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The map label for this gene is norM [H]

Identifier: 89054421

GI number: 89054421

Start: 1918247

End: 1919629

Strand: Direct

Name: norM [H]

Synonym: Jann_1930

Alternate gene names: 89054421

Gene position: 1918247-1919629 (Clockwise)

Preceding gene: 89054420

Following gene: 89054423

Centisome position: 44.42

GC content: 66.74

Gene sequence:

>1383_bases
ATGATCTTCACCGACCATATCCGCGCCACGCTCAAGCTTGGATTGCCCCTGATCGGCGGGCAATTGGCGCAGACATTCAT
CGGGGTGACGGATACCGTGATGATGGGCTGGTACGGGGTTGAAGAGCTGGCCGCCGTGGCTCTGGGGTCGTCCTATTTCC
ACCTGATCCTGATCCTCGGCATGGGGTTCGGCCTTGCGGTCATGCCCATGGTGGCCTCGGCCGCCGCGCAGGACGATGAA
TTGCAGGTGCGCCGGGTCACGCGGATGGGTCTGTGGATTTCCATCGCCTTCTCGGCCCTGGTGATGCCGCTCTTCTGGTT
TTCCGAGGCGTTGCTGCTGCTGGCGGGGCAAACGGCACAGGTCTCTGCCGACGCGCAGGTCTATCTGCGGATCGCCGGTT
GGGCGATTTTGCCCGGGGTTCTGCTGGTGGTGCTGCGCTCCCATCTCAGCGCGTTGGAGCGGCCCAACATCGTGCTGTGG
GCGTGGCTTGGCGGCGTGGTCCTGAACGCCTGCGTGAACTGGGTTCTGATCTTCGGCAACCTCGGCGCGCCGGAGCTGGG
CATTGCGGGGGCGGCGGCGGCGTCGGTGTTCACGCACGTGTTGATCTTCGCCATTCTGGCGCTCTATGCGGCGCGCGCGG
ATGGGCTGCGGCACTACGCGCTTTTCGCGCGCTTCTGGCGCCCGGATTGGGAGGCGATGGGCCAGGTCTTTCGCCTTGGC
TGGCCCATCGCGGTGACTCTGCTGCTGGAGGCCGGGCTCTTCATGGCGACGATGATCATGATGGGCTGGATCGGCACGCT
AGAGCTGGCCGCCCACGGCATCGCGCTGCAGATCATATCAGTGACGTTCATGGTGCATATCGGCCTCAGCTCCGCCGCCA
CGGTGCGCACCGGCCGCGCCTGGAGCAAGAACGACGCCCAGGGCCTGCGCCAGGCGGCGGTGGCGGCGCTGCTGTTGTCG
GGCGTGACCGTTGCGGCCACCATCCTGCTCTTCGTGGCCCTGGCCGAGCCCCTTGTGGGCCTGTTCGTGGACCCGTCCGA
CCCGCTGCGCCCGCAGATCATCGCGATCGGCGCGAGCCTTCTTATCGTCGCCTGCGTGTTTCAACTGGCCGATGCGGCGC
AGGTCATGGCCCTGGGCCTGCTGCGCGGCGTCCAGGATACGGGCGTGCCGATGGTCTACGCCTTCATCAGTTACTGGATC
ATCGGGGTGCCGCTGGCCTATTGGCTTGGCTTCCCGCTGGGGTGGGAGGGTGAGGGGATCTGGATTGGCCTCGCCATGGG
GCTCGCCGTGGCGGGTCTCGTGCTGGTGATCCGGTTCTGGCGGATCGCGGCGCGGCTGGGCGCGGCGCCGTCTGGTGGTG
ACGGCGAGGTCGGTGCCAGATAA

Upstream 100 bases:

>100_bases
ACGCGCCGTGGGCGGTGCTGCGAGACGCAGCCCCGCGGCACGGCGCGATCCTCCAACGCGACCGCTTGCCCATCCCGCCT
GCGCCCCGTAAGGCCGACAC

Downstream 100 bases:

>100_bases
GCAAATCCTGCGCCCGGTGGACCTGACGGATGCCTCCGGCGGGGATATTTGAAGAACGGGGAAAGGGGGAGGTAAGCCCC
CGGTGTCCCGAGGGCCTTCC

Product: MATE efflux family protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MIFTDHIRATLKLGLPLIGGQLAQTFIGVTDTVMMGWYGVEELAAVALGSSYFHLILILGMGFGLAVMPMVASAAAQDDE
LQVRRVTRMGLWISIAFSALVMPLFWFSEALLLLAGQTAQVSADAQVYLRIAGWAILPGVLLVVLRSHLSALERPNIVLW
AWLGGVVLNACVNWVLIFGNLGAPELGIAGAAAASVFTHVLIFAILALYAARADGLRHYALFARFWRPDWEAMGQVFRLG
WPIAVTLLLEAGLFMATMIMMGWIGTLELAAHGIALQIISVTFMVHIGLSSAATVRTGRAWSKNDAQGLRQAAVAALLLS
GVTVAATILLFVALAEPLVGLFVDPSDPLRPQIIAIGASLLIVACVFQLADAAQVMALGLLRGVQDTGVPMVYAFISYWI
IGVPLAYWLGFPLGWEGEGIWIGLAMGLAVAGLVLVIRFWRIAARLGAAPSGGDGEVGAR

