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The map label for this gene is nodE [H]

Identifier: 89054639

GI number: 89054639

Start: 2155412

End: 2156617

Strand: Direct

Name: nodE [H]

Synonym: Jann_2148

Alternate gene names: 89054639

Gene position: 2155412-2156617 (Clockwise)

Preceding gene: 89054638

Following gene: 89054641

Centisome position: 49.92

GC content: 64.26

Gene sequence:

>1206_bases
ATGCGCCGTGTCGTCATCACCGGGCAGGGCACGATCAACGCCTTGGGCACCGATGTGCCGACCACGCTGGAGGCGTTTCG
CGAAGGGCGCTGTGGTATTTCGGATCTGGAGTTGCGCGACGTCGACCGGCTGAGCGTCAGGATCGGCGGACAGGTGAAGG
GCTACGACCCGGAGGCGCTGTTCAACCGTCAGCAAATCGCGCTCTATGATCGGTTCACGCAGTTCACCCTCATCGCCGCG
CGTCAGGCCTTGGATGGCATGAGTTTCGCGGGCGACGAGGCTGCGCGCGCTGGCGTCGTGCTTGGCACTGCGGGCGGTGG
CGTGAACACCTGGGATGAAAATTACCGCACCGTTTATGAAGAGGGGAAGAACCGCGTTCACCCCTTCGTGGTGCCGAAAC
TGATGAACAACGCCGCTGCCTCTCACGTGTCGATGGAATGGAATCTGAAGGGGCCGTCGTTTTCCGTGGCCACGGCCTGC
GCCTCCTCCAATCACGCCATGGGGCAGGCCTTCAACATCATCCGCTATGGCGGCGCAGATGTGATGGTCACCGGCGGGTC
CGAGGCGATGTTGTCGTTCGGCGGTGTGAAGGCCTGGGAGGGCCTGCGCGTCATGTCCAAGGACGCCTGCCGCCCGTTTT
CGGCCAACCGCAACGGCATGGTTCAGGGGGAGGGGGCAGCGGTCTTCGTCTTTGAAGAGCTGGACCACGCGAAGGCCCGT
GGTGCCGAGATCCTGGCCGAAGTCGTGGGCTTTGCAATGACATCGGACGCGGCAGACATCGTCATGCCCTCGCAACAGGG
GGCGGCGCGGGCGATTTCGGCGGCCCTGACCGATGGGGGCCTCAACGCCGAGGACGTGTTCTATATCAACGCCCACGGCA
CCGGGACGGCTGCCAACGACAAGGTGGAATGTGCTGCCGTGGCCCATGCCTTCGGGCACCACGCCGACGATCTTATGATC
TCGTCAACCAAGTCCATGCACGGCCACCTGATTGGCGGTACGGGTGCGGTGGAGCTGCTGGCCTGTATCATGGCGCTCAA
GGACGGCGTTGTTGCGCCGACCATCGGCTACGAGGAAGCGGACCCCGAATGCGCCCTCGACGTCGTCCCGAATGAGGCGC
GGGAGGCGAAGGTTGAGGCCTGCCTGTCCAATGCCTTCGCGTTTGGTGGACTGAATGCGGTGCTGGCGCTGCGTGCCGCC
CCCTAG

Upstream 100 bases:

>100_bases
CAACGCCAATGAGCCGGAGGCCTCGGAGTTCGATATCTCCTCCGTCGCGGCGATTGTAAGCGCGGTCGAAAAACTGGTGG
CGGAGCAAAAGGGCTGATCC

Downstream 100 bases:

>100_bases
ACCTTGCACTTTGCCATGAAAAAGGCCCGCGCGGATCGCTCCGGCGGGCCCGATATATGGTGAAGCCGCGTCTATTCCGT
CGCTTCGCCGTCAGCGTCAG

Product: 3-oxoacyl-[acyl-carrier-protein] synthase II

Products: [acyl carrier protein]; acetoacetyl-ACP; CO2; [acyl carrier protein]; beta-ketoacyl-ACP [C]

