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The map label for this gene is ada [H]

Identifier: 89054159

GI number: 89054159

Start: 1652247

End: 1653050

Strand: Reverse

Name: ada [H]

Synonym: Jann_1668

Alternate gene names: 89054159

Gene position: 1653050-1652247 (Counterclockwise)

Preceding gene: 89054164

Following gene: 89054158

Centisome position: 38.28

GC content: 68.91

Gene sequence:

>804_bases
ATGCGCCGCGCGCTGGATCTGATCGACGCAAACCCGCACCAGAGCCTGGACGACCTGGCCAAATCCTTGGACATGAGCCC
GGCCCACTTCCAGCGCACCTTCAGCGGCTGGGTCGGCGTCAGCCCCAAACGCTACCAGCAATATCTGACGCTGGACCATG
CGCGCCGCCTGCTGGCGGATCGGTTCACGGTGCTCGACACAGTAGGCGAGACGGGGCTGACCAGCGGCGGACGCCTCCAC
GATCTGTTCCTGCGGTGGGAGGCGATGAGCCCCGGCACCTACGCCAGCGGCGGCGCGGGCTTGACGATCCGCTGGGGCTG
GTTCGACAGCCCCTTCGGCCCCGCGCTGGTGATGGCCACCGACAAAGGCATCTGCGGCATCGGCTTCGCGGCGGAAACCG
GGCCGGAGCCCACCATGGCCGACCTGCGCGCGCGCTGGCCCAAGGCCGATTACGTCGAAGATCCCATGGCGCTGAAACCC
ATGGCCGAGGCCGCCTTCACCGGCGCGGGCGACCTCCAACTCACGCTCATCGGCGCGCCCTTCCAGATCAAAGTGTGGGA
GGCGTTGCTGTCCATTCCCACCGGCCACGTCACCACGTACAGCCAGATCGCGGGCGCCATTGGCAAGCCCCGCGCGGTGC
GCGCCGTGGGCACCGCTGTGGGCCGCAATCCGATCAGCTGGCTGATCCCCTGCCACCGCGCCTTGCGCAAATCCGGCGGG
CTTGGCGGCTACCATTGGGGTCTGCCCGTCAAGCGCGCAATGCTGGCCTGGGAAGCTGCCACGACGGAGGACGGCGCGGC
TTAG

Upstream 100 bases:

>100_bases
TGGATAAGTTAACGGCGTTAAGGAGATCCAAGCCATGTCCCATCCCGCCAATCACGACGCCGCCCTGCCCGACCCCGGCG
GCAGCTACCACTTCCATGTC

Downstream 100 bases:

>100_bases
ACAAACGGCGAGGACGGCCCCAGGGGGGGGCGGAGGAGCGTTTGGGCCCGATCCCGATAAGCACAACCCTGCCCGTGCGT
CCCCGCGCGCCCTTTTCCCC

Product: transcriptional regulator Ada / DNA-O6-methylguanine--protein-cysteine S-methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MRRALDLIDANPHQSLDDLAKSLDMSPAHFQRTFSGWVGVSPKRYQQYLTLDHARRLLADRFTVLDTVGETGLTSGGRLH
DLFLRWEAMSPGTYASGGAGLTIRWGWFDSPFGPALVMATDKGICGIGFAAETGPEPTMADLRARWPKADYVEDPMALKP
MAEAAFTGAGDLQLTLIGAPFQIKVWEALLSIPTGHVTTYSQIAGAIGKPRAVRAVGTAVGRNPISWLIPCHRALRKSGG
LGGYHWGLPVKRAMLAWEAATTEDGAA

Sequences:

>Translated_267_residues
MRRALDLIDANPHQSLDDLAKSLDMSPAHFQRTFSGWVGVSPKRYQQYLTLDHARRLLADRFTVLDTVGETGLTSGGRLH
DLFLRWEAMSPGTYASGGAGLTIRWGWFDSPFGPALVMATDKGICGIGFAAETGPEPTMADLRARWPKADYVEDPMALKP
MAEAAFTGAGDLQLTLIGAPFQIKVWEALLSIPTGHVTTYSQIAGAIGKPRAVRAVGTAVGRNPISWLIPCHRALRKSGG
LGGYHWGLPVKRAMLAWEAATTEDGAA
>Mature_267_residues
MRRALDLIDANPHQSLDDLAKSLDMSPAHFQRTFSGWVGVSPKRYQQYLTLDHARRLLADRFTVLDTVGETGLTSGGRLH
DLFLRWEAMSPGTYASGGAGLTIRWGWFDSPFGPALVMATDKGICGIGFAAETGPEPTMADLRARWPKADYVEDPMALKP
MAEAAFTGAGDLQLTLIGAPFQIKVWEALLSIPTGHVTTYSQIAGAIGKPRAVRAVGTAVGRNPISWLIPCHRALRKSGG
LGGYHWGLPVKRAMLAWEAATTEDGAA

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=74, Percent_Identity=45.945945945946, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1788542, Length=266, Percent_Identity=33.0827067669173, Blast_Score=140, Evalue=7e-35,
Organism=Escherichia coli, GI1787596, Length=81, Percent_Identity=46.9135802469136, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115533070, Length=126, Percent_Identity=33.3333333333333, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI115533068, Length=126, Percent_Identity=33.3333333333333, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6320001, Length=138, Percent_Identity=35.5072463768116, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17137554, Length=173, Percent_Identity=31.7919075144509, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 28813; Mature: 28813

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRALDLIDANPHQSLDDLAKSLDMSPAHFQRTFSGWVGVSPKRYQQYLTLDHARRLLAD
CCCCHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
RFTVLDTVGETGLTSGGRLHDLFLRWEAMSPGTYASGGAGLTIRWGWFDSPFGPALVMAT
HHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCEEEEEE
DKGICGIGFAAETGPEPTMADLRARWPKADYVEDPMALKPMAEAAFTGAGDLQLTLIGAP
CCCCEEECCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHCHHHHHHCCCCCCEEEEEECCC
FQIKVWEALLSIPTGHVTTYSQIAGAIGKPRAVRAVGTAVGRNPISWLIPCHRALRKSGG
CCHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
LGGYHWGLPVKRAMLAWEAATTEDGAA
CCCEECCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRRALDLIDANPHQSLDDLAKSLDMSPAHFQRTFSGWVGVSPKRYQQYLTLDHARRLLAD
CCCCHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
RFTVLDTVGETGLTSGGRLHDLFLRWEAMSPGTYASGGAGLTIRWGWFDSPFGPALVMAT
HHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCEEEEEE
DKGICGIGFAAETGPEPTMADLRARWPKADYVEDPMALKPMAEAAFTGAGDLQLTLIGAP
CCCCEEECCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHCHHHHHHCCCCCCEEEEEECCC
FQIKVWEALLSIPTGHVTTYSQIAGAIGKPRAVRAVGTAVGRNPISWLIPCHRALRKSGG
CCHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
LGGYHWGLPVKRAMLAWEAATTEDGAA
CCCEECCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]