Sequences:

>Translated_460_residues
MIFTDHIRATLKLGLPLIGGQLAQTFIGVTDTVMMGWYGVEELAAVALGSSYFHLILILGMGFGLAVMPMVASAAAQDDE
LQVRRVTRMGLWISIAFSALVMPLFWFSEALLLLAGQTAQVSADAQVYLRIAGWAILPGVLLVVLRSHLSALERPNIVLW
AWLGGVVLNACVNWVLIFGNLGAPELGIAGAAAASVFTHVLIFAILALYAARADGLRHYALFARFWRPDWEAMGQVFRLG
WPIAVTLLLEAGLFMATMIMMGWIGTLELAAHGIALQIISVTFMVHIGLSSAATVRTGRAWSKNDAQGLRQAAVAALLLS
GVTVAATILLFVALAEPLVGLFVDPSDPLRPQIIAIGASLLIVACVFQLADAAQVMALGLLRGVQDTGVPMVYAFISYWI
IGVPLAYWLGFPLGWEGEGIWIGLAMGLAVAGLVLVIRFWRIAARLGAAPSGGDGEVGAR
>Mature_460_residues
MIFTDHIRATLKLGLPLIGGQLAQTFIGVTDTVMMGWYGVEELAAVALGSSYFHLILILGMGFGLAVMPMVASAAAQDDE
LQVRRVTRMGLWISIAFSALVMPLFWFSEALLLLAGQTAQVSADAQVYLRIAGWAILPGVLLVVLRSHLSALERPNIVLW
AWLGGVVLNACVNWVLIFGNLGAPELGIAGAAAASVFTHVLIFAILALYAARADGLRHYALFARFWRPDWEAMGQVFRLG
WPIAVTLLLEAGLFMATMIMMGWIGTLELAAHGIALQIISVTFMVHIGLSSAATVRTGRAWSKNDAQGLRQAAVAALLLS
GVTVAATILLFVALAEPLVGLFVDPSDPLRPQIIAIGASLLIVACVFQLADAAQVMALGLLRGVQDTGVPMVYAFISYWI
IGVPLAYWLGFPLGWEGEGIWIGLAMGLAVAGLVLVIRFWRIAARLGAAPSGGDGEVGAR

Specific function: Multidrug efflux pump [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Homo sapiens, GI153792564, Length=453, Percent_Identity=26.2693156732892, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI38261960, Length=487, Percent_Identity=25.6673511293634, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI22907060, Length=427, Percent_Identity=24.5901639344262, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI48994890, Length=455, Percent_Identity=29.8901098901099, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI226510954, Length=444, Percent_Identity=25.4504504504505, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 48981; Mature: 48981

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFTDHIRATLKLGLPLIGGQLAQTFIGVTDTVMMGWYGVEELAAVALGSSYFHLILILG
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHH
MGFGLAVMPMVASAAAQDDELQVRRVTRMGLWISIAFSALVMPLFWFSEALLLLAGQTAQ
HCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VSADAQVYLRIAGWAILPGVLLVVLRSHLSALERPNIVLWAWLGGVVLNACVNWVLIFGN
ECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHEEECC
LGAPELGIAGAAAASVFTHVLIFAILALYAARADGLRHYALFARFWRPDWEAMGQVFRLG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHCC
WPIAVTLLLEAGLFMATMIMMGWIGTLELAAHGIALQIISVTFMVHIGLSSAATVRTGRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEECCCC
WSKNDAQGLRQAAVAALLLSGVTVAATILLFVALAEPLVGLFVDPSDPLRPQIIAIGASL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHH
LIVACVFQLADAAQVMALGLLRGVQDTGVPMVYAFISYWIIGVPLAYWLGFPLGWEGEGI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
WIGLAMGLAVAGLVLVIRFWRIAARLGAAPSGGDGEVGAR
EHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MIFTDHIRATLKLGLPLIGGQLAQTFIGVTDTVMMGWYGVEELAAVALGSSYFHLILILG
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHH
MGFGLAVMPMVASAAAQDDELQVRRVTRMGLWISIAFSALVMPLFWFSEALLLLAGQTAQ
HCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VSADAQVYLRIAGWAILPGVLLVVLRSHLSALERPNIVLWAWLGGVVLNACVNWVLIFGN
ECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHEEECC
LGAPELGIAGAAAASVFTHVLIFAILALYAARADGLRHYALFARFWRPDWEAMGQVFRLG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHCC
WPIAVTLLLEAGLFMATMIMMGWIGTLELAAHGIALQIISVTFMVHIGLSSAATVRTGRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEECCCC
WSKNDAQGLRQAAVAALLLSGVTVAATILLFVALAEPLVGLFVDPSDPLRPQIIAIGASL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHH
LIVACVFQLADAAQVMALGLLRGVQDTGVPMVYAFISYWIIGVPLAYWLGFPLGWEGEGI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
WIGLAMGLAVAGLVLVIRFWRIAARLGAAPSGGDGEVGAR
EHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]