Alternate protein names: Host-specificity of nodulation protein B [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MRRVVITGQGTINALGTDVPTTLEAFREGRCGISDLELRDVDRLSVRIGGQVKGYDPEALFNRQQIALYDRFTQFTLIAA
RQALDGMSFAGDEAARAGVVLGTAGGGVNTWDENYRTVYEEGKNRVHPFVVPKLMNNAAASHVSMEWNLKGPSFSVATAC
ASSNHAMGQAFNIIRYGGADVMVTGGSEAMLSFGGVKAWEGLRVMSKDACRPFSANRNGMVQGEGAAVFVFEELDHAKAR
GAEILAEVVGFAMTSDAADIVMPSQQGAARAISAALTDGGLNAEDVFYINAHGTGTAANDKVECAAVAHAFGHHADDLMI
SSTKSMHGHLIGGTGAVELLACIMALKDGVVAPTIGYEEADPECALDVVPNEAREAKVEACLSNAFAFGGLNAVLALRAA
P

Sequences:

>Translated_401_residues
MRRVVITGQGTINALGTDVPTTLEAFREGRCGISDLELRDVDRLSVRIGGQVKGYDPEALFNRQQIALYDRFTQFTLIAA
RQALDGMSFAGDEAARAGVVLGTAGGGVNTWDENYRTVYEEGKNRVHPFVVPKLMNNAAASHVSMEWNLKGPSFSVATAC
ASSNHAMGQAFNIIRYGGADVMVTGGSEAMLSFGGVKAWEGLRVMSKDACRPFSANRNGMVQGEGAAVFVFEELDHAKAR
GAEILAEVVGFAMTSDAADIVMPSQQGAARAISAALTDGGLNAEDVFYINAHGTGTAANDKVECAAVAHAFGHHADDLMI
SSTKSMHGHLIGGTGAVELLACIMALKDGVVAPTIGYEEADPECALDVVPNEAREAKVEACLSNAFAFGGLNAVLALRAA
P
>Mature_401_residues
MRRVVITGQGTINALGTDVPTTLEAFREGRCGISDLELRDVDRLSVRIGGQVKGYDPEALFNRQQIALYDRFTQFTLIAA
RQALDGMSFAGDEAARAGVVLGTAGGGVNTWDENYRTVYEEGKNRVHPFVVPKLMNNAAASHVSMEWNLKGPSFSVATAC
ASSNHAMGQAFNIIRYGGADVMVTGGSEAMLSFGGVKAWEGLRVMSKDACRPFSANRNGMVQGEGAAVFVFEELDHAKAR
GAEILAEVVGFAMTSDAADIVMPSQQGAARAISAALTDGGLNAEDVFYINAHGTGTAANDKVECAAVAHAFGHHADDLMI
SSTKSMHGHLIGGTGAVELLACIMALKDGVVAPTIGYEEADPECALDVVPNEAREAKVEACLSNAFAFGGLNAVLALRAA
P

Specific function: Proposed to synthesize NOD factor fatty acyl chain. Involved in the synthesis of a highly unsaturated fatty acid moiety, which forms part of a lipo-oligosaccharide that is responsible for host specificity [H]

COG id: COG0304

COG function: function code IQ; 3-oxoacyl-(acyl-carrier-protein) synthase

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-ketoacyl-ACP synthases family [H]

Homologues:

Organism=Homo sapiens, GI8923559, Length=416, Percent_Identity=37.2596153846154, Blast_Score=242, Evalue=6e-64,
Organism=Homo sapiens, GI223972627, Length=187, Percent_Identity=36.8983957219251, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI41872631, Length=272, Percent_Identity=27.2058823529412, Blast_Score=92, Evalue=9e-19,
Organism=Escherichia coli, GI1787337, Length=408, Percent_Identity=40.4411764705882, Blast_Score=279, Evalue=2e-76,
Organism=Escherichia coli, GI1788663, Length=416, Percent_Identity=33.1730769230769, Blast_Score=197, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI71983541, Length=409, Percent_Identity=32.0293398533007, Blast_Score=197, Evalue=9e-51,
Organism=Caenorhabditis elegans, GI71983535, Length=261, Percent_Identity=34.0996168582375, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI212642053, Length=284, Percent_Identity=28.5211267605634, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17550940, Length=232, Percent_Identity=25.8620689655172, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320904, Length=440, Percent_Identity=32.5, Blast_Score=199, Evalue=9e-52,
Organism=Drosophila melanogaster, GI24644341, Length=415, Percent_Identity=36.144578313253, Blast_Score=245, Evalue=3e-65,
Organism=Drosophila melanogaster, GI19920632, Length=275, Percent_Identity=28.7272727272727, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI221330659, Length=275, Percent_Identity=28.7272727272727, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24581345, Length=276, Percent_Identity=27.8985507246377, Blast_Score=99, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000794
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615 [H]

Pfam domain/function: PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C [H]

EC number: 2.3.1.41 [C]

Molecular weight: Translated: 42104; Mature: 42104

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00606 B_KETOACYL_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRVVITGQGTINALGTDVPTTLEAFREGRCGISDLELRDVDRLSVRIGGQVKGYDPEAL
CCEEEEECCCCEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEECCEECCCCHHHH
FNRQQIALYDRFTQFTLIAARQALDGMSFAGDEAARAGVVLGTAGGGVNTWDENYRTVYE
HCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCEEEECCCCCCCCCCCHHHHHHH
EGKNRVHPFVVPKLMNNAAASHVSMEWNLKGPSFSVATACASSNHAMGQAFNIIRYGGAD
CCCCCCCCEEHHHHHCCHHHCEEEEEEEECCCCEEHHHHHCCCCCCCHHHEEEEEECCCE
VMVTGGSEAMLSFGGVKAWEGLRVMSKDACRPFSANRNGMVQGEGAAVFVFEELDHAKAR
EEEECCCHHHHCCCCCCHHCCCCHHHHCCCCCCCCCCCCEEECCCCEEEEEECCCHHHHH
GAEILAEVVGFAMTSDAADIVMPSQQGAARAISAALTDGGLNAEDVFYINAHGTGTAAND
HHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCC
KVECAAVAHAFGHHADDLMISSTKSMHGHLIGGTGAVELLACIMALKDGVVAPTIGYEEA
CCHHHHHHHHHCCCCCCEEEECCCCCCCEEECCCCHHHHHHHHHHHHCCCEECCCCCCCC
DPECALDVVPNEAREAKVEACLSNAFAFGGLNAVLALRAAP
CCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCC
>Mature Secondary Structure
MRRVVITGQGTINALGTDVPTTLEAFREGRCGISDLELRDVDRLSVRIGGQVKGYDPEAL
CCEEEEECCCCEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEECCEECCCCHHHH
FNRQQIALYDRFTQFTLIAARQALDGMSFAGDEAARAGVVLGTAGGGVNTWDENYRTVYE
HCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCEEEECCCCCCCCCCCHHHHHHH
EGKNRVHPFVVPKLMNNAAASHVSMEWNLKGPSFSVATACASSNHAMGQAFNIIRYGGAD
CCCCCCCCEEHHHHHCCHHHCEEEEEEEECCCCEEHHHHHCCCCCCCHHHEEEEEECCCE
VMVTGGSEAMLSFGGVKAWEGLRVMSKDACRPFSANRNGMVQGEGAAVFVFEELDHAKAR
EEEECCCHHHHCCCCCCHHCCCCHHHHCCCCCCCCCCCCEEECCCCEEEEEECCCHHHHH
GAEILAEVVGFAMTSDAADIVMPSQQGAARAISAALTDGGLNAEDVFYINAHGTGTAAND
HHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCC
KVECAAVAHAFGHHADDLMISSTKSMHGHLIGGTGAVELLACIMALKDGVVAPTIGYEEA
CCHHHHHHHHHCCCCCCEEEECCCCCCCEEECCCCHHHHHHHHHHHHCCCEECCCCCCCC
DPECALDVVPNEAREAKVEACLSNAFAFGGLNAVLALRAAP
CCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: None [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} [C]

Substrates: acetyl-ACP; malonyl-ACP; acyl-ACP [C]

Specific reaction: acetyl-ACP + malonyl-ACP = [acyl carrier protein] + acetoacetyl-ACP + CO2 acyl-ACP + malonyl-ACP = [acyl carrier protein] + beta-ketoacyl-ACP + CO2 [C]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl-ACP-decarboxylation, Cerulenin [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9100384 [H]