVEWSVNRFAAHHAGFDVVLVIHP
CCCCHHHCEEEEEECCCCEECCCCCHHHHHHHHCCEEEEHHHHHHHHHCCCCEEEEEEEC
DDEWRLNGLDLPTTLRVVHGGDSRAASVKAGLAVCCDAGHVLIHDVARPCVSLAVIQSVL
CCCEEECCCCCCEEEEEEECCCCCCHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
DATRAGGAAAPALPVTDALWRGDTHVSGTQNRDGLWRAQTPQGFDVSAIRAAHAAHDGTA
HHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCC
ADDVEVARLAGIDVAIVPGDEANLKITGPEDFARAEALLTGGDMDIRVGNGFDVHRFGPG
CCCCHHHEECCCEEEEECCCCCCEEEECCHHHHHHHHEEECCCEEEEECCCEEEEEECCC
DQVWLCGISVPHTRGLQGHSDADVGLHTLTDAIYGALAEGDIGTHFPPSDPQWKDAESHI
CEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEE
FLTHAVELAANRGFSITNADLTLICEQPKIGPVASAMRARVADLLRIDVARVSVKATTSE
EEEEEHHHHHCCCCEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHEEHEEEEEECCHH
RLGFTGREEGIAALATITLVAR
HCCCCCCHHCCEEEEEEEEEEC
>Mature Secondary Structure
MEKPAQRATAIIVAAGRGIRAGGGEPKQWRRIAGRSIVEWSVNRFAAHHAGFDVVLVIHP
CCCCHHHCEEEEEECCCCEECCCCCHHHHHHHHCCEEEEHHHHHHHHHCCCCEEEEEEEC
DDEWRLNGLDLPTTLRVVHGGDSRAASVKAGLAVCCDAGHVLIHDVARPCVSLAVIQSVL
CCCEEECCCCCCEEEEEEECCCCCCHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
DATRAGGAAAPALPVTDALWRGDTHVSGTQNRDGLWRAQTPQGFDVSAIRAAHAAHDGTA
HHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCC
ADDVEVARLAGIDVAIVPGDEANLKITGPEDFARAEALLTGGDMDIRVGNGFDVHRFGPG
CCCCHHHEECCCEEEEECCCCCCEEEECCHHHHHHHHEEECCCEEEEECCCEEEEEECCC
DQVWLCGISVPHTRGLQGHSDADVGLHTLTDAIYGALAEGDIGTHFPPSDPQWKDAESHI
CEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEE
FLTHAVELAANRGFSITNADLTLICEQPKIGPVASAMRARVADLLRIDVARVSVKATTSE
EEEEEHHHHHCCCCEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHEEHEEEEEECCHH
RLGFTGREEGIAALATITLVAR
HCCCCCCHHCCEